##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939922_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 766147 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83583307119913 34.0 31.0 34.0 31.0 34.0 2 32.920289448369566 34.0 33.0 34.0 31.0 34.0 3 32.96469737530787 34.0 33.0 34.0 31.0 34.0 4 36.366625464825944 37.0 37.0 37.0 35.0 37.0 5 36.26967801218304 37.0 37.0 37.0 35.0 37.0 6 36.40766197609597 37.0 37.0 37.0 35.0 37.0 7 36.406314976107716 37.0 37.0 37.0 35.0 37.0 8 36.436112129917625 37.0 37.0 37.0 35.0 37.0 9 38.2718460034432 39.0 39.0 39.0 37.0 39.0 10-11 38.26372549915355 39.0 39.0 39.0 37.0 39.0 12-13 38.25348790767307 39.0 39.0 39.0 37.0 39.0 14-15 39.78285302951 41.0 40.0 41.0 37.5 41.0 16-17 39.80750887231824 41.0 40.0 41.0 38.0 41.0 18-19 39.859800403838946 41.0 40.0 41.0 38.0 41.0 20-21 39.878570953093856 41.0 40.0 41.0 38.0 41.0 22-23 39.82795860324455 41.0 40.0 41.0 38.0 41.0 24-25 39.797948043913244 41.0 40.0 41.0 37.0 41.0 26-27 39.706013989482436 41.0 40.0 41.0 37.0 41.0 28-29 39.628611088994674 41.0 40.0 41.0 37.0 41.0 30-31 39.49195976751198 41.0 39.5 41.0 36.5 41.0 32-33 39.337248595896085 41.0 39.0 41.0 35.5 41.0 34-35 39.245081557455684 41.0 39.0 41.0 35.0 41.0 36-37 39.22856971312294 41.0 39.0 41.0 35.0 41.0 38-39 39.27864300193044 41.0 40.0 41.0 35.0 41.0 40-41 39.18485551728324 41.0 40.0 41.0 35.0 41.0 42-43 39.08341610683067 41.0 40.0 41.0 35.0 41.0 44-45 38.9192002318093 41.0 39.0 41.0 35.0 41.0 46-47 38.668805725272044 41.0 39.0 41.0 35.0 41.0 48-49 38.41198229582574 41.0 39.0 41.0 35.0 41.0 50-51 38.25187398762901 41.0 39.0 41.0 35.0 41.0 52-53 38.15330217308167 41.0 39.0 41.0 35.0 41.0 54-55 38.05613870445228 41.0 39.0 41.0 34.0 41.0 56-57 37.91656300944858 41.0 38.5 41.0 34.0 41.0 58-59 37.76675559651085 40.5 38.0 41.0 34.0 41.0 60-61 37.59780825350748 40.0 38.0 41.0 34.0 41.0 62-63 37.408817759516126 40.0 37.0 41.0 33.5 41.0 64-65 37.180278719358036 40.0 37.0 41.0 33.0 41.0 66-67 36.915522086492544 39.0 36.0 41.0 33.0 41.0 68-69 36.59180092071104 39.0 36.0 41.0 32.5 41.0 70-71 36.230269778515094 39.0 35.0 41.0 32.0 41.0 72-73 35.88708955331027 37.5 35.0 40.0 32.0 41.0 74-75 35.534137704644145 37.0 35.0 39.0 31.0 41.0 76-77 35.139349237156836 36.5 35.0 39.0 31.0 41.0 78-79 34.753658893136695 36.0 35.0 39.0 31.0 40.5 80-81 34.45017796845775 36.0 35.0 37.5 31.0 39.0 82-83 34.18555055361439 35.0 35.0 37.0 31.0 39.0 84-85 33.88154166237028 35.0 35.0 37.0 31.0 39.0 86-87 33.644117251650144 35.0 35.0 36.0 31.0 37.0 88-89 33.40787603423364 35.0 35.0 36.0 31.0 37.0 90-91 33.21080419292903 35.0 35.0 36.0 31.0 37.0 92-93 33.06208077562139 35.0 35.0 35.5 30.0 36.0 94-95 32.950127717004705 35.0 34.0 35.0 30.0 36.0 96-97 32.83029757996834 35.0 34.0 35.0 30.0 36.0 98-99 32.72552199512626 35.0 34.0 35.0 30.0 36.0 100-101 32.701586640683836 35.0 34.0 35.0 30.0 36.0 102-103 31.87782370746084 34.5 33.0 35.0 28.0 35.0 104-105 32.53044259130428 35.0 34.0 35.0 29.0 35.0 106-107 32.58861550068068 35.0 34.0 35.0 30.0 35.0 108-109 32.59330520122118 35.0 34.0 35.0 30.0 35.0 110-111 32.57853062140816 35.0 34.0 35.0 30.0 35.0 112-113 32.5066899694184 35.0 34.0 35.0 29.0 35.0 114-115 32.451951126872515 35.0 34.0 35.0 29.0 35.0 116-117 32.39180013757151 35.0 34.0 35.0 29.0 35.0 118-119 32.31583038242009 35.0 34.0 35.0 29.0 35.0 120-121 32.22733561574998 35.0 34.0 35.0 29.0 35.0 122-123 32.06554551541675 35.0 34.0 35.0 29.0 35.0 124-125 30.072054057511156 33.5 30.5 34.5 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07997900785403544 1101 2 0.14814812281863965 1101 3 0.10369717503669307 1101 4 0.07950229209879467 1101 5 0.0069611501498982875 