##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939922_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 766147 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76140610091797 33.0 31.0 34.0 30.0 34.0 2 31.923023910555024 34.0 31.0 34.0 30.0 34.0 3 32.04824792109086 34.0 31.0 34.0 30.0 34.0 4 35.25068687862773 37.0 35.0 37.0 33.0 37.0 5 35.28956975619561 37.0 35.0 37.0 33.0 37.0 6 35.364764203214264 37.0 37.0 37.0 33.0 37.0 7 35.29580876776911 37.0 37.0 37.0 33.0 37.0 8 35.27642998014741 37.0 37.0 37.0 33.0 37.0 9 36.90677768104555 39.0 39.0 39.0 34.0 39.0 10-11 36.88641866378123 39.0 39.0 39.0 34.0 39.0 12-13 36.82013699720811 39.0 38.0 39.0 34.0 39.0 14-15 38.34172097521755 41.0 39.0 41.0 34.0 41.0 16-17 38.35019976584128 41.0 39.0 41.0 34.0 41.0 18-19 38.37556891823632 41.0 39.0 41.0 34.0 41.0 20-21 38.437517212754216 41.0 39.0 41.0 34.0 41.0 22-23 38.43939022145881 41.0 39.0 41.0 34.0 41.0 24-25 38.475638487131064 41.0 39.0 41.0 34.0 41.0 26-27 38.446991243194844 41.0 39.0 41.0 34.0 41.0 28-29 38.3974361317084 41.0 39.0 41.0 33.0 41.0 30-31 38.3236304521195 41.0 39.0 41.0 33.0 41.0 32-33 38.23041465932778 41.0 39.0 41.0 33.0 41.0 34-35 38.169911909855415 40.0 38.0 41.0 33.0 41.0 36-37 38.33945770198147 41.0 39.0 41.0 33.0 41.0 38-39 38.291844123908334 41.0 39.0 41.0 33.0 41.0 40-41 38.212914101340864 41.0 39.0 41.0 33.0 41.0 42-43 38.159766337269474 41.0 39.0 41.0 33.0 41.0 44-45 38.08037034668281 41.0 38.5 41.0 33.0 41.0 46-47 37.97649080398409 41.0 38.0 41.0 33.0 41.0 48-49 37.89194110268656 40.5 38.0 41.0 33.0 41.0 50-51 37.7842528914164 40.0 38.0 41.0 32.0 41.0 52-53 37.6978001610657 40.0 38.0 41.0 32.0 41.0 54-55 37.559487931167254 40.0 38.0 41.0 32.0 41.0 56-57 37.36927443427959 40.0 37.5 41.0 31.0 41.0 58-59 37.2046885258312 40.0 37.0 41.0 31.0 41.0 60-61 36.99715850874571 40.0 37.0 41.0 31.0 41.0 62-63 36.756089236138756 40.0 36.0 41.0 31.0 41.0 64-65 36.47469676184858 39.5 36.0 41.0 30.0 41.0 66-67 36.16195064393648 39.0 35.0 41.0 30.0 41.0 68-69 35.7550437448688 39.0 35.0 41.0 29.5 41.0 70-71 35.332877372097 38.0 35.0 40.0 29.0 41.0 72-73 34.93450016772238 37.0 35.0 40.0 29.0 41.0 74-75 34.50954320776562 37.0 34.5 39.0 28.0 41.0 76-77 34.02555123233531 36.0 34.0 39.0 28.0 40.5 78-79 33.54823552138167 36.0 34.0 38.5 26.5 40.0 80-81 33.09156597885262 35.0 34.0 37.0 26.0 39.0 82-83 32.86631873517745 35.0 34.0 37.0 26.0 39.0 84-85 32.40681944848704 35.0 34.0 36.5 26.0 39.0 86-87 31.7326009238436 35.0 34.0 36.0 13.0 37.0 88-89 16.233326633139594 18.0 16.5 18.5 2.0 19.5 90-91 2.7851052082694316 2.0 2.0 2.0 2.0 2.0 92-93 2.3037360976418366 2.0 2.0 2.0 2.0 2.0 94-95 2.103053330496628 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11276759466381137 1101 2 0.16416540463050922 1101 3 0.18624922925371123 1101 4 0.31961558390690925 1101 5 0.18002787899490613 1101 6 0.23634131527560243 1101 7 0.11701988133894048 1101 8 0.04430536286776032 1101 9 0.17089614962333144 1101 10-11 0.16558024507463642 1101 12-13 0.13856474266072638 1101 14-15 0.05219135840741984 1101 16-17 0.2991576402821323 1101 18-19 0.24190393239410923 1101 20-21 0.2770945308315689 1101 22-23 0.22333637423970032 1101 24-25 0.28331846117724524 1101 26-27 0.28161935231916146 1101 28-29 0.361515300929689 1101 30-31 0.281483099827895 1101 32-33 0.19585792628153342 1101 34-35 0.306684057570628 1101 36-37 0.2745962016205823 1101 38-39 0.298026142500845 1101 40-41 0.38090507116605465 1101 42-43 0.37627398900652054 