FastQCFastQC Report
Fri 27 May 2016
SRR939922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766147
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.2965488346231206No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17080.22293371898604314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.20191947498326038No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA14030.183124126309964No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC12290.16041307999639756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.12908749887423693No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8790.11472994085991331No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA8160.10650697581534613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG905.1113166E-639.7138521
TACCTGG9300.039.070752
GTACCTG10450.036.483541
ACCTGGG10750.033.8007433
GTATCAA61900.033.2018051
AATGCCG1901.0768417E-931.3121158
GTACATG41950.030.3889051
AGTACTT31750.029.32490312-13
TACATGG43700.029.0337432
ATCAACG70700.028.7302783
TATCAAC71900.028.6650072
CAACGCA71050.028.5887475
TCAACGC71050.028.5887474
TAGGACC11900.028.5320364
ACATGGG40850.028.434623
AACGCAG71850.028.2353786
GAGTACT30800.028.20123512-13
AGAGTAC54550.027.42895110-11
CATGGGG20050.027.3324344
GTACTTT32750.026.97710214-15
Produced by FastQC (version 0.11.5)