##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939923_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101822 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18103673860108 34.0 31.0 34.0 30.0 34.0 2 32.291893414380475 34.0 31.0 34.0 30.0 34.0 3 32.38376275441022 34.0 31.0 34.0 30.0 34.0 4 35.629664167565096 37.0 37.0 37.0 35.0 37.0 5 35.67064194779577 37.0 37.0 37.0 35.0 37.0 6 35.70618587111563 37.0 37.0 37.0 35.0 37.0 7 35.703701835835766 37.0 37.0 37.0 35.0 37.0 8 35.71319645526596 37.0 37.0 37.0 35.0 37.0 9 37.44080707119823 39.0 39.0 39.0 35.0 39.0 10-11 37.4204192838404 39.0 39.0 39.0 35.0 39.0 12-13 37.387880372362645 39.0 39.0 39.0 35.0 39.0 14-15 38.944120862756215 41.0 40.0 41.0 36.0 41.0 16-17 38.93481536495479 41.0 40.0 41.0 36.0 41.0 18-19 38.93312112062772 41.0 40.0 41.0 36.0 41.0 20-21 38.97433441081119 41.0 40.0 41.0 36.0 41.0 22-23 38.94581582074981 41.0 40.0 41.0 36.0 41.0 24-25 38.95870202138906 41.0 40.0 41.0 36.0 41.0 26-27 38.91549617427165 41.0 39.0 41.0 35.5 41.0 28-29 38.85872447809567 41.0 39.0 41.0 35.0 41.0 30-31 38.775115114410255 41.0 39.0 41.0 35.0 41.0 32-33 38.69169677546434 41.0 39.0 41.0 35.0 41.0 34-35 38.64517570945589 41.0 39.0 41.0 35.0 41.0 36-37 38.80205007845574 41.0 39.0 41.0 35.0 41.0 38-39 38.75587181026747 41.0 39.0 41.0 35.0 41.0 40-41 38.67817802839632 41.0 39.0 41.0 35.0 41.0 42-43 38.63455944413942 41.0 39.0 41.0 34.5 41.0 44-45 38.56165579197477 41.0 39.0 41.0 34.5 41.0 46-47 38.46199654395092 41.0 39.0 41.0 34.0 41.0 48-49 38.38494220728492 41.0 39.0 41.0 34.0 41.0 50-51 38.281272391287175 41.0 39.0 41.0 33.0 41.0 52-53 38.20525667730188 40.5 38.5 41.0 33.0 41.0 54-55 38.078806625870314 40.0 38.0 41.0 33.0 41.0 56-57 37.89235482357688 40.0 38.0 41.0 33.0 41.0 58-59 37.74161299101446 40.0 38.0 41.0 33.0 41.0 60-61 37.54322797077964 40.0 37.0 41.0 32.0 41.0 62-63 37.31577126892763 40.0 37.0 41.0 32.0 41.0 64-65 37.03955853540404 40.0 36.5 41.0 31.0 41.0 66-67 36.73335919978 39.0 36.0 41.0 31.0 41.0 68-69 36.32855898358662 39.0 35.5 41.0 31.0 41.0 70-71 35.8978655185834 38.5 35.0 40.0 30.0 41.0 72-73 35.501749434538226 37.0 35.0 40.0 30.0 41.0 74-75 35.078441228610224 37.0 35.0 39.0 30.0 41.0 76-77 34.58583338646184 36.5 35.0 39.0 29.0 40.5 78-79 34.102093326647065 36.0 34.0 38.5 29.0 39.5 80-81 33.644932824949024 35.5 34.0 37.0 28.5 39.0 82-83 33.415743102888825 35.0 34.0 37.0 29.0 39.0 84-85 32.96877233181497 35.0 34.0 37.0 29.0 38.5 86-87 32.33269515686866 35.0 34.0 36.0 27.5 37.0 88-89 16.6342406730922 18.0 17.0 18.5 2.0 19.5 90-91 2.8300224757377173 2.0 2.0 2.0 2.0 2.0 92-93 2.3175071438019015 2.0 2.0 2.0 2.0 2.0 94-95 2.107493403342433 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1567122844632749 1101 2 0.14407032303208211 1101 3 0.26277577312714584 1101 4 0.2573877145712302 1101 5 0.24387251156600342 1101 6 0.2705576288790823 1101 7 0.24779449075160898 1101 8 0.2745311970884856 1101 9 0.3354495339343302 1101 10-11 0.28146489392565144 1101 12-13 0.3337960268549409 1101 14-15 0.3786029948287677 1101 16-17 0.39164803278949734 1101 18-19 0.36516868112746437 1101 20-21 0.35046671751796765 1101 22-23 0.3326818257378932 1101 24-25 0.3120196716232613 1101 26-27 0.34450725544657956 1101 28-29 0.28820800044627504 1101 30-31 0.2576037336988364 1101 32-33 0.3391489043232525 1101 34-35 