1101 44-45 0.40770559353518365 1101 46-47 0.42866095005918226 1101 48-49 0.5103512529149583 1101 50-51 0.3083199546355644 1101 52-53 0.2301609668454816 1101 54-55 0.2983723963328515 1101 56-57 0.19290999099361272 1101 58-59 0.18681137264215408 1101 60-61 0.20822136485004705 1101 62-63 0.2672927539845489 1101 64-65 0.20599273323870193 1101 66-67 0.35846003117551106 1101 68-69 0.23131381600793333 1101 70-71 0.22742807815466648 1101 72-73 0.050810539628962204 1101 74-75 0.11257030576141602 1101 76-77 0.10283874333692467 1101 78-79 -0.02354672271358993 1101 80-81 0.11295894435140497 1101 82-83 0.14237161740457083 1101 84-85 0.28838756440540436 1101 86-87 0.4587373815094331 1101 88-89 0.7636280206575776 1101 90-91 -0.13499297485574902 1101 92-93 -0.22844259933624889 1101 94-95 -0.025301364242874325 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.048953486607032914 1102 2 0.161264755165881 1102 3 0.11784480587298418 1102 4 -0.03695805133970964 1102 5 0.15441790883517825 1102 6 0.07038116080833845 1102 7 0.07698565279760317 1102 8 0.11193306165660033 1102 9 0.06087333059576849 1102 10-11 0.07247422436198292 1102 12-13 0.09346425117397672 1102 14-15 0.16414940650290788 1102 16-17 0.09621969048399137 1102 18-19 0.046171529374973375 1102 20-21 -0.03173248338643475 1102 22-23 0.09212696577910862 1102 24-25 0.09824078871805852 1102 26-27 0.042515437978281057 1102 28-29 -0.0031261770411745715 1102 30-31 0.025852943605315204 1102 32-33 0.19840970792098744 1102 34-35 0.040293852199383196 1102 36-37 -3.5552735957367076E-4 1102 38-39 0.12576223167057776 1102 40-41 0.16695255229647898 1102 42-43 0.1844932272020543 1102 44-45 0.10136892511321349 1102 46-47 0.1515041131274657 1102 48-49 -0.02746689698849991 1102 50-51 0.002270718195333643 1102 52-53 0.09654678394219474 1102 54-55 0.025068464438874116 1102 56-57 -0.040127133808908866 1102 58-59 -0.09284897490918809 1102 60-61 -0.10415356932583819 1102 62-63 -0.07994482719853124 1102 64-65 0.008608144858854416 1102 66-67 0.06875053494942307 1102 68-69 0.003880517780800119 1102 70-71 -0.055343556038764063 1102 72-73 0.16105496652030382 1102 74-75 0.11718457643018354 1102 76-77 0.1063405864122231 1102 78-79 0.13452989655970526 1102 80-81 0.15221644830084813 1102 82-83 0.13756206851919472 1102 84-85 0.20049920210946226 1102 86-87 0.15484059372638725 1102 88-89 0.7053627055794642 1102 90-91 0.17344567706631464 1102 92-93 0.1114928930281538 1102 94-95 0.1879797486235466 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.11000047542410485 1103 2 0.05300323880187108 1103 3 0.12015320845839739 1103 4 0.03910637874133727 1103 5 0.011922782129524023 1103 6 0.04514519076627721 1103 7 0.07411553891640921 1103 8 0.18196230142267922 1103 9 0.1117607831452716 1103 10-11 0.087389069955492 1103 12-13 0.08634539408974007 1103 14-15 0.1064521663264486 1103 16-17 0.20579314898404988 1103 18-19 0.20059468521779422 1103 20-21 0.09731876315095889 1103 22-23 0.1857455150763272 1103 24-25 0.0989140728054636 1103 26-27 0.1352850081024215 1103 28-29 0.057609177426868996 1103 30-31 0.206275124789272 1103 32-33 0.16068399744597173 1103 34-35 0.16058471092514282 1103 36-37 0.13898991226287194 1103 38-39 0.13882907274988554 1103 40-41 0.12842403070008146 1103 42-43 0.100357082747351 1103 44-45 0.13819051124227144 1103 46-47 -0.028983472796412002 1103 48-49 0.013504684010783308 1103 50-51 0.04248693857083197 1103 52-53 -0.03268272257262339 1103 54-55 -0.09759107182868831 