##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR939914_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2459011
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.003043500008744	34.0	31.0	34.0	30.0	34.0
2	32.13093516051779	34.0	31.0	34.0	30.0	34.0
3	32.22877286844182	34.0	31.0	34.0	30.0	34.0
4	35.53298826235426	37.0	35.0	37.0	35.0	37.0
5	35.52659870167315	37.0	35.0	37.0	35.0	37.0
6	35.67646993039072	37.0	37.0	37.0	35.0	37.0
7	35.671672879869185	37.0	37.0	37.0	35.0	37.0
8	35.68505468255327	37.0	37.0	37.0	35.0	37.0
9	37.41949995343656	39.0	39.0	39.0	35.0	39.0
10-11	37.3887605626815	39.0	39.0	39.0	35.0	39.0
12-13	37.336892962251895	39.0	39.0	39.0	35.0	39.0
14-15	38.88473963719561	41.0	40.0	41.0	36.0	41.0
16-17	38.86475579003103	41.0	40.0	41.0	36.0	41.0
18-19	38.87302029149117	41.0	40.0	41.0	36.0	41.0
20-21	38.90848942928682	41.0	40.0	41.0	36.0	41.0
22-23	38.88932298391508	41.0	40.0	41.0	36.0	41.0
24-25	38.892553144333235	41.0	39.5	41.0	35.5	41.0
26-27	38.851202170303424	41.0	39.0	41.0	35.0	41.0
28-29	38.79432503555291	41.0	39.0	41.0	35.0	41.0
30-31	38.71817145185605	41.0	39.0	41.0	35.0	41.0
32-33	38.61067599941603	41.0	39.0	41.0	34.5	41.0
34-35	38.535963238879376	41.0	39.0	41.0	34.0	41.0
36-37	38.70906982522648	41.0	39.0	41.0	35.0	41.0
38-39	38.68904205796558	41.0	39.0	41.0	35.0	41.0
40-41	38.63618930537521	41.0	39.0	41.0	35.0	41.0
42-43	38.53998131769236	41.0	39.0	41.0	34.5	41.0
44-45	38.431189409075444	41.0	39.0	41.0	34.0	41.0
46-47	38.34652854338594	41.0	39.0	41.0	34.0	41.0
48-49	38.241063175398565	41.0	38.5	41.0	33.5	41.0
50-51	38.11717109032859	40.5	38.0	41.0	33.0	41.0
52-53	37.98706858163709	40.0	38.0	41.0	33.0	41.0
54-55	37.824321648012145	40.0	38.0	41.0	33.0	41.0
56-57	37.64634440431539	40.0	37.0	41.0	33.0	41.0
58-59	37.46726834487524	40.0	37.0	41.0	33.0	41.0
60-61	37.22973484055175	40.0	36.5	41.0	32.0	41.0
62-63	36.96957719993932	39.5	36.0	41.0	32.0	41.0
64-65	36.66554216308914	39.0	35.0	41.0	31.5	41.0
66-67	36.348977902091534	39.0	35.0	41.0	31.0	41.0
68-69	35.980179836527775	38.0	35.0	40.5	31.0	41.0
70-71	35.589981297358975	37.0	35.0	40.0	31.0	41.0
72-73	35.18498778573988	37.0	35.0	39.0	30.0	41.0
74-75	34.76976821169161	36.0	35.0	39.0	30.0	41.0
76-77	34.349813807258286	36.0	35.0	38.5	30.0	40.0
78-79	33.92521607264059	35.0	34.0	37.0	29.5	39.0
80-81	33.535821311901415	35.0	34.0	37.0	29.0	39.0
82-83	33.36827895442517	35.0	34.0	36.5	30.0	39.0
84-85	33.01422340119666	35.0	34.0	36.0	30.0	37.0
86-87	32.454600447090314	35.0	34.0	36.0	30.0	37.0
88-89	16.951102292751028	18.0	17.0	18.5	2.0	19.0
90-91	2.8285418406017704	2.0	2.0	2.0	2.0	2.0
92-93	2.317734243563774	2.0	2.0	2.0	2.0	2.0
94-95	2.109034892483198	2.0	2.0	2.0	2.0	2.0
96-97	2.000031923403352	2.0	2.0	2.0	2.0	2.0
98-99	2.0	2.0	2.0	2.0	2.0	2.0
100-101	2.0	2.0	2.0	2.0	2.0	2.0
102-103	2.0	2.0	2.0	2.0	2.0	2.0
104-105	2.0	2.0	2.0	2.0	2.0	2.0
106-107	2.0	2.0	2.0	2.0	2.0	2.0
108-109	2.0	2.0	2.0	2.0	2.0	2.0
110-111	2.0	2.0	2.0	2.0	2.0	2.0
112-113	2.0	2.0	2.0	2.0	2.0	2.0
114-115	2.0	2.0	2.0	2.0	2.0	2.0
116-117	2.0	2.0	2.0	2.0	2.0	2.0
118-119	2.0	2.0	2.0	2.0	2.0	2.0
120-121	2.0	2.0	2.0	2.0	2.0	2.0
122-123	2.0	2.0	2.0	2.0	2.0	2.0
124-125	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.03670996012298389
1101	2	0.13870220922364496
1101	3	0.16601215585379236
1101	4	0.1479555034325486
1101	5	0.11122810968657149
1101	6	0.14027089978295493
1101	7	0.06593424428526617
1101	8	0.07094863682398511
1101	9	0.13148661952587304
1101	10-11	0.18689460684455383
1101	12-13	0.26015751265643416
1101	14-15	0.2662149926909336
