FastQCFastQC Report
Fri 27 May 2016
SRR939922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766147
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15720.20518255635015212No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA14280.18638720767685574No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC12830.1674613357488837No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9020.11773197571745371No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA8690.11342470831315662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT52.2085999E-46075.8237110-111
CGTGTCG108.833439E-43037.9119110-111
CAGCGCG304.688738E-61663.3436108-109
CGACGCG150.00294848461663.3436108-109
CGTCGAC150.00294848461663.3436108-109
CCACGCG150.00294848461663.3436108-109
CCGCACG150.00294848461663.3436108-109
CCGCTCC1900.01444.4825108-109
CCGCGCC1600.01403.446108-109
CTCCGCT458.74278E-61350.1831110-111
CCGTGCG200.00524105551247.5077108-109
CGCGTCG200.00524105551247.5077108-109
CCGTTCG200.00524105551247.5077108-109
AATCGCG200.00524105551247.5077108-109
CGTCGCG250.00551909951215.1648110-111
CGCCGCC1250.01215.1648110-111
CCGCGCG1050.01188.1024108-109
TTGTTCG1100.01134.0978108-109
CCGCTCG704.1247404E-81069.2922108-109
CGTTGCG300.0079466391012.6374110-111
Produced by FastQC (version 0.11.5)