1103 56-57 0.01614017921047406 1103 58-59 -0.010138910273909119 1103 60-61 -0.10901660410576852 1103 62-63 -0.2781352960607464 1103 64-65 -0.1262846426655173 1103 66-67 -0.10419242185779609 1103 68-69 -0.056500881624145904 1103 70-71 0.03843401654767575 1103 72-73 0.03599212667214857 1103 74-75 0.08810255119165333 1103 76-77 0.15711005394069844 1103 78-79 -0.12049087906355993 1103 80-81 -0.08633387487550692 1103 82-83 -0.05974992491468356 1103 84-85 -0.10473317708414243 1103 86-87 -0.008902096332089826 1103 88-89 0.6076005736414345 1103 90-91 -0.577608493447689 1103 92-93 -0.32322932919406844 1103 94-95 -0.11155728841349033 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.06663155281521682 1104 2 0.21749777709657536 1104 3 0.27765398893798476 1104 4 0.17066137488436794 1104 5 0.31475051335382886 1104 6 0.17158536143114844 1104 7 0.3756986323245357 1104 8 0.3349453852503146 1104 9 0.33046850716768716 1104 10-11 0.26696007873155736 1104 12-13 0.3780589308267892 1104 14-15 0.2868470146656321 1104 16-17 0.3248679048880234 1104 18-19 0.3852086657958296 1104 20-21 0.3495957813720594 1104 22-23 0.26707561747194575 1104 24-25 0.25125460306907854 1104 26-27 0.26444116513656724 1104 28-29 0.35242026833085305 1104 30-31 0.32194550306996916 1104 32-33 0.32480687879490233 1104 34-35 0.30667727497814923 1104 36-37 0.20152945243238207 1104 38-39 0.2115561427551924 1104 40-41 0.35520455653684735 1104 42-43 0.4428298733294014 1104 44-45 0.2964659899777189 1104 46-47 0.12652316169302225 1104 48-49 0.1918665688565966 1104 50-51 0.1623954448898246 1104 52-53 0.2409084085363773 1104 54-55 0.10708082116053674 1104 56-57 0.09944501884463364 1104 58-59 0.1483108342738788 1104 60-61 0.14088717804049367 1104 62-63 0.19813193023869502 1104 64-65 0.2115366547641102 1104 66-67 0.342813014244463 1104 68-69 0.25404864207682465 1104 70-71 0.2256111911950498 1104 72-73 0.36533758097733937 1104 74-75 0.34770286305312226 1104 76-77 0.46631872197175994 1104 78-79 0.4277245894637076 1104 80-81 0.23605663911778407 1104 82-83 0.3720051878460353 1104 84-85 0.4159036942581338 1104 86-87 0.5552083138615522 1104 88-89 0.7376218654549049 1104 90-91 -0.6441999202304451 1104 92-93 -0.32322932919406844 1104 94-95 -0.11155728841349033 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.058846274131397536 1105 2 -0.09482686276784236 1105 3 -0.08215519412701511 1105 4 -0.018049669273437985 1105 5 0.06192278212952118 1105 6 0.07247667737754426 1105 7 0.06894471712508476 1105 8 -0.14786225998082614 1105 9 0.025103350088947707 1105 10-11 -0.03010862164191508 1105 12-13 -0.03653087553167467 1105 14-15 -0.10972973579728063 1105 16-17 -0.03504249275187732 1105 18-19 -0.033823597330680855 1105 20-21 0.0930482183679473 1105 22-23 -0.11289252739828015 1105 24-25 0.03647178287010178 1105 26-27 0.04373376156502928 1105 28-29 0.1083016982024958 1105 30-31 -0.05051157881183599 1105 32-33 -0.14250159812189622 1105 34-35 -0.034267551309390853 1105 36-37 -0.11595006926991402 1105 38-39 -0.07552919133136982 1105 40-41 0.01999836126333321 1105 42-43 0.11967841238724475 1105 44-45 -0.015249008609984571 1105 46-47 -0.07205826504017665 1105 48-49 -0.07532264747582929 1105 50-51 -0.03669127010876139 1105 52-53 -0.0952173486114063 1105 54-55 -0.050638163241430334 1105 56-57 -0.025872747844005062 1105 58-59 -0.1459191503477797 1105 60-61 -0.178130177512962 1105 62-63 -0.18723040224725906 1105 64-65 -0.1508460461742871 1105 66-67 -0.06716564438780637 1105 