##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939921_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1401831 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84404967503215 34.0 31.0 34.0 31.0 34.0 2 32.93077125559358 34.0 31.0 34.0 31.0 34.0 3 32.961325580615636 34.0 33.0 34.0 31.0 34.0 4 36.36738380018704 37.0 37.0 37.0 35.0 37.0 5 36.26881485714041 37.0 37.0 37.0 35.0 37.0 6 36.397986633196155 37.0 37.0 37.0 35.0 37.0 7 36.39438634186289 37.0 37.0 37.0 35.0 37.0 8 36.4215287006779 37.0 37.0 37.0 35.0 37.0 9 38.27080011784587 39.0 39.0 39.0 37.0 39.0 10-11 38.255488357726435 39.0 39.0 39.0 37.0 39.0 12-13 38.23715733208925 39.0 39.0 39.0 37.0 39.0 14-15 39.80584606846332 41.0 40.0 41.0 38.0 41.0 16-17 39.82142426583518 41.0 40.0 41.0 38.0 41.0 18-19 39.843731876381675 41.0 40.0 41.0 38.0 41.0 20-21 39.84783650810975 41.0 40.0 41.0 38.0 41.0 22-23 39.80089647040192 41.0 40.0 41.0 37.5 41.0 24-25 39.76913479584914 41.0 40.0 41.0 37.0 41.0 26-27 39.68089912407416 41.0 40.0 41.0 37.0 41.0 28-29 39.60947075646065 41.0 40.0 41.0 37.0 41.0 30-31 39.514528498799066 41.0 40.0 41.0 37.0 41.0 32-33 39.395525922882285 41.0 39.0 41.0 36.0 41.0 34-35 39.307884830625085 41.0 39.0 41.0 36.0 41.0 36-37 39.307771050861334 41.0 39.5 41.0 35.5 41.0 38-39 39.377600081607554 41.0 40.0 41.0 36.0 41.0 40-41 39.328011008459654 41.0 40.0 41.0 36.0 41.0 42-43 39.24699517987547 41.0 40.0 41.0 35.5 41.0 44-45 39.13051608931462 41.0 40.0 41.0 35.0 41.0 46-47 38.97982281744376 41.0 39.5 41.0 35.0 41.0 48-49 38.812956055330496 41.0 39.0 41.0 35.0 41.0 50-51 38.68302955206441 41.0 39.0 41.0 35.0 41.0 52-53 38.58657213316013 41.0 39.0 41.0 35.0 41.0 54-55 38.48127556032075 41.0 39.0 41.0 35.0 41.0 56-57 38.35301009893489 41.0 39.0 41.0 35.0 41.0 58-59 38.20932230775322 41.0 38.0 41.0 34.5 41.0 60-61 38.03075299376316 40.0 38.0 41.0 34.0 41.0 62-63 37.83261249037866 40.0 37.0 41.0 34.0 41.0 64-65 37.600793890276364 40.0 37.0 41.0 34.0 41.0 66-67 37.33446221406147 39.0 36.5 41.0 34.0 41.0 68-69 37.008014518155186 39.0 36.0 41.0 33.0 41.0 70-71 36.644861256456736 39.0 35.0 41.0 33.0 41.0 72-73 36.28325418684564 37.5 35.0 40.0 32.5 41.0 74-75 35.90849289250987 37.0 35.0 39.0 32.0 41.0 76-77 35.50850601820048 36.5 35.0 39.0 32.0 41.0 78-79 35.11117138941855 36.0 35.0 39.0 32.0 40.0 80-81 34.79606956901367 36.0 35.0 37.0 32.0 39.0 82-83 34.52798019162081 35.0 35.0 37.0 32.0 39.0 84-85 34.23513747377537 35.0 35.0 36.5 32.0 38.5 86-87 33.99910973576701 35.0 35.0 36.0 32.0 37.0 88-89 33.77676160678427 35.0 35.0 36.0 31.0 37.0 90-91 33.593589740846085 35.0 35.0 36.0 31.0 37.0 92-93 33.4465777258457 35.0 35.0 35.0 31.0 36.0 94-95 33.33860536683808 35.0 35.0 35.0 31.0 36.0 96-97 33.24093738831571 35.0 34.0 35.0 31.0 36.0 98-99 33.15176972117181 35.0 34.0 35.0 31.0 36.0 100-101 33.112719721564154 35.0 34.0 35.0 31.0 36.0 102-103 32.28168195738288 34.5 33.0 35.0 28.5 35.0 104-105 32.94678887825993 35.0 34.0 35.0 30.5 35.0 106-107 33.00952967939787 35.0 34.0 35.0 31.0 35.0 108-109 33.016560840786084 35.0 34.0 35.0 31.0 35.0 110-111 32.99341361405191 35.0 34.0 35.0 31.0 35.0 112-113 32.92669194788816 35.0 34.0 35.0 31.0 35.0 114-115 32.87454086833577 35.0 34.0 35.0 31.0 35.0 116-117 32.82152805866042 35.0 34.0 35.0 31.0 35.0 118-119 32.74614379336739 35.0 34.0 35.0 30.5 35.0 120-121 32.65836930414579 35.0 34.0 35.0 30.0 35.0 122-123 32.503685536986985 35.0 34.0 35.0 30.0 35.0 124-125 30.516155656423635 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07113808264308119 1101 2 0.12770109887694758 1101 3 