1101 6 0.04390579971588693 1101 7 0.03799348263842717 1101 8 0.08295666540288948 1101 9 0.017857929667137284 1101 10-11 0.06310239124170636 1101 12-13 0.02790063100321305 1101 14-15 0.09451632359025552 1101 16-17 0.12407169047695277 1101 18-19 0.18384627050508584 1101 20-21 0.16175915158096643 1101 22-23 0.010999822222956368 1101 24-25 0.20051646324937877 1101 26-27 0.22668289984711265 1101 28-29 0.21263878549432746 1101 30-31 0.18823314772366473 1101 32-33 0.12766865854513298 1101 34-35 0.13136940954647258 1101 36-37 0.07778919100800152 1101 38-39 0.07419623129008102 1101 40-41 0.1689564035217117 1101 42-43 0.1481547921796107 1101 44-45 0.016019829408449482 1101 46-47 -0.020110685891189917 1101 48-49 -0.08158426667535679 1101 50-51 -0.03306765257428168 1101 52-53 -0.04146682613641417 1101 54-55 -0.10203459517783386 1101 56-57 -0.1355089415148072 1101 58-59 -0.17645044108127905 1101 60-61 -0.16658927954524216 1101 62-63 -0.07918882458154997 1101 64-65 -0.1841050309775838 1101 66-67 -0.2448091123050844 1101 68-69 -0.3466718564460649 1101 70-71 -0.21715255956281965 1101 72-73 -0.24318445099827102 1101 74-75 -0.18442254031713645 1101 76-77 -0.2757783716133986 1101 78-79 -0.20310103845254446 1101 80-81 -0.1743122868696645 1101 82-83 -0.08246681293383062 1101 84-85 -0.02714808361515253 1101 86-87 -0.07176712974600008 1101 88-89 -0.10691385912527096 1101 90-91 -0.10574205196975583 1101 92-93 -0.08548394473504572 1101 94-95 -0.04593150582569905 1101 96-97 0.038245754657268094 1101 98-99 -0.03720387841061523 1101 100-101 -0.052735325598668226 1101 102-103 0.010030947479613417 1101 104-105 0.0037110723132514067 1101 106-107 -0.10536524522545676 1101 108-109 -0.07929389270051956 1101 110-111 -0.09038354685315397 1101 112-113 -0.19412535046539148 1101 114-115 -0.1255873740289104 1101 116-117 -0.1568428120539309 1101 118-119 -0.07795364180103093 1101 120-121 -0.03583038238609504 1101 122-123 -0.0974857114090284 1101 124-125 0.1876039908902314 1102 1 -0.03389671621125956 1102 2 0.03988939015232518 1102 3 -0.011925082291725175 1102 4 -0.06145347409394475 1102 5 -0.1307955639101337 1102 6 -0.06197448815492379 1102 7 -0.03658085473211514 1102 8 -0.04679144763905896 1102 9 -0.019565275528577786 1102 10-11 0.12482347251255277 1102 12-13 -0.015310708298180487 1102 14-15 0.061961908986077674 1102 16-17 0.11178452179168374 1102 18-19 0.03052215254826507 1102 20-21 -0.05492506818382026 1102 22-23 0.011037122556466272 1102 24-25 0.036572720928859326 1102 26-27 0.023545283557837138 1102 28-29 0.01664528014062938 1102 30-31 0.02356972838015281 1102 32-33 0.04209291692673389 1102 34-35 -0.022062221136344817 1102 36-37 0.12792083924392017 1102 38-39 0.09092213966291496 1102 40-41 -0.04970034564450998 1102 42-43 0.015133026337942113 1102 44-45 0.022196325810064366 1102 46-47 0.09058573327679653 1102 48-49 0.2230892456995761 1102 50-51 0.18019151891948582 1102 52-53 0.17935334237332512 1102 54-55 0.09023984751129888 1102 56-57 0.061044068841525245 1102 58-59 0.06139623260663285 1102 60-61 0.12905535798328316 1102 62-63 0.12020559353324245 1102 64-65 0.005617630072094926 1102 66-67 0.060562135720196864 1102 68-69 0.06533226853198926 1102 70-71 0.05087886048281831 1102 72-73 -0.0016384618811997598 1102 74-75 -0.045432721114046615 1102 76-77 -0.07849910182228825 1102 78-79 -0.032399353355117455 1102 80-81 -0.18238671200569456 1102 82-83 -0.1400870516559678 1102 84-85 -0.05966300374856104 1102 86-87 -0.078178398835 1102 88-89 -0.0667392057989531 1102 90-91 -0.035430923305547424 1102 92-93 -0.08443075884773776 1102 94-95 -0.01545493921168628 1102 96-97 -0.0632374821340278 1102 98-99 -0.10256283926720755 