##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR939919_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607459
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10834476071636	34.0	31.0	34.0	30.0	34.0
2	32.13870565750116	34.0	31.0	34.0	30.0	34.0
3	32.23492943556685	34.0	31.0	34.0	30.0	34.0
4	35.46900449248427	37.0	35.0	37.0	35.0	37.0
5	35.49074752370119	37.0	37.0	37.0	35.0	37.0
6	35.61283971428524	37.0	37.0	37.0	35.0	37.0
7	35.55662357459516	37.0	37.0	37.0	35.0	37.0
8	35.55418554997127	37.0	37.0	37.0	35.0	37.0
9	37.256435413748086	39.0	39.0	39.0	35.0	39.0
10-11	37.239469659680736	39.0	39.0	39.0	35.0	39.0
12-13	37.1930829899631	39.0	39.0	39.0	35.0	39.0
14-15	38.74595980963325	41.0	40.0	41.0	36.0	41.0
16-17	38.7525330927684	41.0	40.0	41.0	36.0	41.0
18-19	38.77972340520101	41.0	40.0	41.0	36.0	41.0
20-21	38.83001321899914	41.0	40.0	41.0	35.5	41.0
22-23	38.80458598851939	41.0	39.5	41.0	35.0	41.0
24-25	38.81625261951835	41.0	39.5	41.0	35.0	41.0
26-27	38.77158623051103	41.0	39.0	41.0	35.0	41.0
28-29	38.70562375403114	41.0	39.0	41.0	35.0	41.0
30-31	38.63270772183802	41.0	39.0	41.0	34.5	41.0
32-33	38.502401808187884	41.0	39.0	41.0	34.0	41.0
34-35	38.38119609718516	40.5	39.0	41.0	34.0	41.0
36-37	38.52946371689283	41.0	39.0	41.0	34.0	41.0
38-39	38.4977858587987	41.0	39.0	41.0	34.0	41.0
40-41	38.44307681670697	41.0	39.0	41.0	34.0	41.0
42-43	38.31533074660183	41.0	39.0	41.0	34.0	41.0
44-45	38.174169779359595	41.0	38.0	41.0	33.0	41.0
46-47	38.045157780195865	40.5	38.0	41.0	33.0	41.0
48-49	37.90850740543806	40.0	38.0	41.0	33.0	41.0
50-51	37.74860114015925	40.0	38.0	41.0	33.0	41.0
52-53	37.6017937013033	40.0	37.0	41.0	33.0	41.0
54-55	37.39900799889375	40.0	37.0	41.0	32.0	41.0
56-57	37.16991599433048	40.0	36.5	41.0	32.0	41.0
58-59	36.958949492887584	40.0	36.0	41.0	31.5	41.0
60-61	36.665270413311845	39.0	35.0	41.0	31.0	41.0
62-63	36.39080662233994	39.0	35.0	41.0	31.0	41.0
64-65	36.06813217023701	39.0	35.0	41.0	31.0	41.0
66-67	35.710929461906076	37.5	35.0	40.5	30.5	41.0
68-69	35.32067925571931	37.0	35.0	40.0	30.0	41.0
70-71	34.90422810428358	36.5	35.0	39.0	30.0	41.0
72-73	34.488196734265195	36.0	35.0	39.0	29.0	41.0
74-75	34.091810311477815	35.5	34.0	38.5	29.0	40.5
76-77	33.6766580131334	35.0	34.0	37.0	29.0	39.0
78-79	33.246276703448295	35.0	34.0	37.0	28.5	39.0
80-81	32.857078420107364	35.0	34.0	36.5	27.0	39.0
82-83	32.72379205839407	35.0	34.0	36.0	28.5	37.0
84-85	32.41242865773657	35.0	34.0	36.0	28.5	37.0
86-87	31.850836846601993	35.0	34.0	35.0	25.5	37.0
88-89	16.62006736257097	18.0	17.0	18.5	2.0	19.0
90-91	2.888686314631934	2.0	2.0	2.0	2.0	2.0
92-93	2.323982359303262	2.0	2.0	2.0	2.0	2.0
94-95	2.1077891676639906	2.0	2.0	2.0	2.0	2.0
96-97	2.0	2.0	2.0	2.0	2.0	2.0
98-99	2.0	2.0	2.0	2.0	2.0	2.0
100-101	2.0	2.0	2.0	2.0	2.0	2.0
102-103	2.0	2.0	2.0	2.0	2.0	2.0
104-105	2.0	2.0	2.0	2.0	2.0	2.0
106-107	2.0	2.0	2.0	2.0	2.0	2.0
108-109	2.0	2.0	2.0	2.0	2.0	2.0
110-111	2.0	2.0	2.0	2.0	2.0	2.0
112-113	2.0	2.0	2.0	2.0	2.0	2.0
114-115	2.0	2.0	2.0	2.0	2.0	2.0
116-117	2.0	2.0	2.0	2.0	2.0	2.0
118-119	2.0	2.0	2.0	2.0	2.0	2.0
120-121	2.0	2.0	2.0	2.0	2.0	2.0
122-123	2.0	2.0	2.0	2.0	2.0	2.0
124-125	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3265594294010796
1101	2	0.2835034118867483
1101	3	0.2596252681320408
1101	4	0.27752819948138097
1101	5	0.36887474544835186
1101	6	0.20393427180942325
1101	7	0.4056108833480039
1101	8	0.33869959549669915
1101	9	0.2521908119609009
1101	10-11	0.28395089986107536
1101	12-13	0.34034111393308564
1101	14-15	0.48657315669139223
1101	16-17	0.46200604948045765
1101	18-19	0.5152451599278436
1101	20-21	0.4777634025572155
