FastQCFastQC Report
Fri 27 May 2016
SRR939919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607459
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34210.5631655798992196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.37714479495735515No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21330.3511348090982272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.3052057834355899No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15530.2556551141723145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.17482661381261946No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10230.16840642742967016No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10030.1651140241563628No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9390.15457833368177934No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9100.14980434893548372No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8820.14519498435285344No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8540.14058561977022319No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8370.13778707698791196No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8350.1374578366605812No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7520.12379436307635577No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6400.1053569047458347No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA6100.10041829983587369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG2150.038.8042871
GCGAGTT755.2987216E-627.7639626-27
GTATAGC1306.306458E-527.5041391
GAGTACT24800.026.748412-13
TGTGCGC900.00946138326.4810982
GTACTTT25400.025.88338314-15
GTATATA2558.6401997E-1025.7064821
AGTACTT26500.025.36922312-13
GTGTAGG3600.024.8301241
CGTAGAC600.001287710224.792309114-115
ACTATAC1200.001287895724.789258
ATATGGG2201.5157639E-724.374653
CGGTTAC1602.0008883E-1124.1695228-29
AGAGTAC39750.023.64800810-11
TACTTTT28400.023.46346714-15
TAGACAT2052.233137E-623.2516985
ATATAAC1300.00203459922.9163363
CAAGACT4450.022.7618454
CGCCTAG802.612378E-422.31124314-15
GTATAGA2153.3722645E-622.1738781
Produced by FastQC (version 0.11.5)