##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1675929 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.166389506954054 34.0 31.0 34.0 30.0 34.0 2 32.30526710857083 34.0 31.0 34.0 30.0 34.0 3 32.47981805911826 34.0 31.0 34.0 31.0 34.0 4 35.67571299261484 37.0 37.0 37.0 35.0 37.0 5 35.761328791374815 37.0 37.0 37.0 35.0 37.0 6 35.80664395687407 37.0 37.0 37.0 35.0 37.0 7 35.74981577381858 37.0 37.0 37.0 35.0 37.0 8 35.824500918595 37.0 37.0 37.0 35.0 37.0 9 37.534369296074 39.0 39.0 39.0 35.0 39.0 10-11 37.50501095213461 39.0 39.0 39.0 35.0 39.0 12-13 37.46975975712575 39.0 39.0 39.0 35.0 39.0 14-15 39.074370393972536 41.0 40.0 41.0 36.0 41.0 16-17 39.086227399848084 41.0 40.0 41.0 36.0 41.0 18-19 39.08493170056727 41.0 40.0 41.0 36.0 41.0 20-21 39.12642391175282 41.0 40.0 41.0 36.0 41.0 22-23 39.133086485167325 41.0 40.0 41.0 36.0 41.0 24-25 39.16209755902547 41.0 40.0 41.0 36.0 41.0 26-27 39.12119218654251 41.0 40.0 41.0 36.0 41.0 28-29 39.07001281080523 41.0 40.0 41.0 36.0 41.0 30-31 38.966129830082295 41.0 39.0 41.0 36.0 41.0 32-33 38.88351773851995 41.0 39.0 41.0 35.0 41.0 34-35 38.87978577851449 41.0 39.0 41.0 35.0 41.0 36-37 39.027361839314196 41.0 40.0 41.0 36.0 41.0 38-39 38.958984837663166 41.0 40.0 41.0 35.0 41.0 40-41 38.838928439092584 41.0 39.0 41.0 35.0 41.0 42-43 38.84006512209049 41.0 39.0 41.0 35.0 41.0 44-45 38.80047722785392 41.0 39.0 41.0 35.0 41.0 46-47 38.69438383129595 41.0 39.0 41.0 35.0 41.0 48-49 38.628752172675576 41.0 39.0 41.0 34.0 41.0 50-51 38.540863306261784 41.0 39.0 41.0 34.0 41.0 52-53 38.47480472024769 41.0 39.0 41.0 34.0 41.0 54-55 38.36312636155827 41.0 39.0 41.0 33.5 41.0 56-57 38.17261530768905 40.5 38.0 41.0 33.0 41.0 58-59 38.0394372315295 40.0 38.0 41.0 33.0 41.0 60-61 37.86348854873924 40.0 38.0 41.0 33.0 41.0 62-63 37.62150574397842 40.0 37.5 41.0 32.0 41.0 64-65 37.3456420289881 40.0 37.0 41.0 32.0 41.0 66-67 37.03602867424575 39.5 36.5 41.0 31.0 41.0 68-69 36.59368326462517 39.0 36.0 41.0 31.0 41.0 70-71 36.13414052743285 39.0 35.0 40.5 30.5 41.0 72-73 35.7458221678842 38.0 35.0 40.0 30.0 41.0 74-75 35.303308195036905 37.0 35.0 39.5 30.0 41.0 76-77 34.782194233765274 37.0 35.0 39.0 29.0 41.0 78-79 34.27438572875104 36.0 34.5 39.0 29.0 40.0 80-81 33.80803273885708 36.0 34.0 37.5 28.5 39.0 82-83 33.56046049683489 35.5 34.0 37.0 29.0 39.0 84-85 33.06528737195907 35.0 34.0 37.0 28.5 39.0 86-87 32.404134662029236 35.0 34.0 36.5 27.0 37.5 88-89 16.56694645178883 18.0 17.0 18.5 2.0 19.5 90-91 2.8223361490850745 2.0 2.0 2.0 2.0 2.0 92-93 2.3244388634602062 2.0 2.0 2.0 2.0 2.0 94-95 2.1109011777945246 2.0 2.0 2.0 2.0 2.0 96-97 2.0000092486018204 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14251249173574365 1101 2 0.14401302507172176 1101 3 0.21307112180524257 1101 4 0.18535010914798278 1101 5 0.16947091147380178 1101 6 0.20493601277966178 1101 7 0.20579503611590866 1101 8 0.2110140008200574 1101 9 0.22666528335947334 1101 10-11 0.15780449831208188 1101 12-13 0.2539092817502038 1101 14-15 0.2818588794685155 1101 16-17 0.29266370989645196 1101 18-19 0.22443308434426257 1101 20-21 0.26039863442510125 1101 22-23 0.2859512465734255 1101 24-25 0.2776366136121027 1101 26-27 0.19893961122792092 1101 28-29 0.23260639700527008 1101 30-31 0.23291994863955523 1101 