1101	22-23	0.6017078338748405
1101	24-25	0.437321152035544
1101	26-27	0.498617597092597
1101	28-29	0.6518353180719458
1101	30-31	0.5629975928569166
1101	32-33	0.5998475684750488
1101	34-35	0.6132112468466886
1101	36-37	0.6091528012702057
1101	38-39	0.5689017644788805
1101	40-41	0.529670025266114
1101	42-43	0.5546654486477394
1101	44-45	0.568057710855804
1101	46-47	0.6607240610263929
1101	48-49	0.6575714903235621
1101	50-51	0.5542039487750259
1101	52-53	0.5916102565399584
1101	54-55	0.7430307227585118
1101	56-57	0.7171056650678977
1101	58-59	0.6444242373827791
1101	60-61	0.6994228541722762
1101	62-63	0.7100841403442999
1101	64-65	0.6503968016222714
1101	66-67	0.7785873926402829
1101	68-69	0.7241827331887407
1101	70-71	0.7225193692133587
1101	72-73	0.9162020113891529
1101	74-75	0.9486024026475448
1101	76-77	0.8002528387862498
1101	78-79	0.8649679093805105
1101	80-81	0.8457542781834277
1101	82-83	0.853987466490679
1101	84-85	0.8959767318064564
1101	86-87	0.9228931041457002
1101	88-89	1.1812543634672537
1101	90-91	-0.17825578268120879
1101	92-93	-0.23960319714565426
1101	94-95	-0.04339549183898184
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
1101	102-103	0.0
1101	104-105	0.0
1101	106-107	0.0
1101	108-109	0.0
1101	110-111	0.0
1101	112-113	0.0
1101	114-115	0.0
1101	116-117	0.0
1101	118-119	0.0
1101	120-121	0.0
1101	122-123	0.0
1101	124-125	0.0
1102	1	0.08942593226758788
1102	2	0.0743961227794614
1102	3	0.15261462112139412
1102	4	0.12593114788432302
1102	5	0.13216712874073977
1102	6	-0.03821151033636028
1102	7	0.12628246401958876
1102	8	0.08931384611094728
1102	9	0.19938163915173135
1102	10-11	0.22562985154002746
1102	12-13	0.1697186733106406
1102	14-15	0.09136431148254331
1102	16-17	0.24392251139691723
1102	18-19	0.18899618367886717
1102	20-21	0.2368706916645067
1102	22-23	0.2074408396078553
1102	24-25	0.27346365817805207
1102	26-27	0.26085346932846676
1102	28-29	0.3546117308483545
1102	30-31	0.32317777303708795
1102	32-33	0.3049171835446671
1102	34-35	0.38580747944291716
1102	36-37	0.3493985015159069
1102	38-39	0.3987707243478411
1102	40-41	0.3097601153562124
1102	42-43	0.35308477706706753
1102	44-45	0.37245574525384484
1102	46-47	0.38307459337692507
1102	48-49	0.4240825468346259
1102	50-51	0.4183972329683101
1102	52-53	0.5687519436816402
1102	54-55	0.4704099201377119
1102	56-57	0.5405700385322731
1102	58-59	0.5786093315678613
1102	60-61	0.4956226903721088
1102	62-63	0.39286055312071966
1102	64-65	0.5164164576419239
1102	66-67	0.5368265408794315
1102	68-69	0.6820615210675385
1102	70-71	0.6543948810888764
1102	72-73	0.6048916100787451
1102	74-75	0.603377177422324
1102	76-77	0.5325542310876443
1102	78-79	0.3495625039751076
1102	80-81	0.3436084960376391
1102	82-83	0.36294733545054214
1102	84-85	0.4372298030595303
1102	86-87	0.8389045701571689
1102	88-89	1.5791577213706134
1102	90-91	0.17212914770372123
1102	92-93	0.08811179056933316
1102	94-95	0.18350868506519502
1102	96-97	0.0
1102	98-99	0.0
1102	100-101	0.0
1102	102-103	0.0
1102	104-105	0.0
1102	106-107	0.0
1102	108-109	0.0
1102	110-111	0.0
1102	112-113	0.0
1102	114-115	0.0
1102	116-117	0.0
1102	118-119	0.0
1102	120-121	0.0
1102	122-123	0.0
1102	124-125	0.0
1103	1	0.17240441063798784
1103	2	0.34468043862197106
1103	3	0.30422190536525306
1103	4	0.30420797794263166
1103	5	0.24579701429461664
1103	6	0.3187509887639095
1103	7	0.25526684464292515
1103	8	0.31518298884470397
1103	9	0.413506738122436
1103	10-11	0.35901932825492366
1103	12-13	0.24275113570177353
1103	14-15	0.25618832357496046
1103	16-17	0.329396491098926
1103	18-19	0.41722622758587136
1103	20-21	0.371185814388177
