FastQCFastQC Report
Fri 27 May 2016
SRR939919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607459
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34750.5720550687371494No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20610.3392821573143208No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15180.2498934084440267No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10330.17005262906632382No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9730.16017541924640183No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9580.15770611679142132No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9200.15145055057213738No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9120.15013358926281445No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9100.14980434893548372No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8530.14042099960655782No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7440.12247740176703284No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6470.10650924589149227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG100.00134014992466.2014108-109
CGCGAGT200.00339366471548.7158110-111
CTGCGCG458.215657E-61376.6364110-111
CGTATGA250.00530203761238.9727110-111
GTGCGAT250.00530203761238.9727110-111
CAGCGCG401.1472099E-51233.1007108-109
CGCGTTC300.00763412331032.4772110-111
CCGCTCC2200.01008.9007108-109
CCGCGCT859.336327E-8870.4241108-109
CGTGGCG603.8695136E-5822.06714108-109
CCGTGCC1001.7877028E-7739.8604108-109
CATCGCG757.554246E-5657.6537108-109
CCGCGCC5150.0574.6489108-109
CCACGCC1601.1693501E-6462.41278108-109
CGGCGCC1109.560354E-7448.4003108-109
CCTCGCC3108.185452E-11397.7744108-109
CTCCGCC1603.6827914E-4387.17896110-111
CGCTGCT1601.638835E-5387.17896110-111
TTTTTCG3500.0375.505592-93
TTTCGCG353.5590474E-6352.036492-93
Produced by FastQC (version 0.11.5)