0.3701753585106786 1101 36-37 0.3596167763821896 1101 38-39 0.33173131162858027 1101 40-41 0.3081985362379882 1101 42-43 0.340309928458268 1101 44-45 0.32788062190707734 1101 46-47 0.3309758683167132 1101 48-49 0.3117512512447007 1101 50-51 0.3606793165834148 1101 52-53 0.3650376612472641 1101 54-55 0.4259984367887881 1101 56-57 0.39077643655200944 1101 58-59 0.4029097847633665 1101 60-61 0.41875860613743043 1101 62-63 0.33916851929745206 1101 64-65 0.4175458362307225 1101 66-67 0.45686204636439953 1101 68-69 0.3934644554214728 1101 70-71 0.3852213017894641 1101 72-73 0.327279054597426 1101 74-75 0.32061427526799946 1101 76-77 0.29229258230765254 1101 78-79 0.31060173066187247 1101 80-81 0.27786157200524997 1101 82-83 0.37712976260099396 1101 84-85 0.3476380347742847 1101 86-87 0.2705506641025863 1101 88-89 0.5662180041751128 1101 90-91 -0.0895263602202232 1101 92-93 -0.1604971223886178 1101 94-95 0.016628829064562645 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.04530333646832929 1102 2 0.09035374675087837 1102 3 0.11540964991470304 1102 4 0.11341063029563969 1102 5 0.17820916878274318 1102 6 0.028912637128151175 1102 7 0.10470128647379084 1102 8 0.09566391891097226 1102 9 0.0595150157214519 1102 10-11 0.1377364231926137 1102 12-13 0.09976382908268988 1102 14-15 0.13767661630174644 1102 16-17 0.16870577057984804 1102 18-19 0.12599430874883666 1102 20-21 0.08800613780492483 1102 22-23 0.19191222474182013 1102 24-25 0.15789615860808226 1102 26-27 0.19410908141853156 1102 28-29 0.23165495884803278 1102 30-31 0.22059105072235496 1102 32-33 0.2528260852858466 1102 34-35 0.2072634578286383 1102 36-37 0.19418818375599045 1102 38-39 0.2265161818477921 1102 40-41 0.22139917818645216 1102 42-43 0.24599087274743425 1102 44-45 0.23602903479912385 1102 46-47 0.27483480468353605 1102 48-49 0.199665066167789 1102 50-51 0.24039905029226816 1102 52-53 0.2237519285495111 1102 54-55 0.21103374442991907 1102 56-57 0.25741887201830593 1102 58-59 0.19844292110822437 1102 60-61 0.15974580045929088 1102 62-63 0.17940453451816296 1102 64-65 0.11715980158224681 1102 66-67 0.08693425011002631 1102 68-69 0.09114295661590432 1102 70-71 0.12539958064659373 1102 72-73 0.15901274823610834 1102 74-75 0.039776743683020754 1102 76-77 -0.03148452679361924 1102 78-79 0.02653091515713868 1102 80-81 -0.021389115575871642 1102 82-83 -0.036779386329811814 1102 84-85 -0.10937851473131133 1102 86-87 0.0028810896918756157 1102 88-89 0.9420564060858361 1102 90-91 0.050108072053928865 1102 92-93 -0.08530983589783325 1102 94-95 0.05799486476884441 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.07238717545734374 1103 2 0.07118450759532635 1103 3 0.08333698426320524 1103 4 -0.0016724877204126187 1103 5 -0.054751558165470726 1103 6 -0.015490883486016571 1103 7 0.037091356157738176 1103 8 0.02660130909688263 1103 9 0.07505876611171658 1103 10-11 0.016253607631064426 1103 12-13 0.07644957083230253 1103 14-15 0.08010178547527858 1103 16-17 0.10385215974435624 1103 18-19 0.09813309188449892 1103 20-21 0.08870376351884346 1103 22-23 0.12859219405882527 1103 24-25 0.08318223927447832 1103 26-27 0.1090464301286076 1103 28-29 0.11586715055386776 1103 30-31 0.07585573487481412 1103 32-33 0.09509721277662209 1103 34-35 0.09608886692387841 1103 36-37 0.09728184757116054 1103 38-39 0.08976889407071553 1103 