FastQCFastQC Report
Fri 27 May 2016
SRR939920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428407
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA28740.20120315848354145No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC27610.1932922479377376No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26100.18272103119069003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.1628387427392893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.1292348749341049No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA17430.12202404496757577No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16410.1148832230589741No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16040.11229292491565779No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA15270.1069023044552428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG853.4465975E-642.061561
TAGGACC24000.036.9859474
ATAACGC700.002772112834.042673
GTACATG56900.033.197091
TACATGG55450.032.3390052
ACATGGG54150.030.2549173
GTGTAGC10450.027.9404141
CATGGGG28350.027.5283344
GTATAGC7400.026.5726761
TAGCCCT12500.026.212864
GTAGCCC8700.026.0211243
GAGTACT42200.024.74190312-13
GTAGGAC81750.024.5586153
GACCTGG55750.024.2241977
AGTACTT42150.023.78322612-13
GGACCTG55950.023.6059386
GTACTTT44550.023.5723814-15
TGTAGGA88750.023.3599872
ACCTGGA61600.022.6963278
GTATCAA105950.022.2713891
Produced by FastQC (version 0.11.5)