1102 100-101 -0.12273927504574544 1102 102-103 -0.07979922629605696 1102 104-105 0.0451122483708275 1102 106-107 0.049191977874421866 1102 108-109 -0.010301440532714423 1102 110-111 -0.09464236728495479 1102 112-113 -0.07653495201007132 1102 114-115 -0.006915265901824341 1102 116-117 0.044877391562003766 1102 118-119 -0.12949612029848367 1102 120-121 -0.02251855159796179 1102 122-123 -0.010014235193473553 1102 124-125 0.10831884081123988 1103 1 -0.004733896882235911 1103 2 0.04644012371136341 1103 3 0.05559312843978148 1103 4 0.07065640586912281 1103 5 -0.0452923321465164 1103 6 0.041400909403300545 1103 7 0.02236548773223035 1103 8 0.047219530097677875 1103 9 0.11729734886867504 1103 10-11 0.1038760014958271 1103 12-13 0.09020124409743602 1103 14-15 0.014869213797808811 1103 16-17 0.050606723494773576 1103 18-19 0.012820552055814005 1103 20-21 -0.011039770143398187 1103 22-23 -0.02960486985145394 1103 24-25 0.04348382178040566 1103 26-27 0.038136952789393774 1103 28-29 0.10256145947786166 1103 30-31 -0.06463561685427521 1103 32-33 0.0456812005011642 1103 34-35 0.06460034170350326 1103 36-37 -0.01569258026977849 1103 38-39 -0.043946281389715125 1103 40-41 0.06305870842340511 1103 42-43 -0.021598788579474615 1103 44-45 0.06530061430908773 1103 46-47 0.2307096175488752 1103 48-49 0.14801229894673185 1103 50-51 0.052581741552089056 1103 52-53 0.036320870843624675 1103 54-55 0.14857959307403945 1103 56-57 0.10880363588778863 1103 58-59 -0.024798237486727714 1103 60-61 0.11146404783657715 1103 62-63 0.10957155228978621 1103 64-65 -0.058981733834748695 1103 66-67 -0.04728643307020519 1103 68-69 -0.016699382232346238 1103 70-71 0.03079832288150186 1103 72-73 0.019099970456956328 1103 74-75 0.10058400198023776 1103 76-77 0.18646447671470412 1103 78-79 0.062218221283224295 1103 80-81 0.07548750570231277 1103 82-83 0.032549400585743626 1103 84-85 -0.030001312971911887 1103 86-87 -0.038558515794058223 1103 88-89 0.009979271681302748 1103 90-91 0.038993258493981386 1103 92-93 -0.021519093720002047 1103 94-95 -0.005982793936219366 1103 96-97 0.10368054392527881 1103 98-99 0.05921514370191261 1103 100-101 0.021155000115847145 1103 102-103 -0.05885047283345912 1103 104-105 -0.06578212938643446 1103 106-107 0.030454161110291977 1103 108-109 0.003359172989391368 1103 110-111 -0.03820577140930226 1103 112-113 0.01516121091185596 1103 114-115 -0.0777704008151332 1103 116-117 -0.02827332147790429 1103 118-119 0.036279246481036864 1103 120-121 0.10071808531596105 1103 122-123 0.08962924485166468 1103 124-125 0.13792410396913724 1104 1 0.07289119871619221 1104 2 0.11072686447816693 1104 3 0.1513559088962424 1104 4 0.03271766484463967 1104 5 -0.007420489372265138 1104 6 0.012059832391024372 1104 7 0.012875847050167977 1104 8 0.024743369825991124 1104 9 -0.027482853213598446 1104 10-11 0.0563761790652606 1104 12-13 0.05467703436398352 1104 14-15 0.006788152940224279 1104 16-17 0.05552002831788627 1104 18-19 0.08121038550393678 1104 20-21 0.10713681480829962 1104 22-23 0.08763803026202766 1104 24-25 0.17981048015499113 1104 26-27 0.15294467400373435 1104 28-29 0.15060307514493587 1104 30-31 0.18606144639512934 1104 32-33 0.21730824853742092 1104 34-35 0.17501920734043352 1104 36-37 0.12784133218766414 1104 38-39 0.08290931915008315 1104 40-41 0.13022992004156464 1104 42-43 0.280290715332832 1104 44-45 0.24742408039828234 1104 46-47 0.19975637529627477 1104 48-49 0.13372828366419753 1104 50-51 0.2564727420030337 1104 52-53 0.19996803130197094 1104 54-55 0.1264395758621859 1104 56-57 0.2371016783293669 1104 58-59 0.14927720391782628 1104 60-61 0.07450772000542116 1104 62-63 