40-41 0.13519081410706946 1103 42-43 0.14504440345709213 1103 44-45 0.09010670125051234 1103 46-47 0.11832810177945419 1103 48-49 0.10056256207603553 1103 50-51 0.08335954251464273 1103 52-53 0.044800820347276726 1103 54-55 0.06021595275798575 1103 56-57 0.1583537417500196 1103 58-59 0.07518929277084396 1103 60-61 0.0794320175857095 1103 62-63 0.035487511698704566 1103 64-65 0.019938864401716216 1103 66-67 0.06008377867571113 1103 68-69 0.05692385986829862 1103 70-71 0.05810163060080953 1103 72-73 -0.015678655647761275 1103 74-75 0.022063203556982103 1103 76-77 0.07021146996852679 1103 78-79 0.08225579869437638 1103 80-81 0.11008869098177598 1103 82-83 0.07895669112333081 1103 84-85 0.11501135760061487 1103 86-87 0.18959085551752253 1103 88-89 0.7748861423405842 1103 90-91 -0.6018855137775638 1103 92-93 -0.34836953739037035 1103 94-95 -0.12077039981731597 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.10099807170244901 1104 2 0.1723152516885662 1104 3 0.20516092364837846 1104 4 0.18075048131148463 1104 5 0.19414049948623813 1104 6 0.20950605774331876 1104 7 0.212719293967929 1104 8 0.21434220119012792 1104 9 0.27820318235367836 1104 10-11 0.31493883677190837 1104 12-13 0.32517178329117513 1104 14-15 0.3201404260908518 1104 16-17 0.2943282601788155 1104 18-19 0.3265494050314288 1104 20-21 0.2894052230529809 1104 22-23 0.17272669543471153 1104 24-25 0.2751149262988335 1104 26-27 0.33218091370801517 1104 28-29 0.36006416207931125 1104 30-31 0.3147318757962623 1104 32-33 0.29291814455602605 1104 34-35 0.3337580169415588 1104 36-37 0.28095123784137144 1104 38-39 0.294728551666374 1104 40-41 0.3456028138312277 1104 42-43 0.2569663236087223 1104 44-45 0.2099091825252728 1104 46-47 0.2756024145571132 1104 48-49 0.2605005635753912 1104 50-51 0.2295520960208819 1104 52-53 0.20292986487630316 1104 54-55 0.25140952956204643 1104 56-57 0.12862215721783343 1104 58-59 0.06629316417344455 1104 60-61 0.11748412569045286 1104 62-63 0.09683716366621553 1104 64-65 0.08785831084208695 1104 66-67 0.05152962063848321 1104 68-69 0.04554284159798527 1104 70-71 0.0763597107606202 1104 72-73 0.12108109404469047 1104 74-75 0.08786659245242845 1104 76-77 0.11210906455127656 1104 78-79 0.11620086762238202 1104 80-81 0.10115574590840026 1104 82-83 0.17457975714606278 1104 84-85 0.2349766210886841 1104 86-87 0.2416241594125097 1104 88-89 0.8502952390133096 1104 90-91 -0.6916184997828538 1104 92-93 -0.34836953739037035 1104 94-95 -0.12077039981731597 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.14457399527649528 1105 2 -0.17321212676402808 1105 3 -0.1013102980174807 1105 4 -0.10141987350464632 1105 5 -0.164684676844999 1105 6 -0.22402864316660498 1105 7 -0.18015315528636933 1105 8 -0.19388048765927834 1105 9 -0.16178281883083656 1105 10-11 -0.1719983839247945 1105 12-13 -0.1948998260341881 1105 14-15 -0.21842469532194997 1105 16-17 -0.18038072598389476 1105 18-19 -0.14522790611916747 1105 20-21 -0.09505797621477541 1105 22-23 -0.30289544423039416 1105 24-25 -0.22210119387561633 1105 26-27 -0.11407036502993861 1105 28-29 -0.2300769029074985 1105 30-31 -0.24508748564237948 1105 32-33 -0.21634099505523352 1105 34-35 -0.25983806339010584 1105 36-37 -0.360431952811723 1105 38-39 -0.30103949872937363 1105 40-41 -0.29306756790570176 1105 42-43 -0.2701878787280094 1105 44-45 -0.2995265575274928 1105 46-47 -0.22542757521298995 1105 48-49 -0.2619842261731691 1105 50-51 -0.2821110709419088 1105 52-53 -0.30001769340722717 1105 54-55 -0.4168220431510292 1105 56-57 -0.36919671964642475 1105 58-59 -0.3858195801328037 1105 60-61 -0.373648701531863 1105 62-63 -0.3322187239125256 1105 64-65 -0.343290938067625 1105 66-67 -0.30341169201967233 1105 68-69 -0.31558002492869974 1105 70-71 -0.390764609489203 1105 72-73 -0.3081882754702008 1105 74-75 -0.36407655082007295 1105 76-77 -0.3315392421304679 1105 78-79 -0.32728564505731583 1105 80-81 -0.44780349600189595 1105 82-83 -0.3696150417555657 1105 84-85 -0.36399473518117276 1105 86-87 -0.16177782195829593 1105 88-89 0.575983334635989 1105 90-91 -0.8459231355444432 1105 92-93 -0.34836953739037035 1105 94-95 -0.12077039981731597 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.02984749121145569 1106 2 -0.0023009072518291873 1106 3 0.07240807596625842 1106 4 0.015858825682343536 1106 5 0.05159109551272678 1106 6 0.029767779597626998 1106 7 -0.07867673252214047 1106 8 0.008611870064299865 1106 9 -0.01116688387148912 1106 10-11 -0.07111122945325121 1106 12-13 -0.08933364717240266 1106 14-15 -0.045571361988621106 1106 16-17 -0.14352869346357267 1106 18-19 -0.13380026384274402 1106 20-21 -0.07063000873510106 1106 22-23 -0.11014747675071845 1106 24-25 -0.19102997436341695 1106 26-27 -0.3243623975502672 1106 28-29 -0.05073901672862746 1106 30-31 -0.04780878645539133 1106 32-33 0.06348079356264691 1106 34-35 -0.19013920160148245 1106 36-37 -0.11811260321822914 1106 38-39 -0.10165608409521809 1106 40-41 -0.06149943782440204 1106 42-43 -0.396807716126375 1106 44-45 -0.05351810224293985 1106 46-47 -0.23340155895282066 1106 48-49 -0.15199268145772749 1106 50-51 -0.18939042053541044 1106 52-53 -0.18526907552104888 1106 54-55 -0.14585749030549522 1106 56-57 -0.2604877765569995 1106 58-59 -0.16888299476694613 1106 60-61 -0.0716766690115378 1106 62-63 -0.048320187327156816 1106 64-65 -0.018046060018839682 1106 66-67 0.0075489583868275645 1106 68-69 -0.006777423302679608 1106 70-71 0.06630726042948965 1106 72-73 0.037617252985086225 1106 74-75 -0.06895459960055916 1106 76-77 -0.09458444538249466 1106 78-79 -0.003250523106089531 1106 80-81 -0.054641869985644576 1106 82-83 0.003906909463943009 1106 84-85 -0.06266920672589293 1106 86-87 0.08096429186284126 1106 88-89 0.6664496760994041 1106 90-91 -0.21888313554444316 1106 92-93 -0.3296495373903703 1106 94-95 -0.12077039981731597 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.06842541402287594 1107 2 -0.13562865121166823 1107 3 -0.03405312265313398 1107 4 -0.047880214167044244 1107 5 -0.027662271289905505 1107 6 0.051934632437287576 1107 7 -0.0954292897860256 1107 8 -0.08051791970664368 1107 9 -0.03159406931234088 1107 10-11 -0.04386218019062227 1107 12-13 -0.010600092104937175 1107 14-15 -0.03702735194782747 1107 16-17 -0.026240711034951403 1107 18-19 -0.04234949509470454 1107 20-21 -0.049223093140497554 1107 22-23 -0.011255729025585026 1107 24-25 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2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16108.0 3 2649.0 4 1911.0 5 1699.0 6 1724.0 7 2085.0 8 2388.0 9 2827.0 10 3477.0 11 4101.0 12 5016.0 13 5933.0 14 6792.0 15 7674.0 16 8481.0 17 9956.0 18 12627.0 19 15574.0 20 17840.0 21 24918.0 22 30100.0 23 39139.0 24 60156.0 25 102462.0 26 187756.0 27 558407.0 28 934143.0 29 34581.0 30 1298.