##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939918_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1675929 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.93449722512111 34.0 33.0 34.0 31.0 34.0 2 33.03301333171035 34.0 33.0 34.0 31.0 34.0 3 33.069022613726474 34.0 33.0 34.0 31.0 34.0 4 36.42195701607884 37.0 37.0 37.0 35.0 37.0 5 36.33253735689281 37.0 37.0 37.0 35.0 37.0 6 36.46784917499488 37.0 37.0 37.0 35.0 37.0 7 36.46461574446173 37.0 37.0 37.0 35.0 37.0 8 36.49501679367086 37.0 37.0 37.0 35.0 37.0 9 38.36041980298688 39.0 39.0 39.0 37.0 39.0 10-11 38.343645524362906 39.0 39.0 39.0 37.0 39.0 12-13 38.33845318029583 39.0 39.0 39.0 37.0 39.0 14-15 39.93570879195956 41.0 40.0 41.0 38.0 41.0 16-17 39.95375311245286 41.0 40.0 41.0 38.0 41.0 18-19 39.982771048176865 41.0 40.0 41.0 38.0 41.0 20-21 39.99542134541499 41.0 40.0 41.0 38.0 41.0 22-23 39.95258928033347 41.0 40.0 41.0 38.0 41.0 24-25 39.941573598881575 41.0 40.0 41.0 38.0 41.0 26-27 39.86755405509422 41.0 40.0 41.0 38.0 41.0 28-29 39.79560560143061 41.0 40.0 41.0 38.0 41.0 30-31 39.681156838983036 41.0 40.0 41.0 37.0 41.0 32-33 39.57622697620245 41.0 40.0 41.0 37.0 41.0 34-35 39.53069103762749 41.0 40.0 41.0 36.0 41.0 36-37 39.51588790455921 41.0 40.0 41.0 36.5 41.0 38-39 39.54961487031969 41.0 40.0 41.0 37.0 41.0 40-41 39.43614019448318 41.0 40.0 41.0 36.5 41.0 42-43 39.392591511931585 41.0 40.0 41.0 36.5 41.0 44-45 39.303952315402384 41.0 40.0 41.0 36.0 41.0 46-47 39.11876040094777 41.0 40.0 41.0 36.0 41.0 48-49 38.940566694651146 41.0 40.0 41.0 36.0 41.0 50-51 38.83042241049591 41.0 40.0 41.0 36.0 41.0 52-53 38.761318349405016 41.0 40.0 41.0 36.0 41.0 54-55 38.69280590048862 41.0 39.5 41.0 35.5 41.0 56-57 38.57278112616943 41.0 39.0 41.0 35.0 41.0 58-59 38.45210745801284 41.0 39.0 41.0 35.0 41.0 60-61 38.309928105546234 41.0 39.0 41.0 35.0 41.0 62-63 38.131431582125494 40.5 38.5 41.0 35.0 41.0 64-65 37.924587199099726 40.0 37.5 41.0 34.0 41.0 66-67 37.673138599546874 40.0 37.0 41.0 34.0 41.0 68-69 37.332529898342955 39.0 37.0 41.0 34.0 41.0 70-71 36.9463724298583 39.0 36.0 41.0 33.5 41.0 72-73 36.607735470894056 39.0 35.5 40.5 33.0 41.0 74-75 36.24653908369626 37.5 35.0 40.0 33.0 41.0 76-77 35.83828282701714 37.0 35.0 39.0 32.0 41.0 78-79 35.427233492588286 36.5 35.0 39.0 32.0 40.5 80-81 35.10926089351041 36.0 35.0 39.0 32.0 39.5 82-83 34.81620999457614 36.0 35.0 37.0 32.0 39.0 84-85 34.481769514102325 35.0 35.0 37.0 32.0 39.0 86-87 34.21569380325778 35.0 35.0 36.5 32.0 38.0 88-89 33.94791933309824 35.0 35.0 36.0 32.0 37.0 90-91 33.71179894852348 35.0 35.0 36.0 31.5 37.0 92-93 33.57207912745707 35.0 35.0 36.0 31.0 36.5 94-95 33.46257657693136 35.0 35.0 35.0 31.0 36.0 96-97 33.293881781388116 35.0 35.0 35.0 31.0 36.0 98-99 33.15451877734677 35.0 34.0 35.0 31.0 36.0 100-101 33.16975271625469 35.0 35.0 35.0 31.0 36.0 102-103 32.37429360074323 34.5 33.0 35.0 29.5 35.0 104-105 33.0129396889725 35.0 34.0 35.0 31.0 35.0 106-107 33.069540237086414 35.0 34.0 35.0 31.0 35.5 108-109 33.06806165416315 35.0 35.0 35.0 31.0 35.5 110-111 33.05973105065907 35.0 35.0 35.0 31.0 36.0 112-113 32.98588126346641 35.0 34.5 35.0 31.0 36.0 114-115 32.91404707478658 35.0 34.0 35.0 31.0 36.0 116-117 32.854945525735275 35.0 34.0 35.0 31.0 35.5 118-119 32.774162867281376 35.0 34.0 35.0 31.0 35.0 120-121 32.68053688431908 35.0 34.0 35.0 30.5 35.0 122-123 32.52149046886831 35.0 34.0 35.0 30.0 35.0 124-125 30.472372039626975 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.036579601207769485 1101 2 0.08110458666015319 1101 3 0.0976883699162272 1101 4 0.04807978106941846 1101 5 -0.019728110047466885 1101 6 0.015900469007220863 1101 7 0.060108575722658486 1101 8 0.08665921892985295 1101 9 0.12403070632630175 1101 10-11 0.09760963001118483 1101 12-13 0.03989824246443874 1101 14-15 0.14965951662331634 1101 16-17 0.13365562096444705 1101 18-19 0.18631213680409076 1101 20-21 0.15961802954063842 1101 22-23 0.151153007979012 1101 