##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939919_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607459 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.82791266571077 34.0 31.0 34.0 31.0 34.0 2 32.89857093235922 34.0 31.0 34.0 31.0 34.0 3 32.921385640841606 34.0 31.0 34.0 31.0 34.0 4 36.35055369992049 37.0 37.0 37.0 35.0 37.0 5 36.236886769312825 37.0 37.0 37.0 35.0 37.0 6 36.3736795405122 37.0 37.0 37.0 35.0 37.0 7 36.36458756887296 37.0 37.0 37.0 35.0 37.0 8 36.39869192817952 37.0 37.0 37.0 35.0 37.0 9 38.23216710922054 39.0 39.0 39.0 37.0 39.0 10-11 38.22564568143694 39.0 39.0 39.0 37.0 39.0 12-13 38.209723619207224 39.0 39.0 39.0 37.0 39.0 14-15 39.75128856433109 41.0 40.0 41.0 37.0 41.0 16-17 39.75455298217658 41.0 40.0 41.0 37.0 41.0 18-19 39.782270079132914 41.0 40.0 41.0 37.0 41.0 20-21 39.78784987958035 41.0 40.0 41.0 37.0 41.0 22-23 39.72650088318718 41.0 40.0 41.0 37.0 41.0 24-25 39.67604068751965 41.0 40.0 41.0 37.0 41.0 26-27 39.56312030935421 41.0 40.0 41.0 36.5 41.0 28-29 39.47585927609929 41.0 40.0 41.0 36.0 41.0 30-31 39.34816506134571 41.0 39.0 41.0 36.0 41.0 32-33 39.17249229989184 41.0 39.0 41.0 35.0 41.0 34-35 39.03829558867348 41.0 39.0 41.0 35.0 41.0 36-37 39.01275888578489 41.0 39.0 41.0 35.0 41.0 38-39 39.05023219674085 41.0 39.0 41.0 35.0 41.0 40-41 38.98704686242199 41.0 39.0 41.0 35.0 41.0 42-43 38.84837989065929 41.0 39.0 41.0 35.0 41.0 44-45 38.65194605726477 41.0 39.0 41.0 35.0 41.0 46-47 38.39951255969538 41.0 38.5 41.0 35.0 41.0 48-49 38.12854612410055 41.0 38.0 41.0 34.0 41.0 50-51 37.91781914499579 41.0 38.0 41.0 34.0 41.0 52-53 37.78074405021573 40.5 37.5 41.0 34.0 41.0 54-55 37.620754651754275 40.0 37.0 41.0 33.0 41.0 56-57 37.420967505625896 40.0 37.0 41.0 33.0 41.0 58-59 37.21319134295483 40.0 36.0 41.0 33.0 41.0 60-61 36.95145104443263 39.5 35.5 41.0 33.0 41.0 62-63 36.69964721898927 39.0 35.0 41.0 32.0 41.0 64-65 36.420628388088744 39.0 35.0 41.0 32.0 41.0 66-67 36.08754994164215 37.5 35.0 41.0 32.0 41.0 68-69 35.71167930675156 37.0 35.0 40.0 31.5 41.0 70-71 35.31004561624735 37.0 35.0 39.0 31.0 41.0 72-73 34.924691213728 36.0 35.0 39.0 31.0 41.0 74-75 34.5675345990429 36.0 35.0 39.0 31.0 40.5 76-77 34.2072658401637 35.0 35.0 37.0 30.0 39.0 78-79 33.86704699411813 35.0 35.0 37.0 30.0 39.0 80-81 33.627828380186976 35.0 35.0 36.5 30.0 39.0 82-83 33.44920974090432 35.0 35.0 36.0 30.0 37.0 84-85 33.24892297257922 35.0 35.0 36.0 30.0 37.0 86-87 33.0955241423701 35.0 34.5 35.0 30.0 37.0 88-89 32.94418059490435 35.0 34.0 35.0 30.0 36.0 90-91 32.81841408226728 35.0 34.0 35.0 30.0 36.0 92-93 32.708293893085795 35.0 34.0 35.0 29.5 36.0 94-95 32.63262788105864 35.0 34.0 35.0 29.0 36.0 96-97 32.57799374114138 35.0 34.0 35.0 29.0 35.0 98-99 32.5187329515243 35.0 34.0 35.0 29.0 35.0 100-101 32.462398285316375 35.0 34.0 35.0 29.0 35.0 102-103 31.588473460760312 34.5 32.5 35.0 26.0 35.0 104-105 32.29232014012469 35.0 34.0 35.0 29.0 35.0 106-107 32.35189123875027 35.0 34.0 35.0 29.0 35.0 108-109 32.33469337025215 35.0 34.0 35.0 29.0 35.0 110-111 32.27771174680102 35.0 34.0 35.0 29.0 35.0 112-113 32.190093487790946 35.0 34.0 35.0 29.0 35.0 114-115 32.11035312671308 35.0 34.0 35.0 29.0 35.0 116-117 32.01654926505328 35.0 34.0 35.0 28.0 35.0 118-119 31.904275844131043 35.0 34.0 35.0 27.0 35.0 120-121 31.776533889529993 35.0 34.0 35.0 27.0 35.0 122-123 31.565257902179408 35.0 34.0 35.0 25.0 35.0 124-125 29.46394489175401 33.0 29.5 34.5 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18425515163449546 1101 2 0.23520413162380294 1101 3 0.23194247207712237 1101 4 0.09603294179615318 