##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939926_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1358378 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.818757370923265 34.0 31.0 34.0 31.0 34.0 2 32.905279679146744 34.0 31.0 34.0 31.0 34.0 3 32.93047296113453 34.0 31.0 34.0 31.0 34.0 4 36.36428372662101 37.0 37.0 37.0 35.0 37.0 5 36.250824880850544 37.0 37.0 37.0 35.0 37.0 6 36.37191194203675 37.0 37.0 37.0 35.0 37.0 7 36.36227103206913 37.0 37.0 37.0 35.0 37.0 8 36.38891015608321 37.0 37.0 37.0 35.0 37.0 9 38.21293189377331 39.0 39.0 39.0 37.0 39.0 10-11 38.21597780588319 39.0 39.0 39.0 37.0 39.0 12-13 38.19786355491623 39.0 39.0 39.0 37.0 39.0 14-15 39.74767774507538 41.0 40.0 41.0 37.0 41.0 16-17 39.7622311315407 41.0 40.0 41.0 37.0 41.0 18-19 39.79462086400103 41.0 40.0 41.0 37.5 41.0 20-21 39.79554733660292 41.0 40.0 41.0 37.0 41.0 22-23 39.75204140526422 41.0 40.0 41.0 37.0 41.0 24-25 39.708702584994754 41.0 40.0 41.0 37.0 41.0 26-27 39.61323578562079 41.0 40.0 41.0 37.0 41.0 28-29 39.5259669988766 41.0 40.0 41.0 36.5 41.0 30-31 39.398919520192464 41.0 39.0 41.0 36.0 41.0 32-33 39.24786878173822 41.0 39.0 41.0 35.0 41.0 34-35 39.14340706342417 41.0 39.0 41.0 35.0 41.0 36-37 39.12591230128874 41.0 39.0 41.0 35.0 41.0 38-39 39.177978810021955 41.0 39.0 41.0 35.0 41.0 40-41 39.118735727463196 41.0 39.0 41.0 35.0 41.0 42-43 38.99134040745654 41.0 39.0 41.0 35.0 41.0 44-45 38.83148100160633 41.0 39.0 41.0 35.0 41.0 46-47 38.63856157858858 41.0 39.0 41.0 35.0 41.0 48-49 38.411377392743404 41.0 39.0 41.0 35.0 41.0 50-51 38.23710152844053 41.0 38.5 41.0 34.5 41.0 52-53 38.0882166083373 41.0 38.0 41.0 34.0 41.0 54-55 37.93019763276496 40.0 38.0 41.0 34.0 41.0 56-57 37.748986659089 40.0 37.0 41.0 34.0 41.0 58-59 37.5373787708576 40.0 37.0 41.0 33.5 41.0 60-61 37.30274673176391 40.0 36.0 41.0 33.0 41.0 62-63 37.04149250061471 39.0 36.0 41.0 33.0 41.0 64-65 36.75636347172878 39.0 35.0 41.0 33.0 41.0 66-67 36.46078558398325 38.5 35.0 41.0 32.5 41.0 68-69 36.12006157343538 37.0 35.0 40.5 32.0 41.0 70-71 35.76252854507361 37.0 35.0 39.5 32.0 41.0 72-73 35.41576203383741 36.5 35.0 39.0 32.0 41.0 74-75 35.06012391248974 36.0 35.0 39.0 31.5 41.0 76-77 34.69780907818 35.5 35.0 37.5 31.0 39.5 78-79 34.361601851620094 35.0 35.0 37.0 31.0 39.0 80-81 34.11605569289255 35.0 35.0 37.0 31.0 39.0 82-83 33.922052992613246 35.0 35.0 36.0 31.0 38.0 84-85 33.6989575066734 35.0 35.0 36.0 31.0 37.0 86-87 33.52558566172303 35.0 35.0 36.0 31.0 37.0 88-89 33.362414953716865 35.0 35.0 35.0 31.0 36.5 90-91 33.24059466510795 35.0 35.0 35.0 31.0 36.0 92-93 33.125521025811665 35.0 34.0 35.0 31.0 36.0 94-95 33.04578843296932 35.0 34.0 35.0 31.0 36.0 96-97 32.99399578026146 35.0 34.0 35.0 31.0 36.0 98-99 32.92958440139637 35.0 34.0 35.0 31.0 35.0 100-101 32.88242153509553 35.0 34.0 35.0 30.0 35.0 102-103 32.04380408104372 34.5 32.5 35.0 28.0 35.0 104-105 32.73695061315775 35.0 34.0 35.0 30.0 35.0 106-107 32.81496866115323 35.0 34.0 35.0 30.0 35.0 108-109 32.82357230461624 35.0 34.0 35.0 31.0 35.0 110-111 32.78815469626275 35.0 34.0 35.0 30.0 35.0 112-113 32.728654689637196 35.0 34.0 35.0 30.0 35.0 114-115 32.69170584329251 35.0 34.0 35.0 30.0 35.0 116-117 32.64735589062838 35.0 34.0 35.0 30.0 35.0 118-119 32.5845096136716 35.0 34.0 35.0 30.0 35.0 120-121 32.501027696267165 35.0 34.0 35.0 30.0 35.0 122-123 32.35907604510673 35.0 34.0 35.0 30.0 35.0 124-125 30.41642090787689 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0874870449599996 1101 2 0.04350810223910884 1101 3 0.0391083029719681 1101 4 0.02246080511455517 1101 5 -0.014722644652394479 1101 6 -0.021970624122253923 1101 7 0.02882956618591237 1101 8 0.03918641505781295 1101 9 -0.03718505304245667 1101 10-11 0.04449922477591883 1101 12-13 0.018137245461822715 1101 14-15 0.04799224097513388 1101 16-17 0.06865536455861587 