##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939915_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1815463 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926532790808736 34.0 31.0 34.0 30.0 34.0 2 32.000837251984755 34.0 31.0 34.0 30.0 34.0 3 32.14755960325272 34.0 31.0 34.0 30.0 34.0 4 35.41863755967486 37.0 35.0 37.0 33.0 37.0 5 35.4475062284387 37.0 35.0 37.0 33.0 37.0 6 35.49937013312857 37.0 37.0 37.0 35.0 37.0 7 35.48272314004747 37.0 37.0 37.0 35.0 37.0 8 35.46757438736014 37.0 37.0 37.0 35.0 37.0 9 37.180403566473124 39.0 39.0 39.0 35.0 39.0 10-11 37.15087638800681 39.0 39.0 39.0 35.0 39.0 12-13 37.099852214008216 39.0 39.0 39.0 35.0 39.0 14-15 38.622730950727174 41.0 39.5 41.0 35.0 41.0 16-17 38.610451713970484 41.0 39.5 41.0 35.0 41.0 18-19 38.61032943111482 41.0 39.5 41.0 35.0 41.0 20-21 38.649828445966676 41.0 39.5 41.0 35.0 41.0 22-23 38.620731185378055 41.0 39.0 41.0 34.0 41.0 24-25 38.6296873029084 41.0 39.0 41.0 34.0 41.0 26-27 38.58205124532971 41.0 39.0 41.0 34.0 41.0 28-29 38.52328249047213 41.0 39.0 41.0 34.0 41.0 30-31 38.44256726796415 41.0 39.0 41.0 34.0 41.0 32-33 38.34685256598455 41.0 39.0 41.0 34.0 41.0 34-35 38.28854870630798 40.5 39.0 41.0 34.0 41.0 36-37 38.455898853350355 41.0 39.0 41.0 34.0 41.0 38-39 38.417006019951934 41.0 39.0 41.0 34.0 41.0 40-41 38.34890768911292 41.0 39.0 41.0 34.0 41.0 42-43 38.28566128860792 41.0 39.0 41.0 33.0 41.0 44-45 38.19469991952466 41.0 39.0 41.0 33.0 41.0 46-47 38.095915752620684 41.0 38.0 41.0 33.0 41.0 48-49 38.01304515707563 40.5 38.0 41.0 33.0 41.0 50-51 37.90245986836416 40.0 38.0 41.0 33.0 41.0 52-53 37.800900100965976 40.0 38.0 41.0 33.0 41.0 54-55 37.663900338370986 40.0 38.0 41.0 32.5 41.0 56-57 37.48435302729938 40.0 38.0 41.0 32.0 41.0 58-59 37.32833001829285 40.0 37.0 41.0 32.0 41.0 60-61 37.12247509313051 40.0 37.0 41.0 31.0 41.0 62-63 36.88329340779735 40.0 36.5 41.0 31.0 41.0 64-65 36.603725606085064 39.5 36.0 41.0 31.0 41.0 66-67 36.29898295916799 39.0 35.5 41.0 30.5 41.0 68-69 35.91057377649669 39.0 35.0 41.0 30.0 41.0 70-71 35.50018507675452 38.0 35.0 40.0 29.5 41.0 72-73 35.103280265144484 37.0 35.0 39.5 29.0 41.0 74-75 34.67521590910968 37.0 35.0 39.0 29.0 41.0 76-77 34.19781950940339 36.0 34.0 39.0 29.0 40.5 78-79 33.72840289226495 36.0 34.0 38.0 28.0 39.5 80-81 33.28500360514094 35.0 34.0 37.0 27.5 39.0 82-83 33.066171274214895 35.0 34.0 37.0 28.0 39.0 84-85 32.6356001196389 35.0 34.0 36.5 27.5 38.0 86-87 31.984002428030756 35.0 34.0 36.0 14.0 37.0 88-89 16.436352324448364 18.0 16.5 18.5 2.0 19.0 90-91 2.7933034162635098 2.0 2.0 2.0 2.0 2.0 92-93 2.3080635628487056 2.0 2.0 2.0 2.0 2.0 94-95 2.106333205358633 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11054574484680302 1101 2 0.19320904491950586 1101 3 0.19637890474528064 1101 4 0.120506918630916 1101 5 0.09445248633291214 1101 6 0.10940683485507918 1101 7 0.10519051231445076 1101 8 0.15591756829012837 1101 9 0.14041157138566973 1101 10-11 0.11284173396821018 1101 12-13 0.13256268941825766 1101 14-15 0.19675100466137252 1101 16-17 0.20641041229295354 1101 18-19 0.143846701552512 1101 20-21 0.18353831396233034 1101 22-23 0.17648480341913597 1101 24-25 0.13376375698241816 1101 26-27 0.16127306209505576 1101 28-29 0.20719689684967335 1101 30-31 0.1603039736575198 1101 32-33 0.19624935876406369 1101 34-35 0.2236609739754769 1101 36-37 0.18583547902410658 1101 38-39 0.14037610348449903 1101 40-41 0.11735336275258845 1101 42-43 0.16283893421731221 1101 44-45 