0.23137633493654874 1104 64-65 0.22042913209289594 1104 66-67 0.22964382922049253 1104 68-69 0.239473426684782 1104 70-71 0.2678155198613368 1104 72-73 0.17351641123879347 1104 74-75 0.08625249719908368 1104 76-77 0.09720486690493857 1104 78-79 0.1762743695627904 1104 80-81 0.11980635762922986 1104 82-83 0.23116535011968864 1104 84-85 0.12942382458245305 1104 86-87 0.14260772718368742 1104 88-89 0.17111774775567312 1104 90-91 0.13670765086791192 1104 92-93 0.16842155679527337 1104 94-95 0.13103184274020663 1104 96-97 0.1704668841284871 1104 98-99 0.26932479075995985 1104 100-101 0.1732729491641507 1104 102-103 0.18425116130083907 1104 104-105 0.2367499280898926 1104 106-107 0.21492360843163283 1104 108-109 0.2467614362976036 1104 110-111 0.16567118866813502 1104 112-113 0.2789104531446398 1104 114-115 0.250546802606749 1104 116-117 0.25598518987910523 1104 118-119 0.2945704999890637 1104 120-121 0.16015305113177902 1104 122-123 0.30430531691677487 1104 124-125 0.2992475826120753 1105 1 0.014056500163007968 1105 2 0.044177889177660745 1105 3 -0.03258785032291911 1105 4 -0.014523649532534932 1105 5 -0.18693591478732685 1105 6 0.007421223346049999 1105 7 -0.0753722777340684 1105 8 -0.005319712746270966 1105 9 -0.05514032426152227 1105 10-11 0.075067137229901 1105 12-13 0.044679545113133656 1105 14-15 0.0400077179529319 1105 16-17 0.10773968748369356 1105 18-19 -0.0014222918961763753 1105 20-21 0.10214120861929388 1105 22-23 0.11208488084106705 1105 24-25 -0.054484783944438675 1105 26-27 0.07973436737849937 1105 28-29 0.0025614594778602395 1105 30-31 0.022668168925960686 1105 32-33 0.001936971507632279 1105 34-35 0.0990878763295413 1105 36-37 0.016371131641577108 1105 38-39 0.026667753697999785 1105 40-41 0.051493611470498024 1105 42-43 0.061088192029949084 1105 44-45 0.09161640378277269 1105 46-47 -5.692374834396219E-4 1105 48-49 -0.0638990583939858 1105 50-51 -0.08606091772768565 1105 52-53 -0.06545659914714008 1105 54-55 -0.10349796925283528 1105 56-57 0.025493386580308197 1105 58-59 0.03247323065730967 1105 60-61 0.16148713186242958 1105 62-63 0.2032465292057637 1105 64-65 0.19182620707014308 1105 66-67 0.09477266203598589 1105 68-69 0.14181862330782025 1105 70-71 0.03645390921575853 1105 72-73 -0.027345089561507052 1105 74-75 0.038534140484394186 1105 76-77 0.05317731143307469 1105 78-79 0.11884333670335678 1105 80-81 0.029065529709697557 1105 82-83 0.04746168128750128 1105 84-85 0.013119647323570405 1105 86-87 -0.04294448070633905 1105 88-89 0.0075785329924684675 1105 90-91 -0.10689778490398538 1105 92-93 -0.05884596352609606 1105 94-95 0.040831610495907 1105 96-97 0.021362907729525205 1105 98-99 -0.12834256636272556 1105 100-101 0.0805501986384698 1105 102-103 0.04322708949341347 1105 104-105 0.07452257975484144 1105 106-107 0.07606819619800831 1105 108-109 0.004074777790876283 1105 110-111 -0.07497862459489824 1105 112-113 -0.039132417897008054 1105 114-115 0.04245120583306772 1105 116-117 0.0593998087160017 1105 118-119 0.006639357284356606 1105 120-121 -0.16303999409309 1105 122-123 -0.07117407924805974 1105 124-125 -0.029619755679988202 1106 1 -0.08489893265146264 1106 2 -0.024899726805053035 1106 3 -0.027559016312480367 1106 4 -0.06623086640385623 1106 5 -0.13056428319316637 1106 6 -0.04151899363178302 1106 7 -0.054146946409225905 1106 8 -0.06401784576311798 1106 9 -0.04985662498731358 1106 10-11 -0.05285392300960012 1106 12-13 0.008300001283913616 1106 14-15 -0.17122727542817273 1106 16-17 -0.06345559256378408 1106 18-19 -0.13122136677331042 1106 20-21 -0.05636254208443603 1106 22-23 -0.07717197769729722 1106 24-25 -0.019546179845285394 1106 26-27 -0.008515202774120212 