1103	22-23	0.38392899911264067
1103	24-25	0.38111211423505864
1103	26-27	0.45733587053771174
1103	28-29	0.3716188567390901
1103	30-31	0.38044114307956534
1103	32-33	0.4762358332243579
1103	34-35	0.5271038157273793
1103	36-37	0.4454922177758931
1103	38-39	0.5030923251540003
1103	40-41	0.4773682633444025
1103	42-43	0.43627703215956615
1103	44-45	0.23962914192842533
1103	46-47	0.26257002272983954
1103	48-49	0.43180010243816014
1103	50-51	0.3342044740249577
1103	52-53	0.38171746588707123
1103	54-55	0.27854783131599703
1103	56-57	0.32691208128761673
1103	58-59	0.23702580029312514
1103	60-61	0.17774462228026522
1103	62-63	0.1723099161092776
1103	64-65	0.15909406507012847
1103	66-67	0.3194343289741468
1103	68-69	0.3825718500101658
1103	70-71	0.36674570524492367
1103	72-73	0.4084531158730371
1103	74-75	0.45063191002147107
1103	76-77	0.32893586212485815
1103	78-79	0.39370199868467637
1103	80-81	0.46748094979133015
1103	82-83	0.4263771202342497
1103	84-85	0.3567245126254974
1103	86-87	0.2374973866834722
1103	88-89	1.2198602334270454
1103	90-91	-0.5781270964480099
1103	92-93	-0.3427973003397575
1103	94-95	-0.1164913149348048
1103	96-97	0.0
1103	98-99	0.0
1103	100-101	0.0
1103	102-103	0.0
1103	104-105	0.0
1103	106-107	0.0
1103	108-109	0.0
1103	110-111	0.0
1103	112-113	0.0
1103	114-115	0.0
1103	116-117	0.0
1103	118-119	0.0
1103	120-121	0.0
1103	122-123	0.0
1103	124-125	0.0
1104	1	-0.04041834382669407
1104	2	-0.03769562806228777
1104	3	-0.055209452952681204
1104	4	0.10198333643651836
1104	5	-0.015614857791248937
1104	6	-0.005400062524913096
1104	7	-0.0428631588760382
1104	8	0.04670020974731415
1104	9	0.08748349100358155
1104	10-11	0.08832345423363108
1104	12-13	0.16129274738471366
1104	14-15	0.1745608603040978
1104	16-17	0.1987015518009585
1104	18-19	0.10917716010984435
1104	20-21	0.1540234694422864
1104	22-23	0.05030700122401299
1104	24-25	0.10492199151138237
1104	26-27	0.26184673532173974
1104	28-29	0.30072284195946963
1104	30-31	0.27663736899668834
1104	32-33	0.1846667626692451
1104	34-35	0.26805074543618446
1104	36-37	0.17929538703779002
1104	38-39	0.18912425970137292
1104	40-41	0.21961280895889956
1104	42-43	0.14865716763945613
1104	44-45	-0.05668777326467733
1104	46-47	0.05786836442069898
1104	48-49	0.03447396097603672
1104	50-51	0.06598561680669235
1104	52-53	0.1333394857691914
1104	54-55	0.15574030734309474
1104	56-57	0.025755223717453646
1104	58-59	-0.029330483246951644
1104	60-61	0.002057875557291311
1104	62-63	-0.1252455074853387
1104	64-65	-0.1456037443782776
1104	66-67	-0.06691293723505254
1104	68-69	-0.161391761760747
1104	70-71	-0.2421413141973261
1104	72-73	-0.19859744716031003
1104	74-75	-0.15604201449686883
1104	76-77	-0.11184391706050434
1104	78-79	0.0452632615508719
1104	80-81	0.0088946913238388
1104	82-83	0.008195651948859961
1104	84-85	0.0661355269652546
1104	86-87	0.14605945231205197
1104	88-89	1.083507468845358
1104	90-91	-0.7078350778855045
1104	92-93	-0.3427973003397575
1104	94-95	-0.1164913149348048
1104	96-97	0.0
1104	98-99	0.0
1104	100-101	0.0
1104	102-103	0.0
1104	104-105	0.0
1104	106-107	0.0
1104	108-109	0.0
1104	110-111	0.0
1104	112-113	0.0
1104	114-115	0.0
1104	116-117	0.0
1104	118-119	0.0
1104	120-121	0.0
1104	122-123	0.0
1104	124-125	0.0
1105	1	0.1574256604915263
1105	2	0.11085216300231338
1105	3	0.21537994249658254
1105	4	0.2257273158419295
1105	5	0.2020244463110572
1105	6	0.23062800590226118
1105	7	0.26875970277485095
1105	8	0.1379277882226475
1105	9	0.278559516580728
1105	10-11	0.17597500713181802
1105	12-13	0.2614526045513017
1105	14-15	0.18437287242832667
1105	16-17	0.13896531612581953