0.1467400210661154 1101 46-47 0.14935021356268408 1101 48-49 0.15232408866076952 1101 50-51 0.22202994072235782 1101 52-53 0.06182400069825178 1101 54-55 0.06821758746211515 1101 56-57 0.042151195005786235 1101 58-59 0.032624261502064655 1101 60-61 0.04904487347171482 1101 62-63 0.022811248413702856 1101 64-65 0.034174935271266804 1101 66-67 -0.028463976496375665 1101 68-69 -0.02262907266000269 1101 70-71 0.02356331635967024 1101 72-73 0.05914337765363342 1101 74-75 -0.02051871379665471 1101 76-77 -0.08246512949583717 1101 78-79 -0.10647171958920865 1101 80-81 -0.007460541281787414 1101 82-83 0.007963678079541125 1101 84-85 0.08323298917252231 1101 86-87 -0.024021633861590175 1101 88-89 0.4257262349642019 1101 90-91 -0.04920095265508273 1101 92-93 -0.12602332182403808 1101 94-95 0.02128912289468099 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.019263585035623976 1102 2 -0.025734338312762617 1102 3 0.03605091932462301 1102 4 0.017888716335114907 1102 5 0.056682386907979776 1102 6 -0.053025135270203805 1102 7 0.022463511944003756 1102 8 0.08299569604830026 1102 9 0.1116705809154439 1102 10-11 0.11380484306712191 1102 12-13 0.05516961659366615 1102 14-15 0.039284531673182244 1102 16-17 0.12797731216939212 1102 18-19 0.03901605212049475 1102 20-21 0.07317778981219192 1102 22-23 0.0323966585804456 1102 24-25 -0.014094467391231547 1102 26-27 0.06941610446315138 1102 28-29 0.08521379958529707 1102 30-31 0.08032815860430276 1102 32-33 0.12318204119850407 1102 34-35 0.15038605496490476 1102 36-37 0.10856190134262533 1102 38-39 0.1365098373518805 1102 40-41 0.07429362201820311 1102 42-43 0.04933082408660283 1102 44-45 0.11202707046531657 1102 46-47 0.11343516357672456 1102 48-49 0.0761738274807513 1102 50-51 0.06243586370346321 1102 52-53 0.12391190561430676 1102 54-55 0.10833423585489044 1102 56-57 0.025020501815859575 1102 58-59 0.13927251131000418 1102 60-61 0.161160997266812 1102 62-63 0.2224466095283404 1102 64-65 0.20344687576051257 1102 66-67 0.09759873778685346 1102 68-69 0.11840876052788474 1102 70-71 0.08866439464406284 1102 72-73 0.06890297642343768 1102 74-75 0.16575152734423426 1102 76-77 0.03428084838154888 1102 78-79 0.10577076760041848 1102 80-81 0.09135526029598395 1102 82-83 0.07821345866843643 1102 84-85 0.10537703971289858 1102 86-87 0.28531089019904954 1102 88-89 0.9695890327852581 1102 90-91 0.02340034939134572 1102 92-93 -0.028995751854892227 1102 94-95 0.0820325909078976 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.050806010290166626 1103 2 0.16611589154390316 1103 3 0.1359469976226464 1103 4 0.17459587189910053 1103 5 0.06780758690654665 1103 6 -0.003027879471289907 1103 7 0.045168345675243415 1103 8 0.04427665928674429 1103 9 0.07904143344327252 1103 10-11 0.03854497915563826 1103 12-13 0.031845697063182854 1103 14-15 -0.017344816841550426 1103 16-17 0.03933685799218267 1103 18-19 -0.020053771093301975 1103 20-21 0.13493432011592432 1103 22-23 0.08481045188960934 1103 24-25 0.11967638704560102 1103 26-27 0.12907794431873043 1103 28-29 0.08432021910682153 1103 30-31 0.15067239775069652 1103 32-33 0.21687011024737046 1103 34-35 0.17144794743756364 1103 36-37 0.20039517382627992 1103 38-39 0.2430187691298471 1103 40-41 0.14029829491539658 1103 42-43 0.13459412123227565 1103 44-45 0.2007423190362445 1103 46-47 0.1955064626934515 1103 48-49 0.14176604993762254 1103 50-51 0.11053238739724947 1103 52-53 0.08938038548200922 1103 54-55 0.18488931793632446 1103 56-57 0.15192794528233122 1103 58-59 