##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939917_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1723785 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.005132310583974 34.0 31.0 34.0 30.0 34.0 2 32.02166221425526 34.0 31.0 34.0 30.0 34.0 3 32.209430990523764 34.0 31.0 34.0 30.0 34.0 4 35.496651844632595 37.0 35.0 37.0 35.0 37.0 5 35.56058963269781 37.0 37.0 37.0 35.0 37.0 6 35.61002445200533 37.0 37.0 37.0 35.0 37.0 7 35.57236546321032 37.0 37.0 37.0 35.0 37.0 8 35.60084465290045 37.0 37.0 37.0 35.0 37.0 9 37.28820357527186 39.0 39.0 39.0 35.0 39.0 10-11 37.2624961929707 39.0 39.0 39.0 35.0 39.0 12-13 37.21016048985227 39.0 38.5 39.0 35.0 39.0 14-15 38.74780932656915 41.0 40.0 41.0 36.0 41.0 16-17 38.73281238669556 41.0 40.0 41.0 36.0 41.0 18-19 38.7370719086197 41.0 39.5 41.0 35.0 41.0 20-21 38.784393355319835 41.0 40.0 41.0 35.0 41.0 22-23 38.75649254402376 41.0 39.0 41.0 35.0 41.0 24-25 38.77784729534136 41.0 39.0 41.0 35.0 41.0 26-27 38.74768228056283 41.0 39.0 41.0 35.0 41.0 28-29 38.69084717641701 41.0 39.0 41.0 35.0 41.0 30-31 38.61224804717526 41.0 39.0 41.0 34.0 41.0 32-33 38.529067140043566 41.0 39.0 41.0 34.0 41.0 34-35 38.48016835046134 40.5 39.0 41.0 34.0 41.0 36-37 38.64198609455356 41.0 39.0 41.0 35.0 41.0 38-39 38.60475291292127 41.0 39.0 41.0 34.0 41.0 40-41 38.534841352024756 41.0 39.0 41.0 34.0 41.0 42-43 38.472408391997845 41.0 39.0 41.0 34.0 41.0 44-45 38.38287402431278 41.0 39.0 41.0 34.0 41.0 46-47 38.29238101039283 41.0 39.0 41.0 33.0 41.0 48-49 38.213472677857155 40.5 38.5 41.0 33.0 41.0 50-51 38.11060689123063 40.0 38.0 41.0 33.0 41.0 52-53 38.01898815687571 40.0 38.0 41.0 33.0 41.0 54-55 37.89358823751222 40.0 38.0 41.0 33.0 41.0 56-57 37.72000829569813 40.0 38.0 41.0 33.0 41.0 58-59 37.57557351989952 40.0 37.5 41.0 32.0 41.0 60-61 37.37964769388294 40.0 37.0 41.0 32.0 41.0 62-63 37.14129372282506 40.0 37.0 41.0 31.5 41.0 64-65 36.86897031822414 39.5 36.0 41.0 31.0 41.0 66-67 36.56965398817138 39.0 36.0 41.0 31.0 41.0 68-69 36.189007039741035 39.0 35.0 41.0 31.0 41.0 70-71 35.78138543959949 38.0 35.0 40.0 30.0 41.0 72-73 35.3816923224184 37.0 35.0 40.0 30.0 41.0 74-75 34.947927670794215 37.0 35.0 39.0 30.0 41.0 76-77 34.472698451373 36.0 34.5 39.0 29.0 40.5 78-79 34.01180657680627 36.0 34.0 38.5 29.0 39.5 80-81 33.574620384792766 35.5 34.0 37.0 29.0 39.0 82-83 33.35810469403087 35.0 34.0 37.0 29.0 39.0 84-85 32.93144997781046 35.0 34.0 36.5 29.0 38.5 86-87 32.292911238930614 35.0 34.0 36.0 27.5 37.0 88-89 16.569491844980668 18.0 17.0 18.5 2.0 19.5 90-91 2.7955055299819875 2.0 2.0 2.0 2.0 2.0 92-93 2.3070617855475017 2.0 2.0 2.0 2.0 2.0 94-95 2.1046264470337075 2.0 2.0 2.0 2.0 2.0 96-97 2.000033646887518 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14256660357660422 1101 2 0.13475666879066495 1101 3 0.1677451323559822 1101 4 0.23135616401633285 1101 5 0.2576305623643904 1101 6 0.12321675769599238 1101 7 0.20139761719921978 1101 8 0.23128795690166726 1101 9 0.20762505909659978 1101 10-11 0.21034820467215098 1101 12-13 0.21371932475697974 1101 14-15 0.21837307990917054 1101 16-17 0.2913278038168272 1101 18-19 0.22571017976915186 1101 20-21 0.22362799464750083 1101 22-23 0.26976629059780066 1101 24-25 0.17477975073265384 1101 26-27 