1101 18-19 0.07610028092926058 1101 20-21 0.1096339023503532 1101 22-23 0.1005223041293064 1101 24-25 0.020639686290969905 1101 26-27 0.09671154031553186 1101 28-29 0.18342356634037316 1101 30-31 0.1608399478573901 1101 32-33 0.0778321497173522 1101 34-35 0.16038407022053036 1101 36-37 0.12071891728096062 1101 38-39 0.08120234716695762 1101 40-41 0.01830828232124304 1101 42-43 0.04125317300464815 1101 44-45 0.12208901628544311 1101 46-47 0.15159698999525517 1101 48-49 0.1554199088778958 1101 50-51 -0.05982646773868083 1101 52-53 -0.03704661817772603 1101 54-55 0.06212798613948678 1101 56-57 -0.015479738297059953 1101 58-59 0.09856223510550421 1101 60-61 0.12448480699890041 1101 62-63 0.06122151863821301 1101 64-65 0.04598072723199209 1101 66-67 0.09582738039807026 1101 68-69 -0.006242959221943067 1101 70-71 -0.07913605314245586 1101 72-73 -0.17867529549921812 1101 74-75 -0.1585979484471025 1101 76-77 -0.019444496047483995 1101 78-79 -0.013810488566122103 1101 80-81 -0.048986791548088604 1101 82-83 -0.037258503825498224 1101 84-85 -0.045501279979994536 1101 86-87 -0.016494126143065557 1101 88-89 0.04578127533775955 1101 90-91 -0.01863390332911763 1101 92-93 0.008592534818717468 1101 94-95 -0.0769101212577894 1101 96-97 -0.08562122841883024 1101 98-99 -0.044237177769119285 1101 100-101 -0.0658653327573262 1101 102-103 -0.002505707274934821 1101 104-105 -0.03135018640992371 1101 106-107 0.0568131798779703 1101 108-109 0.04120529481377133 1101 110-111 0.05288393402353364 1101 112-113 -0.008502056239613864 1101 114-115 -0.1064119147827185 1101 116-117 -0.09171421268965929 1101 118-119 -0.057915178669595946 1101 120-121 -0.0686353731107161 1101 122-123 -0.0882510489231052 1101 124-125 0.055210240273357414 1102 1 0.08980737519152626 1102 2 0.15077351161700392 1102 3 0.1915423088852677 1102 4 0.11755445354297223 1102 5 0.0061038150904551 1102 6 0.0721166060001508 1102 7 0.08309194024165123 1102 8 0.05331727049120616 1102 9 0.1036326195854329 1102 10-11 0.146538073686024 1102 12-13 0.1464385426693653 1102 14-15 0.14015001789045556 1102 16-17 0.2781866417846146 1102 18-19 0.11659442040559753 1102 20-21 0.14252727048864244 1102 22-23 0.17069525682432385 1102 24-25 0.1970890823984277 1102 26-27 0.12108858178921622 1102 28-29 0.1614157493539281 1102 30-31 0.124955719482152 1102 32-33 0.11361748149604267 1102 34-35 -0.013644021399272788 1102 36-37 0.04826372478026286 1102 38-39 -0.05885905736970898 1102 40-41 -0.012255336862040167 1102 42-43 -0.05501670777370293 1102 44-45 -0.0814896277009396 1102 46-47 0.015898116557693243 1102 48-49 0.03614077919010583 1102 50-51 0.03227355024000644 1102 52-53 -0.08733706113751794 1102 54-55 -0.06862010151337472 1102 56-57 -0.12158886085708787 1102 58-59 -0.1102791206255489 1102 60-61 -0.14926027968244426 1102 62-63 -0.16728793235825634 1102 64-65 -0.10649981825614674 1102 66-67 -0.05552305678305913 1102 68-69 -0.06939550015807328 1102 70-71 -0.09697691605266101 1102 72-73 -0.03988112480259076 1102 74-75 -0.015194143670647975 1102 76-77 -0.02175291436401494 1102 78-79 -0.03555527384830981 1102 80-81 -0.013885009774924129 1102 82-83 -0.022131618384044316 1102 84-85 -0.027259290258037083 1102 86-87 0.04204905773451628 1102 88-89 -0.05344151376234407 1102 90-91 0.034719148378840714 1102 92-93 0.006391600758377081 1102 94-95 -0.06007498410040313 1102 96-97 0.01323450748680699 1102 98-99 -0.02920032424329122 1102 100-101 0.031797688032284555 1102 102-103 -0.031162173464068132 1102 104-105 -0.010603508795369976 1102 106-107 0.0204502587329074 1102 108-109 0.059583856026186766 1102 110-111 -0.007408223697972005 1102 112-113 0.052158539782745095 1102 114-115 0.03277313293835249 1102 116-117 0.039321007518708484 1102 118-119 -0.03759469829253703 1102 120-121 0.016182586511213515 1102 122-123 0.018323192364086083 1102 124-125 0.09114904184052719 