24-25 0.19910963180863916 1101 26-27 0.2083453125987944 1101 28-29 0.1780383989678569 1101 30-31 0.2265810045846024 1101 32-33 0.237959551419479 1101 34-35 0.2342886887330593 1101 36-37 0.22994396251181115 1101 38-39 0.18567055271549293 1101 40-41 0.257705388421563 1101 42-43 0.22583227203514156 1101 44-45 0.24204749651709534 1101 46-47 0.362378539685146 1101 48-49 0.30953852707202145 1101 50-51 0.33805190499863613 1101 52-53 0.32480123413905915 1101 54-55 0.2874186339939868 1101 56-57 0.3571481938838019 1101 58-59 0.39222372589591714 1101 60-61 0.3369981120954577 1101 62-63 0.2714287418184895 1101 64-65 0.2754143982816615 1101 66-67 0.3636593747268435 1101 68-69 0.35896902033780265 1101 70-71 0.3440354029845949 1101 72-73 0.35329559301654 1101 74-75 0.3023614809946835 1101 76-77 0.3730086714368426 1101 78-79 0.3284009304210187 1101 80-81 0.33958512055346546 1101 82-83 0.3009190628197871 1101 84-85 0.3323829150378188 1101 86-87 0.28056046558153014 1101 88-89 0.296776643733061 1101 90-91 0.3372262188222521 1101 92-93 0.3688374119886575 1101 94-95 0.3486673115621599 1101 96-97 0.34112830223951107 1101 98-99 0.45441683106591313 1101 100-101 0.4353840427457456 1101 102-103 0.4240433208650458 1101 104-105 0.4150747467508822 1101 106-107 0.41434406354078845 1101 108-109 0.4736303520065519 1101 110-111 0.47038653191881963 1101 112-113 0.4947688222783455 1101 114-115 0.5080890853201296 1101 116-117 0.5084993227821144 1101 118-119 0.5240352455383146 1101 120-121 0.5061763679913156 1101 122-123 0.5003676937772141 1101 124-125 0.6729324857709287 1102 1 0.02598288609492272 1102 2 0.10949168343434934 1102 3 0.1263826863524784 1102 4 0.0856787412490192 1102 5 -0.07722072495943166 1102 6 -0.01432049282664849 1102 7 -0.013104232974022523 1102 8 0.005737559943675308 1102 9 0.06681695233834972 1102 10-11 0.1771700695716234 1102 12-13 0.09535611792231435 1102 14-15 0.12340877811451634 1102 16-17 0.17134438381449968 1102 18-19 0.08454444213317203 1102 20-21 0.045927848753677836 1102 22-23 0.16837148842329697 1102 24-25 0.17582710788096279 1102 26-27 0.16047812602193545 1102 28-29 0.12702575569714725 1102 30-31 0.10966974380237815 1102 32-33 0.157898107487064 1102 34-35 0.16736443019589586 1102 36-37 0.18598791855576735 1102 38-39 0.12059670183518278 1102 40-41 0.07905715493461685 1102 42-43 -0.01181158947969152 1102 44-45 0.012266095122789977 1102 46-47 0.09354951924321853 1102 48-49 0.10327597242236664 1102 50-51 0.06373074229037456 1102 52-53 0.06913539460224882 1102 54-55 0.04587780922735618 1102 56-57 0.08604338471447193 1102 58-59 0.13993257618541577 1102 60-61 0.09028418086539602 1102 62-63 0.06657939761431209 1102 64-65 0.14614345417200525 1102 66-67 0.16052809740201468 1102 68-69 0.106531350480779 1102 70-71 0.14851135713796992 1102 72-73 0.16935136520133653 1102 74-75 0.2395917775798182 1102 76-77 0.1873629193394848 1102 78-79 0.20593135698370446 1102 80-81 0.22834324415030238 1102 82-83 0.143289380673977 1102 84-85 0.11714245657155686 1102 86-87 0.12231398088343326 1102 88-89 0.14445949851268836 1102 90-91 0.23088331441483945 1102 92-93 0.16712170833746853 1102 94-95 0.145448594795063 1102 96-97 0.2116233985410645 1102 98-99 0.21902276276861699 1102 100-101 0.22126848088824858 1102 102-103 0.13569876219791155 1102 104-105 0.2787968311181217 1102 106-107 0.1863090877638811 1102 108-109 0.08426015231377448 1102 110-111 0.08046333683432749 1102 112-113 0.1358535440082207 1102 114-115 0.1114826809717897 1102 116-117 0.12865293261315003 1102 118-119 0.1925499566336697 1102 120-121 0.21665964815201733 1102 122-123 0.2190088375796435 1102 124-125 0.2895294372065855 1103 1 -0.02703831933322931 1103 2 0.0363370465080024 1103 3 0.009319815393823205 1103 4 -0.06896163904892916 1103 5 -0.14970275079133444 1103 6 0.005266487604004055 1103 7 -0.08051695259518254 1103 8 -0.024938414206239656 1103 9 -0.02547056163651007 1103 10-11 0.014101599580079949 1103 12-13 0.027100271204936632 1103 14-15 -0.009824845168736829 1103 16-17 0.021035164497831715 1103 18-19 0.0021363126282665235 1103 20-21 0.05761464830648322 1103 22-23 0.015041427252043604 1103 24-25 0.07041986004195167 1103 26-27 0.11223373187183228 1103 28-29 0.07619562635585453 1103 30-31 -0.006504371577690904 1103 32-33 -0.0593270081747761 1103 34-35 -0.003175385654095919 1103 36-37 0.033959178065487094 1103 38-39 0.020598701489788596 1103 40-41 0.03752787362866172 1103 42-43 0.0326961773605845 1103 44-45 -0.08786797262914092 1103 46-47 -0.07935434281696274 1103 48-49 -0.03507685754337331 1103 50-51 0.008127982767021535 1103 52-53 -0.0017583601128450255 1103 54-55 0.029422015228128373 1103 56-57 0.02777372220162988 1103 58-59 0.06061763967444733 1103 60-61 0.051943167040512606 1103 62-63 0.03013541975594336 1103 64-65 -0.0038755425467442706 1103 66-67 -0.04007688900941986 1103 68-69 0.029450846204241543 1103 70-71 0.1263430952922917 1103 72-73 0.06134293029464288 1103 74-75 0.012446011848446403 1103 76-77 0.06390469848671643 1103 78-79 0.06124116710741134 1103 80-81 -0.04701673912531845 1103 82-83 0.05666716087557688 1103 84-85 -0.03617160736285285 1103 86-87 -0.04968467389437592 1103 88-89 -0.01556486091613607 1103 90-91 0.020235517216171672 1103 92-93 -0.020858276937794074 1103 94-95 0.012369762956922159 1103 96-97 0.07573523376952096 1103 98-99 0.11078200435416363 1103 100-101 0.0754178550872524 1103 102-103 -0.05297273999716623 1103 104-105 -0.039811136596540564 1103 106-107 -0.029620433500625154 1103 108-109 -0.05264183734256278 1103 110-111 -0.052377626999188465 1103 112-113 -0.03889136368953672 1103 114-115 -0.041961633192123315 1103 116-117 4.6043858938560334E-4 1103 118-119 -0.015170164436320022 1103 120-121 -0.021093285432186804 1103 122-123 -0.0017540306522008109 1103 124-125 -0.05370318624935777 1104 1 0.0634201473063527 1104 2 0.07528430636337191 1104 3 0.03790978082189156 1104 4 0.0022352216716825524 1104 5 0.033724323751222585 1104 6 0.034747755274125325 1104 7 0.08270390290165608 1104 8 0.0734640711262955 1104 9 0.02116740310025733 1104 10-11 0.05947839603494032 1104 12-13 0.04276199865878283 1104 14-15 0.056428040762000364 1104 16-17 0.035878064637842044 1104 18-19 0.07563947887668832 1104 20-21 0.11669321321927839 1104 22-23 0.13425257947099567 1104 24-25 0.10717301113335509 1104 26-27 0.12778082350174458 1104 28-29 0.15916565588241838 1104 30-31 0.10565667746902108 1104 32-33 0.12821040526472416 1104 34-35 0.09617711358109204 1104 36-37 0.1195410925046474 1104 38-39 0.09658726996362077 1104 40-41 0.1394990523009625 1104 42-43 0.21565652033424243 1104 44-45 0.226066412473358 1104 46-47 0.20633032573915244 1104 48-49 0.08965702357963323 1104 50-51 0.12038659421708076 1104 52-53 0.0773372224705895 1104 54-55 0.09627316775371497 1104 56-57 0.21395676042031653 1104 58-59 0.16568938636899588 1104 60-61 0.14945818293970348 1104 62-63 0.060080979934873824 1104 64-65 0.035774689268500026 1104 66-67 0.051880205833079174 1104 68-69 0.01356873224996491 1104 70-71 0.1096693321222375 1104 72-73 0.12082881824143499 1104 74-75 0.02871454260743178 1104 76-77 0.03517204804374785 1104 78-79 0.033475081331935996 1104 80-81 0.015444627905147001 1104 82-83 0.0366623385053515 1104 84-85 0.06563894178107432 1104 86-87 0.05415168886771937 1104 88-89 0.013526505577722503 1104 90-91 0.011052804437795771 1104 92-93 -0.01735665775033368 1104 94-95 0.0014866768629815397 1104 96-97 -0.006744196243047895 1104 98-99 -0.056089949869559064 1104 100-101 0.09776556888655108 1104 102-103 0.03677979296673328 1104 104-105 0.0474014637272262 1104 106-107 0.05839287540494098 1104 108-109 -0.010047660006165415 1104 110-111 -0.03795877487661414 1104 112-113 0.06013830852169377 1104 114-115 0.07496795253700128 1104 116-117 0.01806592271145746 1104 118-119 0.06653155384665865 1104 120-121 -0.023325548064605073 1104 122-123 0.004897286196332118 1104 124-125 -0.05147580173711219 1105 1 0.020622471917306484 1105 2 0.01789471011694843 1105 3 -0.05856089522193031 