##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939916_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 996524 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.892566561367314 34.0 31.0 34.0 30.0 34.0 2 31.960091277279826 34.0 31.0 34.0 30.0 34.0 3 32.04177019319153 34.0 31.0 34.0 30.0 34.0 4 35.25991446267225 37.0 35.0 37.0 33.0 37.0 5 35.27502297987806 37.0 35.0 37.0 33.0 37.0 6 35.363040930273634 37.0 37.0 37.0 33.0 37.0 7 35.271573991193385 37.0 37.0 37.0 33.0 37.0 8 35.30916064239296 37.0 37.0 37.0 33.0 37.0 9 36.967205004595975 39.0 38.0 39.0 34.0 39.0 10-11 36.94615132199526 39.0 38.0 39.0 34.0 39.0 12-13 36.91250035122084 39.0 38.0 39.0 34.0 39.0 14-15 38.44373492259093 41.0 39.0 41.0 34.0 41.0 16-17 38.465035463270326 41.0 39.0 41.0 34.0 41.0 18-19 38.47860462969281 41.0 39.5 41.0 34.0 41.0 20-21 38.51058378925144 41.0 39.5 41.0 34.0 41.0 22-23 38.50851961417889 41.0 39.0 41.0 34.0 41.0 24-25 38.53936483215658 41.0 39.0 41.0 34.0 41.0 26-27 38.50721056392018 41.0 39.0 41.0 34.0 41.0 28-29 38.44770572510045 41.0 39.0 41.0 34.0 41.0 30-31 38.36785867676042 41.0 39.0 41.0 34.0 41.0 32-33 38.27146210226748 41.0 39.0 41.0 33.0 41.0 34-35 38.22772707932774 40.0 38.5 41.0 33.0 41.0 36-37 38.393679931441696 41.0 39.0 41.0 34.0 41.0 38-39 38.348860137839125 41.0 39.0 41.0 33.0 41.0 40-41 38.25770528356567 41.0 39.0 41.0 33.0 41.0 42-43 38.21647998442586 41.0 39.0 41.0 33.0 41.0 44-45 38.16012459308557 41.0 39.0 41.0 33.0 41.0 46-47 38.05547432876679 41.0 38.0 41.0 33.0 41.0 48-49 37.97445269757678 41.0 38.0 41.0 33.0 41.0 50-51 37.872072323396125 40.5 38.0 41.0 33.0 41.0 52-53 37.78573521560946 40.0 38.0 41.0 32.5 41.0 54-55 37.65601581095889 40.0 38.0 41.0 32.0 41.0 56-57 37.46157292749598 40.0 38.0 41.0 31.5 41.0 58-59 37.31199248588092 40.0 37.0 41.0 31.0 41.0 60-61 37.107923140837556 40.0 37.0 41.0 31.0 41.0 62-63 36.86280611405245 40.0 36.5 41.0 31.0 41.0 64-65 36.57221652463964 39.5 36.0 41.0 30.5 41.0 66-67 36.25149419381771 39.0 35.5 41.0 30.0 41.0 68-69 35.83278927552171 39.0 35.0 41.0 29.5 41.0 70-71 35.39829848553572 38.0 35.0 40.0 29.0 41.0 72-73 34.99534682556566 37.0 35.0 40.0 29.0 41.0 74-75 34.5589228157074 37.0 34.5 39.0 28.0 41.0 76-77 34.07023814780176 36.0 34.0 39.0 28.0 40.5 78-79 33.58584941255805 36.0 34.0 38.5 26.5 40.0 80-81 33.127304510478425 35.0 34.0 37.0 26.0 39.0 82-83 32.88708500748602 35.0 34.0 37.0 26.0 39.0 84-85 32.41263080467706 35.0 34.0 36.5 26.0 39.0 86-87 31.74654448864252 35.0 34.0 36.0 13.0 37.0 88-89 16.209014032777937 18.0 16.5 18.5 2.0 19.5 90-91 2.7802867768362827 2.0 2.0 2.0 2.0 2.0 92-93 2.304165278508094 2.0 2.0 2.0 2.0 2.0 94-95 2.102590103198719 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3122557723622812 1101 2 0.2090052968230296 1101 3 0.2790304709590501 1101 4 0.25922378972871485 1101 5 0.28910145698380774 1101 6 0.24272615829156763 1101 7 0.27722750694113074 1101 8 0.3362898934586269 1101 9 0.5338959047198699 1101 10-11 0.3961495277295768 1101 12-13 0.4084167503564444 1101 14-15 0.5014293910665799 1101 16-17 0.639202299541509 1101 18-19 0.6024764361512638 1101 20-21 0.5645486524430652 1101 22-23 0.4627603243893219 1101 24-25 0.43746390908052746 1101 26-27 0.5192516614956375 1101 28-29 0.537695194528446 1101 30-31 0.5342112958070118 1101 32-33 0.5916318920368724 1101 34-35 0.5191753137973336 1101 36-37 0.5889040567002581 1101 38-39 0.5287176182061799 1101 40-41 0.5436659042140874 1101 