32-33 0.29297526780563743 1101 34-35 0.21251816587127337 1101 36-37 0.2902340978134603 1101 38-39 0.20873861581937803 1101 40-41 0.22454051600927727 1101 42-43 0.228537120693737 1101 44-45 0.20800979044334866 1101 46-47 0.18909868041244238 1101 48-49 0.17136523450604813 1101 50-51 0.10440307831621709 1101 52-53 0.15272948832709687 1101 54-55 0.22243835961085523 1101 56-57 0.14640161701539967 1101 58-59 0.05192322645378766 1101 60-61 0.08519610309663506 1101 62-63 0.14411247813252004 1101 64-65 0.07158732812342805 1101 66-67 0.13386905146211348 1101 68-69 0.07815271515576683 1101 70-71 0.10016314811416294 1101 72-73 0.05670508210432956 1101 74-75 0.10116796077165446 1101 76-77 0.07050371130375765 1101 78-79 0.08039809074977455 1101 80-81 0.05518467948590455 1101 82-83 0.1789199454332291 1101 84-85 0.22634066319319857 1101 86-87 0.26179505682227955 1101 88-89 0.5848551985985821 1101 90-91 -0.084076390003613 1101 92-93 -0.22730775396625802 1101 94-95 -0.04665122941863009 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.12194691982043793 1102 2 -0.031229796622710637 1102 3 0.05572266381162194 1102 4 0.007503010010566413 1102 5 0.019566622214675533 1102 6 -0.0070266482152590015 1102 7 -0.07416715223337178 1102 8 0.05787681542481238 1102 9 0.10212788527368843 1102 10-11 0.10250495914157653 1102 12-13 0.014552079812361285 1102 14-15 0.12032987084273827 1102 16-17 0.14389140693059943 1102 18-19 0.06950220876822044 1102 20-21 0.023685884809125923 1102 22-23 0.10514538576756394 1102 24-25 0.04933695628018597 1102 26-27 0.1129630071867922 1102 28-29 0.02072881222446199 1102 30-31 0.06268953389300691 1102 32-33 0.06596120659803262 1102 34-35 -0.03729159426106321 1102 36-37 0.08421022920894927 1102 38-39 0.09260013064863415 1102 40-41 0.0912639001520077 1102 42-43 0.0624636242976635 1102 44-45 0.08526371931018417 1102 46-47 0.05155880093708021 1102 48-49 0.092766628810665 1102 50-51 0.08816770079051395 1102 52-53 0.03382129974148995 1102 54-55 0.051910178114937366 1102 56-57 0.04449449188246035 1102 58-59 -0.035199543249625265 1102 60-61 -0.07553177117962662 1102 62-63 -0.05921494713038555 1102 64-65 -0.07437509654867824 1102 66-67 -0.010030275017861356 1102 68-69 0.06341562428964664 1102 70-71 0.17228414539644632 1102 72-73 0.23514062505600464 1102 74-75 0.24148227011822598 1102 76-77 0.17968485274295887 1102 78-79 0.07136110622892744 1102 80-81 0.09730685838227515 1102 82-83 0.08933469197700816 1102 84-85 0.13352015037268927 1102 86-87 0.32848062931431343 1102 88-89 0.8256256109818985 1102 90-91 0.11406847588318847 1102 92-93 -0.028288493656675406 1102 94-95 0.09758131223326671 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.03326096557617575 1103 2 0.057013870380259846 1103 3 0.02228498486525865 1103 4 0.061867437973006645 1103 5 -0.03495850526330457 1103 6 0.004817669584390671 1103 7 -0.012801751711641884 1103 8 0.021360577320479024 1103 9 -0.026572248339597593 1103 10-11 -0.056100827131700726 1103 12-13 -0.007477024251478781 1103 14-15 -0.02366098528212035 1103 16-17 0.014219077605666541 1103 18-19 -0.006801066120665666 1103 20-21 0.00547893873617511 1103 22-23 0.06917187210174092 1103 24-25 -0.014239971341410751 1103 26-27 0.11891425197178762 1103 28-29 0.06019726767306821 1103 30-31 0.024035755909210366 1103 