FastQCFastQC Report
Fri 27 May 2016
SRR939917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723785
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA33620.19503592385361285No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC27920.16196915508604612No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA19710.11434140568574386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAT109.0382586E-43007.1975110-111
CGTCGCT451.8189894E-122673.0645110-111
CCGCTCG850.02040.2168108-109
CCGCTCC4800.01806.4419108-109
CGTCGCG457.346898E-91651.604108-109
CCGCGCC3500.01628.0098108-109
CCGTTCG702.3646862E-111415.6606108-109
CGTGGCG1150.01292.5597108-109
CGTCGTC701.97615E-81288.799110-111
CGCGTCA200.00532713071238.703108-109
GTCCGCG501.2459383E-51202.879110-111
CTCCGCC3600.01169.4657110-111
CAGCGCG653.1957825E-81143.4182108-109
CCGCGCT704.297908E-81061.7455108-109
CGGCGCC1650.01051.0208108-109
CGTCGCC1700.01020.1084108-109
ACGCGCG250.008322525990.9624108-109
CCGTTCC1550.0958.9959108-109
CCGCGCG1551.8189894E-12799.16327108-109
TTGTTCG3100.0799.16327108-109
Produced by FastQC (version 0.11.5)