##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939924_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1812006 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86852361416022 34.0 31.0 34.0 31.0 34.0 2 32.964478594441744 34.0 33.0 34.0 31.0 34.0 3 33.00259215477212 34.0 33.0 34.0 31.0 34.0 4 36.38914385493205 37.0 37.0 37.0 35.0 37.0 5 36.294583461644166 37.0 37.0 37.0 35.0 37.0 6 36.425066473289824 37.0 37.0 37.0 35.0 37.0 7 36.42097653098279 37.0 37.0 37.0 35.0 37.0 8 36.45258900908717 37.0 37.0 37.0 35.0 37.0 9 38.29972251747511 39.0 39.0 39.0 37.0 39.0 10-11 38.29499543599745 39.0 39.0 39.0 37.0 39.0 12-13 38.28046871809475 39.0 39.0 39.0 37.0 39.0 14-15 39.829553544524686 41.0 40.0 41.0 38.0 41.0 16-17 39.85462079043889 41.0 40.0 41.0 38.0 41.0 18-19 39.89824343848751 41.0 40.0 41.0 38.0 41.0 20-21 39.91082617827976 41.0 40.0 41.0 38.0 41.0 22-23 39.85961001232888 41.0 40.0 41.0 38.0 41.0 24-25 39.83245419717153 41.0 40.0 41.0 38.0 41.0 26-27 39.74733830903429 41.0 40.0 41.0 37.0 41.0 28-29 39.667771795457625 41.0 40.0 41.0 37.0 41.0 30-31 39.54372060578166 41.0 40.0 41.0 37.0 41.0 32-33 39.40248790566919 41.0 40.0 41.0 36.0 41.0 34-35 39.31242225467244 41.0 39.0 41.0 35.0 41.0 36-37 39.29590051026321 41.0 39.5 41.0 35.5 41.0 38-39 39.33900467216996 41.0 40.0 41.0 36.0 41.0 40-41 39.248218548945204 41.0 40.0 41.0 35.0 41.0 42-43 39.16398400446798 41.0 40.0 41.0 35.0 41.0 44-45 39.02007995558513 41.0 40.0 41.0 35.0 41.0 46-47 38.7940371058374 41.0 40.0 41.0 35.0 41.0 48-49 38.56857482811867 41.0 39.5 41.0 35.0 41.0 50-51 38.43040420395959 41.0 39.0 41.0 35.0 41.0 52-53 38.34122182818379 41.0 39.0 41.0 35.0 41.0 54-55 38.25793761168561 41.0 39.0 41.0 35.0 41.0 56-57 38.1296265023405 41.0 39.0 41.0 34.5 41.0 58-59 37.997541398869544 41.0 38.5 41.0 34.0 41.0 60-61 37.842872484969696 40.5 38.0 41.0 34.0 41.0 62-63 37.65872712342012 40.0 37.5 41.0 34.0 41.0 64-65 37.44182166063467 40.0 37.0 41.0 34.0 41.0 66-67 37.19425515147301 39.5 37.0 41.0 33.5 41.0 68-69 36.87761878271927 39.0 36.0 41.0 33.0 41.0 70-71 36.52517430957734 39.0 35.5 41.0 32.5 41.0 72-73 36.18570081997521 38.5 35.0 40.5 32.0 41.0 74-75 35.825270170187075 37.0 35.0 39.5 32.0 41.0 76-77 35.423604557600804 37.0 35.0 39.0 32.0 41.0 78-79 35.03106032761481 36.0 35.0 39.0 31.0 40.5 80-81 34.71310553055564 36.0 35.0 38.0 31.5 39.0 82-83 34.433954412954485 35.5 35.0 37.0 32.0 39.0 84-85 34.11664310162328 35.0 35.0 37.0 31.0 39.0 86-87 33.86330812370379 35.0 35.0 36.5 31.0 37.0 88-89 33.617932832451984 35.0 35.0 36.0 31.0 37.0 90-91 33.415204199103094 35.0 35.0 36.0 31.0 37.0 92-93 33.2656210851399 35.0 35.0 35.5 31.0 36.0 94-95 33.15333006623598 35.0 35.0 35.0 31.0 36.0 96-97 33.0327910062108 35.0 34.5 35.0 31.0 36.0 98-99 32.92646437153078 35.0 34.0 35.0 30.5 36.0 100-101 32.90603149216945 35.0 34.0 35.0 31.0 36.0 102-103 32.10143702614671 34.5 33.0 35.0 28.0 35.0 104-105 32.74478809672816 35.0 34.0 35.0 30.0 35.0 106-107 32.809961721981054 35.0 34.0 35.0 31.0 35.0 108-109 32.80679616954911 35.0 34.0 35.0 31.0 35.0 110-111 32.79203987183266 35.0 34.0 35.0 31.0 35.0 112-113 32.72517116389239 35.0 34.0 35.0 30.0 35.0 114-115 32.67170721289003 35.0 34.0 35.0 30.0 35.0 116-117 32.61888454011742 35.0 34.0 35.0 30.0 35.0 118-119 32.544132856072224 35.0 34.0 35.0 30.0 35.0 120-121 32.45870267537745 35.0 34.0 35.0 30.0 35.0 122-123 32.30867392271328 35.0 34.0 35.0 29.0 35.0 124-125 30.3346432075832 33.5 30.5 34.5 23.