FastQCFastQC Report
Fri 27 May 2016
SRR939925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970892
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55220.2801777063380439No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45320.22994664344875315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42790.2171098162659344No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA28610.14516269790531394No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC25550.12963673301226045No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24050.12202596590782246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.11624178290844957No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA22900.11619104446108665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111400.034.8780981
TACATGG108200.034.252112
GTATCAA160750.034.1798671
ACATGGG104900.032.5464253
ATCAACG184150.029.5732233
TATCAAC185200.029.5020732
TCAACGC185650.029.3021854
CAACGCA187050.029.1147215
CATGGGG55850.029.0181624
AACGCAG189250.028.796076
GAGTACT86700.027.75703612-13
TAGGACC23000.027.7191644
AGTACTT89500.026.09097312-13
AGAGTAC147400.026.0740610-11
ACGCAGA208600.026.0108287
CGCAGAG208900.025.9165138
GGTATCA114000.025.8756181
GTACTTT92300.025.72008514-15
GTGTAGC14850.024.8802991
GCAGAGT222400.024.1828389
Produced by FastQC (version 0.11.5)