##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939914_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2459011 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.87691067669075 34.0 31.0 34.0 31.0 34.0 2 32.95867281602238 34.0 33.0 34.0 31.0 34.0 3 32.977009456240744 34.0 33.0 34.0 31.0 34.0 4 36.39366558343985 37.0 37.0 37.0 35.0 37.0 5 36.28063111551758 37.0 37.0 37.0 35.0 37.0 6 36.39951346293286 37.0 37.0 37.0 35.0 37.0 7 36.38863307240187 37.0 37.0 37.0 35.0 37.0 8 36.41345850018564 37.0 37.0 37.0 35.0 37.0 9 38.25960640273671 39.0 39.0 39.0 37.0 39.0 10-11 38.253718669822945 39.0 39.0 39.0 37.0 39.0 12-13 38.24336023710345 39.0 39.0 39.0 37.0 39.0 14-15 39.82913496523602 41.0 40.0 41.0 38.0 41.0 16-17 39.840125969342964 41.0 40.0 41.0 38.0 41.0 18-19 39.86208235750064 41.0 40.0 41.0 38.0 41.0 20-21 39.867338332362074 41.0 40.0 41.0 38.0 41.0 22-23 39.82067465334641 41.0 40.0 41.0 38.0 41.0 24-25 39.783991816222056 41.0 40.0 41.0 37.0 41.0 26-27 39.69876364928827 41.0 40.0 41.0 37.0 41.0 28-29 39.6246289666862 41.0 40.0 41.0 37.0 41.0 30-31 39.513262039088076 41.0 40.0 41.0 36.5 41.0 32-33 39.376025971416965 41.0 39.5 41.0 36.0 41.0 34-35 39.281162020015365 41.0 39.0 41.0 35.5 41.0 36-37 39.26816370483906 41.0 39.5 41.0 35.5 41.0 38-39 39.327740502177505 41.0 40.0 41.0 35.5 41.0 40-41 39.276471516394196 41.0 40.0 41.0 35.0 41.0 42-43 39.16882885029794 41.0 39.5 41.0 35.0 41.0 44-45 39.032907132176305 41.0 39.0 41.0 35.0 41.0 46-47 38.878229296249586 41.0 39.0 41.0 35.0 41.0 48-49 38.69376977166837 41.0 39.0 41.0 35.0 41.0 50-51 38.544215133645196 41.0 39.0 41.0 35.0 41.0 52-53 38.4130922960491 41.0 39.0 41.0 35.0 41.0 54-55 38.27430641831208 41.0 38.0 41.0 35.0 41.0 56-57 38.11973024927501 40.5 38.0 41.0 34.0 41.0 58-59 37.937773153515785 40.0 37.0 41.0 34.0 41.0 60-61 37.733433888665 40.0 37.0 41.0 34.0 41.0 62-63 37.49240812668182 40.0 36.5 41.0 34.0 41.0 64-65 37.22894712549069 39.0 36.0 41.0 33.5 41.0 66-67 36.93385165824797 39.0 35.0 41.0 33.0 41.0 68-69 36.59739769362561 38.5 35.0 41.0 33.0 41.0 70-71 36.234654908009766 37.0 35.0 40.0 33.0 41.0 72-73 35.87385599332414 37.0 35.0 39.0 32.0 41.0 74-75 35.501778153900084 36.5 35.0 39.0 32.0 41.0 76-77 35.12079815828396 36.0 35.0 39.0 32.0 40.5 78-79 34.75467799859374 35.5 35.0 37.0 32.0 39.0 80-81 34.4808213546015 35.0 35.0 37.0 31.5 39.0 82-83 34.25890652786832 35.0 35.0 36.5 32.0 39.0 84-85 34.01102211417517 35.0 35.0 36.0 32.0 37.0 86-87 33.81402604543046 35.0 35.0 36.0 32.0 37.0 88-89 33.63481029568391 35.0 35.0 35.5 31.0 37.0 90-91 33.50012749027963 35.0 35.0 35.0 31.0 36.0 92-93 33.380411677702945 35.0 35.0 35.0 31.0 36.0 94-95 33.29482706665403 35.0 35.0 35.0 31.0 36.0 96-97 33.23477711161114 35.0 35.0 35.0 31.0 36.0 98-99 33.16772474787628 35.0 35.0 35.0 31.0 36.0 100-101 33.12424181917039 35.0 34.5 35.0 31.0 35.0 102-103 32.31065131469522 34.5 33.0 35.0 29.0 35.0 104-105 32.98604804939872 35.0 34.0 35.0 31.0 35.0 106-107 33.061565808367675 35.0 34.0 35.0 31.0 35.0 108-109 33.060370002411545 35.0 35.0 35.0 31.0 35.0 110-111 33.02838275225284 35.0 34.5 35.0 31.0 35.0 112-113 32.97273944687518 35.0 34.0 35.0 31.0 35.0 114-115 32.93179188706354 35.0 34.0 35.0 31.0 35.0 116-117 32.879477155653234 35.0 34.0 35.0 31.0 35.0 118-119 32.81370904806852 35.0 34.0 35.0 31.0 35.0 120-121 32.72856912799496 35.0 34.0 35.0 31.0 35.0 122-123 32.58958764316223 35.0 34.0 35.0 30.5 35.0 124-125 30.667838411458916 33.5 31.