1101	16-17	0.30283886399795534
1101	18-19	0.2618201754536216
1101	20-21	0.19235913525533022
1101	22-23	0.2381331431442888
1101	24-25	0.17565840171172198
1101	26-27	0.21421974844027147
1101	28-29	0.22721625310391147
1101	30-31	0.1998951837091738
1101	32-33	0.2685036277506683
1101	34-35	0.24769033536968266
1101	36-37	0.18888461387506794
1101	38-39	0.21676558748803387
1101	40-41	0.23603419173596052
1101	42-43	0.2490762152272623
1101	44-45	0.22350233581766332
1101	46-47	0.16180088584908958
1101	48-49	0.20826281085538767
1101	50-51	0.20341650081750373
1101	52-53	0.19157044909429288
1101	54-55	0.15019997148503705
1101	56-57	0.13175018236071878
1101	58-59	0.1196507359718808
1101	60-61	0.15725685733023909
1101	62-63	0.13850577094500238
1101	64-65	0.19427060969008636
1101	66-67	0.24603633208755582
1101	68-69	0.31286013377412303
1101	70-71	0.2952543241887895
1101	72-73	0.2608333557453264
1101	74-75	0.25530461622751943
1101	76-77	0.22702375460987412
1101	78-79	0.20479265677039393
1101	80-81	0.03468579505484115
1101	82-83	0.15433456888287367
1101	84-85	0.1610770229034415
1101	86-87	0.29261464869795617
1101	88-89	0.7863402143518137
1101	90-91	-0.10961773109821937
1101	92-93	-0.20782306789317406
1101	94-95	-0.023397204264522298
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
1101	102-103	0.0
1101	104-105	0.0
1101	106-107	0.0
1101	108-109	0.0
1101	110-111	0.0
1101	112-113	0.0
1101	114-115	0.0
1101	116-117	0.0
1101	118-119	0.0
1101	120-121	0.0
1101	122-123	0.0
1101	124-125	0.0
1102	1	-0.12374076489698993
1102	2	-0.02265179570429865
1102	3	0.060616802170478934
1102	4	0.04097014750800554
1102	5	0.04877273120089143
1102	6	0.07833978196406832
1102	7	0.1284243241654366
1102	8	0.12886665577964607
1102	9	0.1745533113445248
1102	10-11	0.13869950116750118
1102	12-13	0.10460429371942581
1102	14-15	0.12631771229749233
1102	16-17	0.13458328222646543
1102	18-19	0.09559184219116901
1102	20-21	0.1610797418044001
1102	22-23	0.16435628250378187
1102	24-25	0.23416351781919786
1102	26-27	0.2191199506215611
1102	28-29	0.20777151368311308
1102	30-31	0.24699136840825986
1102	32-33	0.30274451126381763
1102	34-35	0.23502763127177673
1102	36-37	0.2935020562222732
1102	38-39	0.2340744952142373
1102	40-41	0.17747971963505194
1102	42-43	0.23604797237976527
1102	44-45	0.22291984812427046
1102	46-47	0.16927651420741796
1102	48-49	0.18790779036808658
1102	50-51	0.12980079727977767
1102	52-53	0.16519112736827424
1102	54-55	0.20538136367541426
1102	56-57	0.11917257930170422
1102	58-59	0.18096623017930824
1102	60-61	0.1555953649980779
1102	62-63	0.16149405326685695
1102	64-65	0.10283343534723599
1102	66-67	0.11264095370450633
1102	68-69	0.07134395070487187
1102	70-71	0.11178287669621056
1102	72-73	0.15894358215931703
1102	74-75	0.10154165006016314
1102	76-77	-0.01611882802793474
1102	78-79	0.034320751723846854
1102	80-81	0.0544367590621917
1102	82-83	0.043040239047257955
1102	84-85	0.12074130474069023
1102	86-87	0.23220347804946329
1102	88-89	1.4519212665902828
1102	90-91	0.20604306911941617
1102	92-93	-0.020622003230039265
1102	94-95	0.10098763959268275
1102	96-97	0.0
1102	98-99	0.0
1102	100-101	0.0
1102	102-103	0.0
1102	104-105	0.0
1102	106-107	0.0
1102	108-109	0.0
1102	110-111	0.0
1102	112-113	0.0
1102	114-115	0.0
1102	116-117	0.0
1102	118-119	0.0
1102	120-121	0.0
1102	122-123	0.0
1102	124-125	0.0
1103	1	-0.028084876621356614
1103	2	0.09889251549780909
1103	3	0.040920521756966366
1103	4	0.10203726924202527
1103	5	0.0076108785829589465
1103	6	-0.002602476805691367
1103	7	0.07551904031998191