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.32701151667458 20.46629067542351 14.918818054050249 24.28787975385166 2 12.74005568549684 20.489969568998823 44.949719383919714 21.82025536158462 3 18.67689129431029 25.697469071453394 29.548697883197434 26.076941751038884 4 11.375886673372403 17.908790877659662 40.58535080633068 30.129971642637248 5 12.281393275265122 38.90351146535354 36.10568814257417 12.709407116807172 6 29.18786652723209 38.35871924454117 19.782455412494492 12.670958815732256 7 27.22870918660096 31.867160967960178 23.192211329027863 17.711918516411 8 23.066891487480863 39.5109576358036 21.31759968256113 16.104551194154404 9 25.036420781588546 17.846230556155565 22.422022416741285 34.69532624551461 10-11 23.4477144395957 26.70360794821972 30.89013057354539 18.958547038639185 12-13 26.01122379310919 24.491372809302568 29.484390648900817 20.013012748687427 14-15 20.12043366204829 29.766402562349505 26.32802698231676 23.785136793285442 16-17 19.812262214386664 30.303935004730437 30.21912733021872 19.664675450664177 18-19 20.973574091251784 28.543131326372357 30.609798833640983 19.873495748734875 20-21 21.8368098004805 28.545759785362325 28.601117769770255 21.01631264438692 22-23 20.658157637251573 30.734798850417487 28.713252147616824 19.893791364714115 24-25 21.541476871019523 28.772108199457424 30.05925335256744 19.62716157695561 26-27 19.67659487815809 30.294263738794246 29.701159279900963 20.3279821031467 28-29 19.967280325112817 30.160860236745428 28.054944503349166 21.816914934792596 30-31 20.96281226478741 28.66051454404797 29.15437177839037 21.22230141277425 32-33 19.877223991873613 30.074698055709526 29.810352579580783 20.237725372836078 34-35 20.25179594266115 30.14369444400831 29.813766253168083 19.79074336016245 36-37 19.782383786999787 29.316218638184676 29.635323109334553 21.26607446548098 38-39 21.028850207106025 30.062988207374364 28.56029197524814 20.34786961027147 40-41 22.450307399960607 28.109992187730455 29.73465402874268 19.70504638356626 42-43 19.994081325630813 30.35433067119861 29.69007365990079 19.961514343269794 44-45 19.697466741300452 30.218475096684134 29.01544543451302 21.068612727502398 46-47 21.489902570246198 28.492350922199883 29.00138546461118 21.01636104294274 48-49 20.790572732748505 28.946559597453252 28.820454482738278 21.442413187059962 50-51 19.68726166500382 30.478856191845072 29.08185330720361 20.752028835947495 52-53 19.921025662496632 29.811563491104387 29.489462000112283 20.777948846286698 54-55 20.027243483759296 28.828920342546603 29.978132893580558 21.16570328011354 56-57 20.716954147401637 29.033738347015113 28.076259550047528 22.173047955535722 58-59 21.254202306379895 29.401966484316937 27.547052033902013 21.796779175401152 60-61 20.311210939841718 28.127691117516136 30.307823402742954 21.25327453989919 62-63 20.85786563175456 28.25835311379989 29.82701666792748 21.056764586518074 64-65 21.028313067573308 29.329091297498692 28.360478841918507 21.28211679300949 66-67 21.217067858267733 30.19523061420044 27.424039714114706 21.16366181341712 68-69 21.790264355169644 28.212673585298404 29.584846412322698 20.41221564720925 70-71 22.617115055413826 28.194276204169526 28.709020078769754 20.479588661646893 72-73 