1101 5 0.07536196132242878 1101 6 0.014409248393043583 1101 7 0.11705470849400257 1101 8 0.17241719462749927 1101 9 0.1448157940271102 1101 10-11 0.12455788491548248 1101 12-13 0.16663669554230864 1101 14-15 0.28054955453393404 1101 16-17 0.278101632869145 1101 18-19 0.2628147485550514 1101 20-21 0.24406602588619108 1101 22-23 0.2505910091185015 1101 24-25 0.25071650785464783 1101 26-27 0.25048365501240255 1101 28-29 0.36912896449225485 1101 30-31 0.2785544639912487 1101 32-33 0.1992574918331229 1101 34-35 0.2749415842780252 1101 36-37 0.25548683958304963 1101 38-39 0.18668468884816747 1101 40-41 0.23075516236503546 1101 42-43 0.2572377425061916 1101 44-45 0.24429770242829818 1101 46-47 0.24157371197490107 1101 48-49 0.09692880420219296 1101 50-51 0.2434701912318289 1101 52-53 0.20975090826525644 1101 54-55 0.23194876448526713 1101 56-57 0.3128654744957515 1101 58-59 0.33292035533138886 1101 60-61 0.3339978828167318 1101 62-63 0.21641004167737066 1101 64-65 0.20985698894331506 1101 66-67 0.27971805117019244 1101 68-69 0.2680460244291609 1101 70-71 0.2947118829935249 1101 72-73 0.29705432881474536 1101 74-75 0.38863566534926974 1101 76-77 0.32436937742300387 1101 78-79 0.30399425636709765 1101 80-81 0.2572755745576174 1101 82-83 0.2785806083039475 1101 84-85 0.42092453381030026 1101 86-87 0.32218702193723203 1101 88-89 0.2612027807915638 1101 90-91 0.20550055449523796 1101 92-93 0.2964850731713753 1101 94-95 0.21165001462617994 1101 96-97 0.19745861347283267 1101 98-99 0.19358879790303263 1101 100-101 0.24887089246061578 1101 102-103 0.42366070927024424 1101 104-105 0.1993101019489174 1101 106-107 0.2480888731966573 1101 108-109 0.2835657160020162 1101 110-111 0.1902817560035146 1101 112-113 0.2804209491355465 1101 114-115 0.26717226815451056 1101 116-117 0.38699747859308786 1101 118-119 0.370385402373671 1101 120-121 0.26239038542562554 1101 122-123 0.39541366492409935 1101 124-125 0.46189362802308764 1102 1 0.10515605253539917 1102 2 0.13869307511274798 1102 3 0.13140193153658686 1102 4 0.03663900240221807 1102 5 -0.02824164228117354 1102 6 0.04300875699255613 1102 7 0.12151007294936989 1102 8 0.06045978267008678 1102 9 0.08139237060368743 1102 10-11 0.17789940825701223 1102 12-13 0.08701343591904731 1102 14-15 0.19753563152001163 1102 16-17 0.24835552312303122 1102 18-19 0.17844947418977597 1102 20-21 0.15683424865441253 1102 22-23 0.13881377734126232 1102 24-25 0.2000285471666885 1102 26-27 0.11511100963976162 1102 28-29 0.13025099561428988 1102 30-31 0.10636773180451797 1102 32-33 0.13890532148094792 1102 34-35 0.25899563833207395 1102 36-37 0.29387340796962036 1102 38-39 0.2862178583813346 1102 40-41 0.20205737366725174 1102 42-43 0.3118487171171722 1102 44-45 0.23669737482796904 1102 46-47 0.16712653752772155 1102 48-49 0.14030308757647703 1102 50-51 0.2579910757527202 1102 52-53 0.28721200572635297 1102 54-55 0.16284966538616885 1102 56-57 0.2383363999666841 1102 58-59 0.21020127261230925 1102 60-61 0.19484964366849056 1102 62-63 0.16452633979366738 1102 64-65 0.1333131723995038 1102 66-67 0.20789986935200488 1102 68-69 0.14374627012941232 1102 70-71 0.003319581601225252 1102 72-73 0.07334425510467923 1102 74-75 0.030273666987270076 1102 76-77 0.16024161329524134 1102 78-79 0.20943242180526767 1102 80-81 0.1302239775060201 1102 82-83 0.11915390887725152 1102 84-85 0.1312930841788571 1102 86-87 0.24376769351790273 1102 88-89 0.21279983238861178 1102 90-91 0.11585272484740727 1102 92-93 0.17733683402313005 1102 94-95 0.18201856499472768 1102 96-97 0.21360930962352853 1102 98-99 0.23172147603570892 1102 100-101 0.2920813756710956 