1105	18-19	0.2681883293508278
1105	20-21	0.24968085608902157
1105	22-23	0.2034117595698035
1105	24-25	0.3728358554874731
1105	26-27	0.37717226264278025
1105	28-29	0.2938718205344557
1105	30-31	0.2551189335208619
1105	32-33	0.3640882665722529
1105	34-35	0.4027615492893659
1105	36-37	0.24794314073319157
1105	38-39	0.2712119527756158
1105	40-41	0.4215674261321283
1105	42-43	0.4549185860085032
1105	44-45	0.3535768214870245
1105	46-47	0.49411753399385816
1105	48-49	0.46255380651663813
1105	50-51	0.49976426653265804
1105	52-53	0.5493729519708168
1105	54-55	0.4935761111962762
1105	56-57	0.5647852851690445
1105	58-59	0.5097229339594946
1105	60-61	0.6284851716875082
1105	62-63	0.5026506061170508
1105	64-65	0.6538481739077255
1105	66-67	0.7050233067443585
1105	68-69	0.7670384201027289
1105	70-71	0.7852679616704705
1105	72-73	0.7180951703450873
1105	74-75	0.7140688474861889
1105	76-77	0.7297365928215811
1105	78-79	0.6906061240321861
1105	80-81	0.607466357160078
1105	82-83	0.7807676914771804
1105	84-85	0.6033386493701656
1105	86-87	0.6989161206395664
1105	88-89	1.2637126880780478
1105	90-91	-0.8993860038114301
1105	92-93	-0.3427973003397575
1105	94-95	-0.1164913149348048
1105	96-97	0.0
1105	98-99	0.0
1105	100-101	0.0
1105	102-103	0.0
1105	104-105	0.0
1105	106-107	0.0
1105	108-109	0.0
1105	110-111	0.0
1105	112-113	0.0
1105	114-115	0.0
1105	116-117	0.0
1105	118-119	0.0
1105	120-121	0.0
1105	122-123	0.0
1105	124-125	0.0
1106	1	-0.1257899433022729
1106	2	-0.15336467099903217
1106	3	-0.02492225203494769
1106	4	-0.07093128172146379
1106	5	-0.09236223570012925
1106	6	-0.05685860070911275
1106	7	-0.20994976982326818
1106	8	-0.10346571945622429
1106	9	-0.21965965878337101
1106	10-11	-0.32549578535994783
1106	12-13	-0.21458811135012468
1106	14-15	-0.33128251072179893
1106	16-17	-0.32703283856285736
1106	18-19	-0.3562337707325014
1106	20-21	-0.2628121991909538
1106	22-23	-0.22233926229614553
1106	24-25	-0.27003191704318397
1106	26-27	-0.35229616864417324
1106	28-29	-0.17985729570031594
1106	30-31	-0.27787344712916706
1106	32-33	-0.17635728682272855
1106	34-35	-0.37439230602235796
1106	36-37	-0.16309747595794732
1106	38-39	-0.2036977658640069
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2215	124-125	0.0
2216	1	0.01145528502864579
2216	2	-0.028383942683532837
2216	3	-0.025596760992428358
2216	4	0.009269760491228851
2216	5	-0.007335563688783964
2216	6	-0.06026198019170437
2216	7	-0.00917736070852726
2216	8	0.0775146696776261
2216	9	-0.05970082310146552
2216	10-11	-0.10062028044180948
2216	12-13	-0.07690734294951795
2216	14-15	-0.14438956878353082
2216	16-17	-0.1781918376686491
2216	18-19	-0.12962381420942393
2216	20-21	-0.12438947093712471
2216	22-23	-0.16149855433154414
2216	24-25	-0.3074074219613223
2216	26-27	-0.25933341695598244
2216	28-29	-0.20847189284502576
2216	30-31	-0.26907014164984844
2216	32-33	-0.246689299403279
2216	34-35	-0.23835733947800009
2216	36-37	-0.251379135481244
2216	38-39	-0.1448381870659432
2216	40-41	-0.14024410806265308
2216	42-43	-0.2593632220882469
2216	44-45	-0.18804948122699727
2216	46-47	-0.2012739442642939
2216	48-49	-0.11688831157526636
2216	50-51	-0.26140743390953247
2216	52-53	-0.24020064844167877
2216	54-55	-0.24553929325053048
2216	56-57	-0.4094299614677297
2216	58-59	-0.4737890424158735
2216	60-61	-0.3649289937161555
2216	62-63	-0.46111949122518325
2216	64-65	-0.5066180688072279
2216	66-67	-0.5849858337378251
2216	68-69	-0.5607396614897482
2216	70-71	-0.6067649753148885
2216	72-73	-0.6190831549935751
2216	74-75	-0.5404545193362083
2216	76-77	-0.4626432137439451
2216	78-79	-0.43123572218897266
