##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1970892 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.172608138852866 34.0 31.0 34.0 30.0 34.0 2 32.17662763865295 34.0 31.0 34.0 30.0 34.0 3 32.32435922414825 34.0 31.0 34.0 30.0 34.0 4 35.55052230157715 37.0 35.0 37.0 35.0 37.0 5 35.54161415237365 37.0 37.0 37.0 35.0 37.0 6 35.609467185416555 37.0 37.0 37.0 35.0 37.0 7 35.51052569090544 37.0 37.0 37.0 35.0 37.0 8 35.500192298715504 37.0 37.0 37.0 35.0 37.0 9 37.15970332215058 39.0 39.0 39.0 35.0 39.0 10-11 37.16460516355031 39.0 39.0 39.0 35.0 39.0 12-13 37.12680451288046 39.0 39.0 39.0 35.0 39.0 14-15 38.69522936822515 41.0 40.0 41.0 35.5 41.0 16-17 38.72003463406417 41.0 40.0 41.0 35.0 41.0 18-19 38.765190837448216 41.0 40.0 41.0 35.0 41.0 20-21 38.85210757362656 41.0 40.0 41.0 35.0 41.0 22-23 38.878596594841326 41.0 40.0 41.0 35.0 41.0 24-25 38.92981680376195 41.0 40.0 41.0 35.0 41.0 26-27 38.921381536887864 41.0 39.0 41.0 35.0 41.0 28-29 38.89019946298427 41.0 39.0 41.0 35.0 41.0 30-31 38.82407914791881 41.0 39.0 41.0 34.0 41.0 32-33 38.73729458539585 41.0 39.0 41.0 34.0 41.0 34-35 38.68369448960166 41.0 39.0 41.0 34.0 41.0 36-37 38.84294573218624 41.0 39.0 41.0 35.0 41.0 38-39 38.80707085928605 41.0 39.0 41.0 34.5 41.0 40-41 38.74369016668595 41.0 39.0 41.0 34.0 41.0 42-43 38.68235448720681 41.0 39.0 41.0 34.0 41.0 44-45 38.6068495889171 41.0 39.0 41.0 34.0 41.0 46-47 38.52645984660752 41.0 39.0 41.0 33.0 41.0 48-49 38.45006677179673 41.0 39.0 41.0 33.0 41.0 50-51 38.34502397899023 41.0 39.0 41.0 33.0 41.0 52-53 38.26252224880917 41.0 38.5 41.0 33.0 41.0 54-55 38.13751387696536 40.5 38.0 41.0 33.0 41.0 56-57 37.96036718399587 40.0 38.0 41.0 33.0 41.0 58-59 37.816724609973555 40.0 38.0 41.0 33.0 41.0 60-61 37.6146323593581 40.0 37.0 41.0 32.0 41.0 62-63 37.3852897571252 40.0 37.0 41.0 32.0 41.0 64-65 37.105060804955315 40.0 36.0 41.0 31.0 41.0 66-67 36.80290700860321 39.0 36.0 41.0 31.0 41.0 68-69 36.41348105324899 39.0 35.0 41.0 31.0 41.0 70-71 35.99875361004053 38.5 35.0 40.5 30.5 41.0 72-73 35.596487275812166 37.0 35.0 40.0 30.0 41.0 74-75 35.16640384150932 37.0 35.0 39.0 30.0 41.0 76-77 34.69619415980175 36.5 35.0 39.0 30.0 41.0 78-79 34.22581348952657 36.0 34.5 38.5 29.0 40.0 80-81 33.779688080320994 35.5 34.0 37.0 29.0 39.0 82-83 33.55244807934682 35.0 34.5 37.0 29.0 39.0 84-85 33.12166800616168 35.0 34.0 37.0 29.0 39.0 86-87 32.50485084926014 35.0 34.0 36.0 29.0 37.0 88-89 16.877809387830485 18.5 17.0 18.5 2.0 19.5 90-91 2.904328852113662 2.0 2.0 2.0 2.0 2.0 92-93 2.347078632416185 2.0 2.0 2.0 2.0 2.0 94-95 2.116093372949913 2.0 2.0 2.0 2.0 2.0 96-97 2.0000228323013136 2.0 2.0 2.0 2.0 2.0 98-99 2.0000078644593415 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.046609132903220996 1101 2 0.10830194708942287 1101 3 0.0951858709528679 1101 4 0.07179785668941463 1101 5 0.0703199198638984 1101 6 0.06149086383929614 1101 7 0.12007011066183537 1101 8 0.13620634902719786 1101 9 0.07446622798430269 1101 10-11 0.07658952797003593 1101 12-13 0.030324142447412328 1101 14-15 0.12845159425116748 1101 16-17 0.14572161704443687 1101 18-19 0.10686449175597801 1101 20-21 0.10852878167342084 1101 22-23 0.09682908946560786 1101 24-25 0.13224640925158582 1101 26-27 0.121384425007669 1101 28-29 0.17809259056283366 1101 30-31 