0.17145460754554875 1103 60-61 0.17582852835682417 1103 62-63 0.00515442906967678 1103 64-65 0.06572869686007721 1103 66-67 0.17703478358399138 1103 68-69 0.062194696139385997 1103 70-71 0.14946276625900623 1103 72-73 0.23006774362570326 1103 74-75 0.1715928725516065 1103 76-77 0.17520332947341188 1103 78-79 0.11611364657757406 1103 80-81 0.0790048639975538 1103 82-83 0.18439867911727248 1103 84-85 0.1438291134340801 1103 86-87 0.22203982935056388 1103 88-89 0.732039875791898 1103 90-91 -0.5799009990852051 1103 92-93 -0.32494590761813225 1103 94-95 -0.11598142778369125 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.02472798124370712 1104 2 0.17401010336782008 1104 3 0.1829018959542168 1104 4 0.15432931901447944 1104 5 0.17316456593502494 1104 6 0.10588949699825179 1104 7 0.1957282028457712 1104 8 0.1598997980110397 1104 9 0.1200261238362117 1104 10-11 0.20407615538722013 1104 12-13 0.23427720575775624 1104 14-15 0.2759343486661834 1104 16-17 0.3060939095421418 1104 18-19 0.26004053596967935 1104 20-21 0.21459852231399879 1104 22-23 0.11183206125507894 1104 24-25 0.2414064032245875 1104 26-27 0.2733349121340396 1104 28-29 0.24065500044287802 1104 30-31 0.30868580403268453 1104 32-33 0.27865477967024077 1104 34-35 0.3560412081814448 1104 36-37 0.29709894101191026 1104 38-39 0.3174266195083959 1104 40-41 0.3043667359800395 1104 42-43 0.2978284195286065 1104 44-45 0.21868461639474646 1104 46-47 0.23472675473080074 1104 48-49 0.12091430104837997 1104 50-51 0.09971998651359826 1104 52-53 0.13839566596396935 1104 54-55 0.224886449114301 1104 56-57 0.17523741545313953 1104 58-59 0.15743240257186386 1104 60-61 0.19916974488298678 1104 62-63 0.10430448593642438 1104 64-65 0.08812196430273644 1104 66-67 0.12969499710007426 1104 68-69 0.1090706654590079 1104 70-71 0.12198884414242173 1104 72-73 0.08618721768470294 1104 74-75 0.030501802743117423 1104 76-77 0.027271502587161933 1104 78-79 0.011048209766656214 1104 80-81 0.07904818757883447 1104 82-83 0.11834499865161519 1104 84-85 0.15423369015176291 1104 86-87 0.2912756450644238 1104 88-89 0.7843875770208335 1104 90-91 -0.6368890563393204 1104 92-93 -0.32494590761813225 1104 94-95 -0.11598142778369125 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.009162762218714704 1105 2 -0.06893110795722635 1105 3 -4.264514913501216E-4 1105 4 0.031343942840088346 1105 5 0.03191523252654349 1105 6 0.02203871522550571 1105 7 0.051038606876105064 1105 8 0.04449224865436463 1105 9 0.161335432416422 1105 10-11 0.06825031172443374 1105 12-13 0.08382507967404962 1105 14-15 0.08554750158263857 1105 16-17 0.09783814650621991 1105 18-19 0.16126533013922284 1105 20-21 0.1540252608352617 1105 22-23 0.002659111647226098 1105 24-25 0.12780815305442417 1105 26-27 0.16582435867159262 1105 28-29 0.14647808380258454 1105 30-31 0.11113380266843365 1105 32-33 0.09996186266576501 1105 34-35 0.19672561882783413 1105 36-37 0.1424947988230656 1105 38-39 0.13033103642689525 1105 40-41 0.185908329122924 1105 42-43 0.14499889208960326 1105 44-45 0.059456532984455635 1105 46-47 0.14641540988512247 1105 48-49 0.1389093816391167 1105 50-51 0.029019808900379473 1105 52-53 0.009186817964177862 1105 54-55 0.04742605801838096 1105 56-57 0.06533045645447544 1105 58-59 0.08825494398724487 1105 60-61 0.008996785158842613 1105 62-63 0.09651038720429028 1105 64-65 0.1444837403193162 1105 66-67 0.11774028341287135 1105 68-69 0.1761432845530777 1105 70-71 0.12706527274379198 1105 72-73 0.12810299809175518 1105 74-75 