1106 28-29 -0.05362149574192898 1106 30-31 -0.04030028864467994 1106 32-33 -0.06901257277806394 1106 34-35 -0.012679290938145016 1106 36-37 0.02892305568411757 1106 38-39 -0.02863914972709125 1106 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-0.16383428509841025 2216 20-21 -0.18016447364901467 2216 22-23 -0.154774091060645 2216 24-25 -0.10556769880624728 2216 26-27 -0.14404056521883746 2216 28-29 -0.2781262393759718 2216 30-31 -0.29003355990448654 2216 32-33 -0.3197350618167576 2216 34-35 -0.2944152733874361 2216 36-37 -0.23400357606724498 2216 38-39 -0.30366695963304124 2216 40-41 -0.270970003867923 2216 42-43 -0.3059892795075996 2216 44-45 -0.2951598960345052 2216 46-47 -0.2927668199319058 2216 48-49 -0.2507737469361686 2216 50-51 -0.2191789458643001 2216 52-53 -0.24912950135897205 2216 54-55 -0.3719330774165428 2216 56-57 -0.3462105911352822 2216 58-59 -0.4691070583755419 2216 60-61 -0.3915945435569981 2216 62-63 -0.575444645197841 2216 64-65 -0.5031479818617655 2216 66-67 -0.5770683571178736 2216 68-69 -0.5120412174813325 2216 70-71 -0.5193896782671459 2216 72-73 -0.4725783475060865 2216 74-75 -0.3777025546766737 2216 76-77 -0.5022724017062927 2216 78-79 -0.46625364646833845 2216 80-81 -0.5181747943342074 2216 82-83 -0.3992940463418364 2216 84-85 -0.34196014976566147 2216 86-87 -0.2635045297729235 2216 88-89 -0.35716635909938077 2216 90-91 -0.4170267124668854 2216 92-93 -0.37101326269171153 2216 94-95 -0.31171819080997665 2216 96-97 -0.3276800383100067 2216 98-99 -0.3838865154561475 2216 100-101 -0.3892147545310607 2216 102-103 -0.261047708937479 2216 104-105 -0.22082440022241911 2216 106-107 -0.23312539959044187 2216 108-109 -0.184792282430692 2216 110-111 -0.16798440014083127 2216 112-113 -0.18252312335694398 2216 114-115 -0.2849391929607137 2216 116-117 -0.39348413092187684 2216 118-119 -0.26091791900907424 2216 120-121 -0.3081882799570579 2216 122-123 -0.3952365113662246 2216 124-125 -0.4402821545759892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69.0 3 6.0 4 8.0 5 27.0 6 45.0 7 126.0 8 269.0 9 485.0 10 1086.0 11 1768.0 12 2161.0 13 3703.0 14 6519.0 15 7124.0 16 2523.0 17 1585.0 18 1353.0 19 1101.0 20 1096.0 21 1085.0 22 1189.0 23 1250.0 24 1543.0 25 1926.0 26 2639.0 27 3318.0 28 4175.0 29 5471.0 30 6951.0 31 9119.0 32 11626.0 33 15373.0 34 22622.0 35 36492.0 36 80428.0 37 266549.0 38 259969.0 39 3368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.709203322167184 20.30884875575387 15.653010550831196 22.328937371247743 2 13.28622313994573 20.67436144760731 44.62720600615809 21.41220940628887 3 19.072971635991525 26.923945404733036 29.198182594201892 24.804900365073543 4 11.359308331168823 17.779094612391617 39.90383046595497 30.957766590484592 5 11.915557129768438 38.982550198729854 34.70355204291153 14.398340628590175 6 28.633147424710927 38.01555054056206 19.900619593889946 13.450682440837072 7 27.105111682222866 32.70129622644218 22.322609107651665 17.870982983683287 8 24.315046590275756 37.878762169661954 20.601659994752968 17.204531245309322 9 25.857309367523463 17.657707985543244 22.3245669564718 34.16041569046149 10-11 23.702827264219533 27.090036246307825 29.645942619366778 19.561193870105868 12-13 26.40126503138432 24.778338882747043 29.408651342366415 19.411744743502226 14-15 20.823223219564913 28.823776638164738 27.498965603206692 22.854034539063655 16-17 19.305524012394503 30.26838226656538 30.49418779188301 19.931905929157107 18-19 20.13764982438096 27.706758624650362 32.82026817307906 19.335323377889623 20-21 21.36069144072559 27.45250195631048 31.20287478111839 19.983931821845545 22-23 20.01758155124885 29.22133437056219 