FastQCFastQC Report
Fri 27 May 2016
SRR939923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101822
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50980.24255146249301798No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA49840.237127596913535No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC39970.19016833965959057No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA33170.15781545725565724No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27330.1300300406028674No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA26770.12736568558136704No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21570.10262524609600623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4300.02749.0613108-109
CGTCGCG401.8189894E-122462.7004108-109
CCGCGCC3900.02083.8235108-109
CGCGTCT504.800313E-91836.9734110-111
CCGCTCG1450.01698.414108-109
CGTCGTC701.843182E-81312.1238110-111
CGACGCC602.3810571E-81231.3502108-109
CTCCGCC2550.01200.6362110-111
CGTCGCC1950.01136.631108-109
CCGCGCA653.2789103E-81136.631108-109
CCGCGAC451.6568927E-51094.5336108-109
CCGCGCG1150.01070.7394108-109
TTGTTCG3150.0938.1716108-109
CTTCGCT1750.0874.7492110-111
CAGCTCG1551.8189894E-12794.4195108-109
CTCCGCT2151.8189894E-12712.00525110-111
CCGTTCG706.231919E-5703.62866108-109
CCGCACG1052.2298445E-7703.62866108-109
CGTCGTT906.886045E-5680.3606110-111
AAGCGCG1102.685465E-7671.64557108-109
Produced by FastQC (version 0.11.5)