0.11438846273929926 1101 32-33 0.20391551449043988 1101 34-35 0.16806454034575324 1101 36-37 0.19521488545432675 1101 38-39 0.12814104267813065 1101 40-41 0.18720184557081865 1101 42-43 0.10606328920537322 1101 44-45 0.11929716888945308 1101 46-47 0.12782074382154462 1101 48-49 0.11093601331908332 1101 50-51 0.10004210928972412 1101 52-53 0.09406706458817382 1101 54-55 0.1130072731985905 1101 56-57 0.12192507553037757 1101 58-59 0.04100467356114734 1101 60-61 0.1201124716111508 1101 62-63 0.06729610682846499 1101 64-65 -0.0044543245126575925 1101 66-67 -0.02025250186375871 1101 68-69 0.08308376747563528 1101 70-71 0.08705105958341619 1101 72-73 -0.0024950127308684955 1101 74-75 0.056422521144149584 1101 76-77 0.05904110935966145 1101 78-79 -0.0032288250035819033 1101 80-81 0.025928886564209108 1101 82-83 0.02839635446233757 1101 84-85 -0.015957632191025084 1101 86-87 0.02303568191345562 1101 88-89 0.7479243514095053 1101 90-91 0.03984672398626321 1101 92-93 -0.1500115741651351 1101 94-95 0.03875568581040856 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.06908399507754126 1102 2 0.08104672999645146 1102 3 0.10806008670153489 1102 4 0.12009281738948374 1102 5 0.09760946601055309 1102 6 0.16904638766441593 1102 7 0.12439365665886726 1102 8 0.09511474529826813 1102 9 0.18841803409599578 1102 10-11 0.1699856848393182 1102 12-13 0.1391901197702623 1102 14-15 0.03884996035233712 1102 16-17 0.08472620578579182 1102 18-19 0.09369321343913128 1102 20-21 0.13066561892266293 1102 22-23 0.11759324325922194 1102 24-25 0.059481329804448535 1102 26-27 0.10331701669667837 1102 28-29 0.15033317404186874 1102 30-31 0.10060801468086567 1102 32-33 0.1149638075363768 1102 34-35 0.1305525952711406 1102 36-37 0.1649815887614423 1102 38-39 0.15179410259354142 1102 40-41 0.12782372937148523 1102 42-43 0.2079707946990439 1102 44-45 0.169216194721173 1102 46-47 0.1320533383416702 1102 48-49 0.09487288834396423 1102 50-51 0.12572680636864675 1102 52-53 0.044063173506195596 1102 54-55 0.15696740444772672 1102 56-57 0.08197720371971684 1102 58-59 -0.0014263787228259162 1102 60-61 0.00725516052797559 1102 62-63 0.006011428842512601 1102 64-65 0.04483665743641296 1102 66-67 0.0883945764533891 1102 68-69 0.07962822681424342 1102 70-71 0.0653455238115157 1102 72-73 0.025826798486420444 1102 74-75 -0.001573064221602749 1102 76-77 0.011609708998228996 1102 78-79 -0.07525856517914775 1102 80-81 -0.10215418044542446 1102 82-83 -0.13188223606604765 1102 84-85 -0.08020384071117093 1102 86-87 0.012213687083601599 1102 88-89 0.8105815760819475 1102 90-91 0.08027992108385806 1102 92-93 -0.05174342705460866 1102 94-95 0.09756422693367384 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.014008081176022813 1103 2 0.09492871252596302 1103 3 0.10399674517665858 1103 4 0.012024908458940331 1103 5 -0.011634726041485521 1103 6 -0.03953407322397595 1103 7 -0.061826614967245064 1103 8 -0.06781568103830438 1103 9 0.08298375020739002 1103 10-11 -0.029766847292272303 1103 12-13 0.009680667466582804 1103 14-15 -0.07315597471267665 1103 16-17 0.005072660801566542 1103 18-19 0.04389896289322337 1103 20-21 0.0178347242348309 1103 22-23 -0.02747473533546696 1103 24-25 -1.317619304259665E-4 1103 26-27 0.027031973428840672 1103 28-29 0.09017077650621985 1103 30-31 0.09847404972593665 1103 32-33 