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07812360514655126 1101 2 0.06733508129922683 1101 3 0.07459048080684738 1101 4 0.010982809187375153 1101 5 0.020846698456651325 1101 6 0.07822484050786471 1101 7 0.10080042846454518 1101 8 0.14037891607574693 1101 9 0.08222047150082545 1101 10-11 0.1146178902453272 1101 12-13 0.0902159661879196 1101 14-15 0.2195481734865794 1101 16-17 0.205783647327884 1101 18-19 0.21531836483870848 1101 20-21 0.18966426910851908 1101 22-23 0.21291027905065363 1101 24-25 0.24296320450914521 1101 26-27 0.18061760918948977 1101 28-29 0.22376516868546048 1101 30-31 0.24846360348463037 1101 32-33 0.19749418879386837 1101 34-35 0.29711914812460094 1101 36-37 0.2952691461752508 1101 38-39 0.19275146261035303 1101 40-41 0.3096321271647895 1101 42-43 0.28717023171473954 1101 44-45 0.2709974746749708 1101 46-47 0.26126797149687064 1101 48-49 0.2641064339310546 1101 50-51 0.3268351601030446 1101 52-53 0.21818793598260555 1101 54-55 0.2638843192811393 1101 56-57 0.324061435842637 1101 58-59 0.2664094356405542 1101 60-61 0.20202929490536548 1101 62-63 0.20909365478340192 1101 64-65 0.22677318888930387 1101 66-67 0.22546517052172277 1101 68-69 0.21720324125668355 1101 70-71 0.3275382722153424 1101 72-73 0.2938902780781234 1101 74-75 0.22666955046826587 1101 76-77 0.28250557637964135 1101 78-79 0.3211834530507005 1101 80-81 0.27305389879649766 1101 82-83 0.24853298654912948 1101 84-85 0.2408779690481424 1101 86-87 0.20803033961070128 1101 88-89 0.23313054760415497 1101 90-91 0.2509231074104079 1101 92-93 0.2885648276631301 1101 94-95 0.28448856012366974 1101 96-97 0.3011239338982037 1101 98-99 0.271381409722423 1101 100-101 0.27270083680652846 1101 102-103 0.3609141649670846 1101 104-105 0.25262823178392324 1101 106-107 0.3237253948853649 1101 108-109 0.30843420331379434 1101 110-111 0.3262176817866873 1101 112-113 0.1996112685638849 1101 114-115 0.13981426078426296 1101 116-117 0.20328900514353165 1101 118-119 0.18998737502668916 1101 120-121 0.21922718403912 1101 122-123 0.16263351184766606 1101 124-125 0.2956093294077604 1102 1 0.05343904988923498 1102 2 0.11351621374549836 1102 3 0.09732970593860557 1102 4 -0.01589162476089001 1102 5 -0.12831813185776753 1102 6 -0.006400179495990699 1102 7 0.017095309168922768 1102 8 0.0033102350534903735 1102 9 -0.009670231720058098 1102 10-11 0.0996636980778618 1102 12-13 0.08734852367460633 1102 14-15 0.0954441209155874 1102 16-17 0.19294634842682257 1102 18-19 0.04911586124907075 1102 20-21 0.1468706398198094 1102 22-23 0.16175381551990853 1102 24-25 0.11533815812311587 1102 26-27 0.12249877849182411 1102 28-29 0.14653434627717132 1102 30-31 0.0864784568084147 1102 32-33 0.07315016899364934 1102 34-35 0.026283665644335485 1102 36-37 0.010851581711513347 1102 38-39 -0.010017291602700595 1102 40-41 -0.051872974468992084 1102 42-43 0.006193250717331011 1102 44-45 -0.06618491827065043 1102 46-47 -0.0665564104058376 1102 48-49 -0.17763050227036814 1102 50-51 -0.1865837315263903 1102 52-53 -0.14679487373823008 1102 54-55 -0.21563205275558062 1102 56-57 -0.2430996886447474 1102 58-59 -0.18986913550703832 1102 60-61 -0.19955807488792487 1102 62-63 -0.19457830195740655 1102 64-65 -0.20500163180272324 1102 66-67 -0.22924089647857926 1102 68-69 -0.2026319854586376 1102 70-71 -0.17085596945583603 1102 72-73 -0.0910462253923967 1102 74-75 -0.19852820922675107 1102 76-77 -0.28734574030427495 1102 78-79 -0.19315549726407966 1102 80-81 -0.1664325744924966 1102 82-83 -0.16085853143869855 1102 84-85 -0.21490700262103246 1102 86-87 -0.16428229203360445 1102 88-89 -0.17827043921565888 1102 90-91 -0.14542858502206713 1102 92-93 -0.1627237995173587 1102 94-95 -0.14749834483777136 1102 96-97 -0.15904679828303614 1102 98-99 -0.07519058968800607 1102 100-101 -0.06393053198139853 1102 102-103 -0.0955577701624577 1102 104-105 -0.1545879414070228 1102 106-107 -0.22139057690055353 1102 108-109 -0.1693226986666332 1102 110-111 -0.2148794313238227 1102 112-113 -0.1909193623287564 1102 114-115 -0.1579235386040807 1102 116-117 -0.11869922840541136 1102 118-119 -0.14301115215272375 1102 