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10074345573003285 1101 2 0.11143764635195197 1101 3 0.08313123050052695 1101 4 0.06389898550659012 1101 5 0.0207998060849377 1101 6 0.02337391174992831 1101 7 0.06307518942006851 1101 8 0.1028899865443762 1101 9 0.11030897050696353 1101 10-11 0.10391473696554243 1101 12-13 0.06895092743462783 1101 14-15 0.12851921100784836 1101 16-17 0.13268589350352045 1101 18-19 0.17299005047846094 1101 20-21 0.18365517179730517 1101 22-23 0.16446084287643004 1101 24-25 0.23750563954724413 1101 26-27 0.25837515640117914 1101 28-29 0.27648865347222085 1101 30-31 0.28297422803371575 1101 32-33 0.26880897895851774 1101 34-35 0.2302752301252724 1101 36-37 0.29515420931973324 1101 38-39 0.23803068646049041 1101 40-41 0.2715066279489591 1101 42-43 0.24999268013556986 1101 44-45 0.24869773908766746 1101 46-47 0.3340783730047221 1101 48-49 0.27654961794791433 1101 50-51 0.3293768366861016 1101 52-53 0.2763938663263943 1101 54-55 0.31095128719444176 1101 56-57 0.3131675750264975 1101 58-59 0.3380882745765277 1101 60-61 0.3556582913520998 1101 62-63 0.3359849292886423 1101 64-65 0.2930085803311968 1101 66-67 0.31899776651234646 1101 68-69 0.2700729219193647 1101 70-71 0.2656361403226981 1101 72-73 0.2580333793852816 1101 74-75 0.2838978358367683 1101 76-77 0.30127173871886015 1101 78-79 0.30375207054362363 1101 80-81 0.3216541408901108 1101 82-83 0.28542298598908644 1101 84-85 0.2878029217776401 1101 86-87 0.2765285423012287 1101 88-89 0.3020635018301121 1101 90-91 0.3323556986712859 1101 92-93 0.36251167212500945 1101 94-95 0.3229111715765498 1101 96-97 0.35146845439327024 1101 98-99 0.34954992493389625 1101 100-101 0.3875402667667416 1101 102-103 0.3699500750457503 1101 104-105 0.3469364698073747 1101 106-107 0.3580476891469857 1101 108-109 0.3947123148442131 1101 110-111 0.3612857568720713 1101 112-113 0.32947637271984576 1101 114-115 0.3826487695587488 1101 116-117 0.4091039708224784 1101 118-119 0.4090120693549295 1101 120-121 0.41417417418089286 1101 122-123 0.4020717121581612 1101 124-125 0.5755218710010723 1102 1 0.10168882956015324 1102 2 0.16936010082419983 1102 3 0.14266708238975667 1102 4 0.04842851243613211 1102 5 -0.015712322835177872 1102 6 0.030458214482806056 1102 7 0.07427913038525702 1102 8 0.03728878314524309 1102 9 0.005902823994198059 1102 10-11 0.14494078675447497 1102 12-13 0.1383325825032884 1102 14-15 0.13101697215910946 1102 16-17 0.17290937570236053 1102 18-19 0.04462224317956043 1102 20-21 0.07122065328524485 1102 22-23 0.14605862379617207 1102 24-25 0.1313883403106857 1102 26-27 0.12498769237125629 1102 28-29 0.11162924238880834 1102 30-31 0.10628959926427939 1102 32-33 0.07712986892839524 1102 34-35 0.13861867165302755 1102 