1102 102-103 0.3107532563627906 1102 104-105 0.3461897088285255 1102 106-107 0.2714303965381859 1102 108-109 0.26965908209538725 1102 110-111 0.21626865199041134 1102 112-113 0.2499950687096657 1102 114-115 0.2845842255664621 1102 116-117 0.20090411249972107 1102 118-119 0.18900129098956597 1102 120-121 0.2235533665886038 1102 122-123 0.10663397614441195 1102 124-125 0.3213367074661697 1103 1 -0.02673482776403091 1103 2 0.021275670664458346 1103 3 0.0300976775957551 1103 4 0.042912665397970784 1103 5 -0.0562773437135462 1103 6 0.06166131583513845 1103 7 0.040388644892068726 1103 8 0.05578836540959742 1103 9 0.14718233677370307 1103 10-11 0.11129824220216022 1103 12-13 0.07499371375681108 1103 14-15 0.10686982859767369 1103 16-17 0.18739388776710086 1103 18-19 0.14104430611952523 1103 20-21 0.10845232970242336 1103 22-23 0.04182896484576304 1103 24-25 0.09422850397947258 1103 26-27 0.2969522246401226 1103 28-29 0.16619427640317497 1103 30-31 0.152122428414323 1103 32-33 0.12393519263908104 1103 34-35 0.3351498166952851 1103 36-37 0.269169600296685 1103 38-39 0.3715493202686204 1103 40-41 0.4120962421621712 1103 42-43 0.17388787352687274 1103 44-45 0.0653873625915935 1103 46-47 0.12829043301466214 1103 48-49 0.2383611024040846 1103 50-51 0.24270711980367565 1103 52-53 0.18452432128094642 1103 54-55 0.3433513568854849 1103 56-57 0.43150922081170506 1103 58-59 0.2718848542721375 1103 60-61 0.2563338443450931 1103 62-63 0.21198189294993597 1103 64-65 0.19952853265286308 1103 66-67 0.20689360720560046 1103 68-69 0.1871429447137345 1103 70-71 0.30948599634245966 1103 72-73 0.2964386191729105 1103 74-75 0.3410042507344855 1103 76-77 0.31542407928530736 1103 78-79 0.33650576809476007 1103 80-81 0.35229912326287405 1103 82-83 0.24006227999939966 1103 84-85 0.22086121201301268 1103 86-87 0.30442773814470314 1103 88-89 0.20159274968509067 1103 90-91 0.3228764058378317 1103 92-93 0.3448737231039658 1103 94-95 0.39330770340975363 1103 96-97 0.536433033801174 1103 98-99 0.48757118452199677 1103 100-101 0.4828551466348898 1103 102-103 0.40123983233630156 1103 104-105 0.44449676993843923 1103 106-107 0.5133327934287024 1103 108-109 0.5873075381523876 1103 110-111 0.5568113713454821 1103 112-113 0.435426580982039 1103 114-115 0.4779492295252936 1103 116-117 0.4392262891354335 1103 118-119 0.3751389142530712 1103 120-121 0.37595817476538684 1103 122-123 0.5096102951539869 1103 124-125 0.4463068363080289 1104 1 0.04864510977445491 1104 2 0.11208953975921077 1104 3 0.06429755443220841 1104 4 -0.02054954978633816 1104 5 -0.027399891439422674 1104 6 -0.04117474469094873 1104 7 -0.029011812572520057 1104 8 0.04633941229971583 1104 9 0.0074824379437572475 1104 10-11 0.08271632744892798 1104 12-13 0.019018907299525267 1104 14-15 0.04535128808566924 1104 16-17 0.14215602817353812 1104 18-19 0.11786866610896851 1104 20-21 0.24682162239178496 1104 22-23 0.15312564602813694 1104 24-25 0.20338081989395818 1104 26-27 0.10553188506062838 1104 28-29 0.13936084409913718 1104 30-31 0.125702748639533 1104 32-33 0.15469446289509392 1104 34-35 0.29853477974621967 1104 36-37 0.3091427682389778 1104 38-39 0.15819597285944553 1104 40-41 0.1622804376403124 1104 42-43 0.15182977772322914 1104 44-45 0.2620256576562525 1104 46-47 0.20138579678698676 1104 48-49 0.19548616838456212 1104 50-51 0.1853879612745999 1104 52-53 0.20635973299907562 1104 54-55 0.2861009280124236 1104 56-57 0.3665981844784625 1104 58-59 0.19880186183790016 1104 60-61 0.2229009904698387 1104 62-63 0.310349151241482 1104 64-65 0.4830122464735709 1104 66-67 0.21511367406581172 1104 68-69 