120-121 -0.14479409800514986 1102 122-123 -0.09087275060191757 1102 124-125 -0.027345408096678625 1103 1 0.01168576829051915 1103 2 0.032840193627855285 1103 3 0.049133791845228814 1103 4 0.018490643870570977 1103 5 -0.06028518391722315 1103 6 0.021681431737626156 1103 7 -0.04231542281216605 1103 8 -0.053830717518934534 1103 9 7.560549615277523E-4 1103 10-11 0.03061513469881305 1103 12-13 0.01723355269675153 1103 14-15 -0.015813638744717196 1103 16-17 0.04832641500176038 1103 18-19 0.010161918577850315 1103 20-21 0.015703492158721133 1103 22-23 -0.0024391691792757797 1103 24-25 0.019873040113722595 1103 26-27 0.051063742803691525 1103 28-29 0.0059315488666698 1103 30-31 -0.036604613655875085 1103 32-33 -0.0462886209108504 1103 34-35 0.007829560347104803 1103 36-37 -0.0037821787213800917 1103 38-39 0.0019818058304821307 1103 40-41 -0.002991631940332695 1103 42-43 -0.050562622875936825 1103 44-45 -0.1328492110934576 1103 46-47 -0.1594157918924637 1103 48-49 -0.06933136696703457 1103 50-51 -0.07072165114632156 1103 52-53 -0.07938342747816307 1103 54-55 -0.10218985058973118 1103 56-57 -0.1777508292590042 1103 58-59 -0.24174387490302252 1103 60-61 -0.2330550111108849 1103 62-63 -0.24382802051265173 1103 64-65 -0.2502576743995135 1103 66-67 -0.22308039203726793 1103 68-69 -0.17336681819071487 1103 70-71 -0.11717116145931072 1103 72-73 -0.13368726891999216 1103 74-75 -0.12669617472513295 1103 76-77 -0.19213139435089488 1103 78-79 -0.134100690017668 1103 80-81 -0.1809349130576905 1103 82-83 -0.07118470669639265 1103 84-85 -0.1523422217379533 1103 86-87 -0.1788406614124085 1103 88-89 -0.12910165405784113 1103 90-91 -0.04431490806749849 1103 92-93 -0.047168749774449736 1103 94-95 -0.10974337047073135 1103 96-97 -0.11648949728581925 1103 98-99 -0.07147084215161925 1103 100-101 -0.0679526788817455 1103 102-103 -0.14987083694299486 1103 104-105 -0.17180268762387385 1103 106-107 -0.09419083600889877 1103 108-109 -0.1845644465729137 1103 110-111 -0.12176715195084853 1103 112-113 -0.14468460263413618 1103 114-115 -0.14095068150938062 1103 116-117 -0.11101632576023235 1103 118-119 -0.11245621325667798 1103 120-121 -0.07822508793448435 1103 122-123 -0.12774003896260666 1103 124-125 -0.08587597040006045 1104 1 0.033629191191906216 1104 2 0.022966590510286267 1104 3 0.0157074177749692 1104 4 0.03491773427216316 1104 5 -0.02519250400980866 1104 6 0.02306701705879277 1104 7 0.0020795387485961214 1104 8 -0.019174956137185006 1104 9 -0.058634642030320094 1104 10-11 0.04929634400559735 1104 12-13 0.04993771076041753 1104 14-15 -1.929659744561718E-4 1104 16-17 0.03997730445860981 1104 18-19 0.032119508275471276 1104 20-21 0.11390198423040943 1104 22-23 0.09140190697414852 1104 24-25 0.05805161149040572 1104 26-27 0.05117562416875643 1104 28-29 0.16272100412066237 1104 30-31 0.13650702505740497 1104 32-33 0.16746370571299707 1104 34-35 0.12330965490865253 1104 36-37 0.18005851965082087 1104 38-39 0.12587167388311826 1104 40-41 0.12416778738085554 1104 42-43 0.1423183803947765 1104 44-45 0.13683435353401308 1104 46-47 0.14789449291099288 1104 48-49 0.1691069290071212 1104 50-51 0.042932161756070286 1104 52-53 0.031192412830804983 1104 54-55 0.12069017916780922 1104 56-57 0.19312492575858187 1104 58-59 0.05724606885117112 1104 60-61 0.11851147330055056 1104 62-63 0.1579292434212931 1104 64-65 0.1717085606763149 1104 66-67 0.1589876405507269 1104 68-69 0.16937934624002082 1104 70-71 0.1732501953855774 1104 72-73 0.14851538455726399 1104 74-75 0.07822246427438984 1104 76-77 0.02643386517753754 1104 78-79 0.0569486554763472 1104 80-81 0.060073457702621624 1104 82-83 0.08036070914629079 1104 84-85 0.04604225494058767 1104 86-87 0.02078962153120756 1104 88-89 0.038337236195253865 1104 90-91 0.08385413159808053 1104 92-93 0.0062150076667464305 