0.10871123315241249 1105 76-77 0.1248579716103464 1105 78-79 0.03717744108728027 1105 80-81 -0.036074877137991734 1105 82-83 0.03977839080397416 1105 84-85 0.05643550154646704 1105 86-87 0.2322481533128169 1105 88-89 0.9969579394587456 1105 90-91 -0.7840373453127043 1105 92-93 -0.32494590761813225 1105 94-95 -0.11598142778369125 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.12932833901132668 1106 2 -0.11457890156352235 1106 3 -0.09939508048492485 1106 4 -0.12179656029767472 1106 5 -4.706118681241378E-5 1106 6 -0.0750224417224743 1106 7 -0.12779529990216787 1106 8 -0.025761394920152725 1106 9 -0.01359639319657191 1106 10-11 -0.1631799479108551 1106 12-13 -0.16949505031787027 1106 14-15 -0.18080501518502246 1106 16-17 -0.2689307195002044 1106 18-19 -0.19430911894699676 1106 20-21 -0.1929537383000124 1106 22-23 -0.2948693904747657 1106 24-25 -0.35120114820583126 1106 26-27 -0.463750752656388 1106 28-29 -0.2851736699762526 1106 30-31 -0.17661231807201716 1106 32-33 -0.17973154761375554 1106 34-35 -0.2883544393963291 1106 36-37 -0.24423758579500543 1106 38-39 -0.24673660185965218 1106 40-41 -0.23051684336699196 1106 42-43 -0.4334850556016008 1106 44-45 -0.2606211766880904 1106 46-47 -0.3624215342425856 1106 48-49 -0.24290784959280387 1106 50-51 -0.2754385802421737 1106 52-53 -0.2662333487082975 1106 54-55 -0.23017108322169122 1106 56-57 -0.3433195993962386 1106 58-59 -0.22637234170120024 1106 60-61 -0.06364565906098107 1106 62-63 -0.1294074151489113 1106 64-65 -0.1031210274894292 1106 66-67 -0.16158466152479178 1106 68-69 -0.22217708610314446 1106 70-71 -0.2981283790299045 1106 72-73 -0.28950806848262545 1106 74-75 -0.28982449055833825 1106 76-77 -0.20689880308969322 1106 78-79 -0.1474201682033609 1106 80-81 -0.14234061367862694 1106 82-83 -0.13097521210656993 1106 84-85 -0.18009310679976664 1106 86-87 -0.26824292634042735 1106 88-89 0.5040635529120436 1106 90-91 -0.26111158112056465 1106 92-93 -0.3087523122760656 1106 94-95 -0.11598142778369125 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.05197706719400941 1107 2 0.0341028244589765 1107 3 0.04935430817719322 1107 4 0.081798080082649 1107 5 0.08150725911889367 1107 6 0.07766496532383371 1107 7 0.09152226500253136 1107 8 0.05000242791083309 1107 9 0.10818778487777791 1107 10-11 0.17520500159672991 1107 12-13 0.1326820115988525 1107 14-15 0.1513944743247606 1107 16-17 0.18945827393823578 1107 18-19 0.1907378597644609 1107 20-21 0.1604106380720367 1107 22-23 0.16123767055034222 1107 24-25 0.1873533008303525 1107 26-27 0.14757400796559494 1107 28-29 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0.0 2215 116-117 0.0 2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 0.029371921335901163 2216 2 0.004274699683143979 2216 3 -0.018662651242962625 2216 4 -0.03480110308658624 2216 5 -0.07089207977379175 2216 6 -0.054458740173565445 2216 7 -0.011902260673210208 2216 8 -0.015564461827466403 2216 9 -0.18536403876702678 2216 10-11 -0.12917111423874417 2216 12-13 -0.0351529887197799 2216 14-15 -0.10608555060077407 2216 16-17 -0.11619942964026109 2216 18-19 -0.12903260106995873 2216 20-21 -0.108393146268007 2216 22-23 -0.17819712962550938 2216 24-25 -0.13236794730164547 2216 26-27 -0.18696193182074694 2216 28-29 -0.1386899431295845 2216 30-31 -0.20561597740439197 2216 32-33 -0.24151387875095054 2216 34-35 -0.24436060393988868 2216 36-37 -0.3002438954969904 2216 38-39 -0.24633322891369147 2216 40-41 -0.3018618769164547 2216 42-43 -0.2948172169480969 2216 44-45 -0.3538517467855442 