32-33 0.04955176822829088 1103 34-35 0.05141926477237746 1103 36-37 0.04550037000281293 1103 38-39 0.04182399198167985 1103 40-41 0.12414322099659358 1103 42-43 0.11547710378756904 1103 44-45 0.13065560616609417 1103 46-47 -0.007646881717732867 1103 48-49 0.016994989366573066 1103 50-51 0.1261275077329529 1103 52-53 0.12670243845388995 1103 54-55 0.08589567152971256 1103 56-57 0.06828665505427978 1103 58-59 0.06835602442505007 1103 60-61 0.08113016903070047 1103 62-63 0.007882554210283388 1103 64-65 0.07995588264583375 1103 66-67 0.06779973616202994 1103 68-69 -0.01385066607837615 1103 70-71 0.08442350314374636 1103 72-73 0.10286738134355033 1103 74-75 0.20551284665500447 1103 76-77 0.052177422208231405 1103 78-79 0.08817492929585313 1103 80-81 0.04904773950280372 1103 82-83 0.08361140781700271 1103 84-85 0.16138715516277102 1103 86-87 0.16974095707588077 1103 88-89 0.7668332205766042 1103 90-91 -0.5633663308320154 1103 92-93 -0.34291214957065375 1103 94-95 -0.11983804260544328 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.15877430061452458 1104 2 2.5067036130366205E-4 1104 3 0.03903888858092586 1104 4 0.005072530172959944 1104 5 -0.006338225802771547 1104 6 -0.005474467094956026 1104 7 0.04062829848421501 1104 8 0.12231954605800155 1104 9 0.11190050979832478 1104 10-11 0.029038746339409727 1104 12-13 0.1171629530582905 1104 14-15 0.15942444663012623 1104 16-17 0.19411683220695863 1104 18-19 0.13850680946497818 1104 20-21 0.10295074842817087 1104 22-23 0.02335918076618526 1104 24-25 0.10107600192370114 1104 26-27 0.13442406082613445 1104 28-29 0.14742154064152402 1104 30-31 0.10305067529129985 1104 32-33 0.08270765413324455 1104 34-35 0.10760092317991621 1104 36-37 0.12191316071263714 1104 38-39 0.14577638431633488 1104 40-41 0.2034433361899204 1104 42-43 0.11273124478893237 1104 44-45 0.08015260228938104 1104 46-47 0.005983979779195181 1104 48-49 0.0577914052026145 1104 50-51 0.02695184035384557 1104 52-53 0.03400160450441092 1104 54-55 0.03343026959699813 1104 56-57 0.07173513757562944 1104 58-59 0.012025234915242322 1104 60-61 -0.011898195844324277 1104 62-63 -0.05209672074774119 1104 64-65 -0.08630020890672796 1104 66-67 -0.11588326547074246 1104 68-69 -0.18462407112472334 1104 70-71 -0.22000272887776617 1104 72-73 -0.19154093403946604 1104 74-75 -0.2128234147118917 1104 76-77 -0.11883866931645315 1104 78-79 0.040038162137584266 1104 80-81 -0.012457167537647251 1104 82-83 -0.022499204254671668 1104 84-85 -0.03901351272156717 1104 86-87 0.01651589245820162 1104 88-89 0.7182886077286099 1104 90-91 -0.6637036876853211 1104 92-93 -0.34291214957065375 1104 94-95 -0.11983804260544328 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.12052915797256958 1105 2 -0.17473703145611097 1105 3 -0.07128649588570823 1105 4 -0.005527655063112036 1105 5 0.0780161111301112 1105 6 -0.06071043953345878 1105 7 -0.05615042582954999 1105 8 -0.15291038754877206 1105 9 -7.023284525828899E-4 1105 10-11 -0.07452942291072873 1105 12-13 -0.03978085527326414 1105 14-15 -0.06627705866742417 1105 16-17 0.07038948206666618 1105 18-19 0.008295518206693941 1105 20-21 -0.02069484000170263 1105 22-23 -0.1318504990060987 1105 24-25 -0.07076658812443526 1105 26-27 -0.06148276475001069 1105 28-29 -0.06550376443822614 1105 30-31 -0.11888487205415288 