1103 1 0.06836655892774957 1103 2 0.0985416021255503 1103 3 0.10969464561713238 1103 4 0.05996364408797916 1103 5 -0.03889650338507522 1103 6 0.08627943265721427 1103 7 0.035307157222327135 1103 8 0.08006290078724021 1103 9 0.12194413917497116 1103 10-11 0.1214708918211187 1103 12-13 0.09658517868917471 1103 14-15 0.10627163381334981 1103 16-17 0.14209724130931534 1103 18-19 0.090420536058339 1103 20-21 0.13305070150245513 1103 22-23 0.0970800488488166 1103 24-25 0.16854310441497233 1103 26-27 0.11193790113012625 1103 28-29 0.20434680049890375 1103 30-31 0.0770287206638045 1103 32-33 0.11323887997701831 1103 34-35 0.12326449265977146 1103 36-37 0.12895430603105495 1103 38-39 0.07461829462702241 1103 40-41 0.14063254986609763 1103 42-43 0.12094788872877871 1103 44-45 -0.02907212384628366 1103 46-47 0.057078101358726485 1103 48-49 0.045598953468704906 1103 50-51 0.09310429916268248 1103 52-53 0.05156247410783976 1103 54-55 0.12186945029272067 1103 56-57 0.06676059026679582 1103 58-59 0.11993970501239204 1103 60-61 0.05841006629180612 1103 62-63 0.08237423648210296 1103 64-65 0.02075408252004962 1103 66-67 0.06553553392975431 1103 68-69 0.13591153771196218 1103 70-71 0.1852930293013344 1103 72-73 0.12520368852682395 1103 74-75 0.15014750919541342 1103 76-77 0.10403901898015278 1103 78-79 0.0717348782292504 1103 80-81 0.012524299374767622 1103 82-83 0.09391153175629796 1103 84-85 0.08167526631419975 1103 86-87 0.0029362811549873413 1103 88-89 0.10710310136546752 1103 90-91 0.11762972374332747 1103 92-93 0.06237452058599757 1103 94-95 0.11678120662471514 1103 96-97 0.03817457368579369 1103 98-99 0.11943673774302965 1103 100-101 0.07339814290320135 1103 102-103 0.017170744386817205 1103 104-105 0.0618887983731895 1103 106-107 0.07427523226648702 1103 108-109 0.06399515400068623 1103 110-111 0.101844396586948 1103 112-113 0.05763892718078267 1103 114-115 0.09870154952192678 1103 116-117 0.0638165807087816 1103 118-119 -3.573582441305234E-4 1103 120-121 -0.09481780554315122 1103 122-123 -0.00613847416347113 1103 124-125 0.0018966851215687086 1104 1 0.06720293093238183 1104 2 0.06538284392262739 1104 3 0.07403603588348773 1104 4 0.05301899343619709 1104 5 0.016944649330426387 1104 6 0.013072941580219322 1104 7 0.005173460634154026 1104 8 -0.0409005906163884 1104 9 -0.055200128426065476 1104 10-11 -0.0012269063794292379 1104 12-13 0.01379592341108804 1104 14-15 0.010210152683796991 1104 16-17 0.06470113268918709 1104 18-19 -0.01399141423414818 1104 20-21 0.08881984661632458 1104 22-23 0.0821097055878397 1104 24-25 0.19427932646562596 1104 26-27 0.13133912451700525 1104 28-29 0.12701286339049034 1104 30-31 0.12073250959987547 1104 32-33 0.1006059999699005 1104 34-35 0.10656355433391695 1104 36-37 0.20608688167614986 1104 38-39 0.17454832847802493 1104 40-41 0.2124796190824796 1104 42-43 0.2016724930581404 1104 44-45 0.1540480323433968 1104 46-47 0.15490451107455527 1104 48-49 0.017319267035823316 1104 50-51 -0.03791427434446604 1104 52-53 0.07433209432340959 1104 54-55 0.060295527437354224 1104 56-57 0.06975781389122915 1104 58-59 0.027283738031954385 1104 60-61 0.08743302706621847 1104 62-63 0.009460044303466475 1104 64-65 0.021588294296634558 1104 66-67 0.08990305478969418 1104 68-69 0.046173920050058825 1104 70-71 0.07495901053481901 1104 72-73 0.031246759465446416 1104 74-75 0.045711432644651495 1104 76-77 -0.012612367722937279 1104 78-79 -0.05019830317051799 1104 80-81 -0.05145111411218295 1104 82-83 -0.03754688199789058 1104 84-85 0.013798822450432624 1104 86-87 0.05795064555798746 1104 88-89 0.07491496109169304 1104 90-91 0.07694821262298035 1104 92-93 -0.0419409246733764 1104 94-95 -0.026754255263220728 1104 96-97 -0.06949813320614595 1104 98-99 -0.08370914074971125 1104 100-101 -0.033831012009642336 1104 102-103 -0.0986510702047454 1104 104-105 -0.061488246791171264 