FastQCFastQC Report
Fri 27 May 2016
SRR939924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812006
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA31270.1725711725016363No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC27770.15325556317142439No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA24120.1331121420127748No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA18810.10380760328608184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC3400.02260.6033108-109
CCGCTCG750.01983.4972108-109
CTCCGCC2350.01923.6766110-111
CAGCGCG1050.01889.0448108-109
CCGCGCC3100.01759.554108-109
CGCCGCC1600.01318.5201110-111
CCGCGCT401.1397693E-51239.6857108-109
GGCCGTA300.008096251004.58673110-111
CGCTACG300.008096251004.58673110-111
CGACGCC502.2254417E-5991.74854108-109
TTGTTCG2900.0940.4512108-109
CGTTGCT1700.0886.4001110-111
CGTTGCG703.3958666E-5861.07434110-111
AAGCGCG1203.5470293E-10826.4571108-109
CATCGCG1203.5470293E-10826.4571108-109
CGCGTCT754.1762512E-5803.66943110-111
CGTCGCC1254.3655746E-10793.39886108-109
CCGCGTC654.887089E-5762.8835108-109
CGCCGCG654.887089E-5762.8835108-109
CGCGTTC401.079801E-4753.44006110-111
Produced by FastQC (version 0.11.5)