1105 32-33 -0.0625201421342112 1105 34-35 0.04957419626063597 1105 36-37 -0.08246838488901886 1105 38-39 -0.05812069770659889 1105 40-41 -0.09047644234102137 1105 42-43 -0.0394771695427778 1105 44-45 -0.12338622095266061 1105 46-47 -0.051773204903881265 1105 48-49 -0.07194729658426269 1105 50-51 -0.10095349926258734 1105 52-53 -0.07962885292013766 1105 54-55 -0.13473524867385578 1105 56-57 -0.13343196504041543 1105 58-59 -0.1796559351253677 1105 60-61 -0.27733317832153404 1105 62-63 -0.14423419576971241 1105 64-65 -0.1991222092527778 1105 66-67 -0.26312864220224697 1105 68-69 -0.13020842987204162 1105 70-71 -0.13431747414423967 1105 72-73 -0.17048661508736984 1105 74-75 -0.211994432390739 1105 76-77 -0.19611230642337318 1105 78-79 -0.3529081092230868 1105 80-81 -0.4434591047134404 1105 82-83 -0.33642396991068324 1105 84-85 -0.2689489854297804 1105 86-87 -0.21814886756608587 1105 88-89 0.31100859252975255 1105 90-91 -0.8203401262673493 1105 92-93 -0.34291214957065375 1105 94-95 -0.11983804260544328 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.11534069649138701 1106 2 -0.06070902408940526 1106 3 -0.05058030517550094 1106 4 -0.07336187448559173 1106 5 -0.09803069026181532 1106 6 -0.11450424137711934 1106 7 -0.17182750218213982 1106 8 -0.0574485539151155 1106 9 0.03790385580431632 1106 10-11 -0.11836874541242537 1106 12-13 -0.13502650301187913 1106 14-15 -0.18378364849004925 1106 16-17 -0.1254051934559257 1106 18-19 -0.19057639351202482 1106 20-21 -0.04466541290673831 1106 22-23 -0.08583169505270405 1106 24-25 -0.18940722313016778 1106 26-27 -0.2280878767645902 1106 28-29 -0.05798838818541441 1106 30-31 -0.04155187627858936 1106 32-33 -0.03612188353518064 1106 34-35 -0.08824766259057526 1106 36-37 -0.03625697444359588 1106 38-39 -0.037040711407470894 1106 40-41 -0.02109715151018321 1106 42-43 -0.2939689217158019 1106 44-45 -0.04887431459516023 1106 46-47 -0.1783593541143489 1106 48-49 -0.05164443776779848 1106 50-51 -0.03787611414391989 1106 52-53 -0.11692749722855922 1106 54-55 -0.05303683668460479 1106 56-57 -0.2115800635477143 1106 58-59 -0.11336678711125359 1106 60-61 -0.09653103185124223 1106 62-63 0.009821461549584853 1106 64-65 -0.05540003915344727 1106 66-67 -0.1124838075807304 1106 68-69 -0.027604519165997488 1106 70-71 -0.027489127234133548 1106 72-73 0.00843712167714017 1106 74-75 -0.13920258143242137 1106 76-77 -0.10127445825487769 1106 78-79 -0.08771875420662667 1106 80-81 -0.06989757239972505 1106 82-83 -0.006673713019296201 1106 84-85 -0.056186323290361884 1106 86-87 0.08086476877498683 1106 88-89 0.6639443523077588 1106 90-91 -0.251783632499758 1106 92-93 -0.3258232874878231 1106 94-95 -0.11983804260544328 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.029373860254231943 1107 2 0.001319863311202596 1107 3 0.07874327348775267 1107 4 0.15683106342686415 1107 5 0.17873812094826746 1107 6 0.1328731533592844 1107 7 0.03153095822489149 1107 8 -0.00802374899489422 1107 9 0.07743793040155111 1107 10-11 0.15696181767665962 1107 12-13 0.15692015688543393 1107 14-15 0.10629206286543535 1107 16-17 0.12854596235901994 1107 18-19 0.10165793203542961 1107 20-21 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0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9945.0 3 1623.0 4 1196.0 5 