1104 106-107 -0.02575687421567352 1104 108-109 -0.04752476315345433 1104 110-111 -0.05512011183126475 1104 112-113 -0.08685972917221818 1104 114-115 -0.07263849709742942 1104 116-117 -0.035079735768334785 1104 118-119 -0.08581349299485197 1104 120-121 -0.13635301503330766 1104 122-123 0.007531834949574545 1104 124-125 0.036136485281918596 1105 1 -0.07735988954637918 1105 2 -0.022667671057405414 1105 3 -0.09045646745967417 1105 4 -0.04214825330255678 1105 5 -0.1468446826567842 1105 6 -0.0688031266823117 1105 7 -0.10919195821332295 1105 8 0.026876626277434923 1105 9 0.019870941474835035 1105 10-11 0.03886271697223265 1105 12-13 0.10230075439313424 1105 14-15 0.049499187992921634 1105 16-17 0.04183832195209902 1105 18-19 -0.011170758354161592 1105 20-21 0.018693116363124318 1105 22-23 0.017584434813734617 1105 24-25 -0.040625957946808455 1105 26-27 -0.008698248066401959 1105 28-29 -0.0019225493462968757 1105 30-31 -0.09221239683581928 1105 32-33 0.005909895751756267 1105 34-35 0.02360173281900302 1105 36-37 0.0325266560236841 1105 38-39 -0.04978370301413548 1105 40-41 0.0809732914258845 1105 42-43 0.018277610091004703 1105 44-45 -0.08674387675797135 1105 46-47 0.007970037080383463 1105 48-49 0.00605524124696899 1105 50-51 -6.355652041207804E-4 1105 52-53 0.01908974817536091 1105 54-55 -0.0481106470093664 1105 56-57 0.06904755768534443 1105 58-59 -0.018026534114838455 1105 60-61 0.006982784852915813 1105 62-63 0.03267001268731917 1105 64-65 0.09386793332352994 1105 66-67 -0.04091072878586033 1105 68-69 -0.10507788437266186 1105 70-71 0.009971453302519251 1105 72-73 0.027907138327790904 1105 74-75 -0.0028277598595778386 1105 76-77 -0.017611614957630195 1105 78-79 -0.10063446424457823 1105 80-81 -0.09490604118251156 1105 82-83 -0.05741900045658355 1105 84-85 -0.21089807881332945 1105 86-87 -0.15435308195868203 1105 88-89 -0.12782244780157015 1105 90-91 -0.10062823733584025 1105 92-93 -0.19483375746206377 1105 94-95 -0.08091616179633832 1105 96-97 -0.060385244978512276 1105 98-99 -0.1386911186626918 1105 100-101 -0.0044315416021731835 1105 102-103 -0.11774651198941655 1105 104-105 -0.04065524556017408 1105 106-107 0.02211634239475302 1105 108-109 0.0633584519514514 1105 110-111 -0.06451727966250331 1105 112-113 -0.0978075935327638 1105 114-115 0.049828447649595375 1105 116-117 -0.09398140690731793 1105 118-119 -0.10082451879255672 1105 120-121 -0.3115941213326252 1105 122-123 -0.3336594825668371 1105 124-125 -0.3735520470028568 1106 1 -0.03463367101497283 1106 2 0.06971253318454274 1106 3 -0.025633963438430385 1106 4 -0.09132717074541574 1106 5 -0.13817375159779743 1106 6 -0.0073584702651530165 1106 7 -0.02700888859898498 1106 8 -0.03040837727622403 1106 9 -0.05501403215325951 1106 10-11 -1.1158445296644004E-4 1106 12-13 -0.002161894767077399 1106 14-15 -0.13232477489938788 1106 16-17 -0.09054861554088944 1106 18-19 -0.08141539980854162 1106 20-21 -0.05281775035590641 1106 22-23 -0.03644429372403124 1106 24-25 -0.016504086103019233 1106 26-27 -0.04577040607335192 1106 28-29 -0.08572908059981188 1106 30-31 -0.06492589700415863 1106 32-33 -0.02859240477567937 1106 34-35 -0.019191818222786594 1106 36-37 0.009646694926907173 1106 38-39 0.04099361341125274 1106 40-41 -0.006951745045896018 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-0.05424349073962276 2216 7 -0.023335449840878653 2216 8 -0.08342174500748456 2216 9 -0.09323162742192181 2216 10-11 -0.05844567835362113 2216 12-13 -0.026309805843574452 2216 14-15 0.007360208256585565 2216 16-17 -0.0755218334302441 2216 18-19 -0.09799809984281893 2216 20-21 -0.15645224806711155 2216 22-23 -0.1402295865830112 2216 24-25 -0.1035254610870524 2216 26-27 -0.05813124760560129 2216 28-29 -0.21198143075283582 2216 30-31 -0.19661772323784277 2216 32-33 -0.2009966899133815 2216 34-35 -0.11247347354178316 2216 36-37 -0.09119489816754367 