21.543284826212687 29.363333336505182 28.301231027175472 20.792150810106662 74-75 20.39285439014341 30.127789127718714 29.528570925606452 19.95078555653143 76-77 21.111992357107308 28.374857623528538 29.788226595782135 20.724923423582016 78-79 20.79522736074648 28.39266462208359 29.22358971821484 21.58851829895509 80-81 21.37041100530873 29.22145643161029 28.916435359416738 20.49169720366425 82-83 20.646524787129547 29.269035945982658 29.983373826277536 20.101065440610256 84-85 20.674001258908348 28.78342161392431 29.983642755496305 20.558934371671043 86-87 19.797648911583803 30.487857388395856 28.16698127907489 21.547512420945456 88-89 29.659598280095384 18.30096325979947 35.39204367218033 16.647394787924817 90-91 31.271750303770236 15.695741925325759 17.46990206434702 35.56260570655698 92-93 23.100153048488217 17.834535363452275 36.096035334341956 22.969276253717545 94-95 16.00477757124915 7.151588426187256 28.613587230072174 48.23004677249142 96-97 27.4375797297154 7.264572874631117 16.062353651165694 49.23549374448778 98-99 57.47669321479286 14.05884491641113 18.88861448965172 9.575847379144287 100-101 31.101297343539347 7.013688138439752 13.138722778403169 48.74629173961773 102-103 23.162434923084806 2.0490254127858982 38.026989193464836 36.76155047066446 104-105 40.75192369417452 4.340395636691407 42.95554413063793 11.952136538496132 106-107 5.864023434308855 1.8637744642280567 7.182268143370458 85.08993395809263 108-109 19.005149289662583 11.516265620496345 22.599499904602464 46.87908518523861 110-111 40.79784687780883 2.835714715214215 26.05229434957702 30.314144057399933 112-113 27.200500242732765 2.733235446819209 39.96903731539045 30.097226995057575 114-115 28.54155502750608 3.8654664667536323 25.048128882561915 42.544849623178365 116-117 19.927517205261 4.2281866811622795 21.311815107134596 54.532481006442126 118-119 20.650758412509816 4.265177757289405 16.82290693950193 58.26115689069885 120-121 25.700921538769546 4.216146038717975 31.103359157450267 38.979573265062214 122-123 34.3238473264991 1.6387578550502417 35.11277669436033 28.924618124090333 124-125 39.04568581997909 1.9821381180626831 20.420550390996194 38.55162567096203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 19.5 2 43.0 3 75.0 4 92.5 5 94.5 6 99.5 7 87.0 8 83.0 9 86.0 10 81.5 11 94.0 12 99.0 13 105.0 14 121.0 15 131.5 16 169.0 17 264.5 18 363.0 19 552.0 20 894.0 21 1454.5 22 2308.5 23 3545.0 24 5383.5 25 7882.0 26 11237.0 27 15840.0 28 21707.5 29 28987.0 30 38145.0 31 49481.0 32 63257.0 33 78132.5 34 92150.5 35 102491.5 36 107401.0 37 107655.0 38 105127.0 39 102012.0 40 98493.0 41 94075.0 42 90089.5 43 87303.5 44 84581.0 45 80886.5 46 76043.0 47 70985.5 48 66621.5 49 62203.5 50 56652.0 51 50467.0 52 43758.5 53 37210.0 54 31461.0 55 26208.5 56 21497.0 57 17411.5 58 14053.0 59 11168.0 60 8657.0 61 6617.5 62 4973.0 63 3623.5 64 2634.0 65 1873.0 66 1284.5 67 914.0 68 642.0 69 447.5 70 303.5 71 214.5 72 154.5 73 124.5 74 96.0 75 71.0 76 52.5 77 34.0 78 27.0 79 19.0 80 11.5 81 10.0 82 10.0 83 10.5 84 8.5 85 6.5 86 6.5 87 6.5 88 6.0 89 4.0 90 6.0 91 7.5 92 7.0 93 5.0 94 4.0 95 3.0 96 3.5 97 9.0 98 8.