1182.0 6 1237.0 7 1404.0 8 1558.0 9 1936.0 10 2282.0 11 2864.0 12 3629.0 13 4576.0 14 5362.0 15 6216.0 16 7052.0 17 7861.0 18 10091.0 19 12652.0 20 13713.0 21 20209.0 22 23814.0 23 29753.0 24 46139.0 25 79476.0 26 140240.0 27 408414.0 28 799988.0 29 30233.0 30 1284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.82370374878649 20.82331650087802 14.575140116317575 24.77783963401791 2 11.892056706865555 19.794118682488453 47.8628118218265 20.451012788819494 3 18.980622515133064 25.478109292357654 29.49246033167377 26.04880786083551 4 11.062773726113889 17.30796784777843 40.71456545307791 30.914692973029766 5 11.154460847029297 39.81022168862578 36.458056593196325 12.5772608711486 6 29.31096723071204 38.02374682937046 20.024416308805446 12.640869631112059 7 27.736855200906486 31.701760635444582 22.83509623617707 17.72628792747187 8 22.388418602458696 42.51976068198593 20.807385038387665 14.284435677167709 9 25.532764216145193 17.732195098957057 22.702393717156273 34.03264696774148 10-11 23.48749628189626 26.24335328355177 32.08467699710279 18.18447343744919 12-13 26.942977819983945 24.442501210543227 29.86134916720891 18.753171802263918 14-15 19.007428118971628 32.27917173102202 25.644045779982328 23.06935437002403 16-17 18.466078773120785 31.246975554424505 32.27324340499318 18.013702267461532 18-19 20.57899226381267 28.11811253617993 32.52737233083039 18.77552286917701 20-21 21.199312128332696 29.212587430947302 29.14733867416436 20.44076176655564 22-23 19.49340751335234 32.98516697962854 29.535605156084138 17.98582035093498 24-25 20.771613833282913 29.414014555509215 32.0788947503146 17.73547686089327 26-27 17.75206467577087 32.21631107284378 31.04376736723334 18.987856884152013 28-29 18.424706577322173 31.584721136576547 28.18594077247585 21.804631513625434 30-31 20.03763285914857 29.314338495246517 30.48941810780767 20.158610537797244 32-33 18.159100523954464 31.64563231058488 31.714072780801345 18.48119438465931 34-35 18.45389539407421 31.811420299678744 31.704762973111016 18.029921333136027 36-37 17.70537415367835 30.48807556883376 31.188851079013492 20.617699198474398 38-39 20.116723321811367 31.76835056855034 29.108840529640574 19.00608557999772 40-41 22.77584551612867 28.13973622987609 31.29974479825816 17.78467345573709 42-43 18.402181715335196 32.21854863780029 31.636214899318528 17.74305474754599 44-45 17.6477397454605 32.28789295008707 30.272144664882777 19.792222639569648 46-47 20.68491564976798 28.859516125086444 30.334101265626405 20.121466959519168 48-49 19.613098052810727 30.138845422104822 29.586077210334448 20.661979314750003 50-51 17.823010401991972 32.69711903069879 29.83047014521498 19.649400422094253 52-53 17.876234613757504 30.986097859754203 31.475915745834097 19.661751780654193 54-55 18.307500540006423 29.715867119038897 31.96787289979271 20.008759441161974 56-57 19.185806797304657 30.114193381700538 28.541274719871783 22.158725101123018 58-59 19.84937906080747 31.18876157641523 27.573900803673663 21.387958559103637 60-61 18.715589980243795 28.381721421372863 32.270460144791336 20.63222845359201 62-63 19.844909851464426 28.64892505855709 31.34584798814508 20.160317101833403 64-65 20.495373605174926 30.108948659458317 28.832878759039026 20.562798976327727 66-67 20.191875670150704 