FastQCFastQC Report
Fri 27 May 2016
SRR939921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401831
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34050.242896611645769No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17100.1219833203859809No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15320.10928564142182616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG108.8871905E-43031.9092110-111
CGTCGCG401.8189894E-122511.7908108-109
CCGCGCC2650.02274.8293108-109
CGTCGCT402.0354491E-92273.932110-111
CATCGCG600.02093.159108-109
CGTGTCG150.00199939922021.2726110-111
CCGCTCC4100.01899.1589108-109
CGCCGCC1450.01672.7775110-111
CCGCACG501.0568328E-81507.0745108-109
CCGTTCG804.3655746E-111255.8954108-109
TTGTTCG2050.01225.2638108-109
CCGCTCG1250.01205.6595108-109
CTCCGCC2450.01113.7626110-111
CGTCGCC1150.01092.0829108-109
CGCGTCT854.1443855E-81070.0856110-111
CGTCGGC602.0971871E-51010.6363110-111
CGTCGAT300.0079949731010.6363110-111
CGTCGTC956.46487E-8957.44495110-111
CGTCTCG703.3294382E-5866.2597110-111
CCGTTCC1850.0814.6348108-109
Produced by FastQC (version 0.11.5)