42-43 0.5083681060631378 1101 44-45 0.5412876234670136 1101 46-47 0.5617401843501781 1101 48-49 0.5553261093802533 1101 50-51 0.4376682321588419 1101 52-53 0.3851683730445288 1101 54-55 0.3835684283214533 1101 56-57 0.3802856161474324 1101 58-59 0.37246558437036015 1101 60-61 0.42517727489806845 1101 62-63 0.4565213390366054 1101 64-65 0.43912148915220683 1101 66-67 0.42973178800530576 1101 68-69 0.42192271565953376 1101 70-71 0.36533301333023616 1101 72-73 0.3205547998308802 1101 74-75 0.16995722312945105 1101 76-77 0.3229928889922249 1101 78-79 0.3995838289637561 1101 80-81 0.44078236257792724 1101 82-83 0.5128117426322305 1101 84-85 0.5940145489369755 1101 86-87 0.7046159974147415 1101 88-89 0.66905185499078 1101 90-91 -0.19272032054819066 1101 92-93 -0.1549512753344482 1101 94-95 0.003762406102906901 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.005294191692353678 1102 2 0.06439461778244038 1102 3 0.07519229044797271 1102 4 0.08057089122534933 1102 5 0.18536496917255363 1102 6 0.19105162721517388 1102 7 0.046294626737221733 1102 8 0.1691050035821462 1102 9 0.15986754056066133 1102 10-11 0.05346186025685995 1102 12-13 0.18376372114266815 1102 14-15 0.21028894272986776 1102 16-17 0.2344313695369351 1102 18-19 0.21626769162476478 1102 20-21 0.2457124979433587 1102 22-23 0.22519075377302045 1102 24-25 0.17905439336260542 1102 26-27 0.23163804147079503 1102 28-29 0.11443841392587473 1102 30-31 0.13763407863167032 1102 32-33 0.15627158878871938 1102 34-35 0.07016100100732103 1102 36-37 0.008871117676669371 1102 38-39 0.03474545952373376 1102 40-41 0.0905383962080748 1102 42-43 0.04351299855382962 1102 44-45 -0.001083986231698475 1102 46-47 0.012907559031575033 1102 48-49 -0.022207646583417784 1102 50-51 -0.044040152914021746 1102 52-53 -0.0736800682338199 1102 54-55 -0.0334986914746338 1102 56-57 0.03195936296136637 1102 58-59 -0.06543860489831843 1102 60-61 -0.09226106246549648 1102 62-63 -0.0415632305993654 1102 64-65 -0.16584367652323095 1102 66-67 0.048274366921724265 1102 68-69 0.05301636966908063 1102 70-71 -0.03423982504634182 1102 72-73 -0.13821965935479597 1102 74-75 -0.08295827258978505 1102 76-77 -0.15117611956931398 1102 78-79 -0.14892175890631165 1102 80-81 -0.23094549834620182 1102 82-83 -0.2607905055868329 1102 84-85 -0.3904248994650885 1102 86-87 0.002644884415587967 1102 88-89 0.8034464697218446 1102 90-91 0.0328359176061781 1102 92-93 0.04492847108735454 1102 94-95 0.14186266098820832 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.03951743812673314 1103 2 0.028923601056000336 1103 3 0.06750492492464133 1103 4 -0.15830910119326091 1103 5 -0.029055362808733776 1103 6 -0.02816566568884582 1103 7 0.06123730012456008 1103 8 0.0983402768102124 1103 9 0.12736488223420395 1103 10-11 -0.022898471754992045 1103 12-13 0.15019008970313763 1103 14-15 0.13762937818081156 1103 16-17 0.15083814774716586 1103 18-19 0.2599095912314766 1103 20-21 0.24500403547251182 1103 22-23 0.13681534661726857 1103 24-25 0.1685892231711179 1103 26-27 0.14192442742567124 1103 28-29 0.156006385817669 1103 30-31 1.485421184668212E-4 1103 32-33 0.029747477372865205 1103 34-35 -0.02391649729730716 1103 36-37 0.01751278036497439 1103 38-39 0.012144716975583947 1103 40-41 0.021748244269502948 1103 42-43 0.047875354307457485 1103 44-45 0.07646654235110617 1103 46-47 0.05220162369042214 1103 48-49 0.07594179135950441 1103 50-51 -0.02618847810467173 1103 52-53 0.06636546086103579 