0.23671318063740188 1101 28-29 0.31162802044119076 1101 30-31 0.2161499883876843 1101 32-33 0.28152826562088507 1101 34-35 0.2403164418854331 1101 36-37 0.22881252413288422 1101 38-39 0.24038602299474832 1101 40-41 0.1717876213672298 1101 42-43 0.19844939858066368 1101 44-45 0.19133146547213897 1101 46-47 0.20444248809182852 1101 48-49 0.14266432207212887 1101 50-51 0.12535799397213765 1101 52-53 0.047788215094435316 1101 54-55 0.05903352404008899 1101 56-57 0.09034784488809322 1101 58-59 0.07861142038292712 1101 60-61 0.03470044742013556 1101 62-63 0.11820258394329386 1101 64-65 0.2108071444789701 1101 66-67 0.13381325276729683 1101 68-69 0.20395138901152876 1101 70-71 0.2433030151016382 1101 72-73 0.2723967090352275 1101 74-75 0.21004207143299425 1101 76-77 0.21848184535022597 1101 78-79 0.37108468613609347 1101 80-81 0.2988051929384383 1101 82-83 0.2546110617299604 1101 84-85 0.30632135422614226 1101 86-87 0.4554596862018272 1101 88-89 0.7867312062598053 1101 90-91 -0.14125555248925004 1101 92-93 -0.20539941983068122 1101 94-95 -0.02089152454976073 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -5.6230500121046134E-5 1102 2 0.02558267237564138 1102 3 0.15888221601932884 1102 4 0.12849165667268636 1102 5 0.10001995611430914 1102 6 0.08775553935981151 1102 7 0.11346043765859548 1102 8 0.04669762383234399 1102 9 0.1826648342713355 1102 10-11 0.16747273744410762 1102 12-13 0.16832516840754153 1102 14-15 0.1546377915131032 1102 16-17 0.23622008083268042 1102 18-19 0.0834342929043288 1102 20-21 0.1955271810801733 1102 22-23 0.1402829857497423 1102 24-25 0.13044745259600177 1102 26-27 0.08382444343840945 1102 28-29 0.12308248915601894 1102 30-31 0.17938305006872923 1102 32-33 0.23631874250612128 1102 34-35 0.2355457657421951 1102 36-37 0.13949476390944682 1102 38-39 0.15635488719639312 1102 40-41 0.06749563684654447 1102 42-43 0.061945949082982565 1102 44-45 -0.010234616898948445 1102 46-47 0.07217665791965544 1102 48-49 0.07511261434497385 1102 50-51 0.03361950619301979 1102 52-53 0.05977365633834353 1102 54-55 0.08775866889814665 1102 56-57 0.08202336978144587 1102 58-59 0.10229718955491762 1102 60-61 0.08457104783421698 1102 62-63 0.1132103652875216 1102 64-65 0.08995383671291535 1102 66-67 0.10700217963655234 1102 68-69 0.1605201983269069 1102 70-71 0.21812958491835133 1102 72-73 0.16564769559851555 1102 74-75 0.19646075985964018 1102 76-77 0.13705546242472622 1102 78-79 0.19267756898517518 1102 80-81 0.2685173526783231 1102 82-83 0.2451441359167319 1102 84-85 0.2682809155528645 1102 86-87 0.4024074191905598 1102 88-89 1.137594970207168 1102 90-91 0.038156104937070445 1102 92-93 -0.042004391553831955 1102 94-95 0.07460050652003503 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.07016236752190963 1103 2 0.06927283817488927 1103 3 0.046229327367569795 1103 4 0.04831525378823187 1103 5 0.026897990817722928 1103 6 0.0011162877885624312 1103 7 -0.010932286099922806 1103 8 0.07372280075534121 1103 9 0.08590666091532029 1103 10-11 0.05702152926722448 1103 12-13 0.09578187302582819 1103 14-15 -0.029631581093944703 1103 16-17 0.08728178660864216 1103 18-19 0.06766675412221446 1103 20-21 0.06636298915437067 1103 22-23 0.05829842813274411 1103 24-25 0.06710146872292455 1103 26-27 0.12552860872090577 