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 8.563998283394121E-4 3 0.048148701459971396 4 0.05390561141714188 5 0.05656996643864229 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.563998283394121E-4 12-13 0.001760377424919903 14-15 4.7577768241078455E-5 16-17 3.568332618080884E-4 18-19 0.007303187425005543 20-21 0.0022599439914512266 22-23 7.374554077367161E-4 24-25 0.0 26-27 0.0 28-29 0.0013559663948707358 30-31 0.0 32-33 0.0012845997425091182 34-35 4.7577768241078455E-5 36-37 1.1894442060269614E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.665221888437746E-4 46-47 0.0 48-49 1.9031107296431382E-4 50-51 4.7577768241078455E-5 52-53 0.0 54-55 0.001665221888437746 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.7577768241078455E-5 64-65 4.7577768241078455E-5 66-67 0.0 68-69 4.519887982902453E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.8062214592862764E-4 80-81 0.0 82-83 7.374554077367161E-4 84-85 4.7577768241078455E-5 86-87 0.0014035441631118143 88-89 19.31248221780912 90-91 26.26916075671489 92-93 40.34456771315554 94-95 44.74986464124935 96-97 48.40079226499675 98-99 93.06444599019326 100-101 51.67019376521894 102-103 53.11360809811677 104-105 74.87487046952597 106-107 81.65455971057493 108-109 12.597284641610946 110-111 39.073860678972814 112-113 52.02683671595406 114-115 86.81522507614822 116-117 66.47760847493271 118-119 71.84997112029467 120-121 80.39403431879579 122-123 75.85920691666563 124-125 49.98201560360487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2101822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.83146902249266 #Duplication Level Percentage of deduplicated Percentage of total 1 80.27305087772841 54.45038963953611 2 12.611818622389032 17.10956368403755 3 3.639942161679709 7.407078719509265 4 1.4887091659808764 4.039253187029308 5 0.6790271541011959 2.3029704684423304 6 0.3959147073130509 1.6113285722792678 7 0.22802114612700397 1.0826906516990997 8 0.1484291915086205 0.8054536084680496 9 0.1058313513805568 0.6460826429500897 >10 0.3887297310166104 4.477009221971431 >50 0.023039547777374735 1.0696566821544087 >100 0.01404729942326966 1.9470905530541414 >500 0.0020353523194653208 1.0329814581209533 >1k 0.0013335066920633768 1.7757493035260992 >5k 7.018456274017773E-5 0.2427016072218781 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5098 0.24255146249301798 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 4984 0.237127596913535 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 3997 0.19016833965959057 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 3317 0.15781545725565724 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2733 0.1300300406028674 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 2677 0.12736568558136704 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2157 0.10262524609600623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.7577768241078455E-5 4.7577768241078455E-5 12-13 0.0 0.0 0.0 7.136665236161768E-5 4.7577768241078455E-5 14-15 0.0 0.0 0.0 9.515553648215691E-5 9.515553648215691E-5 16-17 0.0 0.0 0.0 1.4273330472323536E-4 1.4273330472323536E-4 18-19 0.0 0.0 0.0 1.9031107296431382E-4 1.4273330472323536E-4 20-21 0.0 0.0 0.0 2.1409995708485303E-4 1.4273330472323536E-4 22-23 0.0 0.0 0.0 2.8546660944647073E-4 1.4273330472323536E-4 24-25 0.0 0.0 0.0 4.5198879829024533E-4 1.4273330472323536E-4 26-27 0.0 0.0 0.0 5.709332188929415E-4 1.4273330472323536E-4 28-29 0.0 0.0 0.0 9.991331330626476E-4 1.4273330472323536E-4 30-31 0.0 