36-37 0.16158067102031026 1102 38-39 0.09980420428696135 1102 40-41 0.11079290288645893 1102 42-43 0.03215254537240497 1102 44-45 -0.0032406903759181205 1102 46-47 0.022911185947599222 1102 48-49 0.005171232463780484 1102 50-51 7.531087770402678E-4 1102 52-53 -0.009247314122859507 1102 54-55 -0.03775811228270243 1102 56-57 -0.0677027115957145 1102 58-59 -7.925001471420501E-5 1102 60-61 -0.019808448926745825 1102 62-63 -0.05471433164123596 1102 64-65 0.02812622632078643 1102 66-67 0.02589904765477513 1102 68-69 -0.05576260547269385 1102 70-71 -0.022094255411481356 1102 72-73 -0.03660750771108212 1102 74-75 0.021217889755263286 1102 76-77 0.030462795291093414 1102 78-79 0.07694919177159676 1102 80-81 0.021071096582865323 1102 82-83 -0.07092585156202347 1102 84-85 -0.03412895964586937 1102 86-87 -0.00909215649788564 1102 88-89 -0.08542648890487214 1102 90-91 0.017417017948723412 1102 92-93 0.005027559754068989 1102 94-95 -0.01886103646796755 1102 96-97 -0.03513699866560671 1102 98-99 -0.10481269698519213 1102 100-101 -0.04200037808953283 1102 102-103 -0.0438086104132438 1102 104-105 -0.030440064761776853 1102 106-107 -0.011152622869857964 1102 108-109 -0.07098257362298455 1102 110-111 -0.044316926772012266 1102 112-113 -0.05481043578420497 1102 114-115 -0.07808803758420169 1102 116-117 -9.458400288337998E-4 1102 118-119 -0.018019856014980462 1102 120-121 0.03617597152179286 1102 122-123 0.06874816305260367 1102 124-125 0.07805315943949864 1103 1 0.06346704314021423 1103 2 0.06599124165590098 1103 3 0.0923704118583828 1103 4 0.05619046900132929 1103 5 -0.0337814709095241 1103 6 0.06580339724271056 1103 7 0.0033953472099810256 1103 8 -0.018716064921321163 1103 9 0.07666027126831665 1103 10-11 0.09270211206100498 1103 12-13 0.0559816489769247 1103 14-15 0.04854214359844633 1103 16-17 0.07370694998572702 1103 18-19 0.051614779597926486 1103 20-21 0.06830920414680719 1103 22-23 0.045659584151025 1103 24-25 0.10883510942361596 1103 26-27 0.0697122847359779 1103 28-29 0.07697537203670635 1103 30-31 -0.016067352261366352 1103 32-33 0.02788761078988955 1103 34-35 0.037542123122911164 1103 36-37 0.09245612158118632 1103 38-39 0.003586748301472653 1103 40-41 0.055978440329568 1103 42-43 0.14047013581598833 1103 44-45 0.02344450723960989 1103 46-47 0.0859456463582049 1103 48-49 0.1059926683247383 1103 50-51 0.12775696576749596 1103 52-53 0.11129619958913395 1103 54-55 0.12549089190675033 1103 56-57 0.08571692181133272 1103 58-59 0.06027833697799423 1103 60-61 0.07941611292712736 1103 62-63 -0.02174213437710648 1103 64-65 0.00832785033892236 1103 66-67 0.05030451772154265 1103 68-69 0.04185325679507912 1103 70-71 0.07613960909095141 1103 72-73 0.07551413162559584 1103 74-75 0.010457412439535574 1103 76-77 0.028525967450057976 1103 78-79 0.02033391657438699 1103 80-81 -0.14349315608052393 1103 82-83 -0.0034153884496248565 1103 84-85 0.020933797986572245 1103 86-87 -0.006514432774551437 1103 88-89 -0.01769378065790761 1103 90-91 -0.006220041985081082 1103 92-93 0.04280624467392613 1103 94-95 0.01354761514248537 1103 96-97 0.009566846330926637 1103 