FastQCFastQC Report
Fri 27 May 2016
SRR939924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812006
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.2248336926036669No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA31510.17389567142713655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.15667718539563333No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC27160.1498891284024446No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA21610.11926009075025137No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA21260.11732852981723017No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20350.11230647139137508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.10380760328608184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95900.038.3310131
GTACATG81400.034.9122431
TACATGG80900.033.5032272
ACATGGG77550.032.5692523
ATCAACG111900.032.473083
TCAACGC113350.032.110234
CAACGCA115100.031.6220235
TATCAAC116050.031.465822
AACGCAG116600.031.2274826
GGTATCA66100.030.194451
TAGGACC30550.029.0535224
ACGCAGA129800.027.8684647
CATGGGG39600.027.829174
CGCAGAG130900.027.5433738
AGAGTAC106100.026.38325510-11
GACGGAC1159.982164E-425.899773
CGCGATC701.0570777E-425.49807222-23
AGTACTT68250.025.32372712-13
GCAGAGT144050.024.9050929
GAGTACT64700.024.50626412-13
Produced by FastQC (version 0.11.5)