FastQCFastQC Report
Fri 27 May 2016
SRR939915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815463
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34000.18728004922160352No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29750.16387004306890307No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA28070.15461620534265916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.13131636392479495No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC21770.11991431386924438No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA1655.456968E-1239.66366
GTACATG58200.030.0009021
TACATGG56150.029.9238262
GTATCAA92200.029.2144151
GTACGAC1454.025018E-628.7639033
ACATGGG54350.027.954933
TAGGACC23200.026.9661584
ATCAACG100700.026.3298453
TCAACGC100600.026.2967934
GTAGGAC64650.026.1738513
CAACGCA101450.026.0177355
AACGCAG102350.025.8094376
TATCAAC104150.025.6864972
GAGTACT47550.025.14947712-13
AGAGTAC75750.024.15159810-11
GACGTGG37100.024.0547437
TGTAGGA72900.024.0291042
CCTAACG2251.8876926E-723.8329492
AGTACTT49300.023.65334312-13
ACGCAGA111900.023.447257
Produced by FastQC (version 0.11.5)