1103 54-55 -0.034975737647236826 1103 56-57 -0.09990509321407615 1103 58-59 -0.17087646833243753 1103 60-61 -0.07859851274447038 1103 62-63 -0.016994027241338472 1103 64-65 -0.1060696712111664 1103 66-67 -0.18835209834221445 1103 68-69 -0.2527474731169548 1103 70-71 -0.15296116874566223 1103 72-73 -0.16897345221473614 1103 74-75 -0.18984949035550613 1103 76-77 -0.17623138779406844 1103 78-79 -0.11853124094152889 1103 80-81 -0.12421783070858794 1103 82-83 -0.060846397951493714 1103 84-85 -0.16496799084782765 1103 86-87 -0.2747384256250136 1103 88-89 0.533152879409311 1103 90-91 -0.5332166803188239 1103 92-93 -0.34040582078899373 1103 94-95 -0.11514668480618395 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.15786566247217237 1104 2 0.16850779432552798 1104 3 0.18078821321679328 1104 4 0.20629621730113712 1104 5 0.15855999844234958 1104 6 0.19189249195790126 1104 7 0.15997825619187722 1104 8 0.13475792542665488 1104 9 0.3423544461784118 1104 10-11 0.3451552719853197 1104 12-13 0.33403138572108304 1104 14-15 0.4170550154422017 1104 16-17 0.4432482535874698 1104 18-19 0.3942421704170016 1104 20-21 0.40445299809741186 1104 22-23 0.2754019327809303 1104 24-25 0.30337399899061523 1104 26-27 0.4214359771799465 1104 28-29 0.43998465506791007 1104 30-31 0.32601024685596514 1104 32-33 0.3997757481807298 1104 34-35 0.3827187703407873 1104 36-37 0.40027118956739827 1104 38-39 0.48305153429009806 1104 40-41 0.431829990128179 1104 42-43 0.3113733508183927 1104 44-45 0.35863602506541525 1104 46-47 0.3884692053292014 1104 48-49 0.3535401453442901 1104 50-51 0.2737746257652418 1104 52-53 0.2927957456719028 1104 54-55 0.3758259208289445 1104 56-57 0.30165549626731547 1104 58-59 0.2633651847699525 1104 60-61 0.29340379837459807 1104 62-63 0.2741429674082383 1104 64-65 0.32058053011125054 1104 66-67 0.3055821876057152 1104 68-69 0.326088799575615 1104 70-71 0.29848586048308334 1104 72-73 0.3400398148158956 1104 74-75 0.2851382920605232 1104 76-77 0.1558999818993101 1104 78-79 0.2183435692235065 1104 80-81 0.14099914579470862 1104 82-83 0.3892910133615075 1104 84-85 0.27922758590000996 1104 86-87 0.572606756655496 1104 88-89 0.8227638929528176 1104 90-91 -0.6218864044642745 1104 92-93 -0.34040582078899373 1104 94-95 -0.11514668480618395 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.020775155472769313 1105 2 -0.1186698047077428 1105 3 -0.11526131285850738 1105 4 0.012810793789945762 1105 5 -0.05556333526861579 1105 6 -0.026962064138920994 1105 7 -0.02114925032222459 1105 8 -0.09035677945608711 1105 9 0.0621658203712272 1105 10-11 0.04364593510227621 1105 12-13 0.038683541984056546 1105 14-15 0.006665254859143488 1105 16-17 0.08443816333407739 1105 18-19 0.03953360578574916 1105 20-21 0.09341088299601807 1105 22-23 0.02235045247429923 1105 24-25 0.05516962604398401 1105 26-27 0.1818958351913551 1105 28-29 0.0942237793456897 1105 30-31 0.08940404807193403 1105 32-33 0.019514428744152212 1105 34-35 0.08316531692135953 1105 36-37 -0.07407126351217386 1105 38-39 0.03931805556950252 1105 40-41 0.014111389999783341 1105 42-43 0.002823588724808701 1105 44-45 0.06967625202059224 1105 46-47 0.16886670731175002 1105 48-49 0.15996715905222914 1105 50-51 -0.014415491677453929 1105 52-53 0.04957324652156814 1105 54-55 0.11229570104872266 1105 56-57 0.04825187669101183 1105 58-59 -0.11112454371461666 1105 60-61 -0.14443347174360355 1105 62-63 -0.08793101960132077 1105 64-65 0.06315397899913222 