1103 28-29 0.15277082280813659 1103 30-31 0.21031999534926626 1103 32-33 0.22268208144327417 1103 34-35 0.22566072794158032 1103 36-37 0.11562173573263834 1103 38-39 0.19286738170162465 1103 40-41 0.15547988910568478 1103 42-43 0.2063624303538063 1103 44-45 0.17808846513494103 1103 46-47 0.1727210170204998 1103 48-49 -0.027991549595284937 1103 50-51 -0.07964762153045513 1103 52-53 -0.05307226210091187 1103 54-55 -0.0011930812050309214 1103 56-57 -0.009683563855240607 1103 58-59 -0.03214960938875322 1103 60-61 -0.01685952016495662 1103 62-63 -0.1395707401079136 1103 64-65 -0.17341152563948725 1103 66-67 -0.16452018027634097 1103 68-69 -0.23665549317441048 1103 70-71 -0.1631013092432667 1103 72-73 -0.17710013921294632 1103 74-75 -0.1448437557454909 1103 76-77 -0.1054667095756372 1103 78-79 -0.12262404739811217 1103 80-81 -0.17279504854176508 1103 82-83 -0.20864716708885567 1103 84-85 -0.08252534663209588 1103 86-87 0.0027203271033542364 1103 88-89 0.3614452643238302 1103 90-91 -0.6775792202690458 1103 92-93 -0.33123834600517776 1103 94-95 -0.11401232991888843 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.04368997610608005 1104 2 0.04366897871833686 1104 3 0.05681494219051331 1104 4 0.07719466440752143 1104 5 0.012153512084708495 1104 6 0.06303461558017176 1104 7 0.027139810728378677 1104 8 0.07316434402385141 1104 9 0.11562825513255603 1104 10-11 0.11389880502660787 1104 12-13 0.11283279733919471 1104 14-15 0.12610683494538932 1104 16-17 0.12455347163133013 1104 18-19 0.16426016806011035 1104 20-21 0.11720761479556785 1104 22-23 0.04311052575791763 1104 24-25 0.0915620663551735 1104 26-27 0.18415339134691777 1104 28-29 0.15251762148223236 1104 30-31 0.12601648536195853 1104 32-33 0.2289525094563416 1104 34-35 0.2339604617588691 1104 36-37 0.24730233061436735 1104 38-39 0.1839017091179329 1104 40-41 0.20337558686813395 1104 42-43 0.1346303019331785 1104 44-45 0.1643057015902798 1104 46-47 0.15005998033458923 1104 48-49 0.1787153615156285 1104 50-51 0.16159797475866355 1104 52-53 0.17947949957623877 1104 54-55 0.22373548903006224 1104 56-57 0.17223628700018168 1104 58-59 0.11128845975191837 1104 60-61 0.1501346266429806 1104 62-63 0.19072878292248419 1104 64-65 0.0866061534683169 1104 66-67 0.12170271143703815 1104 68-69 0.10884237517889517 1104 70-71 0.13792646964127897 1104 72-73 0.22775416077535482 1104 74-75 0.21278255251490918 1104 76-77 0.1552067866719682 1104 78-79 0.24737667236200167 1104 80-81 0.19528995255715387 1104 82-83 0.18961474076385798 1104 84-85 0.298490434108416 1104 86-87 0.21561894246483604 1104 88-89 0.8464363380131275 1104 90-91 -0.6395668892359456 1104 92-93 -0.33123834600517776 1104 94-95 -0.11401232991888843 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.067399443033338 1105 2 -0.14651347864895925 1105 3 -0.02847045185370689 1105 4 -0.04416040312418801 1105 5 -0.03606031646031482 1105 6 -0.08462753232144138 1105 7 -0.08231400357973229 1105 8 -0.16901128211963368 1105 9 -0.06910233613670869 1105 10-11 -0.10724645526550347 1105 12-13 -0.10402000040287618 1105 14-15 -0.19149652321929267 1105 16-17 -0.09841417684162934 1105 18-19 -0.03253871047024148 1105 20-21 0.02074889249668388 1105 22-23 -0.21410518855455507 1105 24-25 -0.08909820202325136 1105 