2216 38-39 -0.07270035052365387 2216 40-41 -0.03810620511407592 2216 42-43 0.019302287561288267 2216 44-45 0.01994712714803626 2216 46-47 0.047939916936975635 2216 48-49 0.10523909570578382 2216 50-51 0.021346213539516157 2216 52-53 0.025837457236022487 2216 54-55 0.09484243483916543 2216 56-57 -0.04761466452325891 2216 58-59 -0.05512161588775655 2216 60-61 -0.07374471673841043 2216 62-63 -0.101819594943656 2216 64-65 -0.021699828314559966 2216 66-67 0.0939945123030057 2216 68-69 0.038930628552542146 2216 70-71 0.021849599544594867 2216 72-73 0.07312781333369855 2216 74-75 0.020219462762817386 2216 76-77 0.028809472412234527 2216 78-79 0.07764204643693517 2216 80-81 0.12838996892020305 2216 82-83 0.09516578396677033 2216 84-85 0.11269841752331189 2216 86-87 0.12470034042728884 2216 88-89 0.11630294926222717 2216 90-91 0.0751985084932727 2216 92-93 0.18291473116676826 2216 94-95 0.19000604792690723 2216 96-97 0.19539791451010302 2216 98-99 0.24381314444105584 2216 100-101 0.19611241919668032 2216 102-103 0.2234026307410346 2216 104-105 0.1854742853604776 2216 106-107 0.1551975165899222 2216 108-109 0.11443641183280562 2216 110-111 0.09470374476975252 2216 112-113 0.08494310710795361 2216 114-115 0.025165179205352217 2216 116-117 -0.003432470358852413 2216 118-119 -0.003144525903692852 2216 120-121 -0.001883133712738072 2216 122-123 -0.021219904207974594 2216 124-125 -0.00477870721228868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 120.0 3 4.0 4 19.0 5 35.0 6 106.0 7 272.0 8 529.0 9 921.0 10 1622.0 11 2431.0 12 3405.0 13 5079.0 14 7969.0 15 8358.0 16 3884.0 17 2817.0 18 2597.0 19 2279.0 20 2253.0 21 2251.0 22 2208.0 23 2432.0 24 3061.0 25 3880.0 26 5105.0 27 6506.0 28 8634.0 29 11041.0 30 13928.0 31 17922.0 32 23830.0 33 31262.0 34 44687.0 35 75859.0 36 186880.0 37 544946.0 38 327126.0 39 2120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46881074394769 18.825911527360546 14.554912528715322 23.150365199976438 2 15.07724653962299 20.13084723103584 40.28216004676165 24.509746182579516 3 19.385841054551825 25.032796467551744 28.481026636179323 27.100335841717104 4 12.643608774582626 16.95860798687847 37.32083411244882 33.076949126090085 5 13.663407349752271 37.70319135919854 33.675554238659835 14.957847052389356 6 31.666001657859592 36.70694018896066 17.67880516321672 13.948252989963027 7 28.57886390975119 32.27731897895873 20.814456653449923 18.32936045784016 8 26.40332808688009 34.59655559792635 19.674494139333824 19.32562217585974 9 26.10046688035289 17.267947507983784 20.23759218715262 36.393993424510704 10-11 24.860789853781494 26.712778033802074 27.10836011772864 21.31807199468778 12-13 26.083314070163095 23.73448333232723 27.757259025101998 22.42494357240768 14-15 22.584545686105045 25.18628835272656 26.28171245411807 25.947453507050323 16-17 22.17804777318815 28.42833764117031 26.732968829713695 22.660645755927845 18-19 22.132425584042146 26.914526000862793 29.118846153279872 21.834202261815193 20-21 22.85776337270189 26.030696063328346 29.23771504085017 21.873825523119596 22-23 22.395791731802777 25.95883974473884 29.137052511698734 22.508316011759653 24-25 22.25389398238193 26.099104961947262 29.238768590186236 22.40823246548457 26-27 21.941388921198666 26.410689807991588 29.192426555789332 22.455494715020414 28-29 21.97871284723398 26.92983838077472 28.86909976457216 22.222349007419144 30-31 22.162903109443764 26.394199552701824 28.923576500797278 22.519320837057137 32-33 21.96424706524988 26.347379006432668 28.910325402796573 22.778048525520877 34-35 22.597134229205714 26.453645450677204 28.800378097996287 22.148842222120795 36-37 22.600962324183698 25.961330351345502 28.964360435754994 22.473346888715806 38-39 22.21509771212431 26.53543417222599 28.616077409969833 22.63339070567986 40-41 22.671377186615214 26.400125738196582 28.533405281887664 