2216	80-81	-0.43190412142619294
2216	82-83	-0.40382122119606123
2216	84-85	-0.5814319010899816
2216	86-87	-0.7066634796791789
2216	88-89	-1.7214008257733013
2216	90-91	-0.8993860038114301
2216	92-93	-0.3427973003397575
2216	94-95	-0.1164913149348048
2216	96-97	0.0
2216	98-99	0.0
2216	100-101	0.0
2216	102-103	0.0
2216	104-105	0.0
2216	106-107	0.0
2216	108-109	0.0
2216	110-111	0.0
2216	112-113	0.0
2216	114-115	0.0
2216	116-117	0.0
2216	118-119	0.0
2216	120-121	0.0
2216	122-123	0.0
2216	124-125	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5118.0
3	884.0
4	687.0
5	823.0
6	1198.0
7	1177.0
8	1053.0
9	1083.0
10	1330.0
11	1560.0
12	1812.0
13	2065.0
14	2288.0
15	2526.0
16	2695.0
17	3148.0
18	3712.0
19	4533.0
20	5117.0
21	7041.0
22	8416.0
23	11574.0
24	18336.0
25	32980.0
26	76993.0
27	198054.0
28	203351.0
29	7645.0
30	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73473600687454	19.17907216783355	14.110911189067904	23.97528063622401
2	15.612540208805934	21.104149449999507	38.40784652006572	24.875463821128843
3	19.554207997839224	23.99845847009745	29.657322514077165	26.790011017986163
4	13.274642377724888	17.200128472250817	36.689204213230994	32.8360249367933
5	14.218253033579797	37.51031507722513	33.81006422113549	14.46136766805958
6	31.88429177936289	35.91534572703672	17.277380037171234	14.922982456429157
7	28.03481387221195	32.63841674911393	21.170317667529826	18.156451711144292
8	25.441552433991433	34.24082942223261	20.454713816076474	19.862904327699482
9	25.52830726024308	17.767289644239366	21.014422372538725	35.68998072297884
10-11	24.986933199222	26.45436579304986	27.49221959468636	21.066481413041778
12-13	25.53529141105557	24.291731246795003	27.301693391796356	22.871283950353074
14-15	22.687704026115345	26.254364492089177	25.595883837427714	25.462047644367768
16-17	22.462832882548454	29.005332047101124	26.35453586167955	22.177299208670874
18-19	22.372649476966437	28.75202908046848	26.976563091643648	21.89875835092144
20-21	23.493078408486667	27.383952838683868	26.40510200889954	22.717866743929925
22-23	22.988787683737286	27.409841355025954	26.415203051409403	23.18616790982736
24-25	23.666206278942283	27.543751923998162	26.14036502875091	22.649676768308645
26-27	23.16551405115407	27.027009230252574	27.183398385734677	22.62407833285868
28-29	22.567517847133225	28.174476768065876	26.658962335202624	22.59904304959828
30-31	23.03208940850329	27.402672443736943	26.507138753397346	23.05809939436242
32-33	22.861811103017132	27.71416459858671	26.483358644162664	22.940665654233495
34-35	23.609576942641397	27.516342666747878	25.96142291084666	22.912657479764068
36-37	23.399047013088136	26.965216553888045	26.686761317851342	22.948975115172477
38-39	22.90508495223546	27.474529145176874	26.610767146424696	23.00961875616297
40-41	22.91586757295554	27.48053778115066	26.710280035360412	22.893314610533384
42-43	22.976200862938896	27.548855149071787	26.062417381255358	23.41252660673395
44-45	23.11820478271355	27.04522201928198	26.194464313199994	23.64210888480448
46-47	23.29951486437768	27.513050263474568	26.211398629372518	22.976036242775233
48-49	23.261361279298324	27.023102831800717	26.561754063655428	23.15378182524553
50-51	22.3178208881759	27.391418508424852	27.007359350474147	23.2834012529251
52-53	23.00385705043468	27.68170362114974	25.924218753858284	23.390220574557294
54-55	22.91411845854741	26.953607933453288	26.17116056715499	23.96111304084431
56-57	23.125264421137885	26.96206657568659	26.235844723676827	23.6768242794987
58-59	23.82539397720669	26.719910314934836	26.10472476331736	23.349970944541113