0.1460882669385839 1103 34-35 0.12025070929453818 1103 36-37 0.09138502104189428 1103 38-39 0.12845200919394273 1103 40-41 0.12856821489375392 1103 42-43 0.1613242425538317 1103 44-45 0.1687531683665 1103 46-47 0.09699025225199875 1103 48-49 0.09540798105732762 1103 50-51 0.0446859839793845 1103 52-53 0.051881372503828516 1103 54-55 0.08963040053452431 1103 56-57 0.10843416528992833 1103 58-59 0.11761843774417713 1103 60-61 0.1147097046722223 1103 62-63 0.04206642828600593 1103 64-65 0.08592749387861431 1103 66-67 0.05425113717449648 1103 68-69 0.00496767938216891 1103 70-71 0.1261465093590033 1103 72-73 0.14258791211985056 1103 74-75 0.09559921201034882 1103 76-77 0.11464698572263643 1103 78-79 0.12899771400272897 1103 80-81 0.08578207125514581 1103 82-83 0.06593991857394599 1103 84-85 0.05761866625324785 1103 86-87 0.15007022538407 1103 88-89 0.6156204558824676 1103 90-91 -0.6439657643432835 1103 92-93 -0.37917052192356415 1103 94-95 -0.13001052389689072 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.09445832008476884 1104 2 0.006230976313588599 1104 3 0.06396571712462418 1104 4 0.04859736141932558 1104 5 0.08650820124545078 1104 6 0.05715580685160404 1104 7 -0.0046075982598878795 1104 8 0.02981856446443487 1104 9 0.11181071618180738 1104 10-11 0.1384479562697365 1104 12-13 0.09581559262043982 1104 14-15 0.13604555625045123 1104 16-17 0.1367476684663771 1104 18-19 0.0644825177182824 1104 20-21 0.06807953694848834 1104 22-23 0.007775298666302888 1104 24-25 0.0487552853562363 1104 26-27 0.09764614905314062 1104 28-29 0.1224130949886515 1104 30-31 0.13308938409969073 1104 32-33 0.1270716748452898 1104 34-35 0.1693446486814878 1104 36-37 0.21753695092063907 1104 38-39 0.2247206131544388 1104 40-41 0.25237863334214694 1104 42-43 0.18333433473895866 1104 44-45 0.1302287835295246 1104 46-47 0.20415075518614145 1104 48-49 0.1517131762966173 1104 50-51 0.13500139742165373 1104 52-53 0.14563960880726512 1104 54-55 0.11941575526322623 1104 56-57 0.141970087675233 1104 58-59 0.13603586877822238 1104 60-61 0.14237331079576165 1104 62-63 0.119881870543324 1104 64-65 0.08657045382260975 1104 66-67 0.12545390833762582 1104 68-69 0.18695300598737674 1104 70-71 0.1666034239350438 1104 72-73 0.236213791267204 1104 74-75 0.18973300454041464 1104 76-77 0.11912023497747981 1104 78-79 0.2162560421301407 1104 80-81 0.21883885689341298 1104 82-83 0.230800073345371 1104 84-85 0.27542283606075557 1104 86-87 0.26889890489380264 1104 88-89 0.9388051265801671 1104 90-91 -0.6815079399798849 1104 92-93 -0.37917052192356415 1104 94-95 -0.13001052389689072 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.04305059928824306 1105 2 -0.18805987020698112 1105 3 -0.04090546415177698 1105 4 0.03890550461065345 1105 5 -0.08007859903749193 1105 6 -0.03273414828340293 1105 7 -0.0022195253361303457 1105 8 -0.11024888458346993 1105 9 -0.07355847994170261 1105 10-11 -0.06009678047372091 1105 12-13 -0.07461679569128421 1105 14-15 -0.1605758420756871 1105 16-17 -0.08664992792046888 1105 18-19 -0.07890857305360299 1105 20-21 0.012191565667230009 1105 22-23 -0.16300272363912427 1105 24-25 -0.07616359551467156 1105 26-27 -0.04026125766001343 1105 28-29 -0.051905402251414046 1105 30-31 -0.06829831133013897 1105 32-33 -0.09570406492714056 1105 34-35 0.005170481317925635 1105 36-37 -0.09856534931781624 1105 38-39 -0.07708121205535434 1105 40-41 0.00839388355282722 1105 42-43 0.03303006918119422 1105 44-45 -0.02031774133008213 1105 46-47 0.02350499680873952 1105 48-49 0.002502170795551706 1105 50-51 -0.10935032943061174 1105 52-53 -0.11227344237440917 1105 54-55 -0.14233917210909652 1105 56-57 -0.1038129858232466 1105 58-59 -0.1699662724574722 1105 60-61 -0.28298898625794067 1105 62-63 -0.3178352797201711 1105 64-65 -0.23495379023371044 1105 66-67 -0.1384400894840212 1105 68-69 -0.17266942088910753 1105 70-71 -0.2896953117262768 1105 72-73 -0.26104544932289286 1105 74-75 -0.3110607720433052 1105 76-77 -0.2750012527420864 1105 78-79 -0.2852436642015945 1105 80-81 -0.39070515740260703 1105 82-83 -0.3393623282336904 1105 84-85 -0.359165754317992 1105 86-87 -0.1537702061419992 1105 88-89 0.819985166937748 1105 90-91 -0.9068001822753153 1105 92-93 -0.37917052192356415 1105 94-95 -0.13001052389689072 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.050802720584101735 1106 2 0.13650747222049375 1106 3 0.12287941041699924 1106 4 0.1631218884211023 1106 5 0.11819495729531582 1106 6 0.1335562406658255 1106 7 0.010496803450131154 1106 8 0.0986689810522492 1106 9 0.10462694199812006 1106 10-11 0.06459472522727339 1106 12-13 0.07126123482561297 1106 14-15 0.10759499845465115 1106 16-17 0.09569484406407724 1106 18-19 0.03242957928205925 1106 20-21 0.07252128016347115 1106 22-23 0.022694516539054632 1106 24-25 -0.059608133526815266 1106 26-27 -0.08865867822183304 1106 28-29 0.04710775092157604 1106 30-31 0.10352535768263493 1106 32-33 0.1295008403594835 1106 34-35 0.11618135936041085 1106 36-37 0.10809700313362924 1106 38-39 0.09106319294179599 1106 40-41 0.08129345484264405 1106 42-43 -0.21099484242125044 1106 44-45 0.08414208862126316 1106 46-47 -0.08070638697778776 1106 48-49 -0.050496049291631095 1106 50-51 -0.028446054769013074 1106 52-53 -0.09137059116459056 1106 54-55 -0.08709728099956493 1106 56-57 -0.22152874044407866 1106 58-59 -0.13404684222095398 1106 60-61 -0.06372077205595872 1106 62-63 -0.10783959792265563 1106 64-65 -0.11231498574182552 1106 66-67 -0.15008775286997178 1106 68-69 -0.12648110180624172 1106 70-71 -0.183218307051618 1106 72-73 -0.07908895670809102 1106 74-75 -0.19071169142569744 1106 76-77 -0.15468004307123095 1106 78-79 -0.12807173993402188 1106 80-81 -0.09677396634427993 1106 82-83 -0.06179299775842395 1106 84-85 -0.050906205229239276 1106 86-87 0.051117556478871506 1106 88-89 0.5774172001053692 1106 90-91 -0.3468686754260002 1106 92-93 -0.3445814808276735 1106 94-95 -0.13001052389689072 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.05725839638112262 1107 2 -0.08483536005146419 1107 3 -0.0038111563122811276 1107 4 0.1498859879308867 1107 5 0.14232218504076855 1107 6 0.11099129816341957 1107 7 0.09165316941889046 1107 8 0.0860962500310336 1107 9 0.16374294511352616 1107 10-11 0.1341301629878373 1107 12-13 0.16174950623258155 1107 14-15 0.19767666336508682 1107 16-17 0.1820981518938325 1107 18-19 0.11953442648964341 1107 20-21 0.11793979851231029 1107 22-23 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110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11502.0 3 2137.0 4 1444.0 5 1291.0 6 1493.0 7 2131.0 8 2398.0 9 2784.0 10 3340.0 11 4268.0 12 5648.0 13 6938.0 14 7967.0 15 8778.0 16 9427.0 17 10618.0 18 14167.0 19 17236.0 20 16739.0 21 24389.0 22 27438.0 23 33013.0 24 52130.0 25 90251.0 26 168325.0 27 521744.0 28 885456.0 29 36384.0 30 1456.