26-27 0.014623990234191808 1105 28-29 -0.11088459648001958 1105 30-31 -0.08868969083866318 1105 32-33 -0.11107696346541474 1105 34-35 -0.1015403593617279 1105 36-37 -0.27015335072839974 1105 38-39 -0.29229609664802325 1105 40-41 -0.1831458590534183 1105 42-43 -0.27554488086762774 1105 44-45 -0.2877961173635555 1105 46-47 -0.2007178987390148 1105 48-49 -0.2774325838096061 1105 50-51 -0.2789670998290603 1105 52-53 -0.19408510044749505 1105 54-55 -0.26561835462608485 1105 56-57 -0.13321276917945113 1105 58-59 -0.24802844908808197 1105 60-61 -0.2533901037609638 1105 62-63 -0.18103247392279087 1105 64-65 -0.21316764810148214 1105 66-67 -0.1584943887271919 1105 68-69 -0.11508298714447562 1105 70-71 -0.19174064869374519 1105 72-73 -0.1489281998058587 1105 74-75 -0.24592850669942834 1105 76-77 -0.17165119380847216 1105 78-79 -0.20201154596241366 1105 80-81 -0.26383947228377735 1105 82-83 -0.2748983316009088 1105 84-85 -0.33171337863862504 1105 86-87 -0.21477032489578107 1105 88-89 0.6809452579429411 1105 90-91 -0.8096699820194511 1105 92-93 -0.33123834600517776 1105 94-95 -0.11401232991888843 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.1212001228396673 1106 2 -0.08645148911504208 1106 3 -0.05959604485023817 1106 4 -0.05390999964201626 1106 5 0.013555152801679071 1106 6 -0.01428897409774521 1106 7 -0.13087186047805233 1106 8 -0.020538401012380803 1106 9 0.0016348135673993625 1106 10-11 -0.09014889816665317 1106 12-13 -0.013688289149392574 1106 14-15 -0.00884564533989618 1106 16-17 -0.02684927734537723 1106 18-19 -0.062489620139146496 1106 20-21 -0.034001804427077786 1106 22-23 -0.0333583185980828 1106 24-25 -0.029995093987842836 1106 26-27 -0.20125578430485547 1106 28-29 0.007670998472789847 1106 30-31 -0.06577740541781907 1106 32-33 0.05231253132599534 1106 34-35 -0.06146510382301784 1106 36-37 0.0926003539715552 1106 38-39 0.08486161597485875 1106 40-41 0.14134398053185038 1106 42-43 -0.2137916285567698 1106 44-45 -0.0569737473337284 1106 46-47 -0.03236526754617586 1106 48-49 0.05299822511101837 1106 50-51 0.011712156296539433 1106 52-53 -0.06297479086662605 1106 54-55 0.05317015958138427 1106 56-57 -0.06942590558087858 1106 58-59 0.050925553944146884 1106 60-61 0.08128429835181805 1106 62-63 0.1704179222233435 1106 64-65 0.24139017211663827 1106 66-67 0.2715078732183329 1106 68-69 0.2733178173745259 1106 70-71 0.23980401556017483 1106 72-73 0.2454872401119772 1106 74-75 0.06686707455943264 1106 76-77 0.09264552453652186 1106 78-79 0.13699692716323142 1106 80-81 0.1519800855975717 1106 82-83 0.23829889782149394 1106 84-85 0.22797294453836514 1106 86-87 0.2827516220891084 1106 88-89 0.5583765437061317 1106 90-91 -0.33703799444181115 1106 92-93 -0.3091110168126314 1106 94-95 -0.11401232991888843 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.09352006230837873 1107 2 -0.10276394591631899 1107 3 -0.045167671575526924 1107 4 -0.0998498505076384 1107 5 -0.08489267139810153 1107 6 -0.11181591358768372 1107 7 -0.09815892763570133 1107 8 -0.12082637283678821 1107 9 -0.11015861456525755 1107 10-11 -0.07632980380059706 1107 12-13 -0.12465460181260113 1107 14-15 -0.11236408544435506 1107 16-17 -0.11341966442951446 1107 18-19 -0.04878316681027428 1107 20-21 -0.11245378042214327 1107 22-23 -0.013613922342244678 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0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14780.0 3 2609.0 4 1884.0 5 1666.0 6 1697.0 7 1876.0 8 2155.0 9 2512.0 10 2972.0 11 3577.0 12 4450.0 13 5161.0 14 6013.0 15 6899.0 16 7609.0 17 8652.0 18 10800.0 19 13440.0 20 14804.0 21 21128.0 22 24907.0 23 32672.0 24 49455.0 25 82852.0 26 158929.0 27 484561.0 28 729088.0 29 25725.0 30 912.