FastQCFastQC Report
Fri 27 May 2016
SRR939914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459011
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC8150.01548.6053108-109
CCGCGCG2650.01307.4056108-109
CCGCGCC7150.01246.0189108-109
CGTCGCT1500.01219.1011110-111
CGTCGCG1500.01154.875108-109
CGTCGCC5000.01039.3875108-109
CGTCGTC1700.0896.39777110-111
CCGTTCG1850.0802.6158108-109
TTGTTCG4600.0699.3808108-109
CCGCGCT1803.6379788E-12687.42554108-109
CGGCGCG1501.0986696E-9659.9286108-109
GGTCGCC4000.0618.68304108-109
CTCCGCC5550.0604.059110-111
CGACGCC1702.05182E-9582.2899108-109
CGTCGTT1605.118927E-7571.4536110-111
CCTCGCC13200.0524.9432108-109
CGCCGCC7150.0511.51093110-111
CCGTTCC6500.0494.94644108-109
CCGCACC5500.0494.94644108-109
CCGCTCG4050.0488.836108-109
Produced by FastQC (version 0.11.5)