2216 46-47 -0.320460810094076 2216 48-49 -0.222430014838487 2216 50-51 -0.2940060742225654 2216 52-53 -0.33877320363026087 2216 54-55 -0.3042708887987189 2216 56-57 -0.38886707669904297 2216 58-59 -0.37546328144794217 2216 60-61 -0.38507108804881085 2216 62-63 -0.40196927832212737 2216 64-65 -0.3936065511741518 2216 66-67 -0.38329786151782486 2216 68-69 -0.47242357628395837 2216 70-71 -0.44874554801174327 2216 72-73 -0.4066378575383922 2216 74-75 -0.39533183490494395 2216 76-77 -0.46375335166261067 2216 78-79 -0.44914240598359356 2216 80-81 -0.43431317382223256 2216 82-83 -0.45891819878817586 2216 84-85 -0.45488705999085965 2216 86-87 -0.48305300551653474 2216 88-89 -1.1218780786597797 2216 90-91 -0.7840373453127043 2216 92-93 -0.32494590761813225 2216 94-95 -0.11598142778369125 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19953.0 3 3469.0 4 2348.0 5 1942.0 6 2021.0 7 2248.0 8 2465.0 9 2859.0 10 3481.0 11 4244.0 12 4807.0 13 5863.0 14 6661.0 15 7575.0 16 8313.0 17 9531.0 18 12006.0 19 14714.0 20 16746.0 21 23163.0 22 27711.0 23 35788.0 24 53977.0 25 89528.0 26 169831.0 27 500932.0 28 754749.0 29 27504.0 30 1034.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.07839157283845 20.05703228322472 14.734808696183837 24.129767447752997 2 13.330494741260313 20.593746798321522 43.82501567101122 22.250742789406946 3 18.65450637864051 25.58135177582454 29.315901137960488 26.44824070757446 4 11.546460952431339 17.930171347701588 40.09879000703202 30.424577692835054 5 12.736062483018824 38.48705076690168 35.481609625524044 13.295277124555458 6 29.4900529506798 37.98876650198875 19.736563069586104 12.784617477745346 7 27.45817458136024 31.775144963020452 22.92076456529271 17.845915890326598 8 23.483265701366538 38.67266917585211 21.191949381507637 16.65211574127371 9 25.079828120980707 18.09797280363191 21.800664623845268 35.02153445154212 10-11 23.534819594365146 27.084379331741626 30.101096119117543 19.279704954775685 12-13 25.557837542768898 24.548900519464997 29.283796002645108 20.609465935120998 14-15 20.72630017216008 28.608318050258845 26.33021961633447 24.335162161246604 16-17 20.568864872903937 30.036230536930773 29.038713382164 20.356191208001288 18-19 21.282823967765935 28.58498363810986 29.74558851436902 20.38660387975519 20-21 22.112874591071012 28.427470773867984 28.322976644983815 21.13667799007719 22-23 21.185437750196577 29.7953534872256 28.3339231787204 20.685285583857425 24-25 21.675655190989847 28.552606139590836 29.34620534816738 20.425533321251933 26-27 20.481194053527943 29.566011535349386 28.995909032571852 20.956885378550815 28-29 20.569125462802432 29.65028482162259 27.901502367615027 21.879087347959953 30-31 21.294567832007594 28.527185627027375 28.700722625578155 21.477523915386872 32-33 20.56212834412824 29.33711093098944 29.17761787159528 20.92314285328704 34-35 20.903693082998455 29.507979100630887 29.06952309371474 20.51880472265592 36-37 20.560733764243285 28.937286159627266 28.9411694718778 21.56081060425165 38-39 21.34739182236157 29.368348459869466 28.271658524574722 21.012601193194243 40-41 22.381425564718203 28.076253826158947 29.08646444460724 20.45585616451561 42-43 20.695684792254095 29.549018625000894 29.068617757563775 20.686678825181236 44-45 20.50285905658018 29.52589400256354 28.599544247487824 21.371702693368455 46-47 21.657588174476704 28.489674534815634 28.393225309466512 21.45951198124115 48-49 21.172921653929752 28.733024944669612 28.442492197561165 21.651561203839474 