1105 4 0.04803636769266717 1105 5 -0.061529765182449125 1105 6 0.07877389188064399 1105 7 -0.009966041018628857 1105 8 0.03754693203978832 1105 9 0.03083394424064778 1105 10-11 0.06786965804899836 1105 12-13 0.07301043890996795 1105 14-15 0.059329394070971375 1105 16-17 0.08363165316269772 1105 18-19 0.04901145394784834 1105 20-21 0.06343614558097244 1105 22-23 0.08370535275357582 1105 24-25 0.07293317452107573 1105 26-27 0.11173924377050781 1105 28-29 0.11603279140669542 1105 30-31 0.014446061338546201 1105 32-33 0.10111063901500472 1105 34-35 0.12539708650812287 1105 36-37 0.1475105523006235 1105 38-39 0.17846076661681565 1105 40-41 0.16035676996183668 1105 42-43 0.15148324657500467 1105 44-45 0.08713002715518314 1105 46-47 0.15125295744845602 1105 48-49 0.19351492104841128 1105 50-51 0.19742648103987648 1105 52-53 0.1654754981466624 1105 54-55 0.06823941814810297 1105 56-57 0.13838773623444922 1105 58-59 0.08936839192946877 1105 60-61 0.19294845804580518 1105 62-63 0.1278683927025881 1105 64-65 0.13727846014572265 1105 66-67 0.01714962932820896 1105 68-69 0.03358033494912149 1105 70-71 0.08159113942922858 1105 72-73 -0.00628383989622705 1105 74-75 -0.06268487182944682 1105 76-77 -0.0014529574692332403 1105 78-79 0.037022555709313565 1105 80-81 -0.05493167951889433 1105 82-83 0.09163267020005605 1105 84-85 -0.014487704055021311 1105 86-87 -0.03806568193351012 1105 88-89 0.035181654360293635 1105 90-91 0.06211655482370304 1105 92-93 -0.05695103824422887 1105 94-95 0.01924389547207994 1105 96-97 0.02178402511745503 1105 98-99 -0.027868250108056714 1105 100-101 0.05740547810051311 1105 102-103 0.008325869592034962 1105 104-105 0.035764838552083233 1105 106-107 0.005079829832112637 1105 108-109 0.06433581937868382 1105 110-111 0.010974421395587797 1105 112-113 -0.04343359259074475 1105 114-115 0.07745506913055777 1105 116-117 -0.023905145177906206 1105 118-119 0.010833509003241204 1105 120-121 -0.18058978766372746 1105 122-123 -0.10195492187873612 1105 124-125 -0.13246976629799434 1106 1 0.009952171540533072 1106 2 0.02624330801118191 1106 3 -0.01454782975061164 1106 4 0.011895844378841502 1106 5 -0.060919182746374645 1106 6 0.013134790777122873 1106 7 -0.020780796264425305 1106 8 -0.034195511382058896 1106 9 -0.0025688862805282042 1106 10-11 0.022469627271668458 1106 12-13 -0.010643077895934994 1106 14-15 -0.07695881076702449 1106 16-17 -0.02418280920208815 1106 18-19 0.01567042628300186 1106 20-21 0.03815530248080279 1106 22-23 0.00959280702194576 1106 24-25 0.006210910104130107 1106 26-27 -0.005597077941907003 1106 28-29 0.05295490987954565 1106 30-31 -0.032024226131596834 1106 32-33 -0.010309734582023111 1106 34-35 0.034232661125763286 1106 36-37 0.041878549838429535 1106 38-39 -0.043279194762604334 1106 40-41 -0.06488982095289941 1106 42-43 -0.10248826950615353 1106 44-45 -0.1258676844017046 1106 46-47 -0.02905551078388413 1106 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2215 14-15 -0.07323103401243003 2215 16-17 -0.047682764276871126 2215 18-19 -0.04363086256257276 2215 20-21 -0.033149964177809466 2215 22-23 -0.004641982335698458 2215 24-25 -0.06621057545292075 2215 26-27 -0.07560154607000413 2215 28-29 -0.028966938128483832 2215 30-31 -0.06182834811190219 2215 32-33 0.02059617330786523 2215 34-35 -0.06863978825002448 2215 36-37 -0.13585232411359982 2215 38-39 0.009955563289679503 2215 40-41 -0.024540266159789326 2215 42-43 -0.09396691091874487 2215 44-45 -0.10898921870566625 2215 46-47 -0.07003137598058373 2215 48-49 -0.08592371880479277 2215 50-51 -0.07914606386768241 2215 52-53 -0.09002700469322633 2215 54-55 -0.07688222860819138 2215 56-57 -0.06147349450533568 2215 58-59 -0.09171446600991828 2215 60-61 -0.033207779081109834 2215 62-63 -0.05283893522976513 2215 64-65 -0.07634173234201569 2215 66-67 -0.10925543543770999 2215 68-69 -0.07811872508159468 2215 70-71 -0.11028638577601413 2215 72-73 -0.0633735551591883 2215 74-75 -0.017410516471954907 2215 76-77 -0.03803582165011221 2215 78-79 -0.043781723460320165 2215 80-81 -0.06698754506874138 2215 82-83 -0.02580419706829673 2215 84-85 -0.08387171001356108 2215 86-87 -0.023934102876637553 2215 88-89 -0.02910845869400447 2215 90-91 -0.03655941583294009 2215 92-93 -0.08914301001603064 2215 94-95 -0.03683719404344998 2215 96-97 -0.018072503695364617 2215 98-99 -0.10326780987453787 2215 100-101 -0.05201418760496068 2215 102-103 -0.015030224396568315 2215 104-105 -0.09060464968685977 2215 106-107 -0.1038862871668087 2215 108-109 -0.061635669467762 2215 110-111 -0.10907035093543982 2215 112-113 -0.14421190713717635 2215 114-115 -0.10537936062556952 2215 116-117 -0.06332310487448467 2215 118-119 -0.08769162550098741 2215 120-121 -0.08169994174546247 2215 122-123 -0.08657712412817631 2215 124-125 0.0034537878594704807 2216 1 -0.08027670704094447 2216 2 -0.20118038575139963 2216 3 -0.12742221570069034 2216 4 -0.06946276077692204 2216 5 -0.0056436291275403505 2216 6 -0.06431096512826429 2216 7 -0.050135779078658516 2216 8 -0.06938844648490061 2216 9 -0.0477428365864867 2216 10-11 -0.059642653570207926 2216 12-13 -0.08528653056843183 2216 14-15 -0.10399560462673918 2216 16-17 -0.09175913305790573 2216 18-19 -0.20973831063876247 2216 20-21 -0.20979264417383803 2216 22-23 -0.1737637630137101 2216 24-25 -0.20983737055608032 2216 26-27 -0.20925101512411715 2216 28-29 -0.23335493343813596 2216 30-31 -0.24746545528464026 2216 32-33 -0.33562787381352166 2216 34-35 -0.21561533129754196 2216 36-37 -0.19333697595899935 2216 38-39 -0.1884296930303293 2216 40-41 -0.2614108539858222 2216 42-43 -0.18818080349692679 2216 44-45 -0.15283635380978922 2216 46-47 -0.19227347496686775 2216 48-49 -0.21219931314684004 2216 50-51 -0.21347125538528644 2216 52-53 -0.23207640299174415 2216 54-55 -0.304969737128566 2216 56-57 -0.3752948904580222 2216 58-59 -0.3439917885474486 2216 60-61 -0.3615125755291473 2216 62-63 -0.34617222261791625 2216 64-65 -0.3958240678935141 2216 66-67 -0.3462390812475604 2216 68-69 -0.4179902229505572 2216 70-71 -0.44523616932495 2216 72-73 -0.4053690334961928 2216 74-75 -0.4116358297114857 2216 76-77 -0.4724301934956898 2216 78-79 -0.506541342495936 2216 80-81 -0.4748953356991379 2216 82-83 -0.3359010790636532 2216 84-85 -0.33310138968698766 2216 86-87 -0.3024515435064288 2216 88-89 -0.3871617807117005 2216 90-91 -0.39899115030650734 2216 92-93 -0.37895968542868985 2216 94-95 -0.323547646475582 2216 96-97 -0.34661408195856325 2216 98-99 -0.3718909539760489 2216 100-101 -0.42577699031402005 2216 102-103 -0.46323539198328945 2216 104-105 -0.4392452792147168 2216 106-107 -0.4356601095013488 2216 108-109 -0.4573484582127634 2216 110-111 -0.4881760868969707 2216 112-113 -0.582974025591092 2216 114-115 -0.6054426993508955 2216 116-117 -0.6706512313051434 2216 118-119 -0.734490279569485 2216 120-121 -0.7363348760389172 2216 122-123 -0.7668340496270645 2216 124-125 -0.9128068381962464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 163.0 3 7.0 4 11.0 5 34.0 6 60.0 7 203.0 8 372.0 9 738.0 10 1716.0 11 2901.0 12 3440.0 13 5937.0 14 10168.0 15 11300.0 16 3715.0 17 2342.0 18 1946.0 19 1782.0 20 1944.0 21 2069.0 22 2157.0 23 2263.0 24 2780.0 25 3723.0 26 4678.0 27 6087.0 28 8010.0 29 10244.0 30 13335.0 31 17341.0 32 22694.0 33 30533.0 34 44286.0 35 70658.0 36 152920.0 37 564373.0 38 661321.0 39 7678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.10502174376198 20.643713227022538 14.81392627232488 24.437338756890597 2 12.170205301059891 19.877990058051385 47.341742997465886 20.610061643422842 3 18.93314096241547 25.699477722504948 29.562887210615724 25.804494104463853 4 11.097307821512725 17.412372481173126 40.40887173621317 31.081447961100977 5 10.944881466257819 39.49809988639358 36.36050703959372 13.196511607754877 6 29.025931289452 38.3966146537234 19.578454695873155 12.99899936095145 7 27.2712030163569 32.018719170084175 22.863021046834323 17.847056766724606 8 22.762300789591922 41.65832800792874 