0.0 0.0 0.003021188283308482 1.4273330472323536E-4 32-33 0.0 0.0 0.0 0.00892083154520221 1.9031107296431382E-4 34-35 0.0 0.0 0.0 0.016747374420859617 2.3788884120539227E-4 36-37 0.0 0.0 0.0 0.029284116352383788 2.3788884120539227E-4 38-39 0.0 0.0 0.0 0.04993286776901184 2.3788884120539227E-4 40-41 0.0 0.0 0.0 0.07660020686813632 2.3788884120539227E-4 42-43 0.0 0.0 0.0 0.10319617931489916 2.3788884120539227E-4 44-45 0.0 0.0 0.0 0.13369352875743046 2.3788884120539227E-4 46-47 0.0 0.0 0.0 0.1704711436077841 3.0925549356701E-4 48-49 0.0 0.0 0.0 0.21243473519641531 3.330443776875492E-4 50-51 0.0 0.0 0.0 0.2556829265275556 3.330443776875492E-4 52-53 0.0 0.0 0.0 0.3005487619788926 3.568332618080884E-4 54-55 0.0 0.0 0.0 0.3542164845548291 3.8062214592862764E-4 56-57 0.0 0.0 0.0 0.4169239830965705 3.8062214592862764E-4 58-59 0.0 0.0 0.0 0.4874580245139693 4.0441103004916685E-4 60-61 0.0 0.0 0.0 0.5618696540430159 4.281999141697061E-4 62-63 0.0 0.0 0.0 0.6377799832716566 4.7577768241078455E-4 64-65 0.0 0.0 0.0 0.7178771561055122 4.7577768241078455E-4 66-67 0.0 0.0 0.0 0.8132467925447541 4.7577768241078455E-4 68-69 0.0 0.0 0.0 0.9288131916023336 4.995665665313238E-4 70-71 0.0 0.0 0.0 1.0571066436644017 5.23355450651863E-4 72-73 0.0 0.0 0.0 1.180785052207085 6.660887553750984E-4 74-75 0.0 0.0 0.0 1.306866138045943 6.660887553750984E-4 76-77 0.0 0.0 0.0 1.4515025534988215 6.660887553750984E-4 78-79 0.0 0.0 0.0 1.4930141562891626 6.660887553750984E-4 80-81 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 82-83 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 84-85 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 86-87 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 88-89 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 90-91 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 92-93 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 94-95 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 96-97 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 98-99 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 100-101 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 102-103 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 104-105 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 106-107 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 108-109 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 110-111 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 112-113 0.0 0.0 0.0 1.4937516116968992 6.660887553750984E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 430 0.0 2749.0613 108-109 CGTCGCG 40 1.8189894E-12 2462.7004 108-109 CCGCGCC 390 0.0 2083.8235 108-109 CGCGTCT 50 4.800313E-9 1836.9734 110-111 CCGCTCG 145 0.0 1698.414 108-109 CGTCGTC 70 1.843182E-8 1312.1238 110-111 CGACGCC 60 2.3810571E-8 1231.3502 108-109 CTCCGCC 255 0.0 1200.6362 110-111 CGTCGCC 195 0.0 1136.631 108-109 CCGCGCA 65 3.2789103E-8 1136.631 108-109 CCGCGAC 45 1.6568927E-5 1094.5336 108-109 CCGCGCG 115 0.0 1070.7394 108-109 TTGTTCG 315 0.0 938.1716 108-109 CTTCGCT 175 0.0 874.7492 110-111 CAGCTCG 155 1.8189894E-12 794.4195 108-109 CTCCGCT 215 1.8189894E-12 712.00525 110-111 CCGTTCG 70 6.231919E-5 703.62866 108-109 CCGCACG 105 2.2298445E-7 703.62866 108-109 CGTCGTT 90 6.886045E-5 680.3606 110-111 AAGCGCG 110 2.685465E-7 671.64557 108-109 >>END_MODULE