FastQCFastQC Report
Fri 27 May 2016
SRR939917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723785
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA34080.19770447010503048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.1794887413453534No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC28320.16428963008727887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22400.12994660006903413No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA19100.11080268130886392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65900.037.9805031
ATCAACG74850.033.432283
GGTATCA46300.033.2074551
TCAACGC75950.032.948084
CAACGCA77100.032.533915
AACGCAG78350.031.9684316
TATCAAC79350.031.4612262
TAGGACC24150.031.3325064
GTACATG60400.029.7966121
ACGCAGA88900.028.1077297
TACATGG61400.028.0438752
CGCAGAG90000.027.7641898
GTCCTAC65600.027.6163711
ACATGGG55050.027.4906443
GTAGGAC69450.026.9380383
CTACAGT71350.026.8887394
TCCTACA74100.026.3732812
AGTGGAC46600.026.3002288
TACAGTG74500.026.0717325
CCTACAG73150.025.7383863
Produced by FastQC (version 0.11.5)