32.31255620017316 27.704335923538526 19.79123220613761 68-69 21.49959842999853 28.624482523649668 31.27039921905673 18.605519827295065 70-71 22.622259057513773 28.896421029769158 29.613247339236924 18.86807257348014 72-73 20.71830011891912 31.180736176771212 28.683912027299485 19.417051677010182 74-75 19.157523976254364 31.75743125156257 31.134433499271154 17.95061127291192 76-77 20.17958397998961 29.021336822741294 31.856719467232804 18.942359730036294 78-79 19.407640556707488 29.22726255649865 30.386383638885988 20.97871324790787 80-81 20.297667741294532 30.675076330799218 30.03710181039889 18.990154117507366 82-83 19.363722612057856 30.52245930593346 31.719682323738603 18.39413575827008 84-85 19.138835062972593 29.738816267410133 31.716019935229895 19.406328734387376 86-87 17.64856108024894 32.73570776473677 28.693277085285008 20.922454069729284 88-89 28.090899712360933 18.773006225708848 36.71574538230038 16.420348679629836 90-91 31.100750658837974 16.721910132802652 18.358027796129374 33.81931141223001 92-93 22.82349132633792 18.59516848745961 37.636509512952045 20.94483067325043 94-95 15.901791573023383 7.421790967652663 29.40134240957005 47.27507504975391 96-97 27.479931812871904 7.365882783903915 16.46269177629776 48.691493626926416 98-99 57.145427980136 13.954933222501682 19.126172408917377 9.773466388444943 100-101 30.58262474344159 7.187331373674705 13.647059403281483 48.58298447960222 102-103 22.696245733788395 2.0849896771353955 38.497161371242775 36.72160321783343 104-105 39.3241793733666 4.285451840965255 44.96696159248088 11.423407193187272 106-107 5.994355499486272 2.0479197284396986 7.351506717475842 84.60621805459819 108-109 18.888723484455323 12.69707407144379 24.133618405598376 44.28058403850251 110-111 40.64300484350345 3.035138057239267 27.173048820574824 29.14880827868246 112-113 26.756458746718785 2.6986050646805952 40.728535206160856 29.816400982439756 114-115 28.120313318757567 3.8139421426377322 25.728411903944494 42.33733263466021 116-117 19.861745046412512 4.365594897335827 21.791961329872514 53.98069872637915 118-119 20.499748909739658 4.284392758028281 17.40314523589269 57.812713096339365 120-121 25.430560142954338 4.237031444779473 32.013887514783264 38.31852089748293 122-123 34.39277916927315 1.7072947599279007 35.82178859215559 28.078137478643363 124-125 38.98564658284309 2.200011436985782 21.275891072241244 37.538450907929885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 23.5 2 74.0 3 122.5 4 152.0 5 166.5 6 150.0 7 123.0 8 102.0 9 100.0 10 96.5 11 84.0 12 76.0 13 84.0 14 114.5 15 164.0 16 226.5 17 311.5 18 480.0 19 749.0 20 1177.5 21 1753.0 22 2522.5 23 3712.0 24 5421.5 25 7712.0 26 10709.0 27 14641.0 28 19938.5 29 27039.5 30 36317.5 31 48409.5 32 63413.5 33 80541.0 34 97047.5 35 108280.0 36 112026.0 37 108892.5 38 102035.0 39 94628.0 40 86824.0 41 78335.5 42 69699.5 43 61776.0 44 55101.5 45 49551.5 46 44931.0 47 40884.0 48 37279.5 49 33945.5 50 30205.0 51 26418.0 52 22774.0 53 19151.5 54 15800.0 55 12742.0 56 10220.0 57 8164.5 58 6214.5 59 4513.0 60 3277.5 61 2411.5 62 1755.0 63 1236.5 64 864.0 65 620.0 66 433.0 67 317.5 68 238.0 69 162.5 70 123.5 71 90.0 72 50.0 73 38.5 74 35.0 75 28.5 76 21.5 77 12.5 78 7.0 79 5.0 80 5.5 81 4.5 82 3.0 83 2.0 84 3.0 85 3.5 86 1.5 87 0.5 88 