0.15900089975904308 1104 70-71 0.2161731169547636 1104 72-73 0.2876329756433975 1104 74-75 0.24465077136437685 1104 76-77 0.13647267390129514 1104 78-79 0.1568272029500477 1104 80-81 0.24729258019962685 1104 82-83 0.2826471748705188 1104 84-85 0.16358054209131723 1104 86-87 0.19852358577379903 1104 88-89 0.23625311521689696 1104 90-91 0.17704169791137758 1104 92-93 0.1623978609591603 1104 94-95 0.1168165447927052 1104 96-97 0.17727934329356287 1104 98-99 0.31795464101887916 1104 100-101 0.340667234256955 1104 102-103 0.3841055290900641 1104 104-105 0.3234982105120281 1104 106-107 0.2571732416560266 1104 108-109 0.35104797098426843 1104 110-111 0.2527774903742497 1104 112-113 0.3484904390800452 1104 114-115 0.26219996630720743 1104 116-117 0.32209308556369365 1104 118-119 0.43024497785825133 1104 120-121 0.477717928878171 1104 122-123 0.5819496327100673 1104 124-125 0.5469133067927672 1105 1 0.05665353860687361 1105 2 0.1001158227686787 1105 3 0.022527084410619125 1105 4 0.05516461729554578 1105 5 -0.022874065164714352 1105 6 0.02198959678057122 1105 7 0.009706838814295793 1105 8 0.09715634470296663 1105 9 0.1429510062878876 1105 10-11 0.07431604095166477 1105 12-13 0.10811073174728847 1105 14-15 0.11580781524062189 1105 16-17 0.14656248059008448 1105 18-19 0.07249621967149977 1105 20-21 0.19327466175401753 1105 22-23 0.15755545419953165 1105 24-25 0.05995312931099761 1105 26-27 0.13712962629963243 1105 28-29 0.10411701001842033 1105 30-31 0.11205260746697121 1105 32-33 0.18196619931761404 1105 34-35 0.286802405555882 1105 36-37 0.3528855020041348 1105 38-39 0.2887494523479077 1105 40-41 0.4824670760724672 1105 42-43 0.3920437164377262 1105 44-45 0.3975201768391159 1105 46-47 0.48248528192234375 1105 48-49 0.4408072321613403 1105 50-51 0.38772405577717706 1105 52-53 0.4107926552711234 1105 54-55 0.22195823992075248 1105 56-57 0.4264563890956339 1105 58-59 0.3892317115306341 1105 60-61 0.3728086054839608 1105 62-63 0.4355074513577435 1105 64-65 0.36659418884195105 1105 66-67 0.41297324888726905 1105 68-69 0.45937407281999043 1105 70-71 0.5094902569647246 1105 72-73 0.38376007247116206 1105 74-75 0.3023031546894046 1105 76-77 0.26621389213747193 1105 78-79 0.4113063177150309 1105 80-81 0.2907784006613383 1105 82-83 0.4025504301437266 1105 84-85 0.1319127335877468 1105 86-87 0.11333217238732374 1105 88-89 0.1880546224416122 1105 90-91 0.321242723488524 1105 92-93 0.21676746318470208 1105 94-95 0.08499043617288038 1105 96-97 0.1806218113221263 1105 98-99 0.10612832479232992 1105 100-101 0.21112064731126168 1105 102-103 0.2033086307343055 1105 104-105 0.16595326365934682 1105 106-107 0.10872146869472488 1105 108-109 0.1317218623644436 1105 110-111 0.1590197051226383 1105 112-113 0.1378500761835113 1105 114-115 0.22813430030488036 1105 116-117 0.18004597960123192 1105 118-119 0.08363779051395426 1105 120-121 -0.04627881169648873 1105 122-123 -0.11431656061330031 1105 124-125 -0.16889905826286977 1106 1 0.0016215928947431735 1106 2 0.12650079836294736 1106 3 -0.01797055438457562 1106 4 0.0563104961002594 1106 5 -0.02244250042008389 1106 6 0.06967006029102407 1106 7 0.03125531296009143 1106 8 0.04512054963173995 1106 9 0.0348812416146842 1106 10-11 0.0015800196809792055 1106 12-13 0.020867686388342577 1106 14-15 0.006788112077799724 1106 16-17 0.058054504083081326 1106 18-19 0.05383228459471212 1106 20-21 0.042943795449801314 1106 22-23 0.0739559065984352 1106 24-25 0.14184874024794425 1106 26-27 0.019699371130769805 1106 28-29 0.09884646356815807 1106 30-31 0.07532992005874206 1106 32-33 0.17757118364238522 1106 34-35 0.03610545329587467 1106 