FastQCFastQC Report
Fri 27 May 2016
SRR939916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996524
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23310.23391308187258913No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19630.19698471888283672No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17860.17922297907526563No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA13250.132962176525603No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12710.12754334065210673No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA10950.10988194965700776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG52.1961442E-46096.268110-111
CGACGCC400.03156.3518108-109
AACGTCG100.00128067342525.0815108-109
CGTCGCG201.3440404E-62525.0815108-109
CCGCTCG451.8189894E-122244.5168108-109
CGCGTCG150.00197610072032.0895110-111
CGTTTCG150.00197610072032.0895110-111
CGGCGCG252.6246944E-62020.0653108-109
CCGCTCC2400.01999.0227108-109
CCGCGCC1750.01731.4843108-109
CCGCGCG750.01683.3877108-109
AACCGCG150.0028811371683.3876108-109
CCGTTCG304.5348042E-61683.3876108-109
CGCGGCG501.0293661E-81515.049108-109
CGTCGCC902.910383E-111354.7262110-111
CGGCGCA200.00512134941262.5408108-109
GCGCGTT200.00512134941262.5408108-109
CATCGCG401.0746002E-51262.5408108-109
GCTCGCG200.00512134941262.5408108-109
CGGCGCC1400.01262.5406108-109
Produced by FastQC (version 0.11.5)