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41317738364152 20.642480663577707 14.945111147642793 22.999230805137977 2 12.947592847038575 21.153487078842097 44.208666603073965 21.690253471045363 3 18.23432816501397 27.42111100676898 29.79190513777814 24.552655690438904 4 11.230807889800673 17.91282232211525 40.7630394667709 30.09333032131317 5 11.901069283889974 40.293823353671876 35.58974588072126 12.215361481716885 6 28.712582931992216 38.28388364253343 20.280411103195913 12.72312232227844 7 26.94292736486829 33.07862632757148 23.352979260152257 16.625467047407977 8 23.921909470432677 37.99127501659147 21.677646466676002 16.409169046299848 9 26.09452978651291 17.54028125336142 22.20831988764478 34.15686907248089 10-11 23.362042218271924 27.522830578171693 30.176497614132597 18.93862958942379 12-13 25.44575831301643 25.327687729437226 29.26777971996925 19.958774237577092 14-15 20.5599903799855 29.085017892922544 26.83283854865642 23.52215317843554 16-17 19.930376490573277 30.46596809759671 29.513807495715128 20.089847916114884 18-19 20.73076054608513 29.064698554614854 30.40157198637071 19.80296891292931 20-21 22.278918079658624 28.528995774660395 28.67730760497705 20.514778540703933 22-23 20.96367587989226 30.14388746552619 28.75476214818707 20.137674506394475 24-25 21.279577977890217 28.822913685782886 29.90069978466603 19.996808551660873 26-27 19.973316650531842 29.83222824994977 29.496948589775595 20.697506509742798 28-29 20.081116399521733 30.075502131924885 28.427550959055424 21.415830509497955 30-31 20.854009250633723 29.02274706072174 29.116004326974792 21.00723936166974 32-33 20.016063996200657 29.997052058814727 29.473627596382705 20.51325634860191 34-35 20.597587588557662 29.973981310990816 29.457184593161163 19.971246507290356 36-37 20.11662754304592 29.27972369836056 29.453782162825465 21.149866595768053 38-39 20.86212740221179 30.09378494610562 28.597000748899482 20.447086902783106 40-41 21.995598947075738 28.645278381565305 29.377129746328055 19.9819929250309 42-43 20.10465312153076 30.04845014338685 29.322839607649737 20.524057127432656 44-45 19.99389108318858 29.967940871367976 28.928638566494975 21.109529478948463 46-47 21.227748653908993 28.97398741280598 28.777654990735158 21.02060894254987 48-49 20.595350626037288 29.289219230509683 28.792844244399358 21.322585899053674 50-51 20.02287290367656 30.357082152183963 28.988969963331307 20.631074980808172 52-53 20.207652169677488 29.883550189457363 29.161643560377737 20.747154080487416 54-55 20.096399639141936 29.197004553346815 29.651273709217 21.055322098294248 56-57 20.738021159962088 29.14018119714322 28.333592099414883 21.788205543479805 58-59 21.203521045293197 29.333240989359133 27.961679280244685 21.50155868510299 60-61 20.368645263160033 28.89123300515706 29.660859143981504 21.079262587701404 62-63 20.659858581799508 28.93717666924418 29.492103068052433 20.910861680903874 64-65 20.67094493254831 29.570240276991335 28.45625229591474 21.30256249454562 66-67 20.91963943229766 29.87051040848509 27.98869750346544 21.221152655751812 68-69 21.48313180357946 28.720444409829398 29.199163725303904 20.597260061287233 