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29604330006352 20.010268101880456 14.286526451964718 24.407162146091306 2 13.074063502892507 20.049322297019778 44.681638553769545 22.194975646318174 3 18.832706052366596 25.237820254259173 29.315078828469364 26.61439486490486 4 11.497615158975858 17.12517832621189 40.02441139291199 31.352795121900257 5 12.474164514797835 38.80508740679346 36.02347408848113 12.69727398992757 6 30.164492671649885 37.58862039059395 19.47354223409532 12.773344703660838 7 27.781596892883975 31.925907233210637 22.601194464506886 17.691301409398505 8 23.61634426567118 39.31720023088726 21.02611404554512 16.04034145789643 9 25.568791931708425 17.531942788688845 21.71871782153807 35.180547458064666 10-11 23.78299560005488 26.91093338067139 30.12977648139559 19.176294537878135 12-13 25.671259252684468 24.416180591632287 29.257380610312417 20.65517954537082 14-15 20.445479118764556 28.87086523866527 26.152456992158825 24.531198650411348 16-17 20.308195317607787 30.173276473697 29.307967329880835 20.21056087881437 18-19 21.140658667418556 28.534219585974512 30.09775118323662 20.22737056337031 20-21 22.10596755685556 28.416931882391573 28.500209426635127 20.976891134117746 22-23 21.006737826358478 30.11903232178436 28.475836905675944 20.398392946181218 24-25 21.511499403927985 28.563306908924257 29.708374304220076 20.216819382927685 26-27 20.096328718488675 29.89441838744391 29.251675818039953 20.757577076027463 28-29 20.356005294220534 29.672667382734264 28.08628662872843 21.885040694316775 30-31 21.209750635955178 28.609919450511516 28.974205019767545 21.206124893765754 32-33 20.250596949466402 29.69008518567507 29.431581121313588 20.62773674354494 34-35 20.573088209743275 29.874198238931974 29.357340988587023 20.19537256273772 36-37 20.158378495656642 29.12296914575045 29.275743684526233 21.442908674066675 38-39 21.17152081030987 29.710259690158576 28.506832348581757 20.6113871509498 40-41 22.427303869102005 28.159428234959694 29.312385245259705 20.100882650678596 42-43 20.34705604237187 29.84722572710634 29.466290749716467 20.339427480805323 44-45 20.159045633380554 29.878488115086476 28.92799089444025 21.03447535709272 46-47 21.361045606035557 28.529050896718562 28.84852229251328 21.26138120473261 48-49 20.94571703382446 28.881291253766427 28.702991445554023 21.470000266855088 50-51 20.27114201729568 30.117828056083624 28.688297772979542 20.92273215364115 52-53 20.287158781402553 29.534744762252835 29.228384050215077 20.949712406129535 54-55 20.361089388521343 28.752416235244432 29.716212735445158 21.170281640789067 56-57 20.837024338882166 29.015219415414332 28.190290552476093 21.957465693227405 58-59 21.237364288469852 29.324248673703508 27.72248279222757 21.715904245599074 60-61 20.686483523177195 28.21773016936567 29.73555286767201 21.360233439785123 