36-37 -0.0860555274017969 1106 38-39 -0.018078199543040796 1106 40-41 0.10919333505099615 1106 42-43 0.09481465972913128 1106 44-45 -0.014031372825556332 1106 46-47 0.020113263191447572 1106 48-49 0.05188058824690245 1106 50-51 0.10516323987177856 1106 52-53 0.11873989524097084 1106 54-55 0.10608310598686188 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106-107 -0.23601337919827614 2216 108-109 -0.40609004682447747 2216 110-111 -0.35036686696217956 2216 112-113 -0.36365185347384 2216 114-115 -0.3761707105543266 2216 116-117 -0.4679302238955927 2216 118-119 -0.3729937464932789 2216 120-121 -0.3041712665018821 2216 122-123 -0.40964202427792884 2216 124-125 -0.5778164970528898 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51.0 3 1.0 4 13.0 5 35.0 6 65.0 7 174.0 8 318.0 9 535.0 10 919.0 11 1279.0 12 1546.0 13 2468.0 14 4087.0 15 4573.0 16 2064.0 17 1629.0 18 1847.0 19 1618.0 20 1545.0 21 1707.0 22 1412.0 23 1291.0 24 1585.0 25 2007.0 26 2651.0 27 3186.0 28 4303.0 29 5242.0 30 6773.0 31 8619.0 32 11252.0 33 14834.0 34 21494.0 35 37105.0 36 92633.0 37 233317.0 38 132622.0 39 659.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.07908627803517 18.970070567470472 14.433237509096717 23.517605645397637 2 15.924366912005583 20.811116470411996 38.23632541455473 25.028191203027696 3 19.40180324927279 24.060389260839003 30.2362793209089 26.301528168979303 4 13.233814957058831 17.19325913353823 36.63950982700067 32.93341608240227 5 13.884038903205278 37.01426637715137 34.077565697383974 15.024129022259372 6 31.71687307291521 35.88801877986827 16.86681734898981 15.528290798226713 7 27.580462220495537 33.04766247598604 21.154020271326953 18.217855032191473 8 25.68486103588884 33.63189943683442 20.22819647087293 20.455043056403806 9 25.818697228948785 16.7622835450623 21.07138094916694 36.347638276821975 10-11 25.12457630885377 25.900513450290475 27.43370334458787 21.541206896267898 12-13 26.02587170492165 23.652707425521722 27.761215160200116 22.560205709356516 14-15 23.03735725374058 25.39973891242043 26.518909095099424 25.043994738739567 16-17 21.97779766025169 28.409841989205848 27.22144804953754 22.390912301004924 18-19 21.905346698295688 27.10158216439299 29.172750753548797 21.820320383762525 20-21 22.90630033970685 25.777880943738058 28.939698124327602 22.376120592227487 22-23 22.459038252913373 25.808328819982318 29.14741290330015 22.585220023804155 24-25 23.034970261367434 25.987185966460284 28.81231490520348 22.1655288669688 26-27 22.664739513284022 25.554564176347704 29.89386938048494 21.886826929883334 28-29 21.933496746282465 26.477589434019418 29.439846969095857 22.149066850602264 30-31 22.50357637305563 25.716797347639925 29.23045012091351 22.54917615839094 32-33 22.470076169749728 25.97278170213957 29.296545116625154 22.26059701148555 34-35 23.037439563822414 25.752108372746147 28.849272131946353 22.361179931485086 36-37 22.887799835050597 25.40475982741222 29.26427956454674 22.44316077299044 38-39 22.38636681652589 25.88717921703358 29.161638892501387 22.564815073939144 40-41 22.54827074749078 25.806762267083045 29.243866664252238 22.40110032117394 42-43 22.74984813789902 25.986445175723794 28.414674899869784 22.8490317865074 44-45 22.801045008798948 25.51513764714985 28.438297893355763 23.245519450695436 46-47 23.242391667585796 26.00529418446348 28.071359548545665 22.680954599405062 48-49 23.331945036619757 25.748733659391004 28.120498667399772 22.798822636589467 50-51 22.350561930928674 26.29560184308735 28.36166720716954 22.992169018814437 52-53 22.936609713577376 26.676121351399846 27.279618871397084 