60-61	23.30231340715999	26.939842853591767	26.717523322561686	23.04032041668656
62-63	22.73955937766993	27.1761550985334	26.69760428275818	23.38668124103849
64-65	22.673830947983234	27.697388132183626	26.176871487411475	23.451909432421665
66-67	23.03208940850329	27.19574489800958	25.752355302991642	24.01981039049549
68-69	22.67751961465395	27.020235277074477	26.444553826297955	23.857691281973615
70-71	23.40692952116933	26.996883740301815	26.1544400527443	23.441746685784555
72-73	23.200248905687463	26.785264519909983	26.595457471203822	23.419029103198735
74-75	22.56580279492114	27.74911557817071	26.10694713552684	23.578134491381313
76-77	22.861378298782302	27.08026385319832	26.252306740043363	23.80605110797601
78-79	23.26561083998884	27.173557854950452	26.18615879872484	23.374672506335866
80-81	23.442981337012046	27.268095459940504	26.177569185739287	23.111354017308162
82-83	23.096152136622692	26.963358542375225	26.66382968916165	23.276659631840435
84-85	23.16732487295439	26.962231195850254	26.311899239290227	23.558544691905134
86-87	23.163261442524394	26.98786235552285	26.298339449073087	23.550536752879665
88-89	32.86114497254671	17.20858576267864	32.64562289046659	17.284646374308053
90-91	32.5052484644827	14.253413592380221	16.43530312878723	36.80603481434985
92-93	24.38769977679493	16.866707216046546	33.73726754058837	25.00832546657016
94-95	17.5623584623333	7.164243993442926	29.161858790633687	46.11153875359009
96-97	30.285421485295842	7.471691049326899	16.836409324106945	45.40647814127031
98-99	57.88477687494825	14.045109936249986	19.37338111627577	8.696732072525991
100-101	33.36012766311048	6.955672342655387	12.823800148216735	46.8603998460174
102-103	25.69008174148708	2.138217423613236	37.13406478122131	35.03763605367837
104-105	44.64207619955223	4.335424241039666	39.29949152431741	11.723008035090697
106-107	7.466195038954061	2.3582878123041104	9.011820542670367	81.16369660607145
108-109	21.004954051898427	10.462927476484497	21.149969066602385	47.382149405014694
110-111	42.80502163542451	2.6953372652444916	25.05478135446814	29.444859744862857
112-113	29.6196749358426	2.8706586826347307	39.386484174508126	28.12318220701454
114-115	30.71343935141877	3.948861864671032	24.83068288119738	40.507015902712816
116-117	21.9795578984085	4.599104399154019	22.11294003208049	51.30839767035699
118-119	22.8294682642994	4.595465127403769	18.02236415241486	54.55270245588197
120-121	28.025663823943166	4.465705734286752	31.187724109718275	36.32090633205181
122-123	36.19921407825047	1.7536306167777207	35.489492567913885	26.557662737057917
124-125	40.89979080732633	1.959623846653245	19.517873185958514	37.62271216006191
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	61.5
2	132.5
3	213.5
4	278.5
5	315.0
6	318.5
7	269.0
8	225.5
9	202.5
10	179.0
11	158.0
12	143.5
13	140.5
14	143.5
15	156.5
16	184.0
17	228.5
18	302.5
19	386.5
20	552.0
21	799.5
22	1109.5
23	1559.0
24	2145.5
25	2859.0
26	3730.5
27	4604.5
28	5520.5
29	6457.5
30	7386.5
31	8465.0
32	9441.0
33	10528.0
34	11764.0
35	12805.5
36	13944.5
37	15175.5
38	16541.5
39	17933.0
40	19348.5
41	20696.5
42	22069.5
43	24256.0
44	26390.0
45	26780.5
46	26015.5
47	25459.0
48	25165.5
49	24956.5
50	24284.5
51	22886.5
52	20979.0
53	19136.0
54	17510.0
55	15948.0
56	14363.5
57	12713.5
58	11184.5
59	9659.5
60	8241.0
61	6907.5
62	5576.0
63	4389.5
64	3508.5
65	2901.5
66	2262.0
67	1659.5
68	1237.5
69	912.0
70	662.5
71	504.5
72	417.5
73	324.5
74	250.5
75	177.5
76	107.0
77	62.0
78	35.5
79	22.0
80	14.5
81	10.5
82	9.5
83	11.0
84	8.0
85	5.5
86	3.5
87	5.0
88	8.0
89	8.0
90	7.0
91	5.0
92	2.0
93	4.0
94	6.0
95	6.0
96	4.5
97	1.5
98	3.5