0.5 89 1.5 90 2.5 91 3.0 92 2.5 93 2.5 94 2.0 95 1.5 96 2.5 97 3.5 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 7.1602078608342E-4 3 0.047078366684984864 4 0.05352255375973564 5 0.05501426373074277 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.475129913021375E-4 12-13 0.0017005493669481225 14-15 0.0 16-17 1.1933679768057E-4 18-19 0.006175679279969497 20-21 0.002088393959409975 22-23 3.5801039304171E-4 24-25 0.0 26-27 0.0 28-29 0.0011336995779654148 30-31 0.0 32-33 0.0011933679768057 34-35 5.9668398840285E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.98341994201425E-5 46-47 0.0 48-49 2.98341994201425E-5 50-51 0.0 52-53 0.0 54-55 0.001372373173326555 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.98341994201425E-5 64-65 1.1933679768057E-4 66-67 0.0 68-69 4.17678791881995E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.3867359536114E-4 80-81 0.0 82-83 5.370155895625651E-4 84-85 8.95025982604275E-5 86-87 0.0011336995779654148 88-89 19.315466227984597 90-91 26.211253579358072 92-93 40.28112169429612 94-95 44.80356864759784 96-97 48.28345950216268 98-99 93.0370856999312 100-101 51.56680265094762 102-103 53.093537972074 104-105 74.82843843623446 106-107 81.6486259262773 108-109 12.620164696714479 110-111 39.099717231457895 112-113 51.9465323411672 114-115 86.79108721192843 116-117 66.44878750830136 118-119 71.78057662347271 120-121 80.39913982036232 122-123 75.86765310463629 124-125 49.915420044643895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1675929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.54647380893748 #Duplication Level Percentage of deduplicated Percentage of total 1 81.60486118049846 54.3051575723002 2 11.943728457447717 15.896260259492115 3 3.250736417548577 6.489751376104669 4 1.2845956215475824 3.4194123553756786 5 0.6052785277485605 2.013957584696591 6 0.33664876890807544 1.3441673089771373 7 0.21313574662793464 0.992840266650693 8 0.13338527589775795 0.7101055815222439 9 0.09427850715509165 0.5646511986427828 >10 0.4693707643828828 5.543968842562674 >50 0.036088977376358924 1.6645687464333945 >100 0.023497640670659126 3.11176336732995 >500 0.002331570794757892 1.0473152500825171 >1k 0.0018831917957659898 2.1025077430716377 >5k 1.793515995967609E-4 0.7935725467576817 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 7337 0.43778704229117105 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 5961 0.3556833254869389 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 4085 0.24374540926256424 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 3309 0.19744273176250307 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 2794 0.1667135063597563 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA 2253 0.13443290258716212 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 2175 0.12977876747761988 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 1794 0.10704510751947129 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.9668398840285E-5 0.0 5 0.0 0.0 0.0 5.9668398840285E-5 0.0 6 0.0 0.0 0.0 5.9668398840285E-5 0.0 7 0.0 0.0 0.0 5.9668398840285E-5 0.0 8 0.0 0.0 0.0 5.9668398840285E-5 0.0 9 0.0 0.0 0.0 5.9668398840285E-5 0.0 10-11 0.0 0.0 0.0 5.9668398840285E-5 0.0 12-13 0.0 0.0 0.0 8.950259826042751E-5 0.0 14-15 0.0 0.0 0.0 1.1933679768057E-4 