20.65350023777857 14.92587096470077 9 25.715349516596465 17.138613867293902 22.859142600909703 34.28689401519993 10-11 23.395859848478068 26.0405124560766 31.903917170715467 18.659710524729867 12-13 27.14611631701472 24.34023885863866 29.98072411800384 18.532920706342782 14-15 19.132851093333905 31.748003644545804 26.382531718229114 22.736613543891178 16-17 18.05819878353999 30.89669398187333 32.83985563823278 18.205251596353904 18-19 20.073105722259115 27.297606282843724 33.97649900443277 18.652788990464394 20-21 20.671104776731518 28.38691814697319 30.880498322208222 20.061478754087073 22-23 18.97672001334189 31.985450418560024 31.326828863710748 17.711000704387338 24-25 20.229586098217762 28.503534457605305 33.8999742829201 17.36690516125683 26-27 17.291185963128513 31.247027767882766 32.897336343007375 18.564449925981354 28-29 17.91063344568893 30.661501770063055 30.052884101892147 21.374980682355876 30-31 19.50374986052512 28.3676993476454 32.32102911280848 19.807521679021008 32-33 17.733627140529222 30.629489674085235 33.5239141992292 18.112968986156336 34-35 18.00562553664266 30.931918953607223 33.399624924444886 17.662830585305226 36-37 17.332088650533525 29.601194322671187 32.874930859242845 20.191786167552443 38-39 19.724522936234173 30.78194243312217 30.794562299476887 18.698972331166775 40-41 22.333344670329115 27.222543437102647 32.8821209013031 17.56199099126514 42-43 18.111029763194026 31.314244219176352 33.03233609538351 17.54238992224611 44-45 17.490985596645203 31.415889336600777 31.50342287769947 19.58970218905455 46-47 20.516620930838954 28.17195716524984 31.359144689303665 19.952277214607538 48-49 19.67347662102631 29.52887622327676 30.35265813766574 20.444989018031194 50-51 17.944674267227313 32.07659751695925 30.354269184434422 19.62445903137901 52-53 17.91576492798919 30.470234717580517 32.00380207037411 19.610198284056185 54-55 18.33186250730192 29.201505553039535 32.46387525963212 20.00275668002642 56-57 19.168711801036917 29.604177742613203 29.200670195455775 22.026440260894105 58-59 19.79257474511152 30.622776979215704 28.284491765462615 21.300156510210158 60-61 18.72743415741359 27.941368637931557 32.78966471730008 20.541532487354775 62-63 19.77204881591046 28.12550531675268 31.956306024897234 20.146139842439624 64-65 20.381770349459913 29.649615228330077 29.49513374373258 20.47348067847743 66-67 20.10604267841895 31.793053285670215 28.38738991926269 19.71351411664814 68-69 21.40472537917776 28.16175386904815 31.9067812538598 18.526739497914292 70-71 22.43916657567236 28.37727612565926 30.276998607936257 18.906558690732126 72-73 20.594189849331325 30.542821324769726 29.4867204994961 19.376268326402847 74-75 19.036307624010327 31.09690207640061 31.85665979883396 18.010130500755103 76-77 19.93067725422736 28.44141965441257 32.5696076623771 19.05829542898297 78-79 19.14672399606427 28.603180683668576 31.20851181643137 21.041583503835785 80-81 20.045061574804183 29.96203896465781 30.849725734204732 19.143173726333277 82-83 19.14603780947761 29.86066235502817 32.4520012482629 18.54129858723132 84-85 18.852409618784566 29.173521073983444 32.42443444799869 19.549634859233297 86-87 17.368068695034218 32.04219868502783 29.415088586688338 21.174644033249617 88-89 19.586927608508475 28.95095794630918 31.11578712463356 20.34632732054878 90-91 19.52356204010225 29.681589053389313 32.206506770029044 18.588342136479394 92-93 17.988053193184196 31.52520184327618 31.906811088059218 18.579933875480407 94-95 18.0265544255934 31.2318065965697 31.142542006120234 19.59909697171667 96-97 20.472585652494825 29.75063382756668 30.417368516208025 19.35941200373047 98-99 20.387581056830403 28.957833293305267 32.314760808696185 18.33982484116814 100-101 19.167524151537492 29.559703770178743 32.27076811451085 19.002003963772918 102-103 18.128390475357957 31.032983845055256 31.222968310364447 19.615657369222337 104-105 19.12165764512167 29.46314373420061 31.170499592605864 