50-51 20.462213178911583 29.731511720714465 28.604112123587345 21.202162976786614 52-53 20.704553053408414 29.241328520603282 28.857569666801254 21.19654875918705 54-55 20.66983945996414 28.534869053422458 29.215532365521213 21.57975912109219 56-57 21.132818460084287 28.81570706756348 27.93144228221671 22.120032190135518 58-59 21.47854293918411 29.0197596976639 27.588692250957468 21.91300511219452 60-61 20.854211845683444 28.15171666952177 29.536101809841348 21.457969674953443 62-63 21.190578158458244 28.17557509450071 29.273008944001873 21.36083780303917 64-65 21.321300396620916 28.8934246195802 28.175783402732584 21.6094915810663 66-67 21.36443981507748 29.628171986980732 27.53421578958095 21.473172408360842 68-69 21.826903400773745 28.18935974435052 29.00447243376246 20.97926442111328 70-71 22.363496254123604 28.135109335745206 28.463537951475736 21.03785645865545 72-73 21.641669370292867 28.960766482159094 28.153176352258352 21.244387795289686 74-75 20.96735102836026 29.45469007079737 28.952421503495252 20.625537397347124 76-77 21.377962536278623 28.328117951178296 29.086987727097714 21.206931785445367 78-79 21.224067458552412 28.36852789837041 28.65005504128303 21.75734960179415 80-81 21.57212788142749 28.923117684028814 28.470891447526057 21.03386298701764 82-83 21.210994519265196 28.905230108237628 29.22176319920681 20.66201217329037 84-85 21.150869585813417 28.59621958487702 29.24809222115362 21.00481860815594 86-87 20.493092312005736 29.755690215214926 28.033161088683133 21.718056384096208 88-89 30.16524854104723 18.15279208290759 34.80577014670841 16.87618922933677 90-91 30.881705361674854 15.054357021443638 16.88856801266606 37.17536960421545 92-93 22.723984653529563 17.448739308042402 35.58512457812671 24.242151460301326 94-95 15.423346932921095 6.848833410362248 27.759494477823644 49.96832517889301 96-97 26.309916843557406 7.022491105634736 15.371769519554789 51.295822531253066 98-99 57.303272911568826 14.05617719494136 18.813500265285597 9.827049628204215 100-101 30.837415215858773 6.873931999297316 12.767014434692541 49.52163835015138 102-103 22.84854520059631 1.9653429959415165 37.5308618751793 37.65524992828288 104-105 41.07046060073786 4.445578458502569 41.52207074860624 12.961890192153328 106-107 5.395040817792248 1.6506667305068108 6.632591989657896 86.32170046204305 108-109 18.43805079519345 10.899193981696385 21.365645916036318 49.297109307073846 110-111 40.30390227668979 2.6686627793412283 24.982637256107708 32.04479768786127 112-113 27.05476363749152 2.667790930567705 39.07946680942061 31.19797862252016 114-115 27.828135348932147 3.8169465260912854 24.111371693028023 44.243546431948545 116-117 19.234836845755662 4.083980042073644 20.417522705174598 56.263660406996095 118-119 19.825908325714085 4.134211280151626 15.830118860291087 60.2097615338432 120-121 24.982176239102245 4.092588122358013 30.113033519898053 40.812202118641686 122-123 33.90992595637306 1.5454965832605456 34.101971261710254 30.44260619865614 124-125 38.48040175289903 1.8461465502552312 19.62515708981705 40.04829460702869 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11.0 1 28.0 2 75.5 3 121.5 4 143.0 5 142.0 6 133.0 7 131.5 8 118.5 9 105.0 10 113.5 11 117.0 12 121.5 13 120.5 14 141.0 15 195.0 16 258.0 17 337.0 18 511.0 19 767.5 20 1086.5 21 1632.5 22 2501.0 23 3732.5 24 5444.0 25 7778.0 26 10740.0 27 14412.5 28 19042.5 29 24665.0 30 31375.5 31 39425.5 32 48777.5 33 58657.5 34 68382.5 35 76116.5 36 80263.0 37 81587.0 38 