##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939924_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1812006 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.923132152984042 34.0 31.0 34.0 30.0 34.0 2 32.054830392393846 34.0 31.0 34.0 30.0 34.0 3 32.213201280790464 34.0 31.0 34.0 30.0 34.0 4 35.436243588597385 37.0 35.0 37.0 33.0 37.0 5 35.4810000629137 37.0 37.0 37.0 33.0 37.0 6 35.54914994762711 37.0 37.0 37.0 35.0 37.0 7 35.477969719747065 37.0 37.0 37.0 35.0 37.0 8 35.492395720543975 37.0 37.0 37.0 35.0 37.0 9 37.133016115840675 39.0 39.0 39.0 35.0 39.0 10-11 37.11315359882914 39.0 39.0 39.0 35.0 39.0 12-13 37.053415661979045 39.0 39.0 39.0 35.0 39.0 14-15 38.58771963227495 41.0 40.0 41.0 35.0 41.0 16-17 38.59695359728389 41.0 40.0 41.0 35.0 41.0 18-19 38.62577193453002 41.0 40.0 41.0 35.0 41.0 20-21 38.6857772546007 41.0 40.0 41.0 34.5 41.0 22-23 38.688171562345815 41.0 39.0 41.0 34.0 41.0 24-25 38.72831105415766 41.0 39.0 41.0 34.0 41.0 26-27 38.69868615225336 41.0 39.0 41.0 34.0 41.0 28-29 38.653094139864876 41.0 39.0 41.0 34.0 41.0 30-31 38.57237393253665 41.0 39.0 41.0 34.0 41.0 32-33 38.4787188894518 41.0 39.0 41.0 34.0 41.0 34-35 38.42962274959355 40.5 39.0 41.0 34.0 41.0 36-37 38.60186886798388 41.0 39.0 41.0 34.0 41.0 38-39 38.560395495379154 41.0 39.0 41.0 34.0 41.0 40-41 38.47888941868846 41.0 39.0 41.0 33.5 41.0 42-43 38.44151454244633 41.0 39.0 41.0 33.0 41.0 44-45 38.381912918610645 41.0 39.0 41.0 33.0 41.0 46-47 38.29449957671222 41.0 39.0 41.0 33.0 41.0 48-49 38.221679177662764 41.0 39.0 41.0 33.0 41.0 50-51 38.12225649363192 41.0 38.5 41.0 33.0 41.0 52-53 38.039629559725526 40.5 38.0 41.0 33.0 41.0 54-55 37.91832008282533 40.0 38.0 41.0 33.0 41.0 56-57 37.747409224914264 40.0 38.0 41.0 32.5 41.0 58-59 37.60594887654897 40.0 38.0 41.0 32.0 41.0 60-61 37.41529581027878 40.0 37.0 41.0 32.0 41.0 62-63 37.183584657004445 40.0 37.0 41.0 31.0 41.0 64-65 36.91112777772259 40.0 36.0 41.0 31.0 41.0 66-67 36.61764778924573 39.0 36.0 41.0 31.0 41.0 68-69 36.23046529647253 39.0 35.0 41.0 30.5 41.0 70-71 35.826886610750734 38.5 35.0 40.5 30.0 41.0 72-73 35.43362908290591 37.5 35.0 40.0 30.0 41.0 74-75 35.009507694786876 37.0 35.0 39.0 29.0 41.0 76-77 34.533083223786235 36.5 35.0 39.0 29.0 41.0 78-79 34.05222030169878 36.0 34.0 39.0 29.0 40.0 80-81 33.601953856664935 36.0 34.0 37.5 28.0 39.0 82-83 33.36226176955264 35.0 34.0 37.0 29.0 39.0 84-85 32.90105551526871 35.0 34.0 37.0 28.0 39.0 86-87 32.23589491425525 35.0 34.0 36.0 25.5 37.0 88-89 16.560164259941743 18.0 17.0 18.5 2.0 19.5 90-91 2.837857049038469 2.0 2.0 2.0 2.0 2.0 92-93 2.321242313767173 2.0 2.0 2.0 2.0 2.0 94-95 2.106915760764589 2.0 2.0 2.0 2.0 2.0 96-97 2.000042770277803 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.05216646322559271 1101 2 0.138538745857268 1101 3 0.1690422662613642 1101 4 0.1661554269286114 1101 5 0.19464728304900802 1101 6 0.10856360424672573 1101 7 0.1729477743018819 1101 8 0.21194791273435953 1101 9 0.10374399669347412 1101 10-11 0.17436426904288282 1101 12-13 0.21841545219947278 1101 14-15 0.28314702763333344 1101 16-17 0.32029326114835044 1101 18-19 0.30142695730789626 1101 20-21 0.25302409990906227 1101 22-23 0.2639920315969633 1101 24-25 0.25347788642817903 1101 26-27 0.27912439094858854 1101 28-29 0.34314433804237865 1101 30-31 0.25960874426440483 1101 32-33 0.3488441131174653 1101 34-35 0.3196435991159561 1101 36-37 0.28504816404830535 1101 38-39 0.2890601242663138 1101 40-41 0.27137214359512996 1101 42-43 