98-99 -0.01661744447120128 1103 100-101 0.032231222298477746 1103 102-103 0.005552408522419228 1103 104-105 -0.01291985922395611 1103 106-107 0.04003567948620912 1103 108-109 -0.0035385189223120506 1103 110-111 0.05926815953156961 1103 112-113 -0.016351885280350587 1103 114-115 0.060916888337594344 1103 116-117 0.10797573870701171 1103 118-119 0.0750424849867386 1103 120-121 0.07036073251673969 1103 122-123 0.07062813552972358 1103 124-125 0.08021509412105843 1104 1 0.06971517862336185 1104 2 0.08128809303234874 1104 3 0.08758750757532141 1104 4 0.05482873751205375 1104 5 0.01270004627447463 1104 6 0.013703672227237007 1104 7 -0.01830833920618602 1104 8 -0.05598001962497534 1104 9 -0.04121006997631582 1104 10-11 0.012869943944998852 1104 12-13 0.018655188565574576 1104 14-15 0.018541636246915516 1104 16-17 0.058730541831572225 1104 18-19 0.06685013996971634 1104 20-21 0.1091574958942303 1104 22-23 0.08389501555203083 1104 24-25 0.08478380603755653 1104 26-27 0.09344760959315579 1104 28-29 0.07500725400645791 1104 30-31 0.06279780385069245 1104 32-33 0.09119533978299899 1104 34-35 0.07753481726097533 1104 36-37 0.06564482590686538 1104 38-39 0.06671528073645305 1104 40-41 0.05251911470121229 1104 42-43 0.04538474855456798 1104 44-45 0.03738551240444821 1104 46-47 0.0926857098786158 1104 48-49 0.044042206127500094 1104 50-51 0.08820343510824102 1104 52-53 0.09657062602823885 1104 54-55 0.034957868396077174 1104 56-57 0.06055533035107885 1104 58-59 0.012874454724133955 1104 60-61 0.040752070959015896 1104 62-63 0.12502512318496883 1104 64-65 0.07761887731987116 1104 66-67 0.056454835830948014 1104 68-69 0.009218783273752251 1104 70-71 0.06939122500555328 1104 72-73 0.060332144634088536 1104 74-75 0.018817753785640434 1104 76-77 0.04286940384339033 1104 78-79 0.025740254409612362 1104 80-81 0.05571517260209191 1104 82-83 0.044933791162961256 1104 84-85 -0.027352672977698944 1104 86-87 0.0023759894433865725 1104 88-89 0.02983024243673782 1104 90-91 0.003048255647620124 1104 92-93 -0.04581670182078312 1104 94-95 -0.04484265994351233 1104 96-97 -0.05505147311788505 1104 98-99 0.00790285466325713 1104 100-101 0.043831307533537256 1104 102-103 -1.3682852236485132E-4 1104 104-105 0.006509471782322862 1104 106-107 0.01214879858190443 1104 108-109 -0.01711707215871172 1104 110-111 -0.08968903014687157 1104 112-113 -0.025770156023419588 1104 114-115 -0.058879359290536115 1104 116-117 -0.06917416337415006 1104 118-119 0.011777373521610457 1104 120-121 -0.06628323862510399 1104 122-123 0.010271162997739225 1104 124-125 0.07280735599562504 1105 1 -0.025194539273499572 1105 2 0.037330459491869306 1105 3 -0.03348213413596568 1105 4 0.03755425450240324 1105 5 -0.021760120248117687 1105 6 0.006706318954279311 1105 7 -0.03478472497253904 1105 8 0.016719390940906464 1105 9 0.03238133624059003 1105 10-11 0.07369246104989458 1105 12-13 0.04225608660146918 1105 14-15 -0.013725723976243387 1105 16-17 0.04404877663564122 1105 18-19 0.027983886759365362 1105 20-21 0.07000025175366176 1105 22-23 0.0536223885459961 1105 24-25 -0.04879911245360802 1105 26-27 