68-69 -0.12406782529912164 1105 70-71 -0.10821879970348647 1105 72-73 -0.15022670989294795 1105 74-75 -0.2616896925756649 1105 76-77 -0.18035532002051724 1105 78-79 -0.04989992800169318 1105 80-81 -0.16664320082195871 1105 82-83 -0.13680440321569876 1105 84-85 -0.02742477449872638 1105 86-87 0.029694394895980025 1105 88-89 0.6711278127919407 1105 90-91 -0.7904432118225739 1105 92-93 -0.32322932919406844 1105 94-95 -0.11155728841349033 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.2427418326286812 1106 2 -0.21255635562400954 1106 3 -0.1537654861189175 1106 4 -0.04748774842621373 1106 5 -0.02873393337998209 1106 6 -0.12682339413689192 1106 7 -0.19879010127390728 1106 8 -0.16576521337471917 1106 9 -0.1327921475449898 1106 10-11 -0.2785946458959643 1106 12-13 -0.37655879659191527 1106 14-15 -0.29869711304352364 1106 16-17 -0.2540931006245515 1106 18-19 -0.3091218277288732 1106 20-21 -0.28248053195539313 1106 22-23 -0.2792552577280034 1106 24-25 -0.3033986542808833 1106 26-27 -0.47113895796145755 1106 28-29 -0.34508154307502537 1106 30-31 -0.15006575479819872 1106 32-33 -0.21487648590509423 1106 34-35 -0.24126873814464744 1106 36-37 -0.12827946170155258 1106 38-39 -0.05664557726584718 1106 40-41 0.005771173606419211 1106 42-43 -0.369053880182868 1106 44-45 -0.11601246321353642 1106 46-47 -0.30720304372583485 1106 48-49 -0.05213523401590692 1106 50-51 -0.1607954377264207 1106 52-53 -0.12136462269427284 1106 54-55 -0.18896457336909123 1106 56-57 -0.34572484991183217 1106 58-59 -0.20893021226102348 1106 60-61 -0.02160562676054667 1106 62-63 -0.06136728391370383 1106 64-65 0.018007136217953246 1106 66-67 -0.06299194039669231 1106 68-69 -0.10993649741305944 1106 70-71 0.024581598951435524 1106 72-73 -0.0842017711366907 1106 74-75 -0.3728980582426473 1106 76-77 -0.1920741770068659 1106 78-79 -0.14363937201502353 1106 80-81 -0.09122096135511981 1106 82-83 -0.1643427851636261 1106 84-85 0.04413416404450743 1106 86-87 -0.023775576650404417 1106 88-89 0.7652134170888942 1106 90-91 -0.20935041043488978 1106 92-93 -0.30891883483153615 1106 94-95 -0.11155728841349033 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.038574684533458026 1107 2 0.04260102398106724 1107 3 0.019474935159102813 1107 4 0.0996480319780062 1107 5 0.003727758313658569 1107 6 0.0197800115984208 1107 7 -0.09684327731294928 1107 8 0.013343029803500883 1107 9 0.02539772007799712 1107 10-11 0.05426018401472277 1107 12-13 0.053208333367471994 1107 14-15 0.01953788942090995 1107 16-17 0.06876434728244618 1107 18-19 -0.04869371951640744 1107 20-21 -0.07323754240835711 1107 22-23 -0.0018240678852450287 1107 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2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 -0.11629251172100297 2216 2 -0.22558547817015295 2216 3 -0.2236215694608319 2216 4 -0.1504106153385223 2216 5 -0.18217133882472325 2216 6 -0.26170254343486477 2216 7 -0.2742504886606838 2216 8 -0.2829180348878708 2216 9 -0.3995404699491303 2216 10-11 -0.3354490849633649 2216 12-13 -0.4274021268573662 2216 14-15 -0.4218983127385414 2216 16-17 -0.4606532695116883 2216 18-19 -0.35953340170323145 2216 20-21 -0.3590176254112052 2216 22-23 -0.3491343975507206 2216 24-25 -0.40245213836779925 2216 26-27 -0.5525204391224179 2216 28-29 -0.42811235221853394 2216 30-31 -0.4946893940710311 2216 32-33 -0.5146480056556584 2216 34-35 -0.4511907314374213 2216 36-37 -0.575263137081464 2216 38-39 -0.5488294302754184 2216 40-41 -0.49858081610470606 2216 42-43 -0.5448093273554164 2216 44-45 -0.6129576374289343 2216 46-47 -0.7587023150778762 2216 48-49 -0.660494061079028 2216 50-51 -0.6702539641709393 2216 52-53 -0.7001217709929293 2216 54-55 -0.773353819826454 2216 56-57 -0.8407613896962687 2216 58-59 -0.8277421528649995 2216 60-61 -0.8737902125240211 2216 62-63 -0.8895105928913907 2216 64-65 -0.8553259275967022 2216 66-67 -0.8069683558277276 2216 68-69 -0.8229379521822437 2216 70-71 -0.8788673347336982 2216 72-73 -0.8223913257296971 2216 74-75 -0.8991492829207814 2216 76-77 -0.9726551245208483 2216 78-79 -0.9748261364580131 2216 80-81 -0.9577738406042258 2216 82-83 -0.9953207779106279 2216 84-85 -0.9184698033125684 2216 86-87 -1.0284967858964968 2216 88-89 -1.6602070794276838 2216 90-91 -0.7904432118225739 2216 92-93 -0.32322932919406844 2216 94-95 -0.11155728841349033 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8779.0 3 1509.0 4 1066.0 5 835.0 6 825.0 7 951.0 8 1065.0 9 1317.0 10 1510.0 11 1988.0 12 2417.0 13 2983.0 14 3447.0 15 3640.0 16 4108.0 17 4713.0 18 6005.0 19 7374.0 20 7744.0 21 10843.0 22 12419.0 23 15150.0 24 23467.0 25 38925.0 26 70241.0 27 199553.0 28 320858.0 29 11938.0 30 477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57335341651145 20.445032089142163 14.971669927572645 23.009944566773736 2 12.850342560070379 20.555170509943967 45.12121105328901 21.473275876696647 3 19.173499328159707 26.689231098697192 28.915640624857176 25.221628948285925 4 11.289204157400357 17.65014684623391 40.120950628585454 30.939698367780277 5 12.274974044884393 39.36871935174242 34.75438951609217 13.601917087281015 6 28.81013695805113 37.49567641718887 20.594611739000477 13.099574885759521 7 27.996585511657685 32.05455349952424 22.377820444379473 17.5710405444386 8 24.2021439749813 38.61099762839246 20.897686736357382 16.289171660268853 9 25.853654716392544 18.450636757697936 22.060518412262923 33.635190113646594 10-11 23.702655643470795 27.352109661133316 29.89772131014877 19.04751338524712 12-13 25.963592364486203 24.98118793077681 29.217868235241905 19.83735146949508 14-15 20.528371187252578 29.615008608008647 26.396435670961317 23.46018453377746 16-17 19.98634719628059 30.801364758276193 29.627040904896347 19.58524714054687 18-19 20.856413178338588 29.001534414015058 30.565905725237425 19.576146682408933 20-21 22.17543594017476 28.72248432547154 28.52937126328835 20.572708471065347 22-23 20.798883239388914 30.574845867848687 28.71892965360973 19.907341239152668 24-25 21.33454806975685 28.975770968234553 30.17279973686512 19.51688122514348 26-27 19.753585147497805 30.39853970582669 29.66493375292209 20.182941393753417 28-29 19.779557117209073 30.53406070304429 28.140044991806324 21.546337187940317 30-31 20.75123964461128 29.02732765383145 29.361858755565184 20.85957394599209 32-33 19.747682532389728 30.163952186159676 29.97717121458536 20.111194066865238 34-35 20.188645506209 30.36496960109431 29.84633477170148 19.600050120995217 36-37 19.670115526132715 29.67087256100983 29.655340293703425 21.003671619154026 38-39 20.673643569706595 30.459820373896918 28.694297569526473 20.172238486870015 40-41 22.225238759663615 28.537604402288334 29.732022705825383 19.50513413222267 42-43 19.812646920238546 30.481226187663722 29.78162154260214 19.924505349495593 44-45 19.685647793439117 30.477310490023456 29.116475036774926 20.720566679762502 46-47 21.092362170706146 29.018321549258825 29.00109247964164 20.888223800393398 