81529.5 39 81019.0 40 80095.5 41 78460.0 42 76624.0 43 75702.5 44 74577.5 45 72510.0 46 69512.5 47 65924.5 48 62490.5 49 58577.5 50 54343.0 51 49447.5 52 43946.5 53 38332.5 54 32787.5 55 27951.5 56 23612.0 57 19575.5 58 15906.0 59 12754.5 60 10117.5 61 7853.0 62 5962.5 63 4490.5 64 3321.0 65 2450.0 66 1785.0 67 1286.5 68 953.0 69 670.0 70 464.5 71 318.0 72 231.0 73 156.5 74 101.0 75 77.5 76 61.5 77 37.5 78 22.5 79 14.5 80 6.5 81 7.0 82 8.5 83 6.5 84 7.0 85 7.5 86 7.0 87 8.0 88 7.0 89 5.5 90 3.5 91 5.0 92 5.0 93 3.0 94 2.5 95 1.0 96 3.0 97 5.0 98 4.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 4.957413067630681E-4 3 0.04478196471093049 4 0.04995970724823365 5 0.05254857851688523 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.885295927264835E-4 12-13 0.0020655887781794507 14-15 8.262355112717803E-5 16-17 3.0295302079965276E-4 18-19 0.006417095804210827 20-21 0.002754118370905934 22-23 8.537766949808397E-4 24-25 0.0 26-27 0.0 28-29 0.0015698474714163826 30-31 0.0 32-33 0.001294435634325789 34-35 8.262355112717803E-5 36-37 1.3770591854529672E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.2032946967247472E-4 46-47 0.0 48-49 2.7541183709059344E-4 50-51 1.1016473483623736E-4 52-53 0.0 54-55 0.0015423062877073233 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.754118370905934E-5 64-65 8.262355112717803E-5 66-67 0.0 68-69 4.682001230540088E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.1311775563589013E-4 80-81 0.0 82-83 7.160707764355429E-4 84-85 5.508236741811868E-5 86-87 0.001211812083198611 88-89 19.21903668650917 90-91 26.2614826080179 92-93 40.33943407274067 94-95 44.69994706584492 96-97 48.41271895929578 98-99 93.04425372480739 100-101 51.712703591315275 102-103 53.148921239375305 104-105 74.85002448411232 106-107 81.59323544462211 108-109 12.604856171676316 110-111 39.08903679116567 112-113 52.051047033181064 114-115 86.776871795239 116-117 66.40620051193552 118-119 71.92594946853778 120-121 80.37602528941653 122-123 75.87846736617601 124-125 50.0553577792552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1815463.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.64215822122122 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11513008883529 58.007900120916666 2 11.57217853161888 16.34967333589681 3 3.278167822758169 6.947305500329966 4 1.252470660362652 3.539089222271036 5 0.5936061609833876 2.096681017264009 6 0.33409621118110244 1.416076644681959 7 0.20191072427918044 0.9984386531763869 8 0.12779348955934633 0.7222086327274662 9 0.09088225463275952 0.5778106750141841 >10 0.395673799267021 4.855008313397723 >50 0.022543422059914917 1.091833357881983 >100 0.013359965002683886 1.8540055151327723 >500 0.001562049614014874 0.7283976160413815 >1k 6.248198456059478E-4 0.8155713952676623 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2837 0.1562686763652027 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2665 0.1467945091692863 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2267 0.12487172693687505 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.508236741811868E-5 0.0 6 0.0 0.0 0.0 5.508236741811868E-5 0.0 7 0.0 0.0 0.0 5.508236741811868E-5 0.0 8 0.0 0.0 0.0 5.508236741811868E-5 0.0 9 0.0 0.0 0.0 5.508236741811868E-5 0.0 10-11 0.0 0.0 0.0 5.508236741811868E-5 0.0 12-13 0.0 0.0 0.0 5.508236741811868E-5 0.0 14-15 0.0 0.0 0.0 5.508236741811868E-5 0.0 16-17 0.0 0.0 0.0 8.262355112717802E-5 0.0 18-19 0.0 0.0 0.0 1.3770591854529672E-4 0.0 20-21 0.0 0.0 0.0 