FastQCFastQC Report
Fri 27 May 2016
SRR939920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428407
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA29350.20547365001711698No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC28220.19756273947131314No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23140.1619986460441597No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA18140.12699461708042595No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16080.11257295714736766No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA15570.10900254619306683No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14540.10179171622653768No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA14360.10053157118384325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC300.03370.4531108-109
CGTCGCT207.5731805E-73058.6863110-111
CCGCTCC1950.02592.6562108-109
CCGCTCG500.02527.8398108-109
CCGCGCC1650.02451.2388108-109
CGTCTCG401.9645086E-92294.015110-111
CCGCACC950.02128.707108-109
CGCGGTT150.00196460052039.124110-111
CGCTTCG150.00196460052039.124110-111
CAGCGCG750.02022.2719108-109
CCGCGCG700.01805.6108-109
CGACGCT150.00287742521685.2266108-109
CCTCGCG900.01685.2266108-109
AACGGCG150.00287742521685.2266108-109
CCGCGCT150.00287742521685.2266108-109
CCGCGAC150.00287742521685.2266108-109
CGTCGCC1150.01538.685108-109
CTGCGCT1000.01529.3431110-111
CTCCGCC1350.01359.4161110-111
CGCCGCC1150.01329.8635110-111
Produced by FastQC (version 0.11.5)