48-49 20.498285577049835 29.497733463682557 28.97276893337701 21.031212025890593 50-51 19.50767933568884 30.779341301342956 29.203925617407627 20.509053745560575 52-53 19.636114218289702 30.050238400724666 29.70781064208305 20.605836738902585 54-55 19.73584255495775 29.22713259038236 30.0504872515774 20.98653760308249 56-57 20.430805054382514 29.519530847213392 28.275383183644916 21.774280914759178 58-59 20.882284992305657 29.922325611142508 27.869651646485593 21.32573775006624 60-61 20.16571232413623 28.652725912912274 30.43867560663946 20.74288615631204 62-63 20.47472612958088 28.72386108671051 29.959328953843062 20.842083829865548 64-65 20.75922865876739 29.70634839834705 28.481516577780212 21.052906365105343 66-67 20.735120022658837 30.668527058123313 27.759816327676017 20.836536591541833 68-69 21.42066075089066 28.700302227976877 29.714015716376892 20.16502130475557 70-71 22.088711435272863 28.72333899369181 28.93981181157141 20.248137759463916 72-73 21.091709554432768 29.806943054009217 28.714202365864516 20.387145025693503 74-75 20.28331377659901 30.37445816533903 29.566453957269296 19.775774100792667 76-77 20.811476126644106 28.941051782490828 29.870964710427632 20.376507380437438 78-79 20.474414731434297 28.98449899757748 29.34663718569877 21.19444908528945 80-81 20.963339933459242 29.656580264622846 29.117062391420966 20.263017410496943 82-83 20.478788839920565 29.61561278171199 30.053978279469263 19.851620098898177 84-85 20.358508457586115 29.42740063421291 30.008360019917863 20.205730888283117 86-87 19.488289953363434 30.907983820126166 28.455485115586377 21.148241110924026 88-89 29.538207682101223 18.617144205455077 35.36192370907762 16.48272440336607 90-91 30.936480764990254 15.746929066778131 17.28808546098757 36.028504707244046 92-93 22.603751074041845 18.005702683325943 36.17734141122257 23.213204831409637 94-95 15.60870500010632 7.141388915797009 28.322437513437652 48.927468570659016 96-97 26.724327995479 7.205976639812672 15.769140123111075 50.30055524159726 98-99 57.08630245447348 14.12264826754138 18.84779248199676 9.943256795988388 100-101 30.847623965545672 6.988599035906143 12.998910460391317 49.164866538156865 102-103 22.878104812965425 2.041530402005147 37.93050571963075 37.14985906539867 104-105 40.36229869768867 4.314279702076476 43.011864464020825 12.311557136214022 106-107 5.691936955001828 1.8554337449027032 6.998335504157691 85.45429379593777 108-109 18.19220063403532 11.64358765161349 22.314251313566817 47.84996040078437 110-111 40.265341707509336 2.794367138499503 25.88307999451623 31.057211159474928 112-113 26.86724675572259 2.719610005855359 39.79247518280975 30.620668055612292 114-115 28.141476491477974 3.8994569249488085 24.786578429335947 43.17248815423727 116-117 19.509588449087097 4.194544969641282 20.931260232318653 55.364606348952975 118-119 20.210373560745772 4.180261722756205 16.43337046537534 59.17599425112269 120-121 25.115364072758155 4.212488845098644 30.68833620292134 39.983810879221856 122-123 33.872598311114 1.6648285705018 34.63486760301448 29.82770551536973 124-125 38.63779308252665 2.0380276662321073 20.26520773210907 39.05897151913217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 18.0 2 47.0 3 72.0 4 87.5 5 92.5 6 87.0 7 77.0 8 71.0 9 65.5 10 57.5 11 54.0 12 61.5 13 79.5 14 93.0 15 115.0 16 162.0 17 240.0 18 324.0 19 467.5 20 690.5 21 1038.0 22 1515.0 23 2214.0 24 3179.0 25 4250.5 26 5703.5 27 7502.0 28 9645.0 29 12411.5 