FastQCFastQC Report
Fri 27 May 2016
SRR939923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101822
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52260.248641416827876No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA49020.2332262199177666No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC39410.1875039846380902No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA31550.1501078588006025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.14059230515238685No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA27330.1300300406028674No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26660.12684233013071516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.10410015691147967No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21810.1037671125337921No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1503.6670826E-935.7517621
TAGGACC33600.031.7423634
GTACATG63550.029.1602381
TACATGG62000.028.3501832
ACATGGG60650.026.7216723
CATGGGG32750.025.4707814
GTCCTAC110500.024.9669191
CTACAGT114300.024.8655284
TACAGTG117150.024.6674925
TCCGTAC1451.3279004E-424.6552143
AGAGTAC81500.024.41814410-11
GAGTACT51950.024.33590912-13
TCCTACA121500.023.8824142
CCTACAG118050.023.5204413
AGTACTT53250.023.46247712-13
GTACTTT53350.023.41961314-15
GTAGGAC114550.023.2508053
TGTAGGA121900.022.5820732
AGTGGAC74600.022.489748
TACACCG2153.3831875E-622.1705825
Produced by FastQC (version 0.11.5)