FastQCFastQC Report
Fri 27 May 2016
SRR939926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358378
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35500.26134109945832457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.164755318475417No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18920.13928376342961973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.12316159419543014No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA14640.10777559707239075No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14480.10659772169455042No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14430.1062296356389753No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA13850.10195983739430409No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA13760.1012972824942689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74400.034.5133631
ATCAACG85200.029.9897333
TCAACGC85750.029.5890064
TATCAAC87900.029.542862
CAACGCA86450.029.487215
ACCGTCC1852.8729119E-828.9431538
AACGCAG89100.028.578616
GGTATCA53250.027.8588161
GTACATG60250.027.0942081
TACCTGG13850.026.6622522
TACATGG59650.026.460032
ACGCAGA98700.025.9194857
CGCAGAG98700.025.798938
ACATGGG58000.025.5697363
TACCGTC1651.1108559E-525.2399867
AGTACTT52200.024.96023412-13
GAGTACT49650.024.92408212-13
AGAGTAC77450.024.69645510-11
GTACCTG15200.024.6933441
GTACTTT51700.023.76449414-15
Produced by FastQC (version 0.11.5)