62-63 21.013960557726165 28.327372613173917 29.459416342525312 21.199250486574602 64-65 21.220843214695112 28.998963907310895 28.345923851248184 21.43426902674581 66-67 21.18764811156844 29.897347987132967 27.712852820972454 21.202151080326143 68-69 21.71825109526992 28.333756822232125 29.29783220093562 20.650159881562338 70-71 22.188207926162484 28.362788862880233 28.64437850427983 20.80462470667746 72-73 21.512369582053445 29.221335607398835 28.274755842520964 20.991538968026756 74-75 20.736634789141338 29.729229573293654 29.177130558625354 20.357005078939657 76-77 21.196233869073 28.39919131446207 29.506521985050338 20.89805283141459 78-79 20.885729161681247 28.50185131201678 28.96104630673042 21.65137321957155 80-81 21.327572754142775 29.16523812424403 28.73612428464107 20.771064836972126 82-83 20.87494876780219 29.25417232408393 29.460406394242383 20.41047251387149 84-85 20.94532172978799 28.701313128939915 29.516786765855457 20.836578375416632 86-87 20.12747126776856 29.942579046631174 28.284228498034242 21.645721187566025 88-89 29.986410818482433 18.096307937173737 35.13338514268641 16.78389610165742 90-91 31.12306791509227 15.239852413034304 17.221494091920587 36.41558557995283 92-93 22.92004276677086 17.58664330928067 36.04952893831345 23.443784985635013 94-95 15.757190846105395 7.039838337182449 28.31445517530968 48.88851564140248 96-97 26.97096580096351 7.227845543442103 15.650369323026926 50.150819332567465 98-99 57.42156174819567 14.018227078321305 18.768795108259823 9.791416065223203 100-101 30.97454653479398 7.073173225121879 12.755717813937798 49.19656242614634 102-103 23.070468810903495 2.011066349139659 37.81304203142922 37.10542280852762 104-105 41.067329564142604 4.509987893811764 41.82047526886932 12.60220727317631 106-107 5.860826679043804 1.7523751787896298 7.00034416017025 85.38645398199631 108-109 18.681224231901602 11.231605593120896 22.063200036105204 48.02397013887229 110-111 40.516803285117945 2.738089522126199 25.482244989058955 31.2628622036969 112-113 27.329136919205915 2.7378152908100466 39.447644267368304 30.485403522615734 114-115 28.547678657706328 3.8683181798216717 24.51333069794 43.070672464532 116-117 19.81892148536618 4.194780912120965 20.925041555175415 55.06125604733744 118-119 20.46069731635846 4.285072761286691 16.07763687234078 59.17659305001407 120-121 25.555140386463115 4.209518047993547 30.377032845806202 39.85830871973714 122-123 34.22051309218225 1.606362843919612 34.61879283192788 29.554331231970256 124-125 38.681717055861114 1.9353822310145992 20.043576910980672 39.33932380214362 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10.0 1 26.0 2 63.0 3 105.5 4 139.0 5 135.0 6 119.5 7 108.0 8 96.5 9 87.0 10 94.5 11 101.5 12 95.0 13 119.0 14 150.0 15 173.0 16 231.0 17 368.5 18 547.5 19 835.0 20 1275.0 21 1923.5 22 2861.0 23 4079.0 24 5878.5 25 8283.5 26 11219.0 27 15023.0 28 19647.0 29 25070.5 30 31780.0 31 39642.5 32 48670.5 33 58739.0 34 68310.0 35 75551.5 36 79286.5 37 80050.0 38 79381.5 39 78166.5 40 76791.0 41 74465.0 42 71714.5 43 69601.0 44 67430.5 45 65278.0 46 62907.0 47 60062.0 48 56744.5 49 53279.0 50 49574.0 51 45065.0 52 40241.0 53 35125.5 54 30088.0 55 25686.5 56 21577.0 57 17844.0 58 14454.0 59 11468.0 60 9136.0 61 7088.5 