FastQCFastQC Report
Fri 27 May 2016
SRR939918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675929
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA72290.43134285521642024No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC61070.3643949117176205No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA38340.22876864115365267No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA33440.19953112572191306No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA28330.16904057391452743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.15591352616966472No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA21830.13025611466834217No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA21680.12936108868573787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21540.1285257311019739No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA17520.10453903476817933No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCG505.3359644E-447.5951927
GGTATCA35650.043.6373251
GTATCAA55600.043.309561
CGACTCC558.5337844E-443.2683566
TAGGACC44700.040.1241954
ATCAACG65350.036.6545453
TATCAAC65950.036.592122
TCAACGC65650.036.577814
CAACGCA66400.035.985185
TACATGG64800.034.8507082
GTACATG65900.034.8219031
AACGCAG69100.034.5254556
ACATGGG62750.033.3304183
ACGCAGA79050.030.1044857
CGCAGAG79750.029.6910428
CATGGGG31900.029.5130274
GTAGGAC152050.028.6861363
TACAGTG146950.028.505795
TGTAGGA158500.028.3834422
TTCGACT1055.8568455E-428.374494
Produced by FastQC (version 0.11.5)