1104 94-95 0.04659725161872785 1104 96-97 0.060283786025408403 1104 98-99 0.019308099160070924 1104 100-101 0.09214613997070131 1104 102-103 0.09592881695127886 1104 104-105 0.10805815811206543 1104 106-107 0.136691399409834 1104 108-109 0.17388862645946546 1104 110-111 0.15703786788994023 1104 112-113 0.16230369631868058 1104 114-115 0.20708950569574824 1104 116-117 0.15910350354076996 1104 118-119 0.16929455574609165 1104 120-121 0.13789570906767068 1104 122-123 0.14248346222668573 1104 124-125 0.06112444661230043 1105 1 -0.01567049568851786 1105 2 0.04441957607810565 1105 3 -0.02387512731716157 1105 4 0.005761765798496299 1105 5 -0.06157741038273201 1105 6 -0.01640677736695295 1105 7 -0.0526473174840163 1105 8 0.019548089562938742 1105 9 0.01669024348689163 1105 10-11 0.03794311516850257 1105 12-13 0.07095555169055956 1105 14-15 0.02383667213143781 1105 16-17 0.011359126486581772 1105 18-19 -0.014593800974651572 1105 20-21 0.010213405818831234 1105 22-23 0.07326482292592829 1105 24-25 0.01930471446919313 1105 26-27 0.05454426834183579 1105 28-29 0.037267030426683334 1105 30-31 -0.016093585280863465 1105 32-33 0.004343810942756932 1105 34-35 0.08220481344540786 1105 36-37 0.14489414784564048 1105 38-39 0.08936367765674191 1105 40-41 0.20207117107408124 1105 42-43 0.20210647276469018 1105 44-45 0.1382875094684124 1105 46-47 0.18818988016487737 1105 48-49 0.22655652711946317 1105 50-51 0.2145177138183172 1105 52-53 0.28414135183744094 1105 54-55 0.19747723585089716 1105 56-57 0.3827854490537277 1105 58-59 0.3508473840811632 1105 60-61 0.396939089155147 1105 62-63 0.40182887763005226 1105 64-65 0.4700930595891677 1105 66-67 0.3791453432141907 1105 68-69 0.373397016093719 1105 70-71 0.34580584314415574 1105 72-73 0.3242766214754269 1105 74-75 0.29901357568206066 1105 76-77 0.2271973204105322 1105 78-79 0.2657661907404574 1105 80-81 0.23965644290840515 1105 82-83 0.26320905378652526 1105 84-85 0.19217518204428075 1105 86-87 0.17692044034847498 1105 88-89 0.21270927563227815 1105 90-91 0.2902783003688896 1105 92-93 0.1359015970485089 1105 94-95 0.20077176520438655 1105 96-97 0.15942755199284164 1105 98-99 0.13295176468220404 1105 100-101 0.2657254069259025 1105 102-103 0.18979718179414817 1105 104-105 0.2673911587573272 1105 106-107 0.2246198624832303 1105 108-109 0.21594652696111893 1105 110-111 0.21382914114586526 1105 112-113 0.17496299794348147 1105 114-115 0.21172503834330314 1105 116-117 0.2205469911717941 1105 118-119 0.23624194867601034 1105 120-121 0.05218832928436967 1105 122-123 0.10395101413701724 1105 124-125 0.02108751965625899 1106 1 0.028165285891226688 1106 2 0.07443039815403552 1106 3 0.04283860591105082 1106 4 0.032114282797195415 1106 5 -0.02248740701555363 1106 6 0.05542215346459756 1106 7 0.08069894876122419 1106 8 0.061099041309780944 1106 9 0.05172536327106059 1106 10-11 0.07770579161614677 1106 12-13 0.10444946098188268 1106 14-15 -0.007646631248348967 1106 16-17 0.028893591542079378 1106 18-19 0.03617157337079391 1106 20-21 0.03336745204198621 1106 22-23 0.05598906229750611 1106 24-25 0.03759457705238134 1106 26-27 0.003770857115419801 1106 28-29 0.04022293982054492 1106 30-31 0.07868819261576476 1106 32-33 0.12945144279263587 1106 34-35 0.10605786046254195 1106 36-37 0.049353785382301396 1106 38-39 0.06805388776574972 1106 40-41 0.09330854589619264 1106 42-43 0.0897050111608948 1106 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-0.04298570449868322 2216 8 -0.06366906959321028 2216 9 -0.05620877686061476 2216 10-11 -0.11191107263760358 2216 12-13 -0.0971919885633099 2216 14-15 -0.10120364736562237 2216 16-17 -0.08353813155934375 2216 18-19 -0.1469064109044922 2216 20-21 -0.15655784994659427 2216 22-23 -0.166313229114472 2216 24-25 -0.11790290497410894 2216 26-27 -0.1362509017647895 2216 28-29 -0.2190442121214886 2216 30-31 -0.35663970473614626 2216 32-33 -0.32865990883760077 2216 34-35 -0.2852957886362617 2216 36-37 -0.2193936209367493 2216 38-39 -0.2253679765440708 2216 40-41 -0.1945154539243532 2216 42-43 -0.1690355534484027 2216 44-45 -0.16035423995832332 2216 46-47 -0.15854307065161777 2216 48-49 -0.09905517072800052 2216 50-51 -0.19022640432893212 2216 52-53 -0.13392155456767796 2216 54-55 -0.20879080519794968 2216 56-57 -0.3312578346254611 2216 58-59 -0.34652976557865145 2216 60-61 -0.3156405368579769 2216 62-63 -0.34189263762971933 2216 64-65 -0.29004154418402806 2216 66-67 -0.3826186476227562 2216 68-69 -0.3529967030816934 2216 70-71 -0.396918328123256 2216 72-73 -0.35805921568060484 2216 74-75 -0.3192017058924108 2216 76-77 -0.3574256851582973 2216 78-79 -0.3673200513226291 2216 80-81 -0.314377390086122 2216 82-83 -0.2154394184098578 2216 84-85 -0.2697795916357961 2216 86-87 -0.21900674828970068 2216 88-89 -0.2508299715316653 2216 90-91 -0.24580008780998952 2216 92-93 -0.17993452031803514 2216 94-95 -0.18182995806176194 2216 96-97 -0.182737111349347 2216 98-99 -0.22169430831862513 2216 100-101 -0.19108884488918676 2216 102-103 -0.18414398198690307 2216 104-105 -0.30532518259500563 2216 106-107 -0.23341995366214263 2216 108-109 -0.2627607837369794 2216 110-111 -0.35564908518433924 2216 112-113 -0.32029408236564905 2216 114-115 -0.3948835925971821 2216 116-117 -0.4544901324415562 2216 118-119 -0.4301788441999008 2216 120-121 -0.44471988151155273 2216 122-123 -0.5869015677375238 2216 124-125 -0.6464967460951669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 190.0 3 4.0 4 24.0 5 60.0 6 134.0 7 320.0 8 611.0 9 1163.0 10 2882.0 11 4412.0 12 4804.0 13 7638.0 14 13939.0 15 15098.0 16 5010.0 17 3125.0 18 2629.0 19 2385.0 20 2451.0 21 2423.0 22 2520.0 23 2709.0 24 3319.0 25 4347.0 26 5543.0 27 7114.0 28 9567.0 29 12160.0 30 15451.0 31 20105.0 32 26281.0 33 34995.0 34 50258.0 35 80039.0 36 174925.0 37 619576.0 38 666180.0 39 7615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.194089231149654 21.084270183867037 15.742787060423547 22.97885352455976 2 12.860222317144645 21.42713655473547 44.54935579683511 21.163285331284776 3 17.588517918814837 27.925514595426282 30.414413638806938 24.071553846951943 4 11.109621049819923 18.75440809798643 40.7605162455312 29.375454606662448 5 11.438555806959 39.54105177523132 35.80223919778844 13.218153220021245 6 27.603164669432662 39.143689369682 20.277637049766945 12.975508911118396 7 25.641692135677257 33.34751650932723 24.137171731219436 16.87361962377608 8 23.391920335804627 38.48706902736525 21.810689368578252 16.31032126825187 9 25.527785228084234 17.361035228360173 23.000475715864074 34.110703827691516 10-11 22.820095518447513 27.37413673023158 30.927243066524063 18.87852468479685 12-13 25.606703289061954 25.19232828147368 30.328707520836023 18.872260908628338 14-15 20.226892184683713 29.2690531929806 27.948086264615018 22.555968357720673 16-17 18.925085747555883 30.328724258487146 31.110620555682793 19.635569438274178 18-19 19.92291968128141 27.815774340703065 33.213631742941246 19.04767423507428 20-21 21.04125521509265 27.799938021836958 31.441279277852303 19.7175274852181 22-23 19.779304940252565 29.602801320638356 31.705171488449935 18.912722250659147 24-25 20.26022540764214 28.128714805580113 32.89133700440286 18.71972278237489 26-27 18.755594628273858 29.425785565831458 32.361509840475144 19.45710996541954 28-29 18.968011143450962 29.346260442846216 31.07365538524707 20.612073028455754 30-31 19.81629751777864 28.12349959106096 32.070644357689766 19.98955853347064 32-33 18.89381160989533 29.25023427074745 32.58786118809761 19.26809293125961 34-35 19.303412902606283 29.393611279432854 32.41846329427165 18.884512523689214 