31.47476192465124 19.286322153537718 24-25 20.511533687399417 27.684047578336795 32.87678474235362 18.92763399191017 26-27 19.043995473453528 28.964023875313742 32.35638852596173 19.635592125271 28-29 19.056721490784405 29.122935937881373 30.952284613788215 20.868057957546007 30-31 20.00236247090963 27.74957025218398 32.03288663924808 20.21518063765831 32-33 19.088308118415917 28.75114044693773 32.72570407506654 19.43484735957982 34-35 19.48999343468029 28.975901491489232 32.50746919324882 19.02663588058166 36-37 19.038644020011823 28.314866468184302 32.29425945673611 20.35223005506776 38-39 20.111871481582515 29.093763990461362 31.20941542549928 19.58494910245684 40-41 21.6590941425079 27.210900780137493 32.12294768497429 19.007057392380315 42-43 19.32324997683212 29.20255512323353 32.05664187159905 19.417553028335295 44-45 19.422643435267645 29.288569948064797 31.072104961580482 20.21668165508708 46-47 20.910282230433587 28.022298592828793 30.62571543058969 20.441703746147933 48-49 20.525042844258348 28.714789720510552 30.143301481308416 20.61686595392268 50-51 19.618754625417836 29.97766747112499 30.150610783570254 20.252967119886915 52-53 19.664046194790295 29.502628085732894 30.54100583830518 20.29231988117163 54-55 19.827591832898907 28.59503463434563 30.87018548659722 20.707188046158244 56-57 20.455147641379526 28.924149021010326 29.14930163532586 21.471401702284286 58-59 20.775190661844267 29.34639174988612 28.873767044705517 21.004650543564093 60-61 20.124466975658716 28.024843796294967 31.286163099248576 20.564526128797738 62-63 20.370568572349693 28.074116324935034 30.93916702669331 20.616148076021965 64-65 20.609230343524153 28.98197082283165 29.524686515773084 20.884112317871114 66-67 20.591674965770277 29.921216163477766 28.897783323565847 20.589325547186114 68-69 21.304136151417417 28.040702371738064 30.674009034819687 19.981152442024833 70-71 21.814221030689936 28.027911092779846 29.961613110799885 20.196254765730338 72-73 20.935995311604692 29.06720250813486 29.741811949926056 20.25499023033439 74-75 20.11872395245299 29.65318666000128 30.53754697205628 19.690542415489457 76-77 20.567267117145928 28.180362254241025 30.90425205606757 20.348118572545477 78-79 20.292450404426305 28.21194888187254 30.359578514306 21.136022199395153 80-81 20.609817698170193 29.0106859388603 30.13044494072286 20.249051422246644 82-83 20.270130927876764 28.88486152135295 31.06871135695891 19.776296193811373 84-85 20.177328893802365 28.64456820949505 30.98452385769311 20.193579039009485 86-87 19.31156814553865 30.034314563654235 29.42653302825698 21.22758426255014 88-89 20.323775985548465 28.58576748326366 30.122809330324333 20.967647200863542 90-91 20.357505294380616 28.819638644246993 30.727769311844728 20.095086749527667 92-93 19.6921739561729 29.817254391128596 30.695284325331823 19.79528732736668 94-95 19.614940079528495 29.74532739099421 30.282957238261126 20.356775291216167 96-97 20.64636420947938 29.090631432349145 29.93407270406332 20.328931654108153 98-99 20.761441461666877 28.62587598333984 30.607174416130757 20.00550813886253 100-101 20.015414796377197 28.76673797587147 30.751213539960347 20.466633687790985 102-103 19.754263547678903 29.46494532373725 30.18680512591595 20.5939860026679 104-105 20.36068126507978 28.68925585423428 30.033864855146003 20.916198025539938 106-107 20.263193275994627 28.31197599240821 29.952903767307014 21.47192696429014 108-109 19.728080726075234 29.24440691173294 29.648180863581576 21.379331498610252 110-111 19.42970367851635 29.48484585448183 30.20053895619496 20.884911510806862 112-113 19.938687545361034 28.393723338154437 