70-71 21.966678032078875 28.652914517893418 28.61658578958157 20.763821660446133 72-73 21.182971974111215 29.373324362775836 28.500394745120484 20.94330891799246 74-75 20.489681829344278 30.204521607475193 29.020996584287722 20.284799978892806 76-77 20.926844291823194 28.729453465740384 29.33892369546378 21.00477854697264 78-79 20.742894877908878 28.838701608338795 29.022654549264555 21.395748964487773 80-81 21.220137886804554 29.450878079570064 28.75393476659299 20.575049267032387 82-83 20.688601904481633 29.500108454229146 29.528775887897652 20.282513753391573 84-85 20.69901379629822 29.16132855647522 29.428593463565463 20.711064183661097 86-87 20.022467252381375 30.306004687781048 28.321422763619186 21.350105296218395 88-89 30.219320616779488 18.51382483407243 35.06296536920417 16.20388917994391 90-91 31.60877844821335 16.53909985060403 18.14506359209504 33.70705810908758 92-93 23.170878283364317 18.464604199767347 36.46848518130147 21.896032335566872 94-95 16.911306868170723 7.612101631220394 30.080367130201914 45.39622437040697 96-97 29.279426804742375 7.553437985070016 17.32185949185499 45.845275718332616 98-99 57.5254243455164 14.06480251787436 19.223534120472284 9.186239016136955 100-101 31.868491578037165 7.161431123527184 13.495042561537861 47.47503473689779 102-103 24.078781448448787 2.152171927285149 38.50013863883152 35.268907985434545 104-105 41.559594119916554 4.315271273396883 43.29595741744543 10.829177189241136 106-107 6.802862122487453 2.2314548754075565 8.458520094150948 82.50716290795404 108-109 20.00296691196429 12.619623596994654 23.608839075217606 43.768570415823454 110-111 41.71092811795908 2.9717202962306364 27.13641080069663 28.180940785113656 112-113 27.848074545240575 2.801462660621678 41.112605763988974 28.23785703014877 114-115 29.78434866361666 3.969178906814351 26.438911630146883 39.807560799422106 116-117 21.194613009125515 4.589027187832847 22.83590753555207 51.38045226748956 118-119 22.124511783968494 4.488763747813842 18.514335197003067 54.872389271214594 120-121 26.946719855062913 4.4438672461048805 32.48110429241729 36.12830860641492 122-123 35.286684020446366 1.7572277652071422 36.4434626042855 26.51262561006099 124-125 39.898688764555644 2.1816637596712853 21.38939532134025 36.53025215443282 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 15.0 2 35.0 3 61.5 4 77.5 5 85.5 6 101.5 7 101.0 8 94.0 9 91.5 10 97.5 11 109.0 12 120.5 13 134.0 14 167.0 15 214.5 16 267.0 17 380.0 18 565.0 19 866.5 20 1310.0 21 2066.5 22 3092.5 23 4593.0 24 6730.5 25 9694.0 26 13572.0 27 18384.5 28 24460.0 29 31815.5 30 40290.0 31 49932.0 32 60851.5 33 72679.0 34 83630.5 35 91677.5 36 96048.0 37 96744.0 38 95237.0 39 92872.5 40 90013.0 41 85777.5 42 81756.0 43 79126.5 44 76641.5 45 73096.5 46 68307.0 47 64005.0 48 60395.5 49 56689.5 50 52234.5 51 47310.5 52 41796.0 53 36144.5 54 30806.5 55 26098.0 56 22001.5 57 18009.0 58 14673.0 59 11786.5 60 9177.5 61 6957.5 62 5219.0 63 3872.0 64 2777.0 65 2000.5 66 1424.0 67 1002.5 68 734.5 69 517.0 70 348.0 71 243.0 72 174.0 73 122.0 74 86.5 75 61.5 76 41.5 77 38.0 78 28.5 79 15.0 80 13.0 81 13.0 82 10.5 83 9.5 84 5.5 85 1.5 86 6.5 87 8.5 88 5.0 89 4.0 90 4.0 91 6.0 92 5.0 93 3.5 94 4.5 95 4.5 96 4.0 97 4.5 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 8.11815157806719E-4 