FastQCFastQC Report
Fri 27 May 2016
SRR939921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401831
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36290.2588757132635817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.1742720770192698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.12283934368693517No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16700.11912990938280008No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15540.11085501747357562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG43050.030.1727621
TACATGG43450.029.3401722
TAGGACC14050.027.5597764
ACATGGG41150.027.3609163
GGTCCGT900.0095335526.4422326
GTGTAGC10450.026.2284891
GTAGGAC34950.025.055833
GTCCTAC35850.024.2658441
CATGGGG21300.024.052354
TCCTACA41900.023.8854792
CTACAGT41800.023.5150724
GAGTACT39950.023.45548412-13
TGTAGGA41000.023.392722
CCTACAG42200.022.304033
AGAGTAC59400.022.23551410-11
GTATCAA89800.021.9625841
GTACTTT42750.021.85080314-15
TAGCCCT14500.021.7744164
AGTGGAC27050.021.334638
GACGTGG18700.021.3163997
Produced by FastQC (version 0.11.5)