23.10765006362569 54-55 22.763017750992248 26.006364215527306 27.513050263474568 23.717567770005875 56-57 23.044188990532692 26.09517679382477 27.394523745635507 23.466110470007028 58-59 23.58974021291972 25.811289321583843 27.581861491886695 23.017108973609744 60-61 23.093081179141308 26.149830688161668 28.090126247203518 22.666961885493507 62-63 22.419702399668125 26.356840543970865 28.175070251654844 23.048386804706162 64-65 22.312370052958308 26.798681063248715 27.72738571656688 23.161563167226102 66-67 22.663834102383866 26.198805186852113 27.36612676740323 23.771233943360787 68-69 22.376736536951462 26.049988558898622 27.82335927198379 23.749915632166122 70-71 22.99381522045109 26.08077252950405 27.425636956568262 23.499775293476596 72-73 22.83890764644198 25.73910337981658 27.796361565142668 23.62562740859877 74-75 22.32833820883385 26.80024495480353 27.280441972215407 23.59097486414721 76-77 22.607451696328475 26.019863068947863 27.322337803868248 24.050347430855414 78-79 22.870514717865735 26.227037544920726 27.28472209647071 23.617725640742833 80-81 23.04336588971437 26.28440767195811 27.190806293099616 23.481420145227908 82-83 22.774788092694322 25.96397452338347 27.71092370020034 23.550313683721864 84-85 22.716594864838616 25.981588880895668 27.29920867087326 24.00260758339246 86-87 22.667208815739002 25.86051075051979 27.351557882918847 24.12072255082236 88-89 22.498473147982004 26.00200178119017 27.034252517453854 24.465272553373975 90-91 22.70899970861943 25.814425148941567 27.15419958416536 24.32237555827364 92-93 22.784418372268746 25.80560992593739 27.179529811888536 24.230441889905325 94-95 22.680901991255332 25.465383501027233 27.363627982932098 24.490086524785333 96-97 22.14075353233716 25.7323539531063 27.57799291804056 24.54889959651598 98-99 22.538334462370948 26.04134170824077 26.733498010945766 24.68682581844252 100-101 22.21754883868706 25.54732088914643 27.01828436157831 25.216845910588205 102-103 22.593368748734477 25.20087841608542 27.1186273267079 25.0871255084722 104-105 22.8160833917061 25.156042548603047 27.111852648751782 24.91602141093907 106-107 22.40431131060517 25.429126699488254 27.516378551773013 24.650183438133567 108-109 22.075353357763007 25.66374959256442 27.224186692742542 25.036710356930026 110-111 21.81084389217185 26.285519554121095 26.943044419146585 24.96059213456047 112-113 22.018223260875473 25.609863510755904 26.699982960452612 25.671930267916014 114-115 21.77310819920962 25.663512121962924 26.76931764210062 25.794062036726835 116-117 22.137158275187403 25.56936644324691 26.756127855474883 25.537347426090808 118-119 21.966868491319563 25.30923948651594 27.208136200362826 25.515755821801672 120-121 21.820986358693997 26.102609816505378 26.642813106782132 25.43359071801849 122-123 21.86400638730441 25.775654163682905 26.53217110732482 25.828168341687864 124-125 21.78885357736968 25.919725146328798 26.517963192062567 25.773458084238964 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 244.0 1 189.0 2 164.5 3 177.5 4 111.0 5 70.5 6 169.5 7 473.0 8 806.0 9 863.0 10 595.5 11 380.5 12 380.0 13 408.0 14 485.0 15 563.0 16 641.5 17 759.5 18 822.5 19 842.0 20 919.5 21 1031.5 22 1259.0 23 1566.5 24 1807.5 25 2137.0 26 2668.0 27 3422.0 28 4253.0 29 5223.0 30 6150.5 31 7062.0 32 8093.0 33 8764.0 34 9371.5 35 10494.5 36 11675.0 37 12834.0 38 13925.0 39 14186.5 40 14468.5 41 16477.5 42 19037.0 43 20771.0 44 22038.0 45 22642.0 46 22672.0 47 25124.0 48 30882.0 49 30388.0 50 25531.5 51 22635.0 52 20783.5 53 20687.5 54 20544.5 55 19293.0 56 16959.0 57 15353.5 58 14624.0 59 13518.5 60 11582.0 61 