0.23638976539740497 1101 44-45 0.22909812873734126 1101 46-47 0.1878976425117429 1101 48-49 0.17972699710543338 1101 50-51 0.1657822868260297 1101 52-53 0.06460369266267918 1101 54-55 0.15822405283361007 1101 56-57 0.14214326712745873 1101 58-59 0.0959472717288179 1101 60-61 0.09490069779901233 1101 62-63 0.15601016879306684 1101 64-65 0.13005324208249647 1101 66-67 0.16543759766408783 1101 68-69 0.2171504497323724 1101 70-71 0.18843433261321252 1101 72-73 0.29699583693880527 1101 74-75 0.2899230050665196 1101 76-77 0.17486087556884655 1101 78-79 0.05738038818790869 1101 80-81 0.12634719085131962 1101 82-83 0.2736961557542372 1101 84-85 0.2944001109926404 1101 86-87 0.5113152276749275 1101 88-89 0.709776530396681 1101 90-91 -0.09778225194418466 1101 92-93 -0.2215860708458055 1101 94-95 -0.02350897008014874 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.06647450393554877 1102 2 -0.0763856360296451 1102 3 0.10873592652938413 1102 4 -0.002710886680276303 1102 5 -0.03666043092584914 1102 6 -0.007542304239350983 1102 7 0.04588330968866927 1102 8 0.04827051526189763 1102 9 0.04750019630765934 1102 10-11 0.1619603013529911 1102 12-13 0.04867197069045659 1102 14-15 0.05528155588446282 1102 16-17 0.10250309550777814 1102 18-19 0.03463568882911261 1102 20-21 0.036835032272584556 1102 22-23 0.038839737714042144 1102 24-25 0.11761062132315914 1102 26-27 0.05726110149549868 1102 28-29 0.0865888804150785 1102 30-31 0.1601691048692757 1102 32-33 0.14849651608358272 1102 34-35 0.21068639179738113 1102 36-37 0.2181808769844622 1102 38-39 0.14439179641768618 1102 40-41 0.2118546625590909 1102 42-43 0.18394981684107847 1102 44-45 0.2661402854029191 1102 46-47 0.16071001894401604 1102 48-49 0.21299018333627373 1102 50-51 0.20299750741499167 1102 52-53 0.07928150387719057 1102 54-55 0.08251060056141313 1102 56-57 0.063187492661271 1102 58-59 0.10452993111863407 1102 60-61 0.03725801010916996 1102 62-63 0.1582497312849398 1102 64-65 0.1448753653864827 1102 66-67 0.1254532753124309 1102 68-69 0.06801950550792668 1102 70-71 0.03755023741683772 1102 72-73 0.03843912528921578 1102 74-75 0.10139114795554605 1102 76-77 -0.0042696057826674405 1102 78-79 0.1148309757126782 1102 80-81 0.12910610309045012 1102 82-83 0.12130409005977327 1102 84-85 0.0029289475812817045 1102 86-87 0.15770834960434854 1102 88-89 0.6822796666372497 1102 90-91 -0.008952118252896213 1102 92-93 -0.08801847852279776 1102 94-95 0.0502322959578847 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.31738656106814744 1103 2 -0.19355624089822143 1103 3 -0.15769575490218557 1103 4 -0.14473958478230742 1103 5 -0.26723360640919935 1103 6 -0.28377907436208005 1103 7 -0.3255100293560602 1103 8 -0.25369411638789074 1103 9 -0.2074609602169204 1103 10-11 -0.25797417144564605 1103 12-13 -0.315150982871387 1103 14-15 -0.2717335566433263 1103 16-17 -0.31896394911127857 1103 18-19 -0.29488509435107346 1103 20-21 -0.2861909596474419 1103 22-23 -0.21262731297881032 1103 24-25 -0.2004406998652044 1103 26-27 -0.17941563654541426 1103 28-29 -0.1847768532044114 1103 30-31 -0.21770171510295455 1103 32-33 -0.2338301960981255 1103 34-35 -0.27003536404129136 1103 36-37 -0.27652436297471183 1103 38-39 -0.26053902147123154 1103 40-41 -0.24626830214818085 1103 42-43 -0.21786050989601335 1103 44-45 -0.28465893569284617 1103 46-47 -0.2158494674067839 1103 48-49 -0.20694861704552636 1103 50-51 -0.24710697714556318 1103 52-53 -0.14618155511369224 1103 54-55 -0.23975449594672682 1103 56-57 -0.22873866449650393 1103 58-59 -0.24045429370189453 1103 60-61 -0.19603265983410978 1103 62-63 -0.17891166570142758 1103 64-65 -0.18266141071894992 1103 66-67 -0.26191613417138626 1103 68-69 -0.26131473606110944 1103 70-71 -0.22547358615625512 1103 72-73 -0.20259564084364 1103 74-75 -0.045422718289032105 1103 76-77 -0.12400927660088001 1103 78-79 -0.2253232866834196 1103 80-81 -0.3202802304322887 1103 82-83 -0.279783682133818 1103 84-85 -0.30798990917146796 1103 86-87 -0.2414439299994413 1103 88-89 0.6314002713691664 1103 90-91 -0.5992524555614311 1103 92-93 -0.3529335318613991 1103 94-95 -0.11887082533804572 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.20815315183953587 1104 2 -0.07948519768440931 1104 3 -0.030144750803259512 1104 4 0.0824794077332669 1104 5 0.028253651465725227 1104 6 0.11296910102721824 1104 7 0.08822914757487865 1104 8 -0.014926932790565672 1104 9 0.06658154598378019 1104 10-11 0.10359174629394374 1104 12-13 0.09933053418180293 1104 14-15 0.09074927973816216 1104 16-17 0.09076994760042822 1104 18-19 0.06547468415288193 1104 20-21 0.03501361034307138 1104 22-23 -0.06040501487746042 1104 24-25 0.0616350772380585 1104 26-27 0.10949386886630208 1104 28-29 0.07484908525502476 1104 30-31 0.0320955907356506 1104 32-33 0.07293375892265885 1104 34-35 0.02845292982601677 1104 36-37 0.0042325507851046495 1104 38-39 0.021745726773900742 1104 40-41 0.06756648249472619 1104 42-43 0.03548902928353925 1104 44-45 0.020636031534628785 1104 46-47 0.06464735850435943 1104 48-49 0.01999459517017499 1104 50-51 0.011461028561846831 1104 52-53 -0.02934043101110717 1104 54-55 0.08222118365991804 1104 56-57 0.13242004866437895 1104 58-59 0.03960285297457489 1104 60-61 0.07267404970588842 1104 62-63 0.05620222807521458 1104 64-65 0.009248331532539567 1104 66-67 0.02605705498513089 1104 68-69 0.04400791090669287 1104 70-71 -0.03170203627968249 1104 72-73 -0.02703205662966468 1104 74-75 -0.10419261900522514 1104 76-77 -0.17505231605431248 1104 78-79 -0.15084052004615245 1104 80-81 -0.20718117474006448 1104 82-83 -0.1403608301470527 1104 84-85 -0.0645128833559312 1104 86-87 -0.11019677243996284 1104 88-89 0.497393203954104 1104 90-91 -0.6502663622698086 1104 92-93 -0.3529335318613991 1104 94-95 -0.11887082533804572 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.09470306534300121 1105 2 -0.1548362060770927 1105 3 0.010637884867101377 1105 4 0.09955255249445827 1105 5 0.07471673480266361 1105 6 0.1339988676179047 1105 7 0.11968421303468801 1105 8 -0.00354005655407974 1105 9 0.06968221772500272 1105 10-11 0.13529930922132394 1105 12-13 0.1683395756781323 1105 14-15 0.09815696057341938 1105 16-17 0.13639340764626695 1105 18-19 0.09750544667819838 1105 20-21 0.13610991928015181 1105 22-23 -0.08048654948281353 1105 24-25 -0.0020055003898349355 1105 26-27 0.0041734625704066275 1105 28-29 -0.0629187718244495 1105 30-31 -0.09569780003487693 1105 32-33 -0.10220010306763072 1105 34-35 0.02104737857300165 1105 36-37 -0.10590262799687622 1105 38-39 -0.06795823999894424 1105 40-41 -0.003486744993637103 1105 42-43 -0.007927828631451916 1105 44-45 -0.028292043244938725 1105 46-47 -0.02302392202054193 1105 48-49 -0.07512795163981423 1105 50-51 -0.0708924011658496 1105 52-53 -0.10599055555617554 1105 54-55 -0.2270204224142205 1105 56-57 -0.2540715852717952 1105 58-59 -0.2540705131211922 1105 60-61 -0.30458321873576466 1105 62-63 -0.34642730908848307 1105 64-65 -0.22113718674033578 1105 66-67 -0.1427417967441258 1105 68-69 -0.147600683235396 1105 70-71 -0.21291500373185812 1105 72-73 -0.19680739280197201 1105 74-75 -0.24940868996858256 1105 76-77 -0.21836028133076724 1105 78-79 -0.28937133545609584 1105 80-81 -0.384502543607141 1105 82-83 -0.2541684025155817 1105 84-85 -0.269833076693601 1105 86-87 -0.2516913984391813 1105 88-89 0.5068435090877443 1105 90-91 -0.85347997285582 1105 92-93 -0.3529335318613991 1105 94-95 -0.11887082533804572 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.015271258207967264 1106 2 0.03904939030797294 1106 3 0.1358099503316481 1106 4 0.03877862857046921 1106 5 0.1664563454019259 1106 6 0.17816277564827487 1106 7 -0.03795546587806342 1106 8 0.1248026970199092 1106 9 0.09554240648085255 1106 10-11 0.03858396861691915 1106 12-13 0.08277907944229668 1106 14-15 0.17845392332524312 1106 16-17 0.03185631120508248 1106 18-19 0.051870027312361344 1106 20-21 0.0960994159081281 1106 22-23 0.035925177157920984 1106 24-25 0.017623888543944588 1106 26-27 -0.039787306365113295 1106 28-29 0.12104778920804193 1106 30-31 0.17366186840221332 1106 32-33 0.21223683720864273 1106 34-35 0.13823431781003137 1106 36-37 0.21778628651149745 1106 38-39 0.16757786846015676 1106 40-41 0.2192688520704067 1106 42-43 -0.16042612079424146 1106 44-45 0.16652153575829232 1106 46-47 0.006468590516298889 1106 48-49 0.08148009660364153 1106 50-51 0.06528499496786111 1106 52-53 0.09640263360290646 1106 54-55 0.09888424632078596 1106 56-57 -0.020199249740329606 1106 58-59 0.12538818908880955 1106 60-61 0.14555541746096168 1106 62-63 0.17484388991716315 1106 64-65 0.16756974839510974 1106 66-67 0.1596827778208052 1106 68-69 0.14438737783935807 1106 70-71 0.21622624167984839 1106 72-73 0.16005769706561068 1106 74-75 0.018743506386279307 1106 76-77 0.06806182397177452 1106 78-79 0.0140120528652119 1106 80-81 -0.10442271520840052 1106 82-83 -0.08176143980532657 1106 84-85 -0.08946980967377272 1106 86-87 -0.07562342859688442 1106 88-89 0.5524424160719335 1106 90-91 -0.29794491750526664 1106 92-93 -0.34260142854036557 1106 94-95 -0.11887082533804572 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.039157792812261505 1107 2 0.029996786140607412 1107 3 0.10422395931713879 1107 4 0.09990880807887947 1107 5 0.10917285150478051 1107 6 0.11830914219014943 1107 7 0.031067822026699332 1107 8 -0.008063464308129653 1107 9 0.06089740306464364 1107 10-11 -0.0062568255531090244 1107 12-13 0.08850219378512492 1107 14-15 0.06895493073619008 1107 16-17 0.0633965291172629 1107 18-19 0.026045876564488424 1107 20-21 0.004554553569718678 1107 22-23 0.09390117379675189 1107 24-25 0.10596664719887627 1107 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-0.24840436844262115 2216 7 -0.230542865916199 2216 8 -0.26292596750078445 2216 9 -0.3586831904863672 2216 10-11 -0.34977671286223 2216 12-13 -0.35242824679509255 2216 14-15 -0.35523701084854054 2216 16-17 -0.43159662119658293 2216 18-19 -0.4579775483670403 2216 20-21 -0.30112184049896484 2216 22-23 -0.45486206810054597 2216 24-25 -0.4805176339047179 2216 26-27 -0.4992787766597502 2216 28-29 -0.4597404654830157 2216 30-31 -0.4678774041333682 2216 32-33 -0.5902889874417028 2216 34-35 -0.543736094599268 2216 36-37 -0.5903138492011095 2216 38-39 -0.541453749211648 2216 40-41 -0.6210439598723028 2216 42-43 -0.5577533676229436 2216 44-45 -0.6068221536056555 2216 46-47 -0.5413431839540266 2216 48-49 -0.5026213401554003 2216 50-51 -0.5870467943074829 2216 52-53 -0.5246872171132253 2216 54-55 -0.5204503132862115 2216 56-57 -0.4926484696732629 2216 58-59 -0.5629410878209882 2216 60-61 -0.5651305480613118 2216 62-63 -0.597598600465183 2216 64-65 -0.6414394374414343 2216 66-67 -0.7012701265410612 2216 68-69 -0.8464957635148522 2216 70-71 -0.7856827075008397 2216 72-73 -0.6930664967903226 2216 74-75 -0.6971376721823432 2216 76-77 -0.7535355470171297 2216 78-79 -0.6745297656836726 2216 80-81 -0.6815714046284285 2216 82-83 -0.6866605526262433 2216 84-85 -0.7215316318117573 2216 86-87 -0.6937601282009638 2216 88-89 -1.2852195137388538 2216 90-91 -0.85347997285582 2216 92-93 -0.3529335318613991 2216 94-95 -0.11887082533804572 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15852.0 3 2835.0 4 1859.0 5 1556.0 6 1659.0 7 1949.0 8 2172.0 9 2662.0 10 3303.0 11 4075.0 12 5071.0 13 6115.0 14 7095.0 15 7944.0 16 8679.0 17 10155.0 18 13035.0 19 16039.0 20 16513.0 21 23687.0 22 27285.0 23 33690.0 24 51513.0 25 86160.0 26 154770.0 27 461384.0 28 811789.0 29 31913.0 30 1247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.16614735271296 21.155779837373608 15.338746118942211 23.33932669097122 2 12.591970807753693 21.091751159499953 45.17391947408041 21.142358558665954 3 17.630621891220247 27.570324883875397 30.20034772507303 24.59870549983133 4 11.105123317865685 18.52109114280224 41.08245191298951 29.29133362634256 5 11.80973321104747 39.72271441484172 35.95044220032831 12.517110173782491 6 27.92170666101547 38.731273516754364 20.807215870146127 12.539803952084045 7 26.3501334984542 32.74249643764976 24.16101271187844 16.7463573520176 8 23.19186581059886 39.2870663783674 21.982046417064844 15.539021393968893 9 25.433028367455734 18.05529341514322 22.860299579581966 33.65137863781908 10-11 22.91469427749367 27.503753460620327 31.12721920513556 18.45433305675045 12-13 25.233722484489324 25.333447757091974 30.089714113526767 19.343115644891938 14-15 20.051947951607225 29.902632772739164 26.93581036707384 23.109608908579773 16-17 19.503841607238844 30.77587662706774 30.342295427929994 19.377986337763424 18-19 20.615838965586136 28.916388779426523 31.123343300847843 19.3444289541395 20-21 21.79182087199734 28.880262343141034 29.07565654770643 20.2522602371552 22-23 20.45702011976828 30.814807292940007 29.255479721567173 19.47269286572454 24-25 20.933484767710482 29.23569237629456 30.563806080112315 19.267016775882638 26-27 19.394913703376258 30.567724389433586 29.93157859300687 20.105783314183288 28-29 19.626928993715463 30.495905738864092 28.64705192502708 21.230113342393366 30-31 20.484396850783053 29.1703228355756 29.7538473934413 20.59143292020004 32-33 19.504294466338813 30.392310523884607 30.2886671674072 19.814727842369383 34-35 19.86790327071303 30.48922864748479 30.218202980068217 19.42466510173396 36-37 19.371983315728535 29.803874821606552 30.006192032476715 20.8179498301882 38-39 20.58563823740098 30.4903791709299 29.004815657343297 19.919166934325823 40-41 21.836875816084493 28.649436039395017 30.215297300340065 19.298390844180428 42-43 19.597396476612108 30.655472443247984 30.197609720939113 19.549521359200796 44-45 19.336867172000076 30.640367990154555 29.563632550682804 20.45913228716256 46-47 20.845764307623703 29.152138569077586 29.37219854680393 20.629898576494778 48-49 20.133283333457506 29.593414365140923 29.368111392659348 20.905190908742224 50-51 19.433931234223287 30.83212748743658 29.448357235020197 20.285584043319947 52-53 19.475928887652692 30.159999470200425 30.05059034020859 20.313481301938292 54-55 19.69504562974344 29.341144695073222 30.35661068358521 20.60719899159812 56-57 20.220269690056213 29.709366304526586 28.53472891370117 