1103	8	0.1633352306711231
1103	9	0.15321037165708162
1103	10-11	0.0906188852273786
1103	12-13	0.160683293637355
1103	14-15	0.14747611403471694
1103	16-17	0.185851333594087
1103	18-19	0.18997288254753641
1103	20-21	0.15152791441576596
1103	22-23	0.14274143866665412
1103	24-25	0.17438012370968892
1103	26-27	0.21537300959585082
1103	28-29	0.19643549679904737
1103	30-31	0.16163438111129835
1103	32-33	0.2981839619846127
1103	34-35	0.24752125029876026
1103	36-37	0.1999275119384194
1103	38-39	0.2575881230367614
1103	40-41	0.2032585139730898
1103	42-43	0.23840366138808378
1103	44-45	0.2937230406097697
1103	46-47	0.270468035982681
1103	48-49	0.2113742842101658
1103	50-51	0.20575198835967257
1103	52-53	0.200669211119461
1103	54-55	0.18856842034770693
1103	56-57	0.21592121841322864
1103	58-59	0.22893860630006202
1103	60-61	0.17672123587200872
1103	62-63	0.17323782116566377
1103	64-65	0.1966454591414788
1103	66-67	0.2505952968693421
1103	68-69	0.24495001583814258
1103	70-71	0.19753410607246735
1103	72-73	0.2420643566716123
1103	74-75	0.3032380946009354
1103	76-77	0.3009492279689212
1103	78-79	0.24027844476800908
1103	80-81	0.22779574955333715
1103	82-83	0.3003689207056155
1103	84-85	0.2847271982136874
1103	86-87	0.30280412067077833
1103	88-89	0.9913022964256726
1103	90-91	-0.5857361334325519
1103	92-93	-0.3499580664064066
1103	94-95	-0.12568682662550978
1103	96-97	0.0
1103	98-99	0.0
1103	100-101	0.0
1103	102-103	0.0
1103	104-105	0.0
1103	106-107	0.0
1103	108-109	0.0
1103	110-111	0.0
1103	112-113	0.0
1103	114-115	0.0
1103	116-117	0.0
1103	118-119	0.0
1103	120-121	0.0
1103	122-123	0.0
1103	124-125	0.0
1104	1	-0.09710903670091042
1104	2	0.03322255781092309
1104	3	0.03889598623728574
1104	4	0.0862363133917583
1104	5	-0.038698139083017224
1104	6	-0.027758182988279145
1104	7	0.07908633035255264
1104	8	0.12745654856743727
1104	9	0.16725426771090213
1104	10-11	0.13272479993143804
1104	12-13	0.17060363944816004
1104	14-15	0.10468365124894774
1104	16-17	0.1147249117414475
1104	18-19	0.17255002534669472
1104	20-21	0.06712816200330707
1104	22-23	0.015237506692344027
1104	24-25	0.07861163835692508
1104	26-27	0.09819713111668449
1104	28-29	0.15953216755835342
1104	30-31	0.09705908507167749
1104	32-33	0.10425148032537379
1104	34-35	0.10341535588875672
1104	36-37	0.12890093686561244
1104	38-39	0.08085790213590371
1104	40-41	0.1631448672318072
1104	42-43	0.043641355576873764
1104	44-45	0.028340852707053443
1104	46-47	0.001982074399343503
1104	48-49	0.05038413697674571
1104	50-51	-0.0176340757086777
1104	52-53	0.022989228166473197
1104	54-55	0.014067907922672873
1104	56-57	-0.05777991095126822
1104	58-59	-0.06097057777721915
1104	60-61	-0.10845882318370315
1104	62-63	-0.0717015503272691
1104	64-65	-0.08059596915452971
1104	66-67	-0.07526298970309853
1104	68-69	-0.10682743948956386
1104	70-71	-0.10370586105115365
1104	72-73	-0.10004074627001103
1104	74-75	-0.1080100916173663
1104	76-77	-0.15520180043261433
1104	78-79	-0.14044463262339235
1104	80-81	-0.134540502742432
1104	82-83	-0.048182996575789616
1104	84-85	-0.04208247393574993
1104	86-87	-0.06666569698708003
1104	88-89	0.4790469159627655
1104	90-91	-0.630726645959061
1104	92-93	-0.3499580664064066
1104	94-95	-0.12568682662550978
1104	96-97	0.0
1104	98-99	0.0
1104	100-101	0.0
1104	102-103	0.0
1104	104-105	0.0
1104	106-107	0.0
1104	108-109	0.0
1104	110-111	0.0
1104	112-113	0.0
1104	114-115	0.0
1104	116-117	0.0
1104	118-119	0.0
1104	120-121	0.0
1104	122-123	0.0
1104	124-125	0.0
1105	1	-0.11449663709062463
1105	2	-0.11357689495825696
1105	3	-0.06229104025038623