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	8.231008183268336E-4
3	0.044447444189649014
4	0.05333693302757882
5	0.05564161531889395
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.761705728287835E-4
12-13	0.0015638915548209838
14-15	0.0
16-17	0.0
18-19	0.0067494267102800355
20-21	0.0019754419639844006
22-23	3.2924032733073344E-4
24-25	0.0
26-27	0.0
28-29	0.0013992713911556171
30-31	0.0
32-33	0.0018931318821517173
34-35	0.0
36-37	8.231008183268336E-5
38-39	0.0
40-41	0.0
42-43	0.0
44-45	8.231008183268336E-5
46-47	0.0
48-49	1.6462016366536672E-4
50-51	8.231008183268336E-5
52-53	0.0
54-55	9.877209819922003E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.6462016366536672E-4
66-67	0.0
68-69	8.231008183268336E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	7.407907364941502E-4
80-81	0.0
82-83	8.231008183268336E-4
84-85	0.0
86-87	0.0016462016366536672
88-89	19.37875642635964
90-91	26.173700611893146
92-93	40.18641587333466
94-95	44.71717432781472
96-97	48.114522955458725
98-99	93.04076489112846
100-101	51.46363787514877
102-103	52.92480644784256
104-105	74.77969706597483
106-107	81.6168663234885
108-109	12.590067807045413
110-111	39.110376173536
112-113	51.88975716879658
114-115	86.80157837812922
116-117	66.49148337583277
118-119	71.60903040369803
120-121	80.2818791062442
122-123	75.91195455166522
124-125	49.793813245009126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	607459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.629334176579555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.97878455443208	28.552609895120103
2	14.356426376752331	12.240097951920726
3	6.337652182150588	8.105096783034782
4	3.5320041179864266	6.02267935434794
5	2.130118488147416	4.54027664334733
6	1.4494773701442873	3.7074153115962316
7	1.0113469065994019	3.0179131674913124
8	0.7561901584604854	2.5788706372842145
9	0.5668612143031646	2.1748424522644156
>10	2.715854179291477	19.663052139339634
>50	0.115467409868352	3.314567343675529
>100	0.043638183958350917	3.2643565421332483
>500	0.004634143429205397	1.4868963784632336
>1k	0.0015447144764017989	1.3313253999814183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	3475	0.5720550687371494	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	2061	0.3392821573143208	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	1518	0.2498934084440267	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	1033	0.17005262906632382	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	973	0.16017541924640183	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	958	0.15770611679142132	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	920	0.15145055057213738	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	912	0.15013358926281445	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	910	0.14980434893548372	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	853	0.14042099960655782	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	744	0.12247740176703284	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	647	0.10650924589149227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.6462016366536672E-4	0.0	0.0	0.0	0.0
6	3.2924032733073344E-4	0.0	0.0	0.0	0.0
7	3.2924032733073344E-4	0.0	0.0	0.0	0.0
8	3.2924032733073344E-4	0.0	0.0	0.0	0.0
9	3.2924032733073344E-4	0.0	0.0	0.0	0.0
10-11	3.2924032733073344E-4	0.0	0.0	0.0	0.0
12-13	6.584806546614669E-4	0.0	0.0	0.0	0.0
14-15	0.0013169613093229338	0.0	0.0	0.0	0.0
16-17	0.0013992713911556171	0.0	0.0	1.6462016366536672E-4	0.0
18-19	0.001810821800319034	0.0	0.0	1.6462016366536672E-4	0.0
20-21	0.0021400621276497674	0.0	0.0	4.115504091634168E-4	0.0