0.15638861420674743 1101 4 0.08676963473493515 1101 5 -0.01627711889283745 1101 6 -0.02808519887904737 1101 7 0.020100400020623965 1101 8 0.07660561558483181 1101 9 0.08299430547641151 1101 10-11 0.07426943147098086 1101 12-13 0.06012639118026897 1101 14-15 0.18943346179206344 1101 16-17 0.11623440079252134 1101 18-19 0.14742368574810172 1101 20-21 0.1616481463669075 1101 22-23 0.1382523067072725 1101 24-25 0.17269492597615965 1101 26-27 0.20227559804829554 1101 28-29 0.22484973907273798 1101 30-31 0.25154195633720633 1101 32-33 0.2013549833621866 1101 34-35 0.20184792394631756 1101 36-37 0.10320745613268656 1101 38-39 0.08621894872483438 1101 40-41 0.22955127073488768 1101 42-43 0.16586781142689944 1101 44-45 0.1471433981018606 1101 46-47 0.19805107896917917 1101 48-49 0.2487364439279034 1101 50-51 0.30128269232851324 1101 52-53 0.2235543443743282 1101 54-55 0.24735242261035495 1101 56-57 0.2744837198321335 1101 58-59 0.2647121768970493 1101 60-61 0.2576405183359114 1101 62-63 0.2313222015385037 1101 64-65 0.30990234639452297 1101 66-67 0.2679781014948617 1101 68-69 0.22883205273210905 1101 70-71 0.2634951396428775 1101 72-73 0.22254393065736622 1101 74-75 0.3127489672159314 1101 76-77 0.35036066952905287 1101 78-79 0.32721271195043045 1101 80-81 0.3063528689219197 1101 82-83 0.2950508745055487 1101 84-85 0.2606304786641189 1101 86-87 0.31387291430502273 1101 88-89 0.2834749455109886 1101 90-91 0.3394728737056596 1101 92-93 0.3745325185244184 1101 94-95 0.3636090361129334 1101 96-97 0.33198551112509733 1101 98-99 0.3847229617032113 1101 100-101 0.3731635903258308 1101 102-103 0.4382969268524164 1101 104-105 0.43029334935363295 1101 106-107 0.43639361964900303 1101 108-109 0.3856991391500344 1101 110-111 0.4076150949487527 1101 112-113 0.3729344406708037 1101 114-115 0.4160410634364595 1101 116-117 0.48478239400870393 1101 118-119 0.4153564175795452 1101 120-121 0.39716812966600656 1101 122-123 0.3846260663381642 1101 124-125 0.47957272903062886 1102 1 0.07702584694167314 1102 2 0.16020514626116267 1102 3 0.17799091164825853 1102 4 0.0908859878265531 1102 5 -0.018640729045472426 1102 6 -0.0070833973911916814 1102 7 0.01614583858490448 1102 8 -0.04512186296480536 1102 9 0.0022369338440881847 1102 10-11 0.11669073694296372 1102 12-13 0.13101810174328676 1102 14-15 0.0898952939995894 1102 16-17 0.23147413765291702 1102 18-19 0.13055483136785284 1102 20-21 0.12940623257903638 1102 22-23 0.20686467360511784 1102 24-25 0.21230328645626884 1102 26-27 0.1500503405039879 1102 28-29 0.08042423946321264 1102 30-31 0.1469700975066459 1102 32-33 0.17728406676059194 1102 34-35 0.21958044500257046 1102 36-37 0.271887702927188 1102 38-39 0.20245017804065668 1102 40-41 0.20425265623151034 1102 42-43 0.21768470060894884 1102 44-45 0.2569988953363662 1102 46-47 0.2663709763351889 1102 48-49 0.24275015265936162 1102 50-51 0.25995866616390373 1102 52-53 0.31808967562719204 1102 54-55 0.28822270615022916 1102 56-57 0.2531617056919515 1102 58-59 0.3257055569217897 1102 60-61 0.25057146752399717 1102 62-63 0.2872930396296596 1102 64-65 0.26946091445539366 1102 66-67 0.26663009697713846 1102 68-69 0.18615224360478067 1102 70-71 0.26659070146958896 1102 72-73 0.23444106227799466 1102 74-75 0.16795554715812244 1102 76-77 0.2936192468203487 1102 78-79 0.23642036842939973 1102 80-81 0.2352895140059914 1102 82-83 0.26545752123865185 1102 84-85 0.25779264264825485 1102 86-87 0.2540094830566275 1102 88-89 0.21170452994497424 1102 90-91 0.2664579069403601 1102 92-93 0.2090958832930383 1102 94-95 0.19257373545529077 1102 96-97 