21.53563509171603 58-59 20.60718893866797 30.019657771552634 28.15892993731809 21.214223352461307 60-61 19.86927747479865 28.83558332588303 30.497112040467854 20.79802715885047 62-63 20.31370765706837 28.84712546402315 30.270355139650512 20.568811739257967 64-65 20.631731148644732 29.67861567710906 28.89368406820069 20.795969106045515 66-67 20.61041740479888 30.61319885254243 28.229569880011436 20.546813862647255 68-69 21.211916348693542 28.758061543114287 30.06449234491429 19.965529763277885 70-71 21.806522715708446 28.78652167818429 29.317480184944202 20.089475421163066 72-73 20.863010387382825 29.894216685816716 28.76458466473069 20.47818826206977 74-75 20.097891508085514 30.412012984504464 29.903736521843747 19.586358985566275 76-77 20.572034529686988 29.0335683215177 30.20569468313019 20.188702465665127 78-79 20.246528765598445 29.01802677097869 29.763529184196795 20.97191527922607 80-81 20.68431340735075 29.823990649037587 29.407408143240144 20.084287800371524 82-83 20.240519228375994 29.778368777202385 30.243408306804128 19.737703687617497 84-85 20.245120804059813 29.421450112292767 30.233396219490626 20.100032864156795 86-87 19.306015034896824 30.93820614911441 28.718485174680026 21.03729364130874 88-89 29.232005320390513 18.61993143777334 35.84367046797086 16.30439277386529 90-91 31.097917986218178 16.20694209112734 18.07286430938805 34.62227561326643 92-93 22.639479822418057 18.180200944097173 36.913127534442545 22.267191699042225 94-95 16.3184258744837 7.31699653787343 29.236067957824098 47.12850962981878 96-97 27.826271474072367 7.469323899110544 16.54534482786267 48.15905979895442 98-99 57.22529578337417 14.186084380175465 18.84951288578929 9.73910695066107 100-101 31.083500016563608 7.183523662545564 13.348613740028991 48.38436258086183 102-103 23.372896416387974 2.1216225196134526 38.3559807138056 36.14950035019297 104-105 40.52454897076751 4.435084241823588 43.63987387845672 11.400492908952174 106-107 6.344322124716162 2.050974602627418 7.623258567585189 83.98144470507123 108-109 18.926379680530427 12.148203524286652 23.44348005075195 45.481936744430975 110-111 40.8216008028867 2.8968836534060896 26.694217233001467 29.587298310705744 112-113 27.371718145664314 2.7772398722281184 40.526544729065115 29.324497253042452 114-115 29.0583697222199 3.970284960466223 25.676290587871854 41.29505472944202 116-117 20.501521842946467 4.3544517296733485 21.942731360814456 53.201295066565734 118-119 21.274769135371248 4.383876955922811 17.42718884785501 56.91416506085093 120-121 26.000190967249114 4.282300144348774 31.60690515106071 38.1106037373414 122-123 34.61616574209934 1.6901212396428302 35.497566307564874 28.19614671069296 124-125 39.21567870117373 2.147605531841637 20.96395607015004 37.672759696834596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 16.0 2 41.5 3 59.5 4 74.5 5 84.5 6 83.5 7 83.5 8 83.5 9 88.0 10 91.0 11 94.0 12 100.5 13 114.5 14 137.0 15 191.5 16 272.5 17 397.5 18 596.0 19 877.5 20 1361.5 21 2187.5 22 3328.0 23 4914.0 24 7135.5 25 10054.0 26 13899.5 27 18520.5 28 24394.5 29 31583.0 30 40178.0 31 50269.0 32 62266.0 33 75487.0 34 86997.5 35 95328.0 36 99156.0 37 98267.5 38 94434.0 39 89761.0 40 85039.0 41 79596.5 42 73919.5 43 68753.0 44 64421.0 45 60353.5 46 56441.0 47 52663.5 48 48760.5 49 45251.5 50 41616.5 51 37564.5 52 33215.0 53 28502.0 54 24044.5 55 20299.5 56 16932.0 57 13821.5 58 11110.0 59 8899.5 60 6990.5 61 5360.0 62 4069.0 63 3035.5 64 2240.0 65 1675.5 66 1217.0 67 870.5 68 637.5 69 454.5 70 327.5 71 228.0 72 173.0 73 136.0 74 100.0 75 68.0 76 45.5 77 34.0 78 20.5 79 13.0 80 7.0 81 6.5 82 8.0 83 6.5 84 4.0 85 3.5 86 3.0 87 2.5 88 4.0 89 5.5 90 7.0 91 7.0 92 3.0 93 2.0 94 3.5 95 2.5 96 3.0 97 6.0 98 7.