1105	4	-0.00532702841884003
1105	5	-0.05474817744251936
1105	6	0.005031344946694105
1105	7	0.04506921997603541
1105	8	-0.0900136541809573
1105	9	0.04218645219420836
1105	10-11	-0.028729511472398883
1105	12-13	0.027319964278596842
1105	14-15	-0.018309731148974606
1105	16-17	0.09477067642905013
1105	18-19	0.042938687878802284
1105	20-21	0.08298408874215824
1105	22-23	0.00642658843338495
1105	24-25	0.04729283247481675
1105	26-27	0.04871161123956114
1105	28-29	0.03638067969611569
1105	30-31	0.024604147678459753
1105	32-33	0.013985772149389675
1105	34-35	0.1148222081976229
1105	36-37	-0.024385193625711565
1105	38-39	0.02084473910097273
1105	40-41	0.0450726399803969
1105	42-43	0.037776705777929465
1105	44-45	0.0330152597821467
1105	46-47	0.09285914200776091
1105	48-49	0.07422817462396836
1105	50-51	0.013151737081280146
1105	52-53	-0.03295720032090799
1105	54-55	-0.07187223713742696
1105	56-57	-0.08335071442982667
1105	58-59	-0.0793845105123836
1105	60-61	-0.09391304202269168
1105	62-63	1.406548497797644E-4
1105	64-65	-0.06552609838446699
1105	66-67	-0.047103665206826406
1105	68-69	0.07034457894182822
1105	70-71	-0.03473936324996174
1105	72-73	0.004454022247266209
1105	74-75	-0.05728094734015343
1105	76-77	0.0401745462682257
1105	78-79	0.01623482315807223
1105	80-81	-0.051944929538542794
1105	82-83	0.0038048727226396295
1105	84-85	-0.041988497083238485
1105	86-87	0.05764787192116927
1105	88-89	0.9676792860464332
1105	90-91	-0.8284699463821923
1105	92-93	-0.3499580664064066
1105	94-95	-0.12568682662550978
1105	96-97	0.0
1105	98-99	0.0
1105	100-101	0.0
1105	102-103	0.0
1105	104-105	0.0
1105	106-107	0.0
1105	108-109	0.0
1105	110-111	0.0
1105	112-113	0.0
1105	114-115	0.0
1105	116-117	0.0
1105	118-119	0.0
1105	120-121	0.0
1105	122-123	0.0
1105	124-125	0.0
1106	1	-0.16462232236240837
1106	2	-0.0579321932532082
1106	3	-0.05232647804227497
1106	4	-0.10598652575610856
1106	5	-0.046217898005629365
1106	6	-0.09324855544535637
1106	7	-0.1847219113240186
1106	8	-0.03762085734386744
1106	9	-0.06737370510751362
1106	10-11	-0.22721855330188845
1106	12-13	-0.1774433960160664
1106	14-15	-0.18978457646463198
1106	16-17	-0.1840558682521305
1106	18-19	-0.2670036635523445
1106	20-21	-0.1756291797409375
1106	22-23	-0.20845378829996974
1106	24-25	-0.17605856733153047
1106	26-27	-0.2807330153647314
1106	28-29	-0.12488515678374768
1106	30-31	-0.11001323929143325
1106	32-33	-0.05497228624898298
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2215	108-109	0.0
2215	110-111	0.0
2215	112-113	0.0
2215	114-115	0.0
2215	116-117	0.0
2215	118-119	0.0
2215	120-121	0.0
2215	122-123	0.0
2215	124-125	0.0
2216	1	0.2012728552677885
2216	2	0.05142689663729527
2216	3	0.08457992822000193
2216	4	0.07269272915700498
2216	5	0.054497063152005865
2216	6	0.07000257471301552
2216	7	0.01915743412359916
2216	8	-0.027452995329610985
2216	9	-0.1380333170854371
2216	10-11	-0.05921862001401479
2216	12-13	-0.07490970151464893
2216	14-15	-0.14648778753557679
2216	16-17	-0.19493059489062858
2216	18-19	-0.1518240296280311
2216	20-21	-0.08152481039849135
2216	22-23	-0.09299807973826546
2216	24-25	-0.18457832053687895
2216	26-27	-0.11209656680590285
2216	28-29	-0.11432468091339842
2216	30-31	-0.15360834023226744
2216	32-33	-0.18914461915832703
2216	34-35	-0.12829522029686302
2216	36-37	-0.12036707361121302
2216	38-39	-0.10450233818954757
2216	40-41	-0.14839079133708566
2216	42-43	-0.15134344858601878
2216	44-45	-0.16471872464207848
2216	46-47	-0.12448223562714134
2216	48-49	-0.08384927760836547
2216	50-51	-0.05377144201194284
