FastQCFastQC Report
Fri 27 May 2016
SRR939925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970892
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44530.2259383061070825No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA30060.15251977277293732No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC25320.12846974872291328No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24590.12476584206542012No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA22890.11614030601372373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG401.9444997E-92302.3083110-111
CCGCTCC4000.02082.8818108-109
CGTCGCT453.1141099E-92046.4962110-111
CCGCTCG1550.01897.122108-109
CCGTTCG700.01750.3208108-109
CCGCGCC3450.01491.5778108-109
TTGTTCG2350.01251.2931108-109
CGTTGCG501.1721195E-51227.8978110-111
CGCGTTG250.00542051071227.8978110-111
CTCCGCC2500.01227.8977110-111
CGTCGCG401.1811062E-51225.2246108-109
CCGCGTG401.1811062E-51225.2246108-109
CCGCGCG1650.01188.0967108-109
CGCCGCT904.9774826E-81023.2481110-111
CCGTGCG755.9240847E-8980.17975108-109
CCGCGCT502.3061451E-5980.1796108-109
CCACGCG553.069012E-5891.07245108-109
CGTCGCC1800.0816.8164108-109
CGTCGGT804.7975034E-5767.4361110-111
GGCGTCG1002.7284841E-11735.1347108-109