1105 66-67 0.17472397794595196 1105 68-69 0.07295801712783145 1105 70-71 -0.006851928875320823 1105 72-73 0.05472912035571653 1105 74-75 0.11220652022411315 1105 76-77 0.06949085837678837 1105 78-79 0.07012928350921754 1105 80-81 0.013038532524817015 1105 82-83 0.1781700681555023 1105 84-85 0.11203766671267346 1105 86-87 0.1628915363088339 1105 88-89 0.8867250821073078 1105 90-91 -0.8021748660027361 1105 92-93 -0.34040582078899373 1105 94-95 -0.11514668480618395 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.03671515393927294 1106 2 -0.11925532153089691 1106 3 -0.04348670895366524 1106 4 -0.12501568411725117 1106 5 -0.10267234595429642 1106 6 0.014047812972250995 1106 7 -0.14398928573205438 1106 8 0.023509513595080023 1106 9 -0.09408073797482785 1106 10-11 -0.03178187555273837 1106 12-13 -0.0430371804321652 1106 14-15 0.06593489232664496 1106 16-17 -0.0059879395635391575 1106 18-19 0.03408132889205717 1106 20-21 -0.12392808245151343 1106 22-23 -0.09858874439179743 1106 24-25 -0.04168391807541383 1106 26-27 -0.056447152199119444 1106 28-29 0.047075734205130004 1106 30-31 0.08363461623236645 1106 32-33 0.17052967309287226 1106 34-35 -0.049629898569747866 1106 36-37 0.1804403527233731 1106 38-39 0.162930415550818 1106 40-41 0.23774814651540055 1106 42-43 -0.07394254609740614 1106 44-45 0.16359802976119653 1106 46-47 -0.0033512164734759153 1106 48-49 0.04550768169569608 1106 50-51 0.028529379125473042 1106 52-53 0.08822572137571427 1106 54-55 0.1422483717721832 1106 56-57 0.05091319006101003 1106 58-59 0.12867875051455258 1106 60-61 0.09680085348310996 1106 62-63 0.06643909673529436 1106 64-65 0.1385518167691444 1106 66-67 0.1021812317326507 1106 68-69 0.22840227801739843 1106 70-71 0.1873493634439498 1106 72-73 0.12092040312548846 1106 74-75 -0.09980981848342907 1106 76-77 0.0028785611294139812 1106 78-79 0.03409142008798227 1106 80-81 0.014022758422783 1106 82-83 0.15883964846540977 1106 84-85 0.4029670352606658 1106 86-87 0.3627486423222308 1106 88-89 0.8028442208399404 1106 90-91 -0.15782057256122162 1106 92-93 -0.2903720141628954 1106 94-95 -0.11514668480618395 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.07249503669920543 1107 2 -0.09099823362710424 1107 3 -0.07880183265966068 1107 4 -0.03810836678790963 1107 5 -0.09457844887085542 1107 6 -0.1410714292341737 1107 7 -0.1817004130353368 1107 8 -0.10522349283146326 1107 9 -0.04015293317362989 1107 10-11 0.0027291099596453705 1107 12-13 -0.03194335555706118 1107 14-15 0.004706825606149323 1107 16-17 0.019372937964575954 1107 18-19 0.02465354706623657 1107 20-21 -0.06745723164048911 1107 22-23 -0.009775127214091128 1107 24-25 -6.873118668053735E-4 1107 26-27 -0.07063477569177223 1107 28-29 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3 -0.10148135829696514 2216 4 -0.12069607335394039 2216 5 -1.8435797642979423E-4 2216 6 -0.03419682570117999 2216 7 0.011685173305771457 2216 8 -0.046491542160019605 2216 9 -0.10168891356262577 2216 10-11 -0.10272412574872902 2216 12-13 -0.23441300903145645 2216 14-15 -0.19812462081301874 2216 16-17 -0.2776606824377481 2216 18-19 -0.21742087583906766 2216 20-21 -0.2131012015788798 2216 22-23 -0.3248381856277973 2216 24-25 -0.3001271510221599 2216 26-27 -0.2893517815729254 2216 28-29 -0.229325443748003 2216 30-31 -0.29790629203149877 2216 32-33 -0.4191185358298384 2216 34-35 -0.3417573349492642 2216 36-37 -0.30742857191143713 2216 38-39 -0.38529063710440425 