2216	52-53	-0.06615757892198104
2216	54-55	-0.11716137695393769
2216	56-57	-0.09913531003788023
2216	58-59	-0.059951004997145674
2216	60-61	-0.0710893486662485
2216	62-63	-0.08238303382028533
2216	64-65	-0.0601913975028765
2216	66-67	-0.012632926053306903
2216	68-69	-0.049463223707746806
2216	70-71	0.006691102675574712
2216	72-73	0.07610737765757136
2216	74-75	0.03888049620028511
2216	76-77	0.01961867747807844
2216	78-79	0.03207493292129726
2216	80-81	0.05381281409422911
2216	82-83	0.07215642586847082
2216	84-85	-0.02905506477748787
2216	86-87	-0.08546786292112074
2216	88-89	-1.1958917911333575
2216	90-91	-0.8284699463821923
2216	92-93	-0.3499580664064066
2216	94-95	-0.12568682662550978
2216	96-97	0.0
2216	98-99	0.0
2216	100-101	0.0
2216	102-103	0.0
2216	104-105	0.0
2216	106-107	0.0
2216	108-109	0.0
2216	110-111	0.0
2216	112-113	0.0
2216	114-115	0.0
2216	116-117	0.0
2216	118-119	0.0
2216	120-121	0.0
2216	122-123	0.0
2216	124-125	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19397.0
3	3396.0
4	2408.0
5	2141.0
6	2368.0
7	2657.0
8	3117.0
9	3718.0
10	4441.0
11	5550.0
12	6766.0
13	7547.0
14	8255.0
15	8937.0
16	9609.0
17	10886.0
18	13749.0
19	17158.0
20	20578.0
21	27992.0
22	33469.0
23	45765.0
24	69500.0
25	118907.0
26	251415.0
27	776299.0
28	947074.0
29	34653.0
30	1259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.066586932714	19.44314197862474	14.471671741200018	24.018599347461237
2	14.444136277421896	20.181343188894274	41.381155794425666	23.993364739258165
3	18.672292019268326	24.823712732717095	29.421421364405674	27.08257388360891
4	12.174184053892297	17.37979348121952	39.00970424254951	31.436318222338677
5	13.479231360141657	37.96925147615048	35.22693982774447	13.324577335963387
6	31.167123693224635	37.33590455675066	18.483040539468917	13.013931210555787
7	27.821957689493864	32.2193760011647	22.142723233039625	17.815943076301814
8	25.088297693666274	36.01622766225934	20.8623304247114	18.03314421936299
9	25.43922147530917	17.467029414276478	20.881614958865562	36.21213415154879
10-11	24.24728826948435	26.84804854020498	28.77036021866679	20.134302971643876
12-13	25.217530457924187	24.08294787321175	28.384064684098924	22.31545698476514
14-15	21.80645869679261	26.19700611221589	26.022708325708315	25.973826865283183
16-17	21.969124860054954	28.927560419861564	27.113287062510345	21.990027657573137
18-19	22.035502693865265	28.138533508349	28.241326404556645	21.584637393229087
20-21	22.779994615605474	27.54984021687235	27.805354951162244	21.86481021635994
22-23	22.23104695489789	27.669440967418435	27.684650414518437	22.41486166316524
24-25	22.253743476544024	27.522406365811296	27.976450695015192	22.247399462629488
26-27	21.902850373585153	27.918439567777455	27.660368335074548	22.518341723562845
28-29	22.081888458583826	28.20300620704772	27.39700095039445	22.318104383974
30-31	22.335320988804035	27.719782465389542	27.433691837897435	22.511204707908995
32-33	21.888662914709048	27.801310541797157	27.540043993797436	22.76998254969636
34-35	22.468502555297682	27.960308400660594	27.36299460473711	22.208194439304616
36-37	22.51848203890266	27.391929025066602	27.478732286055074	22.61085664997566
38-39	22.149941582205205	27.939159279889353	27.350629989048443	22.560269148857
40-41	22.80986136296259	27.578872156326263	27.45353721475829	22.15772926595286
42-43	22.260941492331675	27.659127999020743	27.37706338035901	22.702867128288567
44-45	22.369598785200694	27.78961182298383	27.40842136683629	22.432368024979187
46-47	22.761833924289075	27.75388154018018	26.958927796581634	22.525356738949114