0.20109165371125215 1102 98-99 0.21928969713133029 1102 100-101 0.20908221949417083 1102 102-103 0.22961291529053796 1102 104-105 0.26918049241375996 1102 106-107 0.3045526069162108 1102 108-109 0.2519900032108211 1102 110-111 0.2592467015710085 1102 112-113 0.2955160961935519 1102 114-115 0.25461966151176085 1102 116-117 0.2585618782044605 1102 118-119 0.2865121804220223 1102 120-121 0.23440235316458313 1102 122-123 0.262187637915936 1102 124-125 0.45011160932865835 1103 1 -0.022716673409952648 1103 2 0.04419988232195493 1103 3 0.011146205879192905 1103 4 -0.008717110354737656 1103 5 -0.10245301264019702 1103 6 -0.004895773265332082 1103 7 -0.11923258728863573 1103 8 -0.044224144673826515 1103 9 -2.109784342891885E-4 1103 10-11 -0.04967326324962329 1103 12-13 -0.02344611590775969 1103 14-15 -0.0966841686132014 1103 16-17 -0.03177397764018508 1103 18-19 -0.040450974211438506 1103 20-21 0.004626900762296771 1103 22-23 -0.07183422666489747 1103 24-25 0.046695576309943476 1103 26-27 0.031544003314976976 1103 28-29 0.014818084024540212 1103 30-31 -0.10404547765494243 1103 32-33 -0.0025579950024194886 1103 34-35 -0.010515034108173893 1103 36-37 -0.04868574346850352 1103 38-39 -0.07002964085095442 1103 40-41 -0.07914622558262607 1103 42-43 -0.12902137538917202 1103 44-45 -0.2013329334411651 1103 46-47 -0.21266855536680396 1103 48-49 -0.15531393539927052 1103 50-51 -0.14461087039278198 1103 52-53 -0.14254587883171155 1103 54-55 -0.12689928323362665 1103 56-57 -0.21867777425332235 1103 58-59 -0.2284654729566853 1103 60-61 -0.19507795893933633 1103 62-63 -0.1563834126678998 1103 64-65 -0.26764995726416885 1103 66-67 -0.2032697597891513 1103 68-69 -0.18483485533079147 1103 70-71 -0.16430780922078725 1103 72-73 -0.16779892038952937 1103 74-75 -0.22091119132600312 1103 76-77 -0.1410649770094068 1103 78-79 -0.17264804071347584 1103 80-81 -0.16436313421282023 1103 82-83 -0.13680101761700314 1103 84-85 -0.18095513562435173 1103 86-87 -0.17010774390661965 1103 88-89 -0.19239859431528572 1103 90-91 -0.12181277668967994 1103 92-93 -0.15800519878348496 1103 94-95 -0.08622371321929734 1103 96-97 -0.19126289586485967 1103 98-99 -0.2228844562049659 1103 100-101 -0.20106370235719595 1103 102-103 -0.21654354647569107 1103 104-105 -0.2263078462757946 1103 106-107 -0.25696015372300707 1103 108-109 -0.18330958393907082 1103 110-111 -0.13807187909123542 1103 112-113 -0.21328530444758798 1103 114-115 -0.18972305824365776 1103 116-117 -0.2599441580733952 1103 118-119 -0.16100164143054485 1103 120-121 -0.1912926513089559 1103 122-123 -0.23676042682409815 1103 124-125 -0.2297224281367889 1104 1 0.1203671637747803 1104 2 0.07605461510129885 1104 3 0.16447513113009649 1104 4 0.10372923712593973 1104 5 0.028726039124975955 1104 6 0.02210430771960148 1104 7 0.0709518904671711 1104 8 0.027981331883012217 1104 9 -0.07725505794174836 1104 10-11 0.08354460894035043 1104 12-13 0.09234481061916 1104 14-15 0.08434703626485884 1104 16-17 0.08395151898510278 1104 18-19 0.086615376153091 1104 20-21 0.16590815156473582 1104 22-23 0.1465376996111587 1104 24-25 0.1095009981525692 1104 26-27 0.11657843178848992 1104 28-29 0.11712699414509586 1104 30-31 0.10880095515620525 1104 32-33 0.061623800374327686 1104 34-35 0.07196059140168387 1104 36-37 0.15319081080029662 1104 38-39 -0.0032068230492754424 1104 40-41 0.024383507739912602 1104 42-43 0.06902197426624923 1104 44-45 0.007748440616396124 1104 46-47 0.0020550983574096904 1104 48-49 0.041535627183314716 1104 50-51 -0.004749147230896256 1104 52-53 -0.067939124885676 1104 54-55 -0.06901381963438524 1104 56-57 -0.007600541291267859 1104 58-59 0.017032949812332276 1104 60-61 -0.017880452485229625 1104 62-63 0.01283115197881557 1104 64-65 0.05577547832614016 1104 66-67 -0.026679640346252143 1104 68-69 -0.07550334152484339 1104 70-71 -0.02568789621045653 1104 72-73 -0.04938808183990773 1104 74-75 -0.0859358472881766 1104 76-77 -0.0991157683902415 1104 78-79 -0.06261037778067902 1104 80-81 -0.056932914585622996 1104 82-83 -0.024820333511584636 1104 84-85 -0.061993081016801455 1104 86-87 0.011605166474666362 1104 88-89 -0.020528563284230472 1104 90-91 0.028738217153495782 1104 92-93 0.0030335910250514075 1104 94-95 -0.031106428268870445 1104 96-97 0.05911017802697671 1104 98-99 0.053788351819250124 1104 100-101 0.047472643088987354 1104 102-103 0.02731320302605411 1104 104-105 0.06394413538605903 1104 106-107 0.03961038119236804 1104 108-109 -0.003374144734067386 1104 110-111 -0.06013597767953627 1104 112-113 -0.04176180498979676 1104 114-115 -0.029250298810389097 1104 116-117 -0.008440504862733178 1104 118-119 -0.0015695877908683542 1104 120-121 -0.10515825977248028 1104 122-123 -0.06245109498931356 1104 124-125 0.06615232767388335 1105 1 -0.0834195036170371 1105 2 -0.019488556869369233 1105 3 -0.11373185289452437 1105 4 0.019650808762136762 1105 5 -0.10702385756607669 1105 6 0.0066730347543000335 1105 7 -0.060495449649188515 1105 8 -0.02546763301234023 1105 9 -0.024725554800717475 1105 10-11 -0.011131787624215406 1105 12-13 0.03048848847588914 1105 14-15 -0.05409204933322087 1105 16-17 -0.02602127834920509 1105 18-19 0.013551429494008005 1105 20-21 0.012671607342440439 1105 22-23 0.013757783355742959 1105 24-25 -0.11156047880358244 1105 26-27 -0.03873072539018807 1105 28-29 0.022742800438031452 1105 30-31 -0.10181005109478036 1105 32-33 -0.051967116596358665 1105 34-35 0.03502928226004798 1105 36-37 0.008735648339389002 1105 38-39 0.05887857545132391 1105 40-41 0.11735840233079387 1105 42-43 0.042157035355700145 1105 44-45 -2.020992851399228E-4 1105 46-47 0.14184873297249112 1105 48-49 0.13606974632826763 1105 50-51 0.09412224806384017 1105 52-53 0.13748361143444754 1105 54-55 0.021847551172136548 1105 56-57 0.08237535895332826 1105 58-59 0.025032776942836676 1105 60-61 0.11139448624564352 1105 62-63 0.11668201416540569 1105 64-65 0.15085964780234207 1105 66-67 0.17186340002994882 1105 68-69 0.09369036796798014 1105 70-71 0.2548224766874867 1105 72-73 0.20639187763288191 1105 74-75 0.21217941393171458 1105 76-77 0.119316611231163 1105 78-79 0.11245713115535949 1105 80-81 0.0694952617820519 1105 82-83 0.17625869261161853 1105 84-85 -0.03579898256763414 1105 86-87 -0.025287416987069378 1105 88-89 0.01736323983683974 1105 90-91 0.07584126922266421 1105 92-93 -0.018261041231518504 1105 94-95 0.002269766339452417 1105 96-97 -0.05995999776090599 1105 98-99 -0.07250531654421621 1105 100-101 -0.01863767678270989 1105 102-103 -0.09652460168677379 1105 104-105 0.016449820412987037 1105 106-107 0.014707826657136991 1105 108-109 0.06125525172317481 1105 110-111 0.04460241881995586 1105 112-113 -0.028268515579554787 1105 114-115 0.07012028142411708 1105 116-117 -0.06709849859572614 1105 118-119 0.04002846927257053 1105 120-121 -0.14237958662353378 1105 122-123 -0.09456403121163959 1105 124-125 -0.07290454784600087 1106 1 -0.07547675480275728 1106 2 -0.0023746786228144856 1106 3 -0.07116583474114435 1106 4 -0.07119535964873336 1106 5 -0.12758327916360201 1106 6 -0.016460492907057755 1106 7 -0.15750455906739091 1106 8 -0.0688041064496332 1106 9 -0.0699865301653162 1106 10-11 -0.04938479896832604 1106 12-13 -0.05487671808139538 1106 14-15 -0.24011165199708273 1106 16-17 -0.10645472957279623 1106 18-19 -0.13623585378622494 1106 20-21 -0.05331674232645156 1106 22-23 -0.013245669200244947 1106 24-25 -0.0634591508434923 1106 26-27 0.006391867229247339 1106 28-29 -0.003582062406437103 1106 30-31 -0.11915663208257143 1106 32-33 -0.11676869397264511 1106 34-35 0.028346821571723524 1106 36-37 -0.022613302920426293 1106 38-39 -0.07923137805487812 1106 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16-17 -0.24202656728162708 2216 18-19 -0.3058477869604417 2216 20-21 -0.27585278051260076 2216 22-23 -0.3163258601310375 2216 24-25 -0.2656004396263114 2216 26-27 -0.3026735265654992 2216 28-29 -0.43737394784796635 2216 30-31 -0.48890603542386657 2216 32-33 -0.5242822977912667 2216 34-35 -0.44198873928745996 2216 36-37 -0.36030685900635007 2216 38-39 -0.3420714735202708 2216 40-41 -0.26522710207664346 2216 42-43 -0.1925062359469365 2216 44-45 -0.2974516585407656 2216 46-47 -0.296800826283139 2216 48-49 -0.3173175210566441 2216 50-51 -0.4699335795561339 2216 52-53 -0.40061002225800735 2216 54-55 -0.457257052157928 2216 56-57 -0.5153690093130763 2216 58-59 -0.5241411701678373 2216 60-61 -0.4297034569473084 2216 62-63 -0.5224304246200973 2216 64-65 -0.43294955885779984 2216 66-67 -0.49008410241863487 2216 68-69 -0.5369611501515124 2216 70-71 -0.45480063790604675 2216 72-73 -0.47528099210266816 2216 74-75 -0.4570813108479186 2216 76-77 -0.4619590009137937 2216 78-79 -0.4182962478847969 2216 80-81 -0.4362794688741687 2216 82-83 -0.383485479768396 2216 84-85 -0.37625911968810044 2216 86-87 -0.370981050679525 2216 88-89 -0.3515260843551289 2216 90-91 -0.3278844898370892 2216 92-93 -0.3095119716918546 2216 94-95 -0.2905388526615269 2216 96-97 -0.35534178032701647 2216 98-99 -0.35127848010935026 2216 100-101 -0.40701828403050655 2216 102-103 -0.3363586859179222 2216 104-105 -0.38499151161443734 2216 106-107 -0.32263027324491844 2216 108-109 -0.3540450421063994 2216 110-111 -0.3355037516011947 2216 112-113 -0.30402992596153666 2216 114-115 -0.4198613052556084 2216 116-117 -0.5496254329737909 2216 118-119 -0.622440286848871 2216 120-121 -0.5703962369663316 2216 122-123 -0.6112357256288803 2216 124-125 -0.7694132647901739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 133.0 3 3.0 4 10.0 5 30.0 6 78.0 7 214.0 8 368.0 9 717.0 10 1414.0 11 2282.0 12 2847.0 13 4516.0 14 7045.0 15 7671.0 16 3044.0 17 2274.0 18 2189.0 19 1939.0 20 1893.0 21 1905.0 22 2085.0 23 2341.0 24 2785.0 25 3628.0 26 4796.0 27 6140.0 28 8018.0 29 10450.0 30 13142.0 31 17090.0 32 22435.0 33 29627.0 34 42033.0 35 67105.0 36 148344.0 37 510883.0 38 464869.0 39 5488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.797432952105076 19.706052325539915 14.197672676422116 24.298842045932894 2 13.741314038568131 20.484851597660487 43.1972184949541 22.57661586881728 3 19.73098041062011 24.93988219692673 28.71458827775959 26.61454911469357 4 11.695418349287468 17.561603360176797 39.65264001152778 31.090338279007955 5 12.74020330499177 38.105258045346496 35.39695328213568 13.757585367526056 6 29.805732645375944 38.022414970135486 19.076693267590745 13.095159116897829 7 27.473140485550683 31.803619694528084 22.38343994390194 18.339799876019292 8 23.526516391776184 38.33928626203872 20.765555905098402 17.368641441086694 9 25.108090775564246 17.463945368592935 21.824028716728336 35.60393513911448 10-11 23.762279475914 27.030826112420115 29.366878033086728 19.840016378579158 12-13 25.70555937199277 24.249927416357604 