2216 40-41 -0.3759235450962919 2216 42-43 -0.32319677866453134 2216 44-45 -0.37156086138147515 2216 46-47 -0.40108111837810156 2216 48-49 -0.45346801122781955 2216 50-51 -0.42427780629881795 2216 52-53 -0.4048467281333572 2216 54-55 -0.5052569013875967 2216 56-57 -0.5173958496735693 2216 58-59 -0.4662061160222777 2216 60-61 -0.4963914354613337 2216 62-63 -0.7005647376773751 2216 64-65 -0.7303767490437068 2216 66-67 -0.6858113510261958 2216 68-69 -0.7177368031162601 2216 70-71 -0.7218523284597609 2216 72-73 -0.6846162461773773 2216 74-75 -0.7154127557288987 2216 76-77 -0.5033767878425692 2216 78-79 -0.5016868848185823 2216 80-81 -0.6203838510534041 2216 82-83 -0.6362674882144361 2216 84-85 -0.6474244926415977 2216 86-87 -0.7629547264350549 2216 88-89 -1.3703344331396945 2216 90-91 -0.8021748660027361 2216 92-93 -0.34040582078899373 2216 94-95 -0.11514668480618395 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11866.0 3 1883.0 4 1262.0 5 1137.0 6 1107.0 7 1134.0 8 1343.0 9 1573.0 10 1955.0 11 2478.0 12 2891.0 13 3577.0 14 4131.0 15 4643.0 16 5122.0 17 5705.0 18 7455.0 19 8762.0 20 9594.0 21 13404.0 22 15049.0 23 19793.0 24 29575.0 25 50549.0 26 92165.0 27 262112.0 28 420298.0 29 15318.0 30 643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.708302057953446 20.333378824794988 14.733312995974005 24.225006121277563 2 12.92368735568514 20.044976563417254 45.478912092995714 21.552423987901886 3 19.30594067556687 25.172726743380565 29.375292395605985 26.14604018544658 4 11.374064041733348 17.5304556653461 39.947572281378825 31.14790801154173 5 12.005260885716295 38.91559491179984 35.19713261648745 13.882011585996407 6 29.326137654486995 37.29844941014968 20.024304482380757 13.351108452982569 7 27.803143727597128 31.94935596132155 22.31045112812135 17.937049182959967 8 23.500588044041088 39.991510490464854 20.67938153019897 15.828519935295086 9 25.194977742633395 18.748268982984857 22.27221823056946 33.78453504381229 10-11 23.65841677801265 26.760366214977445 30.573090213578574 19.00812679343133 12-13 26.27241148676347 24.534785878825314 29.27034375694616 19.922458877465058 14-15 20.220135189920164 30.18060779268738 25.843130722391034 23.756126295001426 16-17 19.689017781880946 30.77324087825633 30.14590775900132 19.391833580861398 18-19 20.9591931457531 28.644329314951637 30.888668665403866 19.507808873891392 20-21 21.673764829373145 28.869635986681413 28.5714429072897 20.885156276655742 22-23 20.396500217256268 31.103872580452556 28.84957537186948 19.650051830421695 24-25 21.256086155476435 28.923036474786358 30.594797516166196 19.22607985357101 26-27 19.35402458947301 30.718928997194244 29.935004074161785 19.99204233917096 28-29 19.410845323064187 30.935688212641587 27.876318661331396 21.777147802962833 30-31 20.5183718605874 28.94676896893602 29.52101504830792 21.01384412216866 32-33 19.43310067099277 30.24334463780289 30.520512368936142 19.8030423222682 34-35 19.843395486105187 30.56853680246216 30.091678767073116 19.496388944359538 36-37 19.21204065935224 29.829753136780017 29.906720714564184 21.051485489303566 38-39 20.669948741826587 30.602424025914075 28.73066780127724 19.996959430982095 40-41 22.441105281960095 28.172929101557013 30.209508250679363 19.176457365803536 42-43 19.50058403008859 30.765591194993796 30.263847132633032 19.46997764228458 44-45 19.225879155946068 30.86714419321562 29.279324933468736 20.627651717369577 46-47 21.200643436585572 