FastQCFastQC Report
Fri 27 May 2016
SRR939916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996524
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.22759110668684346No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22320.22397854943784595No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19630.19698471888283672No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19340.19407460332114432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.1693887954529946No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA13290.13336357177549163No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA12910.1295503169015498No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA11300.11339415809353312No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC705.4095908E-542.5555573
TAGGACC18700.034.7271544
GTACATG39650.030.8062361
TACATGG39250.030.5098952
TAGCCCT7650.029.5941934
ACATGGG38000.028.3778383
GTATCAA63950.028.3244171
GTATAGC6100.027.3499261
ATAGCCC4750.026.3396513
AGAGTAC53550.024.99751310-11
GAGTACT34000.024.93501912-13
GGTATCA48750.024.6890681
TCAACGC72200.024.6727944
CAACGCA72250.024.6557185
ATCAACG72250.024.6557183
AACGCAG73050.024.3514446
AGTACTT34850.024.32684712-13
TATCAAC73350.024.2859672
CTATACT5850.023.4237444
TATAGCC5900.023.2252372
Produced by FastQC (version 0.11.5)