9513.5 62 8136.0 63 6688.5 64 5240.0 65 4061.5 66 2960.0 67 2534.5 68 1808.0 69 978.5 70 798.0 71 717.0 72 619.5 73 454.5 74 376.0 75 352.5 76 205.5 77 70.0 78 31.5 79 12.5 80 5.5 81 5.0 82 3.5 83 0.5 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015968155875540572 2 0.0 3 0.0 4 0.0 5 0.15276751188146032 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.231008183268336E-5 18-19 0.0 20-21 0.003868573846136118 22-23 3.2924032733073344E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.877209819922003E-4 92-93 0.0 94-95 4.938604909961002E-4 96-97 0.0 98-99 0.0015638915548209838 100-101 0.0 102-103 3.2924032733073344E-4 104-105 0.002057752045817084 106-107 0.005514775482789785 108-109 1.6462016366536672E-4 110-111 0.004856294828128318 112-113 0.007984077937770286 114-115 0.0046916746644629515 116-117 0.0012346512274902504 118-119 1.6462016366536672E-4 120-121 4.115504091634168E-4 122-123 6.584806546614669E-4 124-125 0.0013992713911556171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 607459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.631820035657256 #Duplication Level Percentage of deduplicated Percentage of total 1 65.46925223933087 26.601348748575433 2 14.995017783244135 12.185497280005118 3 6.588431720774455 8.0309991598717 4 3.6985705388106553 6.011186100885537 5 2.257735370158186 4.586794862420272 6 1.5344463995578106 3.7408409976717114 7 1.073972023598433 3.054620659032752 8 0.7627638124797277 2.479398556671049 9 0.5737543752104557 2.0981416066399983 >10 2.851678238168265 19.835382271018847 >50 0.13484939006829744 3.7309405087424112 >100 0.051833999324020365 3.916870482538774 >500 0.004454484316907999 1.3684777065017846 >1k 0.0032396249577512715 2.359501059424743 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3421 0.5631655798992196 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2291 0.37714479495735515 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2133 0.3511348090982272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1854 0.3052057834355899 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1553 0.2556551141723145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1062 0.17482661381261946 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1023 0.16840642742967016 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1003 0.1651140241563628 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 939 0.15457833368177934 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 910 0.14980434893548372 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 882 0.14519498435285344 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 854 0.14058561977022319 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 837 0.13778707698791196 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 835 0.1374578366605812 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 752 0.12379436307635577 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 640 0.1053569047458347 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 610 0.10041829983587369 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 1.6462016366536672E-4 0.0 0.0 0.0 0.0 4 1.6462016366536672E-4 0.0 0.0 0.0 0.0 5 3.2924032733073344E-4 0.0 0.0 0.0 0.0 6 6.584806546614669E-4 0.0 0.0 0.0 0.0 7 0.0013169613093229338 0.0 0.0 0.0 0.0 8 0.0013169613093229338 0.0 0.0 0.0 0.0 9 0.0013169613093229338 0.0 0.0 1.6462016366536672E-4 0.0 10-11 0.0014815814729883005 0.0 0.0 1.6462016366536672E-4 0.0 12-13 0.0015638915548209838 0.0 0.0 1.6462016366536672E-4 0.0 14-15 0.0019754419639844006 0.0 0.0 1.6462016366536672E-4 0.0 16-17 0.0019754419639844006 