28.91907269669371 29.178424202527985 20.701859664192735 48-49 20.34277164562602 29.538791609028785 29.083007074093086 21.035429671252107 50-51 19.057594197430266 31.133419767110475 29.317808703051806 20.49117733240745 52-53 19.183782829113998 30.043982884506544 30.217636504489604 20.55459778188985 54-55 19.62576447938634 29.0998648786291 30.312545314329036 20.961825327655525 56-57 20.262733260814592 29.65372635280234 28.032741810533413 22.050798575849655 58-59 20.623938811308108 30.276340559785815 27.587193083157054 21.512527545749023 60-61 19.866204928330877 28.27362913487282 31.032117640919836 20.828048295876467 62-63 20.426853743612796 28.36835841384653 30.345832112422784 20.85895573011789 64-65 20.98415140235027 29.61174523230009 28.41167318181659 20.992430183533052 66-67 20.753238256178477 31.15047906523074 27.518153100176214 20.57812957841457 68-69 21.565691738633923 28.528034616534775 30.047224435484353 19.859049209346946 70-71 22.38807093456856 28.50854570487013 29.025191565883006 20.0781917946783 72-73 21.16471856172054 30.074689621123024 28.52334715470977 20.237244662446667 74-75 20.316670747518373 30.565244790893143 29.883826179800987 19.234258281787493 76-77 20.781988190951747 28.93081350775295 30.27067085188114 20.016527449414166 78-79 20.2884938142266 28.875321681791792 29.561057700268083 21.27512680371352 80-81 20.88394258442346 29.796322015325273 29.27490958571996 20.04482581453131 82-83 20.420142726903716 29.52599475072465 30.517195616538977 19.536666905832657 84-85 20.145325150222174 29.341189976357818 30.391641345316316 20.12184352810369 86-87 19.131540378610783 31.37088734048729 28.199423689540964 21.298148591360963 88-89 29.13535119521838 18.6892307921763 35.560620994032924 16.6147970185724 90-91 30.85614130619746 15.566415954803798 17.469148827553347 36.108293911445394 92-93 22.754253689897173 17.83276393072923 36.27450815395749 23.13847422541611 94-95 16.091634382159505 7.180474257365457 28.178899336861228 48.54899202361381 96-97 27.216762794772574 7.406390693825898 15.522912559927965 49.853933951473564 98-99 57.16416181623375 14.102452726377667 18.805200822401762 9.928184634986824 100-101 30.940180356972096 7.242550608465718 12.826216203855765 48.991052830706415 102-103 23.118894573346445 2.129442268037928 37.809050022632015 36.94261313598361 104-105 40.71114822013195 4.72667275236853 42.159731696452646 12.402447331046867 106-107 6.061219022835494 1.947057955247462 7.113062208542672 84.87866081337437 108-109 18.343922570259544 11.397739087402396 22.308011594482462 47.9503267478556 110-111 40.45112484614003 2.8503022823180086 25.503927445652625 31.194645425889338 112-113 27.46587831370297 2.829011305374839 39.329251388980424 30.37585899194177 114-115 29.01096134922996 4.043952188074479 24.415890523810614 42.52919593888495 116-117 20.110874659592064 4.298150244585252 20.79999042894765 54.79098466687503 118-119 20.83389165518757 4.369382165502677 16.157030478120035 58.63969570118972 120-121 25.821221766307474 4.269662921348314 30.452889519499944 39.456225792844265 122-123 34.47321354296648 1.6801846768155728 34.28146576823892 29.56513601197904 124-125 38.96659091639097 2.1019697748369897 20.102162496386473 38.82927681238557 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16.0 1 22.5 2 53.5 3 92.0 4 125.5 5 132.0 6 113.0 7 103.0 8 91.0 9 83.5 10 100.0 11 105.5 12 94.5 13 101.0 14 114.0 15 136.0 16 193.5 17 274.5 18 387.5 19 561.0 20 821.5 21 