FastQCFastQC Report
Fri 27 May 2016
SRR939914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459011
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.18141439790224606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29960.12183760056380391No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92400.040.177171
GGTATCA61200.036.0257951
ATCAACG106450.034.700723
CAACGCA107200.034.5135235
TCAACGC107200.034.4579474
TATCAAC110350.033.528322
AACGCAG111650.033.144056
ACGCAGA123400.029.9399037
CGCAGAG125050.029.354558
TACATGG71550.025.97992
ACATGGG65550.025.6311993
GTACATG75450.025.5887471
GAGTACT76050.025.03367812-13
GCAGAGT145700.025.0308069
AGAGTAC105200.024.96829610-11
GTACTTT78750.023.57171614-15
AGTACTT83150.022.2879312-13
CATGGGG41550.021.221854
TAAGGTG17650.020.9285685
CAGAGTA136550.020.41229410-11
Produced by FastQC (version 0.11.5)