22-23	0.002304682291315134	0.0	0.0	7.407907364941502E-4	0.0
24-25	0.0037862637643034346	0.0	0.0	0.0021400621276497674	0.0
26-27	0.0037862637643034346	0.0	0.0	0.005267845237291735	0.0
28-29	0.004856294828128318	0.0	0.0	0.014651194566217638	0.0
30-31	0.005267845237291735	0.0	0.0	0.03350020330590213	0.0
32-33	0.005267845237291735	0.0	0.0	0.07366752324025161	0.0
34-35	0.005432465400957102	0.0	0.0	0.14075023993388855	0.0
36-37	0.0055970855646224685	0.0	0.0	0.23680610543263003	0.0
38-39	0.006584806546614669	0.0	0.0	0.3531102510622116	0.0
40-41	0.006584806546614669	0.0	0.0	0.4744353116835869	0.0
42-43	0.007078667037610769	0.0	0.0	0.5754297820922893	0.0
44-45	0.008231008183268336	0.0	0.0	0.7064674323699213	0.0
46-47	0.009136419083427853	0.0	0.0	0.8653258903070001	0.0
48-49	0.00954796949259127	0.0	0.0	1.016364890469974	0.0
50-51	0.00954796949259127	0.0	0.0	1.1641114873596408	0.0
52-53	0.009712589656256636	0.0	0.0	1.3144096967861205	0.0
54-55	0.01053569047458347	0.0	0.0	1.4866023879800943	0.0
56-57	0.010700310638248837	0.0	0.0	1.6997854999267439	0.0
58-59	0.010700310638248837	0.0	0.0	1.9016098205804837	0.0
60-61	0.010864930801914204	0.0	0.0	2.1166860644092855	0.0
62-63	0.010864930801914204	0.0	0.0	2.333490819956573	0.0
64-65	0.01102955096557957	0.0	0.0	2.5776225226723124	0.0
66-67	0.011852651783906404	0.0	0.0	2.859781483194751	0.0
68-69	0.01201727194757177	0.0	0.0	3.191079562571301	0.0
70-71	0.012181892111237137	0.0	0.0	3.524353083911836	0.0
72-73	0.012428822356735187	0.0	0.0	3.8562273338612156	0.0
74-75	0.012593442520400554	0.0	0.0	4.210819166396416	0.0
76-77	0.012675752602233237	0.0	0.0	4.614138567376564	0.0
78-79	0.012675752602233237	0.0	0.0	4.726162588750846	0.0
80-81	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
82-83	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
84-85	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
86-87	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
88-89	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
90-91	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
92-93	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
94-95	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
96-97	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
98-99	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
100-101	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
102-103	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
104-105	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
106-107	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
108-109	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
110-111	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
112-113	0.012675752602233237	0.0	0.0	4.728384960960328	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	10	0.0013401499	2466.2014	108-109
CGCGAGT	20	0.0033936647	1548.7158	110-111
CTGCGCG	45	8.215657E-6	1376.6364	110-111
CGTATGA	25	0.0053020376	1238.9727	110-111
GTGCGAT	25	0.0053020376	1238.9727	110-111
CAGCGCG	40	1.1472099E-5	1233.1007	108-109
CGCGTTC	30	0.0076341233	1032.4772	110-111
CCGCTCC	220	0.0	1008.9007	108-109
CCGCGCT	85	9.336327E-8	870.4241	108-109
CGTGGCG	60	3.8695136E-5	822.06714	108-109
CCGTGCC	100	1.7877028E-7	739.8604	108-109
CATCGCG	75	7.554246E-5	657.6537	108-109
CCGCGCC	515	0.0	574.6489	108-109
CCACGCC	160	1.1693501E-6	462.41278	108-109
CGGCGCC	110	9.560354E-7	448.4003	108-109
CCTCGCC	310	8.185452E-11	397.7744	108-109
CTCCGCC	160	3.6827914E-4	387.17896	110-111
CGCTGCT	160	1.638835E-5	387.17896	110-111
TTTTTCG	350	0.0	375.5055	92-93
TTTCGCG	35	3.5590474E-6	352.0364	92-93
>>END_MODULE