29.827849978331134 21.839739138153394 114-115 19.69057665260197 28.93294304613286 28.843398892445855 22.533081408819317 116-117 20.339991855231972 28.756578466486367 28.22468856704293 22.678741111238736 118-119 20.22955139780708 28.024796824106097 29.645439873444566 22.100211904642258 120-121 19.625591844859148 28.234825766747047 30.13075243835187 22.00882995004193 122-123 20.318963008018127 28.71776531412707 29.227398683528705 21.735872994326094 124-125 20.659398176307665 28.792593501431856 28.66454868444066 21.88345963781982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45.0 1 29.5 2 57.0 3 87.5 4 48.0 5 29.0 6 76.5 7 191.5 8 347.5 9 385.5 10 311.5 11 273.5 12 299.5 13 374.5 14 479.5 15 580.0 16 678.5 17 824.0 18 989.0 19 1172.0 20 1405.5 21 1740.0 22 2148.5 23 2615.5 24 3302.0 25 4194.0 26 5274.0 27 6625.5 28 8149.5 29 9959.0 30 12413.0 31 15531.0 32 19549.0 33 24711.0 34 31237.0 35 37901.5 36 42381.0 37 43414.0 38 40718.5 39 35997.5 40 31762.5 41 28823.0 42 26747.0 43 25255.0 44 24345.0 45 23802.5 46 23283.5 47 23765.0 48 25532.0 49 24514.0 50 21625.0 51 19474.0 52 17222.0 53 15562.5 54 14131.0 55 12312.0 56 10419.5 57 8858.0 58 7401.0 59 5994.5 60 4628.5 61 3562.5 62 2793.0 63 2029.5 64 1446.5 65 1139.5 66 855.0 67 628.0 68 451.5 69 304.0 70 235.0 71 170.5 72 137.5 73 122.5 74 93.0 75 76.0 76 49.0 77 25.5 78 15.0 79 8.5 80 5.0 81 3.5 82 2.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 1.0 97 2.5 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01840377890926937 2 0.0 3 0.0 4 0.0 5 0.13548313835334475 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.305232546756693E-4 18-19 0.0 20-21 0.004764098795661929 22-23 3.9156976402700787E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 5.873546460405118E-4 92-93 0.0 94-95 8.484011553918505E-4 96-97 0.0 98-99 0.0010441860374053543 100-101 0.0 102-103 1.305232546756693E-4 104-105 0.0017620639381215357 106-107 0.00730930226183748 108-109 6.526162733783465E-5 110-111 0.004764098795661929 112-113 0.009267151081972519 114-115 0.005808284833067284 116-117 0.0014357558014323622 118-119 6.526162733783465E-5 120-121 5.873546460405118E-4 122-123 0.0010441860374053543 124-125 0.0011747092920810236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 766147.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.03308593581404 #Duplication Level Percentage of deduplicated Percentage of total 1 88.7928853980115 69.2878285675192 2 8.072974528732683 12.599182303164703 3 1.6047257034274447 3.7566509615689023 4 0.5651360511681325 1.7639724018491794 5 0.2664124971235828 1.0394494641209673 6 0.15263835313275437 0.714650503626561 7 0.10247616778326027 0.5597572124902831 8 0.06899575523047984 0.43071613576851386 9 0.05488148435175607 0.3854314426235053 >10 0.279741675501716 4.063726465744924 >50 0.021239203211356406 1.1272526726003043 >100 0.015552018301874767 2.3998712041035755 >500 0.001505034015092033 0.806829622129319 >1k 8.361300083844628E-4 1.0646810426901365 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2272 0.2965488346231206 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1708 0.22293371898604314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1547 0.20191947498326038 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1403 0.183124126309964 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1229 0.16041307999639756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 989 0.12908749887423693 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 879 0.11472994085991331 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 816 0.10650697581534613 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.305232546756693E-4 