FastQCFastQC Report
Fri 27 May 2016
SRR939918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675929
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA73370.43778704229117105No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC59610.3556833254869389No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA40850.24374540926256424No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA33090.19744273176250307No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA27940.1667135063597563No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA22530.13443290258716212No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA21750.12977876747761988No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA17940.10704510751947129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCT400.03047.7876110-111
TGTCGCG108.7983836E-43047.7876110-111
CGACGCC257.366907E-102934.5989108-109
CCGCTCC3150.02639.5864108-109
TAGCGCG100.00136708792445.499108-109
ACGCTCG100.00136708792445.499108-109
CTCCGCC1250.02438.2302110-111
CCGCGCC2200.02112.022108-109
CCGTTCG700.02096.142108-109
CTCGTCG150.0019794212031.8584110-111
CGCGGAC150.00307553031630.3328108-109
CGTGTCG150.00307553031630.3328108-109
CCGCGAC150.00307553031630.3328108-109
CGCGGCG200.0035185871523.8938110-111
CGTTGCG200.0035185871523.8938110-111
CGCTGTT801.6370905E-111523.8938110-111
CGGTGCG601.0108124E-81523.8938110-111
CGTCGAT200.0035185871523.8938110-111
CAGCGCG357.955799E-61397.4281108-109
CCGCTCG1100.01333.9086108-109
Produced by FastQC (version 0.11.5)