29.21989169878537 20.824621512864248 14-15 20.99400712354057 27.490474957395005 27.030576438957336 24.48494148010709 16-17 20.38274242142684 29.516478632759096 29.198073518874075 20.902705426939992 18-19 20.75296522904687 28.17525793052087 30.680873800051504 20.39090304038076 20-21 21.622298371951757 27.58943065216453 29.88128227837581 20.906988697507902 22-23 20.641185139953176 28.496546647016107 30.00786117535272 20.854407037677998 24-25 21.14887600573821 27.664390358038883 30.734553594548842 20.45218004167407 26-27 20.350206265947893 28.324419990712148 30.655906453773675 20.66946728956629 28-29 20.164270871453123 28.77557993795258 29.69741003016769 21.362739160426614 30-31 20.89995156334822 27.86088337324542 30.129880135337284 21.109284928069076 32-33 20.326273281158713 28.394471230840235 30.539094940830957 20.740160547170095 34-35 20.793447997654496 28.512602446371925 30.4039502621928 20.289999293780777 36-37 20.37249853941024 28.046569094277412 30.475642213647724 21.105290152664622 38-39 20.907227761406332 28.511247076145413 29.978007334692986 20.603517827755272 40-41 21.78030019310459 27.6822598444463 30.209561637601105 20.327878324848005 42-43 20.4055624394096 28.70238994572099 30.176426402326673 20.71562121254274 44-45 20.510282623226335 28.648210804298092 29.729974583241493 21.11153198923408 46-47 21.417560319325226 28.115264964178994 29.404792731791492 21.062381984704288 48-49 21.19834701900586 28.384091948316165 29.150446808495463 21.267114224182514 50-51 20.562785385684865 29.233231395225246 29.207622031471697 20.99636118761819 52-53 20.781784680178994 28.95823390979369 29.349828902342722 20.910152507684593 54-55 20.644749616751234 28.308119880356475 29.739569177739682 21.307561325152605 56-57 21.04611754198616 28.416870507215208 28.81855944118799 21.718452509610646 58-59 21.393841340361284 28.64425169653118 28.52922356546545 21.432683397642084 60-61 20.86121650898004 28.07210712275588 29.92183080556786 21.144845562696215 62-63 21.056104480497293 28.07178611401802 29.7733107628523 21.098798642632385 64-65 21.043299798620517 28.640791935689826 29.067804892315834 21.248103373373823 66-67 21.05057599667863 29.053002822736833 28.557151325659085 21.33926985492545 68-69 21.42276779440603 28.11965208359638 29.47277524894228 20.98480487305531 70-71 21.908917694072965 27.983829719844973 29.084675684872142 21.02257690120992 72-73 21.375793515766166 28.47047896643747 29.09284357386875 21.060883943927617 74-75 20.73930452386914 28.99440089425901 29.519892198132304 20.74640238373955 76-77 21.09829929570683 28.05940944379173 29.65917432272506 21.18311693777638 78-79 20.945820145224353 28.089584265150364 29.373298207843884 21.5912973817814 80-81 21.305528269812836 28.470871310450402 29.18219100590585 21.041409413830912 82-83 21.049933979202915 28.489061805595682 29.720629662206072 20.740374552995334 84-85 21.018261117067606 28.405278525014783 29.613769420136947 20.96269093778066 86-87 20.412624631642473 29.21471989134211 28.84730755704504 21.52534791997038 88-89 21.003280709300906 28.345891908511085 29.056462583578192 21.59436479860982 90-91 21.027465029365416 28.381873836779214 29.45094908829171 21.13971204556366 92-93 20.82137575784813 28.970182568369513 29.45447775088438 20.75396392289798 94-95 20.65308460509857 28.86634525220525 29.278807725218098 21.20176241747808 96-97 21.105147482114464 28.440910494917006 29.27307214635716 21.180869876611375 98-99 21.176114745894683 28.322444438816802 29.376010523533676 21.12543029175484 100-101 20.751303653082044 28.288736865383107 29.55315551814414 