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 9.93374194125185E-4 3 0.044425901459487445 4 0.05044133407946773 5 0.05237289501248892 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.346557351355348E-4 12-13 0.0015452487464169544 14-15 0.0 16-17 2.2074982091670778E-4 18-19 0.006622494627501234 20-21 0.0015452487464169544 22-23 3.863121866042386E-4 24-25 0.0 26-27 0.0 28-29 0.0010209679217397735 30-31 0.0 32-33 0.0012693114702710697 34-35 2.7593727614588472E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.5187455229176944E-5 46-47 0.0 48-49 8.278118284376542E-5 50-51 0.0 52-53 0.0 54-55 0.0016556236568753084 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.7593727614588472E-5 64-65 5.5187455229176944E-5 66-67 0.0 68-69 4.4149964183341555E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.5871845898965016E-4 80-81 0.0 82-83 5.24280824677181E-4 84-85 8.278118284376542E-5 86-87 0.0011865302874273043 88-89 19.257966033225056 90-91 26.243594116134272 92-93 40.28209619614946 94-95 44.67109932307067 96-97 48.37437072504175 98-99 93.05220291765039 100-101 51.68818425546052 102-103 53.077114534940826 104-105 74.83076766853974 106-107 81.58960290418464 108-109 12.595129375951295 110-111 39.04465548127324 112-113 51.97893936333544 114-115 86.7802865994925 116-117 66.42900189072222 118-119 71.87614169048005 120-121 80.34874056708422 122-123 75.8155878071044 124-125 49.99067332006627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1812006.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.77947830122076 #Duplication Level Percentage of deduplicated Percentage of total 1 84.94608340042655 62.67277717015459 2 10.333092228712848 15.247403077456651 3 2.481359325496605 5.492201895390257 4 0.8676007242698611 2.5604451524156655 5 0.4220534960877459 1.5569443378280106 6 0.23530612210285423 1.0416457757899165 7 0.1525054803805128 0.7876242346385904 8 0.09957958142754379 0.5877543653742491 9 0.06881179780159305 0.45692086884935634 >10 0.35293904553959976 4.5850983940013395 >50 0.023626329575415817 1.1936329989184373 >100 0.014725927392544857 2.055878005737473 >500 0.0016439966870661195 0.8444213297121438 >1k 6.72544099254321E-4 0.9172523937334112 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3127 0.1725711725016363 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2777 0.15325556317142439 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 2412 0.1331121420127748 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1881 0.10380760328608184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.5187455229176944E-5 0.0 5 0.0 0.0 0.0 5.5187455229176944E-5 0.0 6 0.0 0.0 0.0 5.5187455229176944E-5 5.5187455229176944E-5 7 0.0 0.0 0.0 1.1037491045835389E-4 5.5187455229176944E-5 8 0.0 0.0 0.0 1.1037491045835389E-4 5.5187455229176944E-5 9 0.0 0.0 0.0 1.1037491045835389E-4 5.5187455229176944E-5 10-11 0.0 0.0 0.0 1.1037491045835389E-4 5.5187455229176944E-5 12-13 0.0 0.0 0.0 2.2074982091670778E-4 5.5187455229176944E-5 14-15 0.0 0.0 0.0 2.2074982091670778E-4 5.5187455229176944E-5 16-17 0.0 0.0 0.0 2.759372761458847E-4 5.5187455229176944E-5 18-19 0.0 0.0 0.0 2.759372761458847E-4 5.5187455229176944E-5 20-21 0.0 0.0 0.0 3.863121866042386E-4 5.5187455229176944E-5 22-23 0.0 0.0 0.0 4.1390591421882705E-4 5.5187455229176944E-5 24-25 0.0 0.0 0.0 