30 15730.0 31 19680.5 32 24367.5 33 29297.0 34 33997.5 35 37412.5 36 38859.0 37 38686.5 38 37414.5 39 35921.5 40 34456.5 41 32583.0 42 30794.5 43 29383.5 44 28007.0 45 26674.5 46 25235.0 47 23811.0 48 22567.0 49 21255.0 50 19740.0 51 17861.5 52 15710.5 53 13643.5 54 11756.5 55 10142.0 56 8449.5 57 6921.0 58 5737.5 59 4658.5 60 3739.0 61 2869.0 62 2138.5 63 1606.5 64 1208.5 65 894.5 66 635.5 67 480.0 68 367.0 69 279.5 70 208.0 71 145.5 72 100.0 73 70.0 74 52.0 75 43.0 76 36.5 77 26.0 78 15.0 79 8.5 80 7.5 81 6.5 82 6.0 83 4.0 84 2.0 85 2.0 86 2.0 87 2.0 88 2.0 89 2.5 90 2.0 91 2.0 92 1.5 93 1.0 94 1.5 95 1.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 5.220930187026772E-4 3 0.04437790658972756 4 0.049598836776754335 5 0.05247034837961906 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.526162733783464E-4 12-13 0.002153633702148543 14-15 0.0 16-17 5.220930187026772E-4 18-19 0.007374563889175315 20-21 0.002023110447472874 22-23 3.263081366891732E-4 24-25 0.0 26-27 0.0 28-29 9.789244100675196E-4 30-31 0.0 32-33 0.0011747092920810236 34-35 1.305232546756693E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.001696802310783701 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.305232546756693E-4 66-67 0.0 68-69 4.568313913648425E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.9156976402700787E-4 80-81 0.0 82-83 7.178779007161811E-4 84-85 6.526162733783465E-5 86-87 0.0013052325467566928 88-89 19.237039367118843 90-91 26.305460962452376 92-93 40.37665095601758 94-95 44.7559019352683 96-97 48.49513213521687 98-99 93.07626343247445 100-101 51.721928037308764 102-103 53.18431058269497 104-105 74.87877652721997 106-107 81.61142704990034 108-109 12.570759919441047 110-111 39.067567973247954 112-113 52.073818731914365 114-115 86.80527366158192 116-117 66.47568939120039 118-119 71.93769602961312 120-121 80.32773084016513 122-123 75.85659148962274 124-125 50.09058313874492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 766147.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.41012661683389 #Duplication Level Percentage of deduplicated Percentage of total 1 89.45285799656668 71.92915637741591 2 7.728263494700989 12.428612922743234 3 1.4625389746581219 3.528088324029419 4 0.5019400334673161 1.614442465806589 5 0.2407835808443194 0.968071911147319 6 0.13865982882133038 0.6689792635315014 7 0.0904132593674785 0.5089099143505713 8 0.059279775258927005 0.3813355387510222 9 0.04302490937142049 0.3113674569210353 >10 0.250894394829063 3.626925069440717 >50 0.016890747719352832 0.8948210379182596 >100 0.012829071346820263 1.9575347266942484 >500 0.001136753133684154 0.6224780158331699 >1k 4.87179914436066E-4 0.5592769754171316 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1572 0.20518255635015212 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1428 0.18638720767685574 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1283 0.1674613357488837 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 902 0.11773197571745371 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 869 0.11342470831315662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.610465093513386E-4 0.0 10-11 0.0 0.0 0.0 2.610465093513386E-4 0.0 12-13 0.0 0.0 0.0 2.610465093513386E-4 0.0 14-15 0.0 0.0 0.0 2.610465093513386E-4 0.0 16-17 0.0 0.0 0.0 2.610465093513386E-4 0.0 18-19 0.0 0.0 0.0 2.610465093513386E-4 0.0 20-21 0.0 0.0 0.0 2.610465093513386E-4 0.0 22-23 0.0 0.0 0.0 2.610465093513386E-4 