21.40680396339071 102-103 20.718854751691897 28.63431079584 29.249400960318212 21.397433492149887 104-105 21.09157200293336 28.185324053862022 29.122544949222572 21.60055899398204 106-107 20.86981769826402 28.008613567974706 29.278497754936357 21.84307097882492 108-109 20.50053983142707 28.531562215438218 28.96820683586735 21.999691117267364 110-111 20.379255279116062 28.809326185721158 29.23432351945589 21.577095015706888 112-113 20.82841671957823 28.152328227664782 28.90523004045059 22.114025012306396 114-115 20.52190612481189 28.356775794524836 28.59683021161528 22.524487869047995 116-117 21.001874001739175 28.34261773859994 28.095901521814966 22.559606737845918 118-119 20.864527699170154 27.88407145236687 28.955421809650105 22.295979038812867 120-121 20.55666819443974 28.189160895905154 29.143097646099985 22.111073263555124 122-123 20.826969568781088 28.29014058183058 28.747796609685857 22.13509323970247 124-125 21.05981212207464 28.23836534719822 28.41099874484106 22.29082378588608 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 73.0 1 49.5 2 67.5 3 93.5 4 53.0 5 42.0 6 121.0 7 285.0 8 519.0 9 589.5 10 442.0 11 346.5 12 370.5 13 455.0 14 546.0 15 663.0 16 785.0 17 950.5 18 1171.0 19 1429.0 20 1730.5 21 2219.0 22 2985.5 23 3833.0 24 4883.5 25 6471.5 26 8428.5 27 10674.0 28 13445.0 29 16562.5 30 20217.5 31 24541.5 32 30028.5 33 36820.0 34 44273.5 35 51598.0 36 57603.5 37 60563.5 38 59486.5 39 56269.5 40 54128.5 41 53472.5 42 52224.0 43 51758.5 44 52294.0 45 52625.0 46 52619.5 47 54199.5 48 58438.5 49 56187.5 50 48825.0 51 43797.0 52 39643.5 53 36218.0 54 33179.0 55 28989.0 56 23991.5 57 20093.5 58 16741.5 59 13301.5 60 10257.5 61 7887.0 62 5943.0 63 4071.5 64 2700.0 65 1960.5 66 1380.5 67 968.0 68 667.5 69 420.5 70 320.0 71 246.0 72 188.0 73 151.0 74 120.0 75 78.5 76 34.5 77 17.0 78 12.0 79 5.5 80 2.0 81 2.5 82 2.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 2.5 98 5.5 99 4.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015765095792574138 2 0.0 3 0.0 4 0.0 5 0.12604943106551358 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.56676375397605E-5 18-19 0.0 20-21 0.00431578414231102 22-23 3.56676375397605E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.560233009542519E-4 92-93 0.0 94-95 4.6367928801688653E-4 96-97 0.0 98-99 0.0014623731391301806 100-101 7.1335275079521E-5 102-103 2.85341100318084E-4 104-105 0.001926052427147067 106-107 0.006348839482077369 108-109 3.210087378578445E-4 110-111 0.004101778317072457 112-113 0.00863156828462204 114-115 0.004672460517708626 116-117 0.001284034951431378 118-119 1.42670550159042E-4 120-121 6.063498381759285E-4 122-123 9.98693851113294E-4 124-125 0.0011770320388120965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1401831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.51878511887655 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01848909639338 55.627881490293376 2 12.843304073475387 17.857017922006545 3 3.7012559399346556 7.719204490748482 4 1.5133731110767112 4.208314404545104 5 0.7046471967389887 2.4493108527358243 6 0.385558977248741 1.6082155014005424 7 0.21571039831985175 1.0497147380093528 8 0.12965410652733983 0.7210716777163253 9 0.10562557638483717 0.6608665572979479 >10 0.35263235161843126 3.992122853013841 >50 0.01528050820038054 0.7331999343523341 >100 0.012211207162913856 1.5851543765004399 >500 0.0012313401373110799 0.5827306483431477 >1k 0.0010261167810925664 1.2051945530367831 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3629 0.2588757132635817 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2443 