36-37 18.89392198480579 28.82760873860241 32.092608964870976 20.18586031172082 38-39 20.072643247318165 29.41345116958774 31.14876551181398 19.365140071280116 40-41 21.28202114121035 27.685504352634595 32.17861861384565 18.853855892309408 42-43 19.263126060288986 29.618362190853674 31.977625901900986 19.14088584695636 44-45 19.031918216606346 29.67796464250118 31.171364774730325 20.118752366162145 46-47 20.710748198405522 28.375816636368757 30.657348816725772 20.25608634849995 48-49 20.215440787723658 28.956885352476757 30.20966817990669 20.618005679892892 50-51 19.51922344627998 30.276527781916833 30.180225672541923 20.02402309926126 52-53 19.49179528103108 29.601888735467764 30.743772371614664 20.162543611886495 54-55 19.68864893383355 28.874545669274827 30.98002986745077 20.45677552944085 56-57 20.188592090754668 29.220460638651307 29.281111651948173 21.309835618645852 58-59 20.52013072804395 29.531276386502032 28.945627111610005 21.002965773844014 60-61 19.751259101791057 28.376202948555356 31.27688318912851 20.595654760525075 62-63 20.24441420171898 28.302224164820643 31.084720470020518 20.368641163439857 64-65 20.451256783917934 29.226338102633214 29.700950217604138 20.621454895844714 66-67 20.464612148083393 30.082267939510132 29.060196268665777 20.392923643740694 68-69 21.025151130846144 28.296650231842502 30.87144854928737 19.806750088023993 70-71 21.60081147634169 28.308074035074938 30.135441052623445 19.955673435959927 72-73 20.718281286044306 29.335140170617535 29.628295932794924 20.318282610543232 74-75 19.900541168185978 29.87989554118474 30.698932564240955 19.520630726388326 76-77 20.38525258746384 28.443062550565507 30.98905301638074 20.182631845589917 78-79 20.050430296588424 28.421429068115668 30.525726736004184 21.002413899291724 80-81 20.45249850166059 29.25812607684522 30.206688057324314 20.082687364169878 82-83 19.99996136878134 29.199461811936605 31.019571679122475 19.78100514015958 84-85 20.003272616095092 28.891019124660733 30.928291628173415 20.17741663107076 86-87 19.056090321996727 30.304480227990418 29.53444966517771 21.104979784835148 88-89 20.148525998258286 28.79800066887196 30.188172666094925 20.865300666774832 90-91 20.139260831497648 29.03704782931304 30.8750214887399 19.948669850449406 92-93 19.532606404173055 30.07545228878933 30.60596929590741 19.785972011130205 94-95 19.45088685423714 30.031713672420086 30.187067344851215 20.33033212849156 96-97 20.57369015334386 29.21902576481535 29.89303015552929 20.3142539263115 98-99 20.50142205805617 28.791936363764297 30.781382753127573 19.92525882505195 100-101 19.918096275094086 28.934459636022076 30.71905686820487 20.428387220678964 102-103 19.539901297045674 29.775897108309728 30.230918555575943 20.453283039068655 104-105 19.99756067072725 29.09573456786206 30.062222212410944 20.84448254899975 106-107 19.973304059968452 28.542303434101264 29.936596452536197 21.547796053394087 108-109 19.578990879432627 29.516995966067395 29.62430812081765 21.27970503368233 110-111 19.363821299742597 29.631475422864483 30.134392757328104 20.870310520064812 112-113 19.856677786905898 28.600116510009183 29.84483085489702 21.6983748481879 114-115 19.65841329656584 29.17394828101471 28.836241425907726 22.331396996511724 116-117 20.06891885478144 29.085296464762973 28.115035105127745 22.730749575327845 118-119 20.011031990565137 28.368385703555678 29.48245670674938 22.1381255991298 120-121 19.498857201040728 28.36307535574671 30.242767861010922 21.89529958220164 122-123 20.150326187794324 29.059301001714687 29.241310534864095 21.54906227562689 124-125 20.558260075817323 29.0334312553015 28.497141535783644 21.911167133097535 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 31.0 1 29.0 2 57.5 3 82.0 4 54.0 5 41.0 6 99.0 7 257.5 8 