1194.5 22 1700.5 23 2445.5 24 3418.0 25 4698.0 26 6400.0 27 8392.0 28 11023.0 29 14681.0 30 19301.0 31 25096.0 32 32012.5 33 39768.5 34 46891.5 35 52048.0 36 54466.0 37 53871.5 38 51706.0 39 49334.5 40 46903.0 41 43869.5 42 40509.0 43 37962.5 44 36240.0 45 34312.5 46 31976.5 47 30074.0 48 28555.0 49 26734.0 50 24815.0 51 22385.5 52 19496.5 53 16790.5 54 14371.5 55 12239.0 56 10205.5 57 8319.5 58 6721.5 59 5415.5 60 4218.5 61 3186.5 62 2454.5 63 1818.0 64 1296.5 65 956.0 66 706.5 67 491.0 68 365.5 69 269.5 70 186.5 71 135.5 72 96.0 73 72.0 74 53.5 75 37.5 76 28.5 77 18.0 78 10.5 79 8.5 80 9.5 81 6.0 82 2.5 83 2.5 84 2.0 85 4.5 86 5.0 87 3.5 88 2.5 89 0.5 90 3.5 91 4.0 92 1.0 93 1.5 94 1.5 95 1.5 96 3.5 97 2.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 5.01744062360766E-4 3 0.04435417511269171 4 0.048769522861466456 5 0.04957231336124369 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.519184685968426E-4 12-13 0.0020571506556791405 14-15 0.0 16-17 4.013952498886128E-4 18-19 0.006171451967037422 20-21 0.0022076738743873705 22-23 5.01744062360766E-4 24-25 0.0 26-27 0.0 28-29 0.0011540113434297618 30-31 0.0 32-33 0.0015554065933183745 34-35 1.003488124721532E-4 36-37 5.01744062360766E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 5.01744062360766E-5 50-51 0.0 52-53 0.0 54-55 0.001254360155901915 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.01744062360766E-5 66-67 0.0 68-69 6.020928748329192E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.512208436525362E-4 80-81 0.0 82-83 5.519184685968426E-4 84-85 0.0 86-87 0.001254360155901915 88-89 19.261252112342504 90-91 26.286271078268058 92-93 40.37373911717129 94-95 44.78943808678968 96-97 48.45708683383441 98-99 93.06930891779827 100-101 51.727605155520585 102-103 53.1111644074804 104-105 74.88133752925168 106-107 81.64449626903115 108-109 12.6030582304089 110-111 39.10001164046225 112-113 52.03743211402836 114-115 86.84457173133812 116-117 66.4492275148416 118-119 71.91673256238685 120-121 80.37403012872745 122-123 75.87413850544492 124-125 50.013446740871274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 996524.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.17346130844754 #Duplication Level Percentage of deduplicated Percentage of total 1 88.91017977104397 69.50416498258822 2 8.000477376876855 12.508500173407855 3 1.5884067100707202 3.7251375147537575 4 0.5400836772510319 1.6888084178763043 5 0.2670296103536122 1.0437314456593965 6 0.1499424711829185 0.7032913181706535 7 0.10650942918044182 0.5828347518715472 8 0.07024528152205164 0.43930534377320873 9 0.04772043268427513 0.3357424258259902 >10 0.27664761063162124 3.89428135210357 >50 0.02517324498155958 1.3833820791435494 >100 0.015402381831597154 2.4761433151093786 >500 0.0014118838989696876 0.7342738256050846 >1k 7.701184903471025E-4 0.9804030541114077 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2331 0.23391308187258913 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1963 0.19698471888283672 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1786 0.17922297907526563 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1325 0.132962176525603 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1271 0.12754334065210673 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1095 0.10988194965700776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.003488124721532E-4 0.0 10-11 0.0 0.0 0.0 2.006976249443064E-4 0.0 