48-49	22.350993714129324	27.737761624916075	27.227840343683447	22.683404317271158
50-51	22.33655915858787	28.026676600207644	27.295081067210408	22.341683173994078
52-53	22.603213243047712	27.730579489071012	27.250894770295865	22.41531249758541
54-55	22.256706087126265	27.683187201093144	27.47836483716693	22.58174187461366
56-57	22.334324653285407	27.79650030032399	27.213379688012786	22.65579535837782
58-59	22.4693179493707	27.597395863621593	27.175823938973842	22.757462248033864
60-61	22.134488214977484	27.67807870725263	27.65449198885243	22.532941088917454
62-63	22.228168606844097	27.684794351223797	27.726010116670913	22.3610269252612
64-65	22.05925681021436	27.87873649074413	27.33611941560999	22.72588728343151
66-67	22.183898323350324	27.976857362573814	27.124970160767887	22.714274153307976
68-69	22.574246030963355	27.51559727344837	27.549330450322664	22.360826245265613
70-71	22.515962718344895	27.406485778225477	27.244591423137187	22.83296008029244
72-73	22.373059738244358	27.577794487295908	27.35628266811332	22.692863106346415
74-75	22.256061481628183	28.102720971967997	27.200122325601633	22.441095220802186
76-77	22.48259564515978	27.39196774638259	27.393248749192256	22.73218785926537
78-79	22.501468074065762	27.551968195253036	27.317239012217954	22.62932471846325
80-81	22.755835577799367	27.703841097091477	27.110269128523623	22.430054196585537
82-83	22.613393932640747	27.73805060118081	27.425463421220226	22.22309204495822
84-85	22.515167695604205	27.80904827920516	27.307077769947163	22.368706255243474
86-87	22.483251704008346	27.94933593441024	27.13237167502073	22.435040686560676
88-89	31.748429765397372	17.617049269576263	33.1040236316527	17.53049733337366
90-91	31.176904435781267	14.501365581391243	16.304065411100403	38.01766457172709
92-93	23.236871195222626	17.151955144969392	34.02329357418408	25.587880085623897
94-95	15.387916790847244	6.750327228867905	27.11628570091816	50.7454702793667
96-97	26.54001829560882	6.921641688144177	15.064684668642256	51.473655347604755
98-99	57.36303069583851	14.167557120418541	18.889881525858193	9.57953065788475
100-101	31.28794228694941	6.762712163642492	12.347757952905116	49.60158759650298
102-103	23.376658810567534	1.9277325496289792	36.7992848957616	37.89632374404188
104-105	42.30918970020583	4.414960650952949	39.58369250518624	13.692157143654981
106-107	5.324201014619851	1.5584516501448664	6.574714430025643	86.54263290520964
108-109	19.05703930410123	10.410150989266489	20.133530391143005	50.399279315489274
110-111	40.6862363457933	2.5969682156164264	23.925514884426043	32.79128055416424
112-113	27.56556478662995	2.6830299963296094	38.233304597481926	31.51810061955852
114-115	27.70203944714024	3.6789814667386005	23.348292061568333	45.27068702455283
116-117	19.047708528567775	3.985752785614096	20.054603006802342	56.91193567901579
118-119	19.621061497999154	4.075972316272951	15.548334382614954	60.75463180311294
120-121	25.110845034650414	4.085379444038419	29.624054694262597	41.17972082704857
122-123	33.90125630339794	1.5385677064047236	33.65762029475328	30.90255569544405
124-125	38.41136722860823	1.7264161293075075	18.845155234833065	41.0170614072512
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	28.5
2	56.5
3	90.5
4	122.0
5	150.5
6	164.5
7	155.5
8	145.0
9	144.5
10	155.0
11	155.0
12	134.5
13	124.5
14	139.0
15	189.5
16	261.0
17	325.5
18	446.0
19	677.5
20	1065.5
21	1692.0
22	2764.5
23	4372.0
24	6638.0
25	9979.5
26	14114.0
27	18900.0
28	24644.0
29	30727.5
30	36964.0
31	43853.0
32	51102.0
33	58559.0
