FastQCFastQC Report
Fri 27 May 2016
SRR939915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815463
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA28370.1562686763652027No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26650.1467945091692863No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC22670.12487172693687505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4500.02649.8845108-109
CCGCTCG750.01987.4135108-109
CCGCGCC3900.01465.0804108-109
CGTCGGT501.2671269E-51196.2699110-111
CGACGCT451.6130221E-51104.1185108-109
CGTCGCG551.6863354E-51087.5182110-111
CCGTTCG704.2526153E-81064.6858108-109
CGGCGCG1001.4188117E-10993.7067108-109
CGACGCC755.603397E-8993.7067108-109
CTCCGCC3250.0920.20764110-111
CGGCGCC2700.0920.0988108-109
CGTCTCG1102.2737368E-10903.3698108-109
CGCCGCC3200.0841.1274110-111
CCGCGCG1551.8189894E-12801.37634108-109
CGTCGCC2250.0772.883108-109
CCGTTCC2050.0727.1025108-109
CCGTGCG1102.5899317E-7677.52734108-109
CCGCGCT757.4609685E-5662.4711108-109
CAGCGCG1203.666937E-7621.0667108-109
CCACGCG1203.666937E-7621.0667108-109
Produced by FastQC (version 0.11.5)