0.0 24-25 0.0 0.0 0.0 3.263081366891732E-4 0.0 26-27 0.0 0.0 0.0 3.9156976402700787E-4 0.0 28-29 0.0 0.0 0.0 0.0013052325467566928 0.0 30-31 0.0 0.0 0.0 0.0031978197395538975 0.0 32-33 0.0 0.0 0.0 0.006787209243134803 0.0 34-35 0.0 0.0 0.0 0.012987063840229095 1.305232546756693E-4 36-37 0.0 0.0 0.0 0.02049215098408008 1.305232546756693E-4 38-39 0.0 0.0 0.0 0.03563284852645772 1.305232546756693E-4 40-41 0.0 0.0 0.0 0.05579869137384862 1.305232546756693E-4 42-43 0.0 0.0 0.0 0.07426773191045583 1.305232546756693E-4 44-45 0.0 0.0 0.0 0.0966524700873331 1.305232546756693E-4 46-47 0.0 0.0 0.0 0.12406235356922365 1.305232546756693E-4 48-49 0.0 0.0 0.0 0.15205959169715472 1.305232546756693E-4 50-51 0.0 0.0 0.0 0.18305886468262617 1.305232546756693E-4 52-53 0.0 0.0 0.0 0.21673386438894887 1.305232546756693E-4 54-55 0.0 0.0 0.0 0.2591539221585414 1.305232546756693E-4 56-57 0.0 0.0 0.0 0.3092748519539984 1.305232546756693E-4 58-59 0.0 0.0 0.0 0.3610925840602391 1.305232546756693E-4 60-61 0.0 0.0 0.0 0.4213290660930605 1.305232546756693E-4 62-63 0.0 0.0 0.0 0.48574229227550325 1.305232546756693E-4 64-65 0.0 0.0 0.0 0.5514607510047027 1.305232546756693E-4 66-67 0.0 0.0 0.0 0.6257284829151586 1.305232546756693E-4 68-69 0.0 0.0 0.0 0.7179431623435188 1.305232546756693E-4 70-71 0.0 0.0 0.0 0.819359731226514 1.305232546756693E-4 72-73 0.0 0.0 0.0 0.9181658350159956 1.305232546756693E-4 74-75 0.0 0.0 0.0 1.0180161248428825 1.305232546756693E-4 76-77 0.0 0.0 0.0 1.1300050773546069 1.305232546756693E-4 78-79 0.0 0.0 0.0 1.163092722414889 1.305232546756693E-4 80-81 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 82-83 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 84-85 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 86-87 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 88-89 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 90-91 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 92-93 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 94-95 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 96-97 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 98-99 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 100-101 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 102-103 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 104-105 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 106-107 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 108-109 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 110-111 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 112-113 0.0 0.0 0.0 1.163875861942943 1.305232546756693E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGACT 5 2.2085999E-4 6075.8237 110-111 CGTGTCG 10 8.833439E-4 3037.9119 110-111 CAGCGCG 30 4.688738E-6 1663.3436 108-109 CGACGCG 15 0.0029484846 1663.3436 108-109 CGTCGAC 15 0.0029484846 1663.3436 108-109 CCACGCG 15 0.0029484846 1663.3436 108-109 CCGCACG 15 0.0029484846 1663.3436 108-109 CCGCTCC 190 0.0 1444.4825 108-109 CCGCGCC 160 0.0 1403.446 108-109 CTCCGCT 45 8.74278E-6 1350.1831 110-111 CCGTGCG 20 0.0052410555 1247.5077 108-109 CGCGTCG 20 0.0052410555 1247.5077 108-109 CCGTTCG 20 0.0052410555 1247.5077 108-109 AATCGCG 20 0.0052410555 1247.5077 108-109 CGTCGCG 25 0.0055190995 1215.1648 110-111 CGCCGCC 125 0.0 1215.1648 110-111 CCGCGCG 105 0.0 1188.1024 108-109 TTGTTCG 110 0.0 1134.0978 108-109 CCGCTCG 70 4.1247404E-8 1069.2922 108-109 CGTTGCG 30 0.007946639 1012.6374 110-111 >>END_MODULE