20.244699028071857 106-107 19.3512709071711 28.451534925519017 30.705521829325814 21.491672337984063 108-109 18.41521140240595 30.465945115747882 30.15823481571691 20.96060866612925 110-111 18.024894680939934 30.574182807633093 31.466590287971545 19.93433222345542 112-113 18.931087396351526 28.482605137318284 30.81662958479744 21.76967788153275 114-115 18.69544008198812 29.44939963391766 28.920236118632157 22.934924165462057 116-117 19.423470739443932 29.408416213777844 27.54927478119324 23.618838265584984 118-119 19.658081664082523 27.95579042398339 30.056867052343883 22.329260859590203 120-121 18.64471656654768 27.765156205015444 31.423648766574825 22.16647846186205 122-123 19.939609217157404 29.05849212192959 29.635907007383416 21.365991653529587 124-125 20.737510870975875 29.14975916033552 28.32005943048766 21.792670538200944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76.0 1 46.0 2 39.5 3 60.5 4 41.0 5 38.0 6 91.5 7 209.5 8 343.0 9 407.5 10 351.5 11 312.5 12 379.0 13 475.0 14 589.5 15 727.5 16 896.0 17 1149.0 18 1414.0 19 1743.0 20 2239.5 21 2781.5 22 3436.0 23 4400.5 24 5662.5 25 7223.0 26 9376.5 27 12130.0 28 15135.5 29 19032.0 30 24758.0 31 33344.0 32 46284.0 33 64632.0 34 88632.0 35 114055.0 36 132231.0 37 134331.0 38 119924.0 39 97763.5 40 79407.5 41 68336.5 42 59197.0 43 52614.5 44 49030.0 45 46014.5 46 43772.5 47 42349.5 48 41479.5 49 39013.0 50 35174.0 51 31695.5 52 28172.0 53 24408.0 54 20496.5 55 16782.0 56 13176.0 57 10178.0 58 7777.5 59 5713.0 60 4136.5 61 2959.5 62 2165.0 63 1498.0 64 957.5 65 682.5 66 522.0 67 396.5 68 311.5 69 229.0 70 159.0 71 106.0 72 71.0 73 73.0 74 66.5 75 38.5 76 17.5 77 5.0 78 4.0 79 1.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.5 98 5.0 99 6.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01646847807991866 2 0.0 3 0.0 4 0.0 5 0.15561518417546327 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.98341994201425E-5 14-15 0.0 16-17 2.98341994201425E-5 18-19 0.0 20-21 0.0039082801240386674 22-23 2.6850779478128253E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.756891849237049E-4 92-93 0.0 94-95 7.458549855035626E-4 96-97 0.0 98-99 0.0012828705750661275 100-101 2.98341994201425E-5 102-103 1.491709971007125E-4 104-105 0.001998891361149548 106-107 0.005877337285768072 108-109 1.1933679768057E-4 110-111 0.004117119519979665 112-113 0.007906062846337762 114-115 0.004564632511281802 116-117 0.001253036375645985 118-119 1.491709971007125E-4 120-121 3.8784459246185245E-4 122-123 6.56352387243135E-4 124-125 0.0012828705750661275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1675929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.20239984488761 #Duplication Level Percentage of deduplicated Percentage of total 1 81.00270270584967 52.815706103433705 2 12.276166284817188 16.008710053299577 3 3.435734127237464 6.720543309745894 4 1.2728235508819068 3.3196460038636695 5 0.6237000484435968 2.0333369970947586 6 0.3638183942852658 1.4233099449067725 7 0.21551795797809994 0.983660164888923 8 0.14246980905992582 0.743149876492006 9 0.10579027449018846 0.6208001799308713 >10 0.49174222290088965 5.79167154793459 >50 0.03729335321979888 1.6750298644523554 >100 0.027020919634361446 3.4159157139204157 >500 0.0028391383728244755 1.2112133167146653 >1k 0.0021980426112189486 2.4409384969693173 >5k 1.8317021760157903E-4 0.7963684263524516 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 7229 0.43134285521642024 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 6107 0.3643949117176205 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 3834 0.22876864115365267 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 3344 0.19953112572191306 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 2833 0.16904057391452743 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.15591352616966472 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 2183 0.13025611466834217 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA 2168 0.12936108868573787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2154 0.1285257311019739 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 1752 0.10453903476817933 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.9668398840285E-5 0.0 6 0.0 0.0 0.0 5.9668398840285E-5 0.0 7 0.0 0.0 0.0 5.9668398840285E-5 0.0 8 0.0 0.0 0.0 5.9668398840285E-5 0.0 9 0.0 0.0 0.0 5.9668398840285E-5 0.0 10-11 0.0 0.0 0.0 5.9668398840285E-5 0.0 12-13 0.0 0.0 0.0 1.1933679768057E-4 0.0 14-15 0.0 0.0 0.0 1.1933679768057E-4 0.0 16-17 0.0 0.0 0.0 1.79005196520855E-4 0.0 18-19 5.9668398840285E-5 0.0 0.0 2.98341994201425E-4 0.0 20-21 5.9668398840285E-5 0.0 0.0 2.98341994201425E-4 0.0 22-23 5.9668398840285E-5 0.0 0.0 3.2817619362156747E-4 0.0 24-25 5.9668398840285E-5 0.0 0.0 4.7734719072228E-4 0.0 26-27 5.9668398840285E-5 0.0 0.0 6.563523872431349E-4 0.0 28-29 5.9668398840285E-5 0.0 0.0 0.0014917099710071249 0.0 30-31 5.9668398840285E-5 0.0 0.0 0.00501214550258394 0.0 32-33 5.9668398840285E-5 0.0 0.0 0.01157566937501529 0.0 34-35 5.9668398840285E-5 0.0 0.0 0.020973442192360178 0.0 36-37 5.9668398840285E-5 0.0 0.0 0.037054075679816986 0.0 38-39 5.9668398840285E-5 0.0 0.0 0.06277115557997982 0.0 40-41 5.9668398840285E-5 0.0 0.0 0.09272469179780288 0.0 42-43 5.9668398840285E-5 0.0 0.0 0.12294673581040724 0.0 44-45 5.9668398840285E-5 0.0 0.0 0.15540634477952228 0.0 46-47 5.9668398840285E-5 0.0 0.0 0.19314660704600253 0.0 48-49 5.9668398840285E-5 0.0 0.0 0.23924044515012272 0.0 50-51 5.9668398840285E-5 0.0 0.0 0.2882282005979967 0.0 52-53 5.9668398840285E-5 0.0 0.0 0.34094523097338847 0.0 54-55 5.9668398840285E-5 0.0 0.0 0.39652634449311397 0.0 56-57 5.9668398840285E-5 0.0 0.0 0.46010302345743764 0.0 58-59 5.9668398840285E-5 0.0 0.0 0.5354045427938773 0.0 60-61 5.9668398840285E-5 0.0 0.0 0.619089472167377 0.0 62-63 1.1933679768057E-4 0.0 0.0 0.7069511894596967 0.0 64-65 1.1933679768057E-4 0.0 0.0 0.793291362581589 0.0 66-67 1.491709971007125E-4 0.0 0.0 0.9014403354796057 0.0 68-69 1.79005196520855E-4 0.0 0.0 1.038200305621539 0.0 70-71 1.79005196520855E-4 0.0 0.0 1.1890121836903593 0.0 72-73 1.79005196520855E-4 0.0 0.0 1.324817459450848 0.0 74-75 1.79005196520855E-4 0.0 0.0 1.4623232845782845 0.0 76-77 1.79005196520855E-4 0.0 0.0 1.6207428834992412 0.0 78-79 1.79005196520855E-4 0.0 0.0 1.7963768154856203 0.0 80-81 1.79005196520855E-4 0.0 0.0 1.9764560431856004 0.0 82-83 1.79005196520855E-4 0.0 0.0 2.1668877380843696 0.0 84-85 1.79005196520855E-4 0.0 0.0 2.354694023434167 0.0 86-87 1.79005196520855E-4 0.0 0.0 2.548407480269152 0.0 88-89 1.79005196520855E-4 0.0 0.0 2.758828088779417 0.0 90-91 2.088393959409975E-4 0.0 0.0 2.992012191447251 0.0 92-93 2.3867359536114E-4 0.0 0.0 3.2343255591376483 0.0 94-95 2.3867359536114E-4 0.0 0.0 3.4733869990912503 0.0 96-97 2.3867359536114E-4 0.0 0.0 3.7183556105300406 0.0 98-99 2.3867359536114E-4 0.0 0.0 3.9965893543222895 0.0 100-101 2.3867359536114E-4 0.0 0.0 4.290575555408374 0.0 102-103 2.3867359536114E-4 0.0 0.0 4.599866700796991 0.0 104-105 2.3867359536114E-4 0.0 0.0 4.906830778630837 0.0 106-107 2.3867359536114E-4 0.0 0.0 5.224863344449556 0.0 108-109 2.3867359536114E-4 0.0 0.0 5.566047249018306 0.0 110-111 2.3867359536114E-4 0.0 0.0 5.934380275059384 0.0 112-113 2.3867359536114E-4 0.0 0.0 6.348777305005164 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTCG 50 5.3359644E-4 47.595192 7 GGTATCA 3565 0.0 43.637325 1 GTATCAA 5560 0.0 43.30956 1 CGACTCC 55 8.5337844E-4 43.268356 6 TAGGACC 4470 0.0 40.124195 4 ATCAACG 6535 0.0 36.654545 3 TATCAAC 6595 0.0 36.59212 2 TCAACGC 6565 0.0 36.57781 4 CAACGCA 6640 0.0 35.98518 5 TACATGG 6480 0.0 34.850708 2 GTACATG 6590 0.0 34.821903 1 AACGCAG 6910 0.0 34.525455 6 ACATGGG 6275 0.0 33.330418 3 ACGCAGA 7905 0.0 30.104485 7 CGCAGAG 7975 0.0 29.691042 8 CATGGGG 3190 0.0 29.513027 4 GTAGGAC 15205 0.0 28.686136 3 TACAGTG 14695 0.0 28.50579 5 TGTAGGA 15850 0.0 28.383442 2 TTCGACT 105 5.8568455E-4 28.37449 4 >>END_MODULE