3 0.04449761833728078 4 0.05109361649446038 5 0.05423940023096142 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.86445934125259E-4 12-13 0.0018773225524280376 14-15 5.073844736291994E-5 16-17 3.044306841775196E-4 18-19 0.006951167288720031 20-21 0.0022578609076499374 22-23 5.834921446735792E-4 24-25 0.0 26-27 0.0 28-29 0.0010401381709398588 30-31 0.0 32-33 0.0012684611840729984 34-35 5.073844736291994E-5 36-37 1.2684611840729985E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.2684611840729985E-4 46-47 0.0 48-49 1.522153420887598E-4 50-51 5.073844736291994E-5 52-53 0.0 54-55 0.0017251072103392777 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.3127680258481943E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.805383552218995E-4 80-81 0.0 82-83 7.35707486762339E-4 84-85 5.073844736291994E-5 86-87 0.0011923535130286187 88-89 19.325817954509937 90-91 26.182738577253346 92-93 40.2666660578053 94-95 44.75202091235847 96-97 48.25556144121545 98-99 93.02764940950595 100-101 51.56119665613337 102-103 53.06361282099679 104-105 74.81579406684892 106-107 81.64488465121377 108-109 12.611675322645787 110-111 39.096180815590095 112-113 51.92778193833046 114-115 86.83020175636209 116-117 66.39219703565695 118-119 71.69959084516047 120-121 80.36795014643116 122-123 75.84966603953946 124-125 49.87817698788163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1970892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.393159644049746 #Duplication Level Percentage of deduplicated Percentage of total 1 75.02923489754319 45.31252561138234 2 14.20003904752552 17.151704502978973 3 5.0244726741221 9.103313410062643 4 2.15751010077197 5.211954077982858 5 1.1605038571484432 3.5043247356150706 6 0.6787259651459053 2.459424334057104 7 0.44868655423137216 1.8968319089883685 8 0.29150897806581605 1.4084118600002098 9 0.20224206145486667 1.0992633393766933 >10 0.758356880933213 7.360623112437681 >50 0.028362829036244763 1.1536169082348198 >100 0.016656507838128515 2.020850302886358 >500 0.002606568901867369 0.998137588299967 >1k 0.0010930772814282145 1.3190183076969344 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4453 0.2259383061070825 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3006 0.15251977277293732 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2532 0.12846974872291328 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2459 0.12476584206542012 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 2289 0.11614030601372373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.073844736291994E-5 7 0.0 0.0 0.0 0.0 5.073844736291994E-5 8 0.0 0.0 0.0 0.0 5.073844736291994E-5 9 0.0 0.0 0.0 0.0 1.0147689472583987E-4 10-11 0.0 0.0 0.0 0.0 1.0147689472583987E-4 12-13 0.0 0.0 0.0 5.073844736291994E-5 1.0147689472583987E-4 14-15 0.0 0.0 0.0 1.2684611840729985E-4 1.0147689472583987E-4 16-17 0.0 0.0 0.0 1.522153420887598E-4 1.0147689472583987E-4 18-19 0.0 0.0 0.0 1.522153420887598E-4 1.0147689472583987E-4 20-21 0.0 0.0 0.0 3.044306841775196E-4 1.0147689472583987E-4 22-23 0.0 0.0 0.0 3.8053835522189956E-4 1.522153420887598E-4 24-25 0.0 0.0 0.0 5.073844736291994E-4 2.536922368145997E-4 26-27 0.0 0.0 0.0 8.37184381488179E-4 2.536922368145997E-4 28-29 0.0 0.0 0.0 0.0020295378945167975 2.536922368145997E-4 30-31 0.0 0.0 0.0 0.006037875236187472 