34	65837.0
35	72246.0
36	77370.5
37	81778.5
38	85899.5
39	89028.5
40	91698.0
41	93820.5
42	95458.0
43	97346.5
44	98686.5
45	98639.0
46	97412.5
47	95453.5
48	93237.5
49	90819.5
50	88081.5
51	84837.0
52	80263.0
53	73854.0
54	66170.0
55	58876.0
56	52124.5
57	45479.0
58	38726.0
59	31910.5
60	25681.5
61	20304.5
62	15976.0
63	12207.5
64	8872.0
65	6375.0
66	4577.0
67	3204.5
68	2260.5
69	1636.5
70	1166.5
71	787.0
72	519.0
73	348.5
74	241.0
75	185.5
76	138.0
77	95.0
78	62.5
79	38.0
80	30.0
81	28.5
82	19.0
83	13.5
84	12.5
85	9.5
86	10.0
87	11.5
88	14.0
89	12.5
90	8.5
91	8.0
92	6.5
93	7.0
94	6.5
95	5.0
96	7.5
97	12.5
98	10.5
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0010980024082852822
3	0.04436743064589788
4	0.049613442152149786
5	0.05152477967768342
6	0.0
7	0.0
8	0.0
9	4.0666755862417856E-5
10-11	7.523349834547304E-4
12-13	0.0018300040138088038
14-15	4.0666755862417856E-5
16-17	4.066675586241786E-4
18-19	0.0074623497007536765
20-21	0.0021553380607081465
22-23	5.286678262114321E-4
24-25	0.0
26-27	0.0
28-29	0.0013013361875973714
30-31	0.0
32-33	0.0011386691641477
34-35	8.133351172483571E-5
36-37	8.133351172483571E-5
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.6266702344967142E-4
46-47	0.0
48-49	1.6266702344967142E-4
50-51	8.133351172483571E-5
52-53	0.0
54-55	0.0020740045489833106
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.0333377931208928E-5
64-65	1.0166688965604465E-4
66-67	0.0
68-69	3.050006689681339E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	2.84667291036925E-4
80-81	0.0
82-83	6.913348496611036E-4
84-85	8.133351172483571E-5
86-87	0.0011996692979413268
88-89	19.326103055252702
90-91	26.280077640970294
92-93	40.36574053552424
94-95	44.80583860747268
96-97	48.38750212992134
98-99	93.03537072424646
100-101	51.62807323757397
102-103	53.04953902198892
104-105	74.87849790017206
106-107	81.68308315822907
108-109	12.581806262761736
110-111	39.147100195973096
112-113	52.02498077479116
114-115	86.84652081670232
116-117	66.45549775905842
118-119	71.80445715777604
120-121	80.38571197932828
122-123	75.83890433999684
124-125	49.95732023972239
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2459011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.16471492975417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.10372532500153	32.80196515932979
2	15.475620716469635	14.907577203395158
3	6.312397911060691	9.121045377282417
4	3.2651949129896587	6.290687286969216
5	1.9383555789331621	4.6680171945907185
6	1.2249179252943054	3.5398693610487677
7	0.7964232906032758	2.685165052872648
8	0.5586863535738605	2.152717516402307
9	0.42009910896684793	1.8210558443068767
>10	1.7961838470065261	14.443376043973075
>50	0.07138248430559078	2.3686669632884376
>100	0.032372083008325676	2.80947457848201
>500	0.0024467894693057405	0.7904817720598232
>1k	0.0021936733173058953	1.599900645998587
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6266702344967142E-4
2	0.0	0.0	0.0	0.0	1.6266702344967142E-4
3	0.0	0.0	0.0	0.0	1.6266702344967142E-4
4	0.0	0.0	0.0	0.0	1.6266702344967142E-4
5	0.0	0.0	0.0	0.0	2.033337793120893E-4
6	0.0	0.0	0.0	0.0	2.8466729103692503E-4
7	0.0	0.0	0.0	0.0	2.8466729103692503E-4
8	0.0	0.0	0.0	0.0	2.8466729103692503E-4
9	0.0	0.0	0.0	0.0	2.8466729103692503E-4
10-11	0.0	0.0	0.0	0.0	3.2533404689934285E-4
12-13	0.0	0.0	0.0	0.0	0.0011386691641477
14-15	0.0	0.0	0.0	0.0	0.0013013361875973714
16-17	0.0	0.0	0.0	0.0	0.001443669833115834
18-19	0.0	0.0	0.0	0.0	0.001667336990359132
20-21	0.0	0.0	0.0	6.1000133793626784E-5	0.0019113375255336395