22.39509179330054 42-43 22.460022173503987 26.288043534273964 28.383667874479713 22.868266417742337 44-45 22.48380053269414 26.488466391534608 28.47856045960697 22.549172616164277 46-47 22.994115040143466 26.76707072699941 27.782583345725563 22.456230887131564 48-49 22.69835789448887 26.88055165793321 27.789650597992605 22.631439849585313 50-51 22.661733331959145 27.13283784042439 27.74732070160147 22.458108126015 52-53 23.114000668444277 26.89166785681158 27.46315826669749 22.531173208046656 54-55 22.736417992635335 26.902231926606586 27.75155369124058 22.6097963895175 56-57 22.77698107595971 26.987517465683336 27.549106360674276 22.686395097682677 58-59 22.86407023670878 26.716826980413405 27.60568118741617 22.813421595461648 60-61 22.50776293491208 26.985014480505427 27.944025889700807 22.56319669488169 62-63 22.52664574956308 26.818271497329903 28.03026845252205 22.624814300584962 64-65 22.197871284723398 26.965321876532155 27.898383218809492 22.93842361993495 66-67 22.315695631112987 26.98357894488868 27.752253054746173 22.94847236925215 68-69 22.687278504216057 26.730667016103027 27.976601505619204 22.605452974061713 70-71 22.522780845979543 26.829350887602715 27.608257789805197 23.03961047661255 72-73 22.560215197831532 26.81672553589649 27.778055887241994 22.84500337902999 74-75 22.456819824820485 27.34960371855257 27.46529316581982 22.728283290807124 76-77 22.65845736606453 26.854343930776263 27.56117958329714 22.92601911986207 78-79 22.72184178483456 27.075931736232477 27.5090585978277 22.693167881105257 80-81 22.887001997971108 27.162873662559317 27.186762447566142 22.763361891903433 82-83 22.889100088487886 27.100299033111547 27.428079665601178 22.582521212799385 84-85 22.737853528252078 27.227546382523865 27.362560347708815 22.67203974151525 86-87 22.806317534589045 27.158051735231282 27.170677086937513 22.86495364324216 88-89 22.637586886713418 27.31956789641764 27.003639634917526 23.039205581951418 90-91 22.703392123265612 27.305497223689468 27.12601707564411 22.865093577400813 92-93 22.784342797071215 27.07957578818267 27.400841297488622 22.735240117257494 94-95 22.510284400103654 26.90287168131169 27.43954510118024 23.147298817404415 96-97 22.66964718215401 26.735047240164374 27.60369352271606 22.991612054965554 98-99 22.513652512813266 26.90524147536953 27.156758380338612 23.424347631478597 100-101 22.391554326637102 26.71370077209845 27.54947820043935 23.345266700825103 102-103 22.50091745753285 26.45417479065956 27.521811931696256 23.52309582011133 104-105 22.46114023967384 26.702217255397372 27.28215049773769 23.5544920071911 106-107 22.474426494185153 26.719726008537936 27.228306463160862 23.577541034116045 108-109 22.353273754639286 26.534236031895546 27.34818862144855 23.76430159201662 110-111 22.334640834680588 26.773777235930375 27.322765836521107 23.568816092867934 112-113 22.518632390283695 26.696940849753325 27.28147848743938 23.502948272523604 114-115 22.2283041218101 26.93263913787049 27.141354225946785 23.69770251437263 116-117 22.21800552211847 26.862261020771072 27.23042679180818 23.689306665302272 118-119 21.856351785296244 26.982701376793138 27.248975613490835 23.911971224419784 120-121 21.870873962589734 27.287547837648123 27.177489499367073 23.66408870039507 122-123 21.76636748323719 27.22083329652435 27.13043042954613 23.882368790692333 124-125 21.84995141199682 26.996451603404104 27.18539267940752 23.968204305191556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 58.0 1 42.5 2 97.0 3 145.5 4 79.0 5 67.0 6 172.5 7 408.5 8 728.0 9 826.0 10 672.5 11 537.5 12 525.5 13 629.0 14 731.0 15 802.0 16 883.0 17 993.0 18 1137.5 19 1342.0 20 1580.0 21 1831.5 22 2294.0 23 2883.0 24 3750.5 25 4833.0 26 6423.5 27 8650.5 28 10821.0 29 13086.5 30 15465.5 31 18786.5 32 23390.5 33 25831.5 34 26805.5 35 28796.5 36 31907.5 37 35225.5 38 37614.0 39 39902.5 40 42110.5 41 43712.5 42 44449.0 43 44885.0 44 45935.0 45 48181.5 46 49424.0 47 51140.5 48 53838.0 49 52699.0 50 50066.0 51 48106.5 52 46700.5 53 46903.0 54 47579.0 55 45096.0 56 40671.0 57 37232.5 58 34200.0 59 30291.5 60 24736.5 61 20344.5 62 16938.5 63 12340.5 64 8179.5 65 5927.0 66 4399.0 67 3240.0 68 2362.5 69 1739.0 70 1271.5 71 845.5 72 606.5 73 493.5 74 341.5 75 233.5 76 169.5 77 94.5 78 65.5 79 37.0 80 17.5 81 10.5 82 7.5 83 3.5 84 1.5 85 0.