4.6909336944800405E-4 5.5187455229176944E-5 26-27 0.0 0.0 0.0 7.450306455938887E-4 5.5187455229176944E-5 28-29 0.0 0.0 0.0 0.0012969051978856583 5.5187455229176944E-5 30-31 0.0 0.0 0.0 0.0032284661309068515 5.5187455229176944E-5 32-33 0.0 0.0 0.0 0.006843244448417942 5.5187455229176944E-5 34-35 0.0 0.0 0.0 0.013107020616929525 5.5187455229176944E-5 36-37 0.0 0.0 0.0 0.023482262200014793 5.5187455229176944E-5 38-39 0.0 0.0 0.0 0.04254952798169542 5.5187455229176944E-5 40-41 0.0 0.0 0.0 0.06652847727877281 5.5187455229176944E-5 42-43 0.0 0.0 0.0 0.089514052381725 5.5187455229176944E-5 44-45 0.0 0.0 0.0 0.11688703017539677 5.5187455229176944E-5 46-47 0.0 0.0 0.0 0.15179309560785118 5.5187455229176944E-5 48-49 0.0 0.0 0.0 0.19067265781680637 5.5187455229176944E-5 50-51 0.0 0.0 0.0 0.23327737325373094 5.5187455229176944E-5 52-53 0.0 0.0 0.0 0.27494390195175955 5.5187455229176944E-5 54-55 0.0 0.0 0.0 0.3223775197212371 5.5187455229176944E-5 56-57 0.0 0.0 0.0 0.37811684950270585 5.5187455229176944E-5 58-59 0.0 0.0 0.0 0.4430724843074471 5.5187455229176944E-5 60-61 0.0 0.0 0.0 0.5215214519157221 5.5187455229176944E-5 62-63 0.0 0.0 0.0 0.5999980132516117 1.1037491045835389E-4 64-65 0.0 0.0 0.0 0.6779502937628241 1.1037491045835389E-4 66-67 0.0 0.0 0.0 0.7687060638872056 1.1037491045835389E-4 68-69 0.0 0.0 0.0 0.8817023784689455 1.1037491045835389E-4 70-71 0.0 0.0 0.0 1.010868617432834 1.1037491045835389E-4 72-73 0.0 0.0 0.0 1.1366408279001283 1.6556236568753083E-4 74-75 0.0 0.0 0.0 1.2614196641732973 1.6556236568753083E-4 76-77 0.0 0.0 0.0 1.402120081280084 1.6556236568753083E-4 78-79 0.0 0.0 0.0 1.4404477689367474 1.6556236568753083E-4 80-81 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 82-83 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 84-85 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 86-87 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 88-89 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 90-91 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 92-93 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 94-95 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 96-97 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 98-99 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 100-101 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 102-103 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 104-105 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 106-107 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 108-109 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 110-111 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 112-113 0.0 0.0 0.0 1.441551518041331 1.6556236568753083E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 340 0.0 2260.6033 108-109 CCGCTCG 75 0.0 1983.4972 108-109 CTCCGCC 235 0.0 1923.6766 110-111 CAGCGCG 105 0.0 1889.0448 108-109 CCGCGCC 310 0.0 1759.554 108-109 CGCCGCC 160 0.0 1318.5201 110-111 CCGCGCT 40 1.1397693E-5 1239.6857 108-109 GGCCGTA 30 0.00809625 1004.58673 110-111 CGCTACG 30 0.00809625 1004.58673 110-111 CGACGCC 50 2.2254417E-5 991.74854 108-109 TTGTTCG 290 0.0 940.4512 108-109 CGTTGCT 170 0.0 886.4001 110-111 CGTTGCG 70 3.3958666E-5 861.07434 110-111 AAGCGCG 120 3.5470293E-10 826.4571 108-109 CATCGCG 120 3.5470293E-10 826.4571 108-109 CGCGTCT 75 4.1762512E-5 803.66943 110-111 CGTCGCC 125 4.3655746E-10 793.39886 108-109 CCGCGTC 65 4.887089E-5 762.8835 108-109 CGCCGCG 65 4.887089E-5 762.8835 108-109 CGCGTTC 40 1.079801E-4 753.44006 110-111 >>END_MODULE