0.1742720770192698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1722 0.12283934368693517 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1670 0.11912990938280008 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1554 0.11085501747357562 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.56676375397605E-5 0.0 16-17 0.0 0.0 0.0 7.1335275079521E-5 0.0 18-19 0.0 0.0 0.0 1.42670550159042E-4 0.0 20-21 0.0 0.0 0.0 2.14005825238563E-4 0.0 22-23 0.0 0.0 0.0 4.28011650477126E-4 0.0 24-25 0.0 0.0 0.0 7.1335275079521E-4 0.0 26-27 0.0 0.0 0.0 8.56023300954252E-4 0.0 28-29 0.0 0.0 0.0 0.0013553702265108989 0.0 30-31 0.0 0.0 0.0 0.002817743365641079 0.0 32-33 0.0 0.0 0.0 0.007490203883349705 0.0 34-35 0.0 0.0 0.0 0.013839043365427073 0.0 36-37 0.0 0.0 0.0 0.02361197605132145 0.0 38-39 0.0 0.0 0.0 0.040411433332548645 0.0 40-41 0.0 0.0 0.0 0.05999296634187716 0.0 42-43 0.0 0.0 0.0 0.08110820776541539 0.0 44-45 0.0 0.0 0.0 0.1060042187681682 0.0 46-47 0.0 0.0 0.0 0.13464533171259588 0.0 48-49 0.0 0.0 0.0 0.1658545145598863 0.0 50-51 0.0 0.0 0.0 0.20448256601544695 0.0 52-53 0.0 0.0 0.0 0.2398291948173496 0.0 54-55 0.0 0.0 0.0 0.2802049605123585 7.1335275079521E-5 56-57 0.0 0.0 0.0 0.32964030614246653 7.1335275079521E-5 58-59 0.0 0.0 0.0 0.38253541261393137 7.1335275079521E-5 60-61 0.0 0.0 0.0 0.4434200698943025 7.1335275079521E-5 62-63 0.0 0.0 0.0 0.5028780216730833 7.1335275079521E-5 64-65 0.0 0.0 0.0 0.5670797692446521 7.1335275079521E-5 66-67 0.0 0.0 0.0 0.6410187818645756 7.1335275079521E-5 68-69 0.0 0.0 0.0 0.7272274617981769 7.1335275079521E-5 70-71 0.0 0.0 0.0 0.8262764912460917 7.1335275079521E-5 72-73 0.0 0.0 0.0 0.9215090834772522 7.1335275079521E-5 74-75 0.0 0.0 0.0 1.0233401886532685 7.1335275079521E-5 76-77 0.0 0.0 0.0 1.1312704598485839 7.1335275079521E-5 78-79 0.0 0.0 0.0 1.2655234475482422 7.1335275079521E-5 80-81 0.0 0.0 0.0 1.4026655138886213 7.1335275079521E-5 82-83 0.0 0.0 0.0 1.5508288802287864 7.1335275079521E-5 84-85 0.0 0.0 0.0 1.6982788938181563 7.1335275079521E-5 86-87 0.0 0.0 0.0 1.852042079252064 7.1335275079521E-5 88-89 0.0 0.0 0.0 2.025850476983317 7.1335275079521E-5 90-91 0.0 0.0 0.0 2.215851982157621 7.1335275079521E-5 92-93 0.0 0.0 0.0 2.4247930028655382 7.1335275079521E-5 94-95 0.0 0.0 0.0 2.6282768750298713 7.1335275079521E-5 96-97 0.0 0.0 0.0 2.8369682222750106 7.1335275079521E-5 98-99 0.0 0.0 0.0 3.061281994762564 7.1335275079521E-5 100-101 0.0 0.0 0.0 3.315342576958278 7.1335275079521E-5 102-103 0.0 0.0 0.0 3.5884140099626847 7.1335275079521E-5 104-105 0.0 0.0 0.0 3.8629121484686815 7.1335275079521E-5 106-107 0.0 7.1335275079521E-5 0.0 4.1409770507286545 7.1335275079521E-5 108-109 0.0 7.1335275079521E-5 0.0 4.432345981790958 7.1335275079521E-5 110-111 0.0 7.1335275079521E-5 0.0 4.753354719648803 7.1335275079521E-5 112-113 0.0 7.1335275079521E-5 0.0 5.106036319641953 7.1335275079521E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4305 0.0 30.172762 1 TACATGG 4345 0.0 29.340172 2 TAGGACC 1405 0.0 27.559776 4 ACATGGG 4115 0.0 27.360916 3 GGTCCGT 90 0.00953355 26.442232 6 GTGTAGC 1045 0.0 26.228489 1 GTAGGAC 3495 0.0 25.05583 3 GTCCTAC 3585 0.0 24.265844 1 CATGGGG 2130 0.0 24.05235 4 TCCTACA 4190 0.0 23.885479 2 CTACAGT 4180 0.0 23.515072 4 GAGTACT 3995 0.0 23.455484 12-13 TGTAGGA 4100 0.0 23.39272 2 CCTACAG 4220 0.0 22.30403 3 AGAGTAC 5940 0.0 22.235514 10-11 GTATCAA 8980 0.0 21.962584 1 GTACTTT 4275 0.0 21.850803 14-15 TAGCCCT 1450 0.0 21.774416 4 AGTGGAC 2705 0.0 21.33463 8 GACGTGG 1870 0.0 21.316399 7 >>END_MODULE