62 5239.5 63 3920.0 64 3005.5 65 2144.5 66 1488.0 67 1077.0 68 773.0 69 567.5 70 405.5 71 301.0 72 231.5 73 159.5 74 113.5 75 78.0 76 47.0 77 32.5 78 27.5 79 17.0 80 13.5 81 6.5 82 5.5 83 7.0 84 7.0 85 8.0 86 5.0 87 1.0 88 4.0 89 8.0 90 7.0 91 5.0 92 4.0 93 2.5 94 2.0 95 2.0 96 2.5 97 4.0 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.001218249375647195 3 0.04292878752280592 4 0.04751172565024061 5 0.050122260026627445 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.831603129160538E-4 12-13 0.001653338438378336 14-15 2.9005937515409405E-5 16-17 4.640950002465505E-4 18-19 0.007135460628790714 20-21 0.001798368125955383 22-23 5.511128127927787E-4 24-25 0.0 26-27 0.0 28-29 0.001073219688070148 30-31 0.0 32-33 0.001218249375647195 34-35 8.701781254622822E-5 36-37 1.7403562509245643E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.7403562509245643E-4 46-47 0.0 48-49 1.7403562509245643E-4 50-51 1.1602375006163762E-4 52-53 0.0 54-55 0.0016823443758937455 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.801187503081881E-5 66-67 0.0 68-69 5.221068752773693E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.350890627311411E-4 80-81 0.0 82-83 8.991840629776915E-4 84-85 8.701781254622822E-5 86-87 0.0013922850007396514 88-89 19.230994584591464 90-91 26.236653643000725 92-93 40.34241509236941 94-95 44.73788784564201 96-97 48.4672102379357 98-99 93.05528241631062 100-101 51.765533404687936 102-103 53.19250950669602 104-105 74.86638414883527 106-107 81.62688502336428 108-109 12.593043796065054 110-111 38.984647157273095 112-113 52.06705012516062 114-115 86.79243641173349 116-117 66.46060848655719 118-119 71.96726970010762 120-121 80.43769959710752 122-123 75.90848046595138 124-125 50.1046534225556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1723785.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.08202168897299 #Duplication Level Percentage of deduplicated Percentage of total 1 79.24860960729885 53.95405558105073 2 13.01209832976614 17.717799214123744 3 3.939523112759735 8.046320940213558 4 1.5769070570445793 4.294360818368144 5 0.7804746967193916 2.6568147614872117 6 0.42423947711694643 1.73298487694367 7 0.25025920341689983 1.1926706760426133 8 0.16958692937940958 0.9236656803340234 9 0.1151053082534878 0.705294188372689 >10 0.4500307859264363 4.993838810653106 >50 0.019102831077978524 0.9212638034397014 >100 0.01218806246563431 1.5681324750563366 >500 0.0013633445633117454 0.5968278040490433 >1k 5.112542112419046E-4 0.6959703698655105 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3362 0.19503592385361285 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2792 0.16196915508604612 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1971 0.11434140568574386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.9005937515409405E-5 0.0 18-19 2.9005937515409405E-5 0.0 0.0 5.801187503081881E-5 0.0 20-21 5.801187503081881E-5 0.0 0.0 8.701781254622822E-5 0.0 22-23 5.801187503081881E-5 0.0 0.0 2.3204750012327524E-4 0.0 24-25 5.801187503081881E-5 0.0 0.0 2.9005937515409405E-4 0.0 26-27 5.801187503081881E-5 0.0 0.0 4.0608312521573167E-4 0.0 28-29 5.801187503081881E-5 0.0 0.0 0.0012472553131626044 0.0 30-31 5.801187503081881E-5 0.0 0.0 0.004495920314888458 0.0 32-33 