0.0 10-11 0.0 0.0 0.0 1.305232546756693E-4 0.0 12-13 0.0 0.0 0.0 1.305232546756693E-4 0.0 14-15 0.0 0.0 0.0 1.305232546756693E-4 0.0 16-17 0.0 0.0 0.0 1.305232546756693E-4 0.0 18-19 0.0 0.0 0.0 1.305232546756693E-4 0.0 20-21 0.0 0.0 0.0 1.305232546756693E-4 0.0 22-23 0.0 0.0 0.0 1.305232546756693E-4 0.0 24-25 0.0 0.0 0.0 3.263081366891732E-4 6.526162733783465E-5 26-27 0.0 0.0 0.0 3.9156976402700787E-4 1.305232546756693E-4 28-29 0.0 0.0 0.0 0.0011747092920810238 1.305232546756693E-4 30-31 0.0 0.0 0.0 0.0030020348575403935 1.305232546756693E-4 32-33 0.0 0.0 0.0 0.00646090110644563 1.305232546756693E-4 34-35 0.0 0.0 0.0 0.012726017330877755 1.305232546756693E-4 36-37 0.0 0.0 0.0 0.020231104474728742 2.610465093513386E-4 38-39 0.0 0.0 0.0 0.03530654038976854 2.610465093513386E-4 40-41 0.0 0.0 0.0 0.05534185998248378 2.610465093513386E-4 42-43 0.0 0.0 0.0 0.07381090051909098 2.610465093513386E-4 44-45 0.0 0.0 0.0 0.09599985381395476 2.610465093513386E-4 46-47 0.0 0.0 0.0 0.12327921404116965 2.610465093513386E-4 48-49 0.0 0.0 0.0 0.15147223705111423 2.610465093513386E-4 50-51 0.0 0.0 0.0 0.18260203329126135 2.610465093513386E-4 52-53 0.0 0.0 0.0 0.21653807950693538 2.610465093513386E-4 54-55 0.0 0.0 0.0 0.25863182913983873 2.610465093513386E-4 56-57 0.0 0.0 0.0 0.30842645079860653 2.610465093513386E-4 58-59 0.0 0.0 0.0 0.3599831363954959 2.610465093513386E-4 60-61 0.0 0.0 0.0 0.4200238335463038 2.610465093513386E-4 62-63 0.0 0.0 0.0 0.4852201992568006 2.610465093513386E-4 64-65 0.0 0.0 0.0 0.5504818265946352 2.610465093513386E-4 66-67 0.0 0.0 0.0 0.6246842968777533 2.610465093513386E-4 68-69 0.0 0.0 0.0 0.7167031914241001 2.610465093513386E-4 70-71 0.0 0.0 0.0 0.817010312642352 2.610465093513386E-4 72-73 0.0 0.0 0.0 0.9153595850404688 2.610465093513386E-4 74-75 0.0 0.0 0.0 1.0145572585939775 2.610465093513386E-4 76-77 0.0 0.0 0.0 1.1266114727330394 2.610465093513386E-4 78-79 0.0 0.0 0.0 1.2581789134461143 2.610465093513386E-4 80-81 0.0 0.0 0.0 1.4029944645087693 2.610465093513386E-4 82-83 0.0 0.0 0.0 1.553096207385789 2.610465093513386E-4 84-85 0.0 0.0 0.0 1.70104431656066 2.610465093513386E-4 86-87 0.0 0.0 0.0 1.856954344270747 2.610465093513386E-4 88-89 0.0 0.0 0.0 2.0221315230628063 2.610465093513386E-4 90-91 0.0 0.0 0.0 2.2158280330014994 2.610465093513386E-4 92-93 0.0 0.0 0.0 2.42166320562503 2.610465093513386E-4 94-95 0.0 0.0 0.0 2.620906953887439 2.610465093513386E-4 96-97 0.0 0.0 0.0 2.8249800625728483 2.610465093513386E-4 98-99 0.0 0.0 0.0 3.050915816416432 2.610465093513386E-4 100-101 0.0 0.0 0.0 3.3077203199908114 2.610465093513386E-4 102-103 0.0 0.0 0.0 3.582080201319068 2.610465093513386E-4 104-105 0.0 0.0 0.0 3.8533075245351087 2.610465093513386E-4 106-107 0.0 0.0 0.0 4.129103161664798 2.610465093513386E-4 108-109 0.0 0.0 0.0 4.426630920697987 2.610465093513386E-4 110-111 0.0 0.0 0.0 4.751176993449038 2.610465093513386E-4 112-113 0.0 0.0 0.0 5.10796231010498 4.5683139136484254E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 90 5.1113166E-6 39.713852 1 TACCTGG 930 0.0 39.07075 2 GTACCTG 1045 0.0 36.48354 1 ACCTGGG 1075 0.0 33.800743 3 GTATCAA 6190 0.0 33.201805 1 AATGCCG 190 1.0768417E-9 31.312115 8 GTACATG 4195 0.0 30.388905 1 AGTACTT 3175 0.0 29.324903 12-13 TACATGG 4370 0.0 29.033743 2 ATCAACG 7070 0.0 28.730278 3 TATCAAC 7190 0.0 28.665007 2 CAACGCA 7105 0.0 28.588747 5 TCAACGC 7105 0.0 28.588747 4 TAGGACC 1190 0.0 28.532036 4 ACATGGG 4085 0.0 28.43462 3 AACGCAG 7185 0.0 28.235378 6 GAGTACT 3080 0.0 28.201235 12-13 AGAGTAC 5455 0.0 27.428951 10-11 CATGGGG 2005 0.0 27.332434 4 GTACTTT 3275 0.0 26.977102 14-15 >>END_MODULE