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 7.5 99 9.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016048552023074578 2 0.0 3 0.0 4 0.0 5 0.12161563276201469 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.361721111502101E-5 18-19 0.0 20-21 0.004453841272458771 22-23 3.3127745001759447E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.729807167077206E-4 92-93 0.0 94-95 7.3617211115021E-4 96-97 0.0 98-99 0.00147234422230042 100-101 1.4723442223004202E-4 102-103 3.3127745001759447E-4 104-105 0.0023557507556806723 106-107 0.006441505972564337 108-109 4.7851187224763657E-4 110-111 0.0045642670891313025 112-113 0.009202151389377626 114-115 0.004932353144706407 116-117 0.0016563872500879725 118-119 2.9446884446008404E-4 120-121 5.521290833626575E-4 122-123 0.001030640955610294 124-125 0.0013251098000703779 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1358378.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.39068305094728 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22119795059643 47.238315732998764 2 12.993363416113041 15.693561836565125 3 3.973741226213466 7.199308407562202 4 1.6692527119117058 4.032292458279762 5 0.8896572297292684 2.686350389228202 6 0.5293928704616875 1.9182238229689779 7 0.3233404845763411 1.366872690511267 8 0.2371809143898979 1.145881394132335 9 0.17992201527762175 0.9779052058666695 >10 0.9009371245606888 9.793041839007875 >50 0.05384019424275653 2.2298351308066513 >100 0.02329532599519948 2.7398219269781765 >500 0.0032930117540766533 1.4176287255332434 >1k 0.0015855241778887591 1.5609604395607641 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3550 0.26134109945832457 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2238 0.164755318475417 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1892 0.13928376342961973 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1673 0.12316159419543014 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1464 0.10777559707239075 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1448 0.10659772169455042 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1443 0.1062296356389753 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1385 0.10195983739430409 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1376 0.1012972824942689 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.3617211115021E-5 0.0 3 0.0 0.0 0.0 7.3617211115021E-5 0.0 4 0.0 0.0 0.0 7.3617211115021E-5 0.0 5 0.0 0.0 0.0 7.3617211115021E-5 0.0 6 0.0 0.0 0.0 7.3617211115021E-5 0.0 7 7.3617211115021E-5 0.0 0.0 7.3617211115021E-5 0.0 8 7.3617211115021E-5 0.0 0.0 7.3617211115021E-5 0.0 9 7.3617211115021E-5 0.0 0.0 7.3617211115021E-5 0.0 10-11 7.3617211115021E-5 0.0 0.0 7.3617211115021E-5 0.0 12-13 1.47234422230042E-4 0.0 0.0 7.3617211115021E-5 0.0 14-15 1.47234422230042E-4 0.0 0.0 2.576602389025735E-4 0.0 16-17 1.840430277875525E-4 0.0 0.0 2.94468844460084E-4 7.3617211115021E-5 18-19 2.2085163334506302E-4 0.0 0.0 2.94468844460084E-4 2.2085163334506302E-4 20-21 2.2085163334506302E-4 0.0 0.0 3.312774500175945E-4 3.68086055575105E-4 22-23 2.2085163334506302E-4 0.0 0.0 3.68086055575105E-4 4.048946611326155E-4 24-25 2.94468844460084E-4 0.0 0.0 4.4170326669012603E-4 4.4170326669012603E-4 26-27 3.68086055575105E-4 0.0 0.0 6.993635055926995E-4 4.785118722476365E-4 28-29 3.68086055575105E-4 0.0 0.0 0.0013619184056278886 