0.0 0.0 3.2924032733073344E-4 0.0 18-19 0.002057752045817084 0.0 0.0 3.2924032733073344E-4 0.0 20-21 0.002304682291315134 0.0 0.0 5.761705728287835E-4 0.0 22-23 0.002304682291315134 0.0 0.0 8.231008183268336E-4 0.0 24-25 0.0032924032733073344 0.0 0.0 0.002057752045817084 0.0 26-27 0.003621643600638068 0.0 0.0 0.005103225073626368 0.0 28-29 0.0044447444189649014 0.0 0.0 0.014404264320719588 0.0 30-31 0.0044447444189649014 0.0 0.0 0.03317096297857139 0.0 32-33 0.0044447444189649014 0.0 0.0 0.07342059299475356 0.0 34-35 0.004773984746295635 0.0 0.0 0.14017406936105975 0.0 36-37 0.0055970855646224685 0.0 0.0 0.23598300461430322 0.0 38-39 0.006173256137451252 0.0 0.0 0.35154635950739066 0.0 40-41 0.006255566219283935 0.0 0.0 0.47262448988326783 0.0 42-43 0.006667116628447352 0.0 0.0 0.5735366502101377 0.0 44-45 0.007490217446774186 0.0 0.0 0.7040804399967735 0.0 46-47 0.008724868674264436 0.0 0.0 0.8626096576065216 0.0 48-49 0.009218729165260536 0.0 0.0 1.0124140065420053 0.0 50-51 0.009218729165260536 0.0 0.0 1.1600782933498393 0.0 52-53 0.00930103924709322 0.0 0.0 1.3102941926944864 0.0 54-55 0.00954796949259127 0.0 0.0 1.4825691939702925 0.0 56-57 0.010206450147252737 0.0 0.0 1.6962461664079387 0.0 58-59 0.010206450147252737 0.0 0.0 1.8989758979618379 0.0 60-61 0.010206450147252737 0.0 0.0 2.114957552690799 0.0 62-63 0.010206450147252737 0.0 0.0 2.3329146493837447 0.0 64-65 0.010206450147252737 0.0 0.0 2.577210972263148 0.0 66-67 0.010700310638248837 0.0 0.0 2.8594522428674196 0.0 68-69 0.010864930801914204 0.0 0.0 3.1906680121621376 0.0 70-71 0.011358791292910304 0.0 0.0 3.525587735139326 0.0 72-73 0.011688031620241037 0.0 0.0 3.8597666673800206 0.0 74-75 0.01177034170207372 0.0 0.0 4.214523120078885 0.0 76-77 0.011852651783906404 0.0 0.0 4.6180071412227 0.0 78-79 0.011934961865739087 0.0 0.0 5.080919041449711 0.0 80-81 0.013087303011396654 0.0 0.0 5.54621793404987 0.0 82-83 0.013663473584225438 0.0 0.0 6.030283525307881 0.0 84-85 0.014075023993388855 0.0 0.0 6.5191231013121875 0.0 86-87 0.014404264320719588 0.0 0.0 7.0473892065143495 0.0 88-89 0.014486574402552271 0.0 0.0 7.631955407690066 0.0 90-91 0.015309675220879105 0.0 0.0 8.266072278129059 0.0 92-93 0.015391985302711788 0.0 0.0 8.922083630335546 0.0 94-95 0.015474295384544472 0.0 0.0 9.559822144375175 0.0 96-97 0.01563891554820984 0.0 0.0 10.22834462902023 0.0 98-99 0.01621508612103862 0.0 0.0 10.939915286463778 0.0 100-101 0.01662663653020204 0.0 0.0 11.732232134185189 0.0 102-103 0.01720280710303082 0.0 0.0 12.592043249009398 0.0 104-105 0.01860207849418644 0.0 0.0 13.390697973031925 0.0 106-107 0.01917824906701522 0.0 0.0 14.212070279640272 0.0 108-109 0.01934286923068059 0.0 0.0 15.079618542156755 0.0 110-111 0.019672109558011325 0.0 0.0 15.992601969844879 0.0 112-113 0.019836729721676688 0.0 0.0 16.952584454259465 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATATG 215 0.0 38.804287 1 GCGAGTT 75 5.2987216E-6 27.76396 26-27 GTATAGC 130 6.306458E-5 27.504139 1 GAGTACT 2480 0.0 26.7484 12-13 TGTGCGC 90 0.009461383 26.481098 2 GTACTTT 2540 0.0 25.883383 14-15 GTATATA 255 8.6401997E-10 25.706482 1 AGTACTT 2650 0.0 25.369223 12-13 GTGTAGG 360 0.0 24.830124 1 CGTAGAC 60 0.0012877102 24.792309 114-115 ACTATAC 120 0.0012878957 24.78925 8 ATATGGG 220 1.5157639E-7 24.37465 3 CGGTTAC 160 2.0008883E-11 24.16952 28-29 AGAGTAC 3975 0.0 23.648008 10-11 TACTTTT 2840 0.0 23.463467 14-15 TAGACAT 205 2.233137E-6 23.251698 5 ATATAAC 130 0.002034599 22.916336 3 CAAGACT 445 0.0 22.761845 4 CGCCTAG 80 2.612378E-4 22.311243 14-15 GTATAGA 215 3.3722645E-6 22.173878 1 >>END_MODULE