0.0 16-17 0.0 0.0 0.0 1.79005196520855E-4 0.0 18-19 0.0 0.0 0.0 1.79005196520855E-4 0.0 20-21 0.0 0.0 0.0 2.3867359536114E-4 0.0 22-23 0.0 0.0 0.0 2.6850779478128253E-4 0.0 24-25 0.0 0.0 0.0 2.98341994201425E-4 0.0 26-27 0.0 0.0 0.0 4.17678791881995E-4 0.0 28-29 0.0 0.0 0.0 0.0012232021762258426 0.0 30-31 0.0 0.0 0.0 0.0047734719072228 0.0 32-33 0.0 0.0 0.0 0.011277327380813865 0.0 34-35 0.0 0.0 0.0 0.02067510019815875 0.0 36-37 0.0 0.0 0.0 0.03684523628387598 0.0 38-39 0.0 0.0 0.0 0.06250264778519854 0.0 40-41 0.0 0.0 0.0 0.09233684720534104 0.0 42-43 0.0 0.0 0.0 0.12258872541736553 0.0 44-45 0.0 0.0 0.0 0.15522733958300144 0.0 46-47 0.0 0.0 0.0 0.1931167728465824 0.0 48-49 0.0 0.0 0.0 0.23941945034664358 0.0 50-51 0.0 0.0 0.0 0.2885563767916183 0.0 52-53 0.0 0.0 0.0 0.3413629097652705 0.0 54-55 0.0 0.0 0.0 0.39685452068673555 0.0 56-57 0.0 0.0 0.0 0.46087871264236135 0.0 58-59 0.0 0.0 0.0 0.5367470817677837 0.0 60-61 0.0 0.0 0.0 0.6206110163378042 0.0 62-63 0.0 0.0 0.0 0.708920246621426 0.0 64-65 0.0 0.0 0.0 0.795379756540999 0.0 66-67 0.0 0.0 0.0 0.9039762424303177 0.0 68-69 0.0 0.0 0.0 1.0412433939623935 0.0 70-71 0.0 0.0 0.0 1.1933679768057002 0.0 72-73 2.98341994201425E-5 0.0 0.0 1.3293522577627095 0.0 74-75 5.9668398840285E-5 0.0 0.0 1.4676039378756498 0.0 76-77 5.9668398840285E-5 0.0 0.0 1.6273064073716728 0.0 78-79 5.9668398840285E-5 0.0 0.0 1.6736985874699943 0.0 80-81 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 82-83 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 84-85 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 86-87 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 88-89 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 90-91 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 92-93 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 94-95 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 96-97 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 98-99 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 100-101 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 102-103 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 104-105 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 106-107 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 108-109 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 110-111 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 112-113 5.9668398840285E-5 0.0 0.0 1.674712950250279 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGCT 40 0.0 3047.7876 110-111 TGTCGCG 10 8.7983836E-4 3047.7876 110-111 CGACGCC 25 7.366907E-10 2934.5989 108-109 CCGCTCC 315 0.0 2639.5864 108-109 TAGCGCG 10 0.0013670879 2445.499 108-109 ACGCTCG 10 0.0013670879 2445.499 108-109 CTCCGCC 125 0.0 2438.2302 110-111 CCGCGCC 220 0.0 2112.022 108-109 CCGTTCG 70 0.0 2096.142 108-109 CTCGTCG 15 0.001979421 2031.8584 110-111 CGCGGAC 15 0.0030755303 1630.3328 108-109 CGTGTCG 15 0.0030755303 1630.3328 108-109 CCGCGAC 15 0.0030755303 1630.3328 108-109 CGCGGCG 20 0.003518587 1523.8938 110-111 CGTTGCG 20 0.003518587 1523.8938 110-111 CGCTGTT 80 1.6370905E-11 1523.8938 110-111 CGGTGCG 60 1.0108124E-8 1523.8938 110-111 CGTCGAT 20 0.003518587 1523.8938 110-111 CAGCGCG 35 7.955799E-6 1397.4281 108-109 CCGCTCG 110 0.0 1333.9086 108-109 >>END_MODULE