474.5 9 574.0 10 518.0 11 506.5 12 609.0 13 740.0 14 919.5 15 1176.5 16 1445.0 17 1754.5 18 2089.0 19 2455.0 20 2935.0 21 3638.0 22 4594.5 23 5769.5 24 7234.0 25 9248.0 26 12067.5 27 15573.5 28 19561.0 29 24367.5 30 30576.5 31 38684.0 32 49477.0 33 63469.5 34 79887.5 35 95822.5 36 107968.0 37 111292.0 38 104145.5 39 91360.5 40 79724.5 41 72011.5 42 65956.0 43 61572.0 44 58431.0 45 55957.5 46 54106.0 47 53585.5 48 54836.0 49 51951.0 50 45927.0 51 41025.0 52 36786.5 53 33174.0 54 29472.5 55 25515.0 56 21499.0 57 17649.5 58 14312.0 59 11547.5 60 8884.5 61 6803.0 62 5260.0 63 3867.5 64 2775.0 65 2109.5 66 1536.5 67 1147.5 68 854.0 69 553.5 70 420.0 71 348.0 72 261.5 73 187.0 74 142.5 75 110.0 76 62.0 77 26.5 78 12.0 79 6.5 80 4.0 81 2.0 82 2.0 83 2.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.5 97 2.5 98 7.0 99 6.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014459113270044361 2 0.0 3 0.0 4 0.0 5 0.14392888323769346 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.5187455229176944E-5 18-19 0.0 20-21 0.004277027780261213 22-23 1.931560933021193E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 5.794682799063579E-4 92-93 0.0 94-95 6.346557351355348E-4 96-97 0.0 98-99 0.0010761553769689505 100-101 2.7593727614588472E-5 102-103 1.931560933021193E-4 104-105 0.0017935922949482506 106-107 0.006622494627501234 108-109 1.3796863807294236E-4 110-111 0.004304621507875802 112-113 0.007974587280616069 114-115 0.004387402690719567 116-117 0.0011037491045835389 118-119 1.6556236568753083E-4 120-121 4.139059142188271E-4 122-123 7.174369179793003E-4 124-125 0.001048561649354362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1812006.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.86506184895558 #Duplication Level Percentage of deduplicated Percentage of total 1 84.40635522291454 60.658679385396674 2 10.609229599983623 15.248658827451866 3 2.5682354892538775 5.536992068337377 4 0.9480400509007268 2.725238275730709 5 0.4391002265416757 1.5777982469153962 6 0.25877058203508735 1.115793832958507 7 0.15427906331503663 0.7761092098993829 8 0.10810533104658093 0.6215197041491485 9 0.07261246477348907 0.4696469344756709 >10 0.3873123289435047 4.966638101840778 >50 0.026232757511507932 1.315280292623053 >100 0.018656902093671622 2.5143909790617203 >500 0.002379235032187463 1.2287121719545606 >1k 6.907456545060368E-4 1.24454196920513 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4074 0.2248336926036669 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3151 0.17389567142713655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2839 0.15667718539563333 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2716 0.1498891284024446 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 2161 0.11926009075025137 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 2126 0.11732852981723017 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2035 0.11230647139137508 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1881 0.10380760328608184 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.5187455229176944E-5 0.0 5 0.0 0.0 0.0 5.5187455229176944E-5 0.0 6 0.0 0.0 0.0 5.5187455229176944E-5 0.0 7 0.0 0.0 0.0 5.5187455229176944E-5 0.0 8 0.0 0.0 0.0 1.6556236568753083E-4 0.0 9 0.0 0.0 0.0 2.2074982091670778E-4 0.0 10-11 0.0 0.0 0.0 2.759372761458847E-4 0.0 12-13 0.0 0.0 0.0 3.5871845898965016E-4 0.0 14-15 0.0 0.0 0.0 5.242808246771809E-4 0.0 16-17 0.0 0.0 0.0 6.070620075209464E-4 0.0 18-19 0.0 0.0 0.0 6.070620075209464E-4 0.0 20-21 0.0 0.0 0.0 6.346557351355349E-4 0.0 22-23 0.0 0.0 0.0 8.002181008230657E-4 2.7593727614588472E-5 24-25 0.0 0.0 0.0 9.105930112814196E-4 5.5187455229176944E-5 26-27 0.0 0.0 0.0 0.0011313428321981273 5.5187455229176944E-5 28-29 0.0 0.0 0.0 0.0016832173844898968 