5.801187503081881E-5 0.0 0.0 0.010645179068155252 0.0 34-35 5.801187503081881E-5 0.0 0.0 0.018389764384769563 0.0 36-37 5.801187503081881E-5 0.0 0.0 0.03286372720495886 0.0 38-39 5.801187503081881E-5 0.0 0.0 0.05632953065492506 0.0 40-41 5.801187503081881E-5 0.0 0.0 0.08252189223133975 0.0 42-43 5.801187503081881E-5 0.0 0.0 0.1103675922461328 0.0 44-45 5.801187503081881E-5 0.0 0.0 0.1409108444498589 0.0 46-47 5.801187503081881E-5 0.0 0.0 0.1769942307190282 0.0 48-49 5.801187503081881E-5 0.0 0.0 0.21644230573998496 0.0 50-51 5.801187503081881E-5 0.0 0.0 0.2602412713882532 0.0 52-53 5.801187503081881E-5 0.0 0.0 0.30633170610023874 0.0 54-55 5.801187503081881E-5 0.0 0.0 0.35726613237729765 0.0 56-57 5.801187503081881E-5 0.0 0.0 0.41690233990897935 0.0 58-59 5.801187503081881E-5 0.0 0.0 0.4861395127582616 0.0 60-61 5.801187503081881E-5 0.0 0.0 0.5634403362368277 0.0 62-63 5.801187503081881E-5 0.0 0.0 0.6472965015938763 0.0 64-65 5.801187503081881E-5 0.0 0.0 0.7299634235127931 0.0 66-67 5.801187503081881E-5 0.0 0.0 0.8282065338774847 0.0 68-69 5.801187503081881E-5 0.0 0.0 0.9436211592512987 0.0 70-71 5.801187503081881E-5 0.0 0.0 1.0688687974428366 0.0 72-73 5.801187503081881E-5 0.0 0.0 1.1964369106356072 0.0 74-75 5.801187503081881E-5 0.0 0.0 1.3209884063267752 0.0 76-77 5.801187503081881E-5 0.0 0.0 1.4625373814019729 0.0 78-79 1.1602375006163762E-4 0.0 0.0 1.5036678007988233 0.0 80-81 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 82-83 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 84-85 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 86-87 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 88-89 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 90-91 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 92-93 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 94-95 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 96-97 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 98-99 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 100-101 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 102-103 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 104-105 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 106-107 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 108-109 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 110-111 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 112-113 1.1602375006163762E-4 0.0 0.0 1.5043639432991933 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGAT 10 9.0382586E-4 3007.1975 110-111 CGTCGCT 45 1.8189894E-12 2673.0645 110-111 CCGCTCG 85 0.0 2040.2168 108-109 CCGCTCC 480 0.0 1806.4419 108-109 CGTCGCG 45 7.346898E-9 1651.604 108-109 CCGCGCC 350 0.0 1628.0098 108-109 CCGTTCG 70 2.3646862E-11 1415.6606 108-109 CGTGGCG 115 0.0 1292.5597 108-109 CGTCGTC 70 1.97615E-8 1288.799 110-111 CGCGTCA 20 0.0053271307 1238.703 108-109 GTCCGCG 50 1.2459383E-5 1202.879 110-111 CTCCGCC 360 0.0 1169.4657 110-111 CAGCGCG 65 3.1957825E-8 1143.4182 108-109 CCGCGCT 70 4.297908E-8 1061.7455 108-109 CGGCGCC 165 0.0 1051.0208 108-109 CGTCGCC 170 0.0 1020.1084 108-109 ACGCGCG 25 0.008322525 990.9624 108-109 CCGTTCC 155 0.0 958.9959 108-109 CCGCGCG 155 1.8189894E-12 799.16327 108-109 TTGTTCG 310 0.0 799.16327 108-109 >>END_MODULE