3.044306841775196E-4 32-33 0.0 0.0 0.0 0.015196164985194521 3.044306841775196E-4 34-35 0.0 0.0 0.0 0.027322653904932385 3.044306841775196E-4 36-37 0.0 0.0 0.0 0.04690769458701948 3.044306841775196E-4 38-39 0.0 0.0 0.0 0.08049654674127249 3.044306841775196E-4 40-41 0.0 0.0 0.0 0.12182301211837077 3.044306841775196E-4 42-43 0.0 0.0 0.0 0.16048570900891576 3.551691315404396E-4 44-45 0.0 0.0 0.0 0.21046307966139188 4.059075789033595E-4 46-47 0.0 0.0 0.0 0.26893914024715715 4.059075789033595E-4 48-49 0.0 0.0 0.0 0.33185481497717784 4.059075789033595E-4 50-51 0.0 0.0 0.0 0.4021783030221849 4.059075789033595E-4 52-53 0.0 0.0 0.0 0.47103037609366727 4.3127680258481943E-4 54-55 0.0 0.0 0.0 0.5477976469537651 4.566460262662794E-4 56-57 0.0 0.0 0.0 0.641283236219945 4.566460262662794E-4 58-59 0.0 0.0 0.0 0.7393352857487878 4.566460262662794E-4 60-61 0.0 0.0 0.0 0.8468246864871337 5.073844736291994E-4 62-63 0.0 0.0 0.0 0.9599460548827637 5.581229209921194E-4 64-65 0.0 0.0 0.0 1.0796634214355731 5.581229209921194E-4 66-67 0.0 0.0 0.0 1.2176719982627155 5.834921446735792E-4 68-69 0.0 0.0 0.0 1.378741199416305 6.088613683550392E-4 70-71 0.0 0.0 0.0 1.5588373183309892 6.088613683550392E-4 72-73 0.0 0.0 0.0 1.7360667149696685 8.11815157806719E-4 74-75 0.0 0.0 0.0 1.9191310330550837 8.11815157806719E-4 76-77 0.0 0.0 0.0 2.123327914467155 8.11815157806719E-4 78-79 0.0 0.0 0.0 2.1797744371584034 8.11815157806719E-4 80-81 0.0 0.0 0.0 2.1807130984346177 8.11815157806719E-4 82-83 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 84-85 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 86-87 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 88-89 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 90-91 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 92-93 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 94-95 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 96-97 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 98-99 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 100-101 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 102-103 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 104-105 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 106-107 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 108-109 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 110-111 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 112-113 0.0 5.073844736291994E-5 0.0 2.180738467658299 8.11815157806719E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCG 40 1.9444997E-9 2302.3083 110-111 CCGCTCC 400 0.0 2082.8818 108-109 CGTCGCT 45 3.1141099E-9 2046.4962 110-111 CCGCTCG 155 0.0 1897.122 108-109 CCGTTCG 70 0.0 1750.3208 108-109 CCGCGCC 345 0.0 1491.5778 108-109 TTGTTCG 235 0.0 1251.2931 108-109 CGTTGCG 50 1.1721195E-5 1227.8978 110-111 CGCGTTG 25 0.0054205107 1227.8978 110-111 CTCCGCC 250 0.0 1227.8977 110-111 CGTCGCG 40 1.1811062E-5 1225.2246 108-109 CCGCGTG 40 1.1811062E-5 1225.2246 108-109 CCGCGCG 165 0.0 1188.0967 108-109 CGCCGCT 90 4.9774826E-8 1023.2481 110-111 CCGTGCG 75 5.9240847E-8 980.17975 108-109 CCGCGCT 50 2.3061451E-5 980.1796 108-109 CCACGCG 55 3.069012E-5 891.07245 108-109 CGTCGCC 180 0.0 816.8164 108-109 CGTCGGT 80 4.7975034E-5 767.4361 110-111 GGCGTCG 100 2.7284841E-11 735.1347 108-109 >>END_MODULE