5.521290833626574E-4 30-31 3.68086055575105E-4 0.0 0.0 0.0031287314723883923 5.88937688920168E-4 32-33 3.68086055575105E-4 0.0 0.0 0.006551931789236869 5.88937688920168E-4 34-35 4.4170326669012603E-4 0.0 0.0 0.011410667722828255 6.62554900035189E-4 36-37 5.15320477805147E-4 0.0 0.0 0.01969260397326812 6.62554900035189E-4 38-39 5.15320477805147E-4 0.0 0.0 0.03511540970186502 6.62554900035189E-4 40-41 5.15320477805147E-4 0.0 0.0 0.056206740686318535 6.62554900035189E-4 42-43 5.15320477805147E-4 0.0 0.0 0.07586253605402915 6.993635055926996E-4 44-45 6.62554900035189E-4 0.0 0.0 0.10063472759423371 7.729807167077204E-4 46-47 6.62554900035189E-4 0.0 0.0 0.12592223961224341 8.09789322265231E-4 48-49 6.62554900035189E-4 0.0 0.0 0.15658380804164967 8.09789322265231E-4 50-51 6.62554900035189E-4 0.0 0.0 0.19405496849919537 8.09789322265231E-4 52-53 6.62554900035189E-4 0.0 0.0 0.23218868385677624 9.202151389377625E-4 54-55 6.62554900035189E-4 0.0 0.0 0.27061686805881724 0.001030640955610294 56-57 6.62554900035189E-4 0.0 0.0 0.31416144843335214 0.001030640955610294 58-59 6.62554900035189E-4 0.0 0.0 0.3635954056970887 0.001030640955610294 60-61 6.62554900035189E-4 0.0 0.0 0.4231149208835832 0.001030640955610294 62-63 6.62554900035189E-4 0.0 0.0 0.4821927328033876 0.001104258166725315 64-65 6.62554900035189E-4 0.0 0.0 0.5397982005008914 0.001104258166725315 66-67 6.62554900035189E-4 0.0 0.0 0.6096241252434889 0.001104258166725315 68-69 6.62554900035189E-4 0.0 0.0 0.6901613542033219 0.001104258166725315 70-71 6.62554900035189E-4 0.0 0.0 0.7778026440357544 0.001104258166725315 72-73 6.62554900035189E-4 0.0 0.0 0.873026506613034 0.0011410667722828254 74-75 6.993635055926996E-4 0.0 0.0 0.9716735695071622 0.0012146839833978465 76-77 7.3617211115021E-4 0.0 0.0 1.074553622040404 0.001251492588955357 78-79 7.3617211115021E-4 0.0 0.0 1.1993347948803645 0.001251492588955357 80-81 8.834065333802521E-4 0.0 0.0 1.3358579128931711 0.0012883011945128676 82-83 8.834065333802521E-4 0.0 0.0 1.482871483489868 0.001325109800070378 84-85 8.834065333802521E-4 0.0 0.0 1.633124211375626 0.001325109800070378 86-87 8.834065333802521E-4 0.0 0.0 1.7893399333617004 0.0013619184056278886 88-89 8.834065333802521E-4 0.0 0.0 1.9677144358933965 0.001398727011185399 90-91 8.834065333802521E-4 0.0 0.0 2.1640515379371577 0.001398727011185399 92-93 8.834065333802521E-4 0.0 0.0 2.3774678329596033 0.0015459614334154411 94-95 8.834065333802521E-4 0.0 0.0 2.5864302867095903 0.0015459614334154411 96-97 8.834065333802521E-4 0.0 0.0 2.8038955283433626 0.0015827700389729514 98-99 9.202151389377626E-4 0.0 0.0 3.0340965475000328 0.001619578644530462 100-101 9.938323500527835E-4 0.0 0.0 3.300922129186427 0.001619578644530462 102-103 0.0011042581667253149 0.0 0.0 3.5878084009016638 0.001619578644530462 104-105 0.001325109800070378 0.0 0.0 3.8763510598669884 0.001619578644530462 106-107 0.0013619184056278886 0.0 0.0 4.168022450304702 0.001619578644530462 108-109 0.001398727011185399 0.0 0.0 4.482257515949168 0.001693195855645483 110-111 0.001398727011185399 0.0 0.0 4.827448618867502 0.0018036216723180147 112-113 0.001398727011185399 0.0 0.0 5.193694244164732 0.001840430277875525 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7440 0.0 34.513363 1 ATCAACG 8520 0.0 29.989733 3 TCAACGC 8575 0.0 29.589006 4 TATCAAC 8790 0.0 29.54286 2 CAACGCA 8645 0.0 29.48721 5 ACCGTCC 185 2.8729119E-8 28.943153 8 AACGCAG 8910 0.0 28.57861 6 GGTATCA 5325 0.0 27.858816 1 GTACATG 6025 0.0 27.094208 1 TACCTGG 1385 0.0 26.662252 2 TACATGG 5965 0.0 26.46003 2 ACGCAGA 9870 0.0 25.919485 7 CGCAGAG 9870 0.0 25.79893 8 ACATGGG 5800 0.0 25.569736 3 TACCGTC 165 1.1108559E-5 25.239986 7 AGTACTT 5220 0.0 24.960234 12-13 GAGTACT 4965 0.0 24.924082 12-13 AGAGTAC 7745 0.0 24.696455 10-11 GTACCTG 1520 0.0 24.693344 1 GTACTTT 5170 0.0 23.764494 14-15 >>END_MODULE