12-13 0.0 0.0 0.0 2.006976249443064E-4 0.0 14-15 0.0 0.0 0.0 2.006976249443064E-4 0.0 16-17 0.0 0.0 0.0 3.010464374164596E-4 0.0 18-19 0.0 0.0 0.0 4.515696561246894E-4 0.0 20-21 0.0 0.0 0.0 6.020928748329192E-4 0.0 22-23 0.0 0.0 0.0 7.024416873050724E-4 0.0 24-25 0.0 0.0 0.0 9.031393122493788E-4 0.0 26-27 0.0 0.0 0.0 0.001154011343429762 0.0 28-29 0.0 0.0 0.0 0.001756104218262681 0.0 30-31 0.0 0.0 0.0 0.003763080467705745 0.0 32-33 0.0 0.0 0.0 0.0085798234663691 0.0 34-35 0.0 0.0 0.0 0.012944996808907764 0.0 36-37 0.0 0.0 0.0 0.023130401274831312 0.0 38-39 0.0 0.0 0.0 0.04023987380133344 0.0 40-41 0.0 0.0 0.0 0.06101207798306915 0.0 42-43 0.0 0.0 0.0 0.08424282807037262 0.0 44-45 0.0 0.0 0.0 0.10877811271981408 0.0 46-47 0.0 0.0 0.0 0.13652455936836444 0.0 48-49 0.0 0.0 0.0 0.1698403651091193 0.0 50-51 0.0 0.0 0.0 0.20400913575588747 0.0 52-53 0.0 0.0 0.0 0.24299464940131899 0.0 54-55 0.0 0.0 0.0 0.2836860928587771 0.0 56-57 0.0 0.0 0.0 0.334663289594631 0.0 58-59 0.0 0.0 0.0 0.39231368235988295 0.0 60-61 0.0 0.0 0.0 0.45493134134250657 0.0 62-63 0.0 0.0 0.0 0.5211113831678915 0.0 64-65 0.0 0.0 0.0 0.5896997964926083 0.0 66-67 1.003488124721532E-4 0.0 0.0 0.6718352995010657 0.0 68-69 1.003488124721532E-4 0.0 0.0 0.7753952739723278 0.0 70-71 1.003488124721532E-4 0.0 0.0 0.8895420481594021 0.0 72-73 1.003488124721532E-4 0.0 0.0 0.9957110917549402 0.0 74-75 1.003488124721532E-4 0.0 0.0 1.1031846699126162 0.0 76-77 1.003488124721532E-4 0.0 0.0 1.226011616378532 0.0 78-79 1.003488124721532E-4 0.0 0.0 1.2622375376809791 0.0 80-81 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 82-83 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 84-85 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 86-87 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 88-89 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 90-91 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 92-93 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 94-95 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 96-97 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 98-99 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 100-101 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 102-103 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 104-105 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 106-107 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 108-109 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 110-111 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 112-113 1.003488124721532E-4 0.0 0.0 1.2630905025869923 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTACG 5 2.1961442E-4 6096.268 110-111 CGACGCC 40 0.0 3156.3518 108-109 AACGTCG 10 0.0012806734 2525.0815 108-109 CGTCGCG 20 1.3440404E-6 2525.0815 108-109 CCGCTCG 45 1.8189894E-12 2244.5168 108-109 CGCGTCG 15 0.0019761007 2032.0895 110-111 CGTTTCG 15 0.0019761007 2032.0895 110-111 CGGCGCG 25 2.6246944E-6 2020.0653 108-109 CCGCTCC 240 0.0 1999.0227 108-109 CCGCGCC 175 0.0 1731.4843 108-109 CCGCGCG 75 0.0 1683.3877 108-109 AACCGCG 15 0.002881137 1683.3876 108-109 CCGTTCG 30 4.5348042E-6 1683.3876 108-109 CGCGGCG 50 1.0293661E-8 1515.049 108-109 CGTCGCC 90 2.910383E-11 1354.7262 110-111 CGGCGCA 20 0.0051213494 1262.5408 108-109 GCGCGTT 20 0.0051213494 1262.5408 108-109 CATCGCG 40 1.0746002E-5 1262.5408 108-109 GCTCGCG 20 0.0051213494 1262.5408 108-109 CGGCGCC 140 0.0 1262.5406 108-109 >>END_MODULE