5.5187455229176944E-5 30-31 0.0 0.0 0.0 0.00361477831751109 5.5187455229176944E-5 32-33 0.0 0.0 0.0 0.007284744090251356 5.5187455229176944E-5 34-35 0.0 0.0 0.0 0.013603707713992119 5.5187455229176944E-5 36-37 0.0 0.0 0.0 0.024061730479921148 5.5187455229176944E-5 38-39 0.0 0.0 0.0 0.04299102762352884 5.5187455229176944E-5 40-41 0.0 0.0 0.0 0.0665560710063874 5.5187455229176944E-5 42-43 0.0 0.0 0.0 0.08934849001603748 5.5187455229176944E-5 44-45 0.0 0.0 0.0 0.11669387408209464 5.5187455229176944E-5 46-47 2.7593727614588472E-5 0.0 0.0 0.15121362732794486 8.278118284376542E-5 48-49 5.5187455229176944E-5 0.0 0.0 0.18990003344359785 1.1037491045835389E-4 50-51 5.5187455229176944E-5 0.0 0.0 0.23258753006336624 1.1037491045835389E-4 52-53 5.5187455229176944E-5 0.0 0.0 0.27414368385093646 1.1037491045835389E-4 54-55 5.5187455229176944E-5 0.0 0.0 0.32160489534802866 1.1037491045835389E-4 56-57 5.5187455229176944E-5 0.0 0.0 0.37704069412573693 1.1037491045835389E-4 58-59 5.5187455229176944E-5 0.0 0.0 0.4421894850237803 1.1037491045835389E-4 60-61 5.5187455229176944E-5 0.0 0.0 0.5204728902663678 1.1037491045835389E-4 62-63 5.5187455229176944E-5 0.0 0.0 0.5983975770499657 1.1037491045835389E-4 64-65 5.5187455229176944E-5 0.0 0.0 0.676156701467876 1.1037491045835389E-4 66-67 5.5187455229176944E-5 0.0 0.0 0.7666641280437261 1.1037491045835389E-4 68-69 5.5187455229176944E-5 0.0 0.0 0.8792465367112471 1.1037491045835389E-4 70-71 5.5187455229176944E-5 0.0 0.0 1.0082472133094482 1.6556236568753083E-4 72-73 5.5187455229176944E-5 0.0 0.0 1.134322954780503 2.759372761458847E-4 74-75 5.5187455229176944E-5 0.0 0.0 1.2586051039566093 2.759372761458847E-4 76-77 5.5187455229176944E-5 0.0 0.0 1.3989468026044065 2.759372761458847E-4 78-79 5.5187455229176944E-5 0.0 0.0 1.5603149216945198 2.759372761458847E-4 80-81 5.5187455229176944E-5 0.0 0.0 1.7358110293233024 2.759372761458847E-4 82-83 5.5187455229176944E-5 0.0 0.0 1.9158876957361068 2.759372761458847E-4 84-85 5.5187455229176944E-5 0.0 0.0 2.100020640108256 2.759372761458847E-4 86-87 5.5187455229176944E-5 0.0 0.0 2.2876303941598426 2.759372761458847E-4 88-89 5.5187455229176944E-5 0.0 0.0 2.493838320623662 2.759372761458847E-4 90-91 5.5187455229176944E-5 0.0 0.0 2.7230318221904346 2.759372761458847E-4 92-93 5.5187455229176944E-5 0.0 0.0 2.9630420649821247 2.759372761458847E-4 94-95 5.5187455229176944E-5 0.0 0.0 3.208709021934806 2.759372761458847E-4 96-97 5.5187455229176944E-5 0.0 0.0 3.4559212276339037 2.759372761458847E-4 98-99 1.1037491045835389E-4 0.0 0.0 3.7297889742086947 2.759372761458847E-4 100-101 1.1037491045835389E-4 0.0 0.0 4.030643386390553 2.759372761458847E-4 102-103 1.1037491045835389E-4 0.0 0.0 4.349047409335289 2.759372761458847E-4 104-105 1.1037491045835389E-4 0.0 0.0 4.670183211313869 3.3112473137506167E-4 106-107 1.6556236568753083E-4 0.0 0.0 4.999486756666368 3.3112473137506167E-4 108-109 1.6556236568753083E-4 0.0 0.0 5.339662230699015 3.3112473137506167E-4 110-111 1.6556236568753083E-4 0.0 0.0 5.716178644000075 3.3112473137506167E-4 112-113 1.6556236568753083E-4 0.0 0.0 6.134306398543934 3.3112473137506167E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9590 0.0 38.331013 1 GTACATG 8140 0.0 34.912243 1 TACATGG 8090 0.0 33.503227 2 ACATGGG 7755 0.0 32.569252 3 ATCAACG 11190 0.0 32.47308 3 TCAACGC 11335 0.0 32.11023 4 CAACGCA 11510 0.0 31.622023 5 TATCAAC 11605 0.0 31.46582 2 AACGCAG 11660 0.0 31.227482 6 GGTATCA 6610 0.0 30.19445 1 TAGGACC 3055 0.0 29.053522 4 ACGCAGA 12980 0.0 27.868464 7 CATGGGG 3960 0.0 27.82917 4 CGCAGAG 13090 0.0 27.543373 8 AGAGTAC 10610 0.0 26.383255 10-11 GACGGAC 115 9.982164E-4 25.89977 3 CGCGATC 70 1.0570777E-4 25.498072 22-23 AGTACTT 6825 0.0 25.323727 12-13 GCAGAGT 14405 0.0 24.905092 9 GAGTACT 6470 0.0 24.506264 12-13 >>END_MODULE