2.7541183709059344E-4 0.0 22-23 0.0 0.0 0.0 4.4065893934494945E-4 0.0 24-25 0.0 0.0 0.0 5.783648578902462E-4 0.0 26-27 0.0 0.0 0.0 9.914826135261363E-4 0.0 28-29 0.0 0.0 0.0 0.0014046003691620265 0.0 30-31 0.0 0.0 0.0 0.0032223184939599433 0.0 32-33 0.0 0.0 0.0 0.007133166580646369 0.0 34-35 1.377059185452967E-4 0.0 0.0 0.013164685812930364 0.0 36-37 2.2032946967247472E-4 0.0 0.0 0.022941806029646433 0.0 38-39 2.2032946967247472E-4 0.0 0.0 0.03905339849944615 0.0 40-41 2.7541183709059344E-4 0.0 0.0 0.05998469811833125 0.0 42-43 2.7541183709059344E-4 0.0 0.0 0.08171469206477908 0.0 44-45 3.304942045087121E-4 0.0 0.0 0.10639159266809622 0.0 46-47 4.131177556358901E-4 0.0 0.0 0.13621869462500752 0.0 48-49 4.957413067630681E-4 0.0 0.0 0.16893762087137001 0.0 50-51 4.957413067630681E-4 0.0 0.0 0.2078257722685618 0.0 52-53 4.957413067630681E-4 0.0 0.0 0.24792573574895221 0.0 54-55 4.957413067630681E-4 0.0 0.0 0.2880256992293426 0.0 56-57 4.957413067630681E-4 0.0 0.0 0.33754474753823127 0.0 58-59 4.957413067630681E-4 0.0 0.0 0.3932054798142402 0.0 60-61 4.957413067630681E-4 0.0 0.0 0.45558626091525967 0.0 62-63 4.957413067630681E-4 0.0 0.0 0.5203631249989672 0.0 64-65 4.957413067630681E-4 0.0 0.0 0.587563613249072 0.0 66-67 4.957413067630681E-4 0.0 0.0 0.6645963040833109 0.0 68-69 4.957413067630681E-4 0.0 0.0 0.7642953891101057 0.0 70-71 4.957413067630681E-4 0.0 0.0 0.8689518872045312 0.0 72-73 4.957413067630681E-4 0.0 0.0 0.9729473968899394 0.0 74-75 4.957413067630681E-4 0.0 0.0 1.079421613109163 0.0 76-77 4.957413067630681E-4 0.0 0.0 1.1942958903596494 0.0 78-79 4.957413067630681E-4 0.0 0.0 1.2264915341155396 0.0 80-81 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 82-83 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 84-85 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 86-87 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 88-89 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 90-91 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 92-93 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 94-95 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 96-97 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 98-99 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 100-101 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 102-103 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 104-105 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 106-107 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 108-109 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 110-111 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 112-113 4.957413067630681E-4 0.0 0.0 1.2270698989734299 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 450 0.0 2649.8845 108-109 CCGCTCG 75 0.0 1987.4135 108-109 CCGCGCC 390 0.0 1465.0804 108-109 CGTCGGT 50 1.2671269E-5 1196.2699 110-111 CGACGCT 45 1.6130221E-5 1104.1185 108-109 CGTCGCG 55 1.6863354E-5 1087.5182 110-111 CCGTTCG 70 4.2526153E-8 1064.6858 108-109 CGGCGCG 100 1.4188117E-10 993.7067 108-109 CGACGCC 75 5.603397E-8 993.7067 108-109 CTCCGCC 325 0.0 920.20764 110-111 CGGCGCC 270 0.0 920.0988 108-109 CGTCTCG 110 2.2737368E-10 903.3698 108-109 CGCCGCC 320 0.0 841.1274 110-111 CCGCGCG 155 1.8189894E-12 801.37634 108-109 CGTCGCC 225 0.0 772.883 108-109 CCGTTCC 205 0.0 727.1025 108-109 CCGTGCG 110 2.5899317E-7 677.52734 108-109 CCGCGCT 75 7.4609685E-5 662.4711 108-109 CAGCGCG 120 3.666937E-7 621.0667 108-109 CCACGCG 120 3.666937E-7 621.0667 108-109 >>END_MODULE