22-23	0.0	0.0	0.0	2.033337793120893E-4	0.001992671037258475
24-25	0.0	0.0	0.0	4.4733431448659646E-4	0.002033337793120893
26-27	0.0	0.0	0.0	8.133351172483572E-4	0.0020536711710521017
28-29	0.0	0.0	0.0	0.0017486705020839678	0.0020740045489833106
30-31	0.0	0.0	0.0	0.004392009633141129	0.00209433792691452
32-33	0.0	0.0	0.0	0.010959690704921613	0.002216338194501773
34-35	0.0	0.0	0.0	0.019987710506378376	0.0024400053517450713
36-37	0.0	0.0	0.0	0.0350547435534042	0.0024400053517450713
38-39	0.0	0.0	0.0	0.058865129110849845	0.0024400053517450713
40-41	0.0	0.0	0.0	0.08666085674281246	0.0024400053517450713
42-43	0.0	0.0	0.0	0.11518858598029857	0.0024806721076074895
44-45	0.0	0.0	0.0	0.15180899963440586	0.002582338997263534
46-47	0.0	0.0	0.0	0.19320775710234722	0.0027450060207132054
48-49	0.0	0.0	0.0	0.23962885891929725	0.0029686731779565035
50-51	0.0	0.0	0.0	0.28891696702454767	0.0030500066896813395
52-53	0.0	0.0	0.0	0.338388075531179	0.003334673980718264
54-55	0.0	0.0	0.0	0.3953418671164952	0.003436340870374309
56-57	0.0	0.0	0.0	0.45955467462325306	0.003517674382099145
58-59	0.0	0.0	0.0	0.5314128322321454	0.0035786745158927716
60-61	0.0	0.0	0.0	0.6097370040231622	0.003680341405548816
62-63	0.0	0.0	0.0	0.6914975166845532	0.0038633418069296964
64-65	0.0	0.0	0.0	0.7806390455349732	0.003944675318654532
66-67	0.0	0.0	0.0	0.8840952724489642	0.003944675318654532
68-69	0.0	0.0	0.0	0.9967625195657929	0.003944675318654532
70-71	0.0	0.0	0.0	1.1182544527047664	0.00398534207451695
72-73	0.0	0.0	0.0	1.248042404039673	0.004310676121416293
74-75	0.0	0.0	0.0	1.3856993726339573	0.004615676790384427
76-77	0.0	0.0	0.0	1.5354750344752421	0.004636010168315636
78-79	0.0	0.0	0.0	1.5774227931473264	0.004636010168315636
80-81	0.0	0.0	0.0	1.578358128532162	0.004636010168315636
82-83	0.0	0.0	0.0	1.578358128532162	0.004636010168315636
84-85	0.0	0.0	0.0	1.578358128532162	0.004636010168315636
86-87	0.0	2.0333377931208928E-5	0.0	1.578358128532162	0.004636010168315636
88-89	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
90-91	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
92-93	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
94-95	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
96-97	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
98-99	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
100-101	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
102-103	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
104-105	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
106-107	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
108-109	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
110-111	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
112-113	0.0	4.0666755862417856E-5	0.0	1.578358128532162	0.004636010168315636
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTCC	815	0.0	1548.6053	108-109
CCGCGCG	265	0.0	1307.4056	108-109
CCGCGCC	715	0.0	1246.0189	108-109
CGTCGCT	150	0.0	1219.1011	110-111
CGTCGCG	150	0.0	1154.875	108-109
CGTCGCC	500	0.0	1039.3875	108-109
CGTCGTC	170	0.0	896.39777	110-111
CCGTTCG	185	0.0	802.6158	108-109
TTGTTCG	460	0.0	699.3808	108-109
CCGCGCT	180	3.6379788E-12	687.42554	108-109
CGGCGCG	150	1.0986696E-9	659.9286	108-109
GGTCGCC	400	0.0	618.68304	108-109
CTCCGCC	555	0.0	604.059	110-111
CGACGCC	170	2.05182E-9	582.2899	108-109
CGTCGTT	160	5.118927E-7	571.4536	110-111
CCTCGCC	1320	0.0	524.9432	108-109
CGCCGCC	715	0.0	511.51093	110-111
CCGTTCC	650	0.0	494.94644	108-109
CCGCACC	550	0.0	494.94644	108-109
CCGCTCG	405	0.0	488.836	108-109
>>END_MODULE