0.04358265229065239 1105 28-29 0.04391397688142007 1105 30-31 -0.026977036082719508 1105 32-33 -0.033261547747571285 1105 34-35 0.009831437388960751 1105 36-37 0.05754999086840229 1105 38-39 0.02889728518898238 1105 40-41 -0.015473412648560725 1105 42-43 -0.03240608156383473 1105 44-45 -0.05874433567938553 1105 46-47 0.03248988816165621 1105 48-49 -0.05484215213924415 1105 50-51 -0.18818252735436403 1105 52-53 -0.09488909022128666 1105 54-55 -0.18104290663839606 1105 56-57 -0.029738500280352298 1105 58-59 -0.031133383478795906 1105 60-61 0.030952552296220404 1105 62-63 0.029932668161279707 1105 64-65 -0.028496638901721383 1105 66-67 -0.02224375734027717 1105 68-69 0.022179038438949306 1105 70-71 0.09768265051987868 1105 72-73 0.021210788982685358 1105 74-75 -0.032125113308495656 1105 76-77 -0.011115120569407111 1105 78-79 -0.011244735965213692 1105 80-81 -0.06431821349843858 1105 82-83 -0.03861068938351764 1105 84-85 -0.0884267625083055 1105 86-87 -0.10026740370148701 1105 88-89 -0.09552354354391923 1105 90-91 -0.13915111648031342 1105 92-93 -0.19020809580030118 1105 94-95 -0.10801887360200624 1105 96-97 -0.13003550960412724 1105 98-99 -0.11541626970467433 1105 100-101 0.021162568878615673 1105 102-103 -0.04542633684614117 1105 104-105 -0.10716102246688308 1105 106-107 -0.12385649487219297 1105 108-109 -0.07341756118350418 1105 110-111 -0.06502646038984494 1105 112-113 -0.11969456819742419 1105 114-115 -0.06239870612787968 1105 116-117 -0.14053079533161394 1105 118-119 -0.09240647909005162 1105 120-121 -0.2855893239008651 1105 122-123 -0.1527264711075489 1105 124-125 -0.12725555672200528 1106 1 -0.05091805457949761 1106 2 -0.001597468194425744 1106 3 -0.047778852636824354 1106 4 -0.027583595464250266 1106 5 -0.13596118473802932 1106 6 -0.02357153575589166 1106 7 -0.062227390579195685 1106 8 -0.08068051665984655 1106 9 -0.07115750052346215 1106 10-11 -0.02911190570338107 1106 12-13 -0.01846823787598595 1106 14-15 -0.15407421468312776 1106 16-17 -0.11115094857757413 1106 18-19 -0.1105363209792074 1106 20-21 0.0011859079314362475 1106 22-23 -0.055557141736755966 1106 24-25 0.009618621329885002 1106 26-27 -0.013932825185300146 1106 28-29 -0.0062580812981494205 1106 30-31 -0.1306176973551132 1106 32-33 -0.0654939960351868 1106 34-35 -0.06816594764615758 1106 36-37 -0.037013032437243965 1106 38-39 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-0.10981250208417492 2216 46-47 -0.11773964116815705 2216 48-49 -0.041356674205161426 2216 50-51 -0.13147307259867347 2216 52-53 -0.08317970102632444 2216 54-55 -0.17497738573047172 2216 56-57 -0.13692571064458292 2216 58-59 -0.24103990116234542 2216 60-61 -0.21434325286117684 2216 62-63 -0.19188383633970574 2216 64-65 -0.10521510704369774 2216 66-67 -0.06659452885651973 2216 68-69 -0.08379387186741383 2216 70-71 -0.09357960964015177 2216 72-73 -0.016268944860172496 2216 74-75 -0.08334938102549216 2216 76-77 -0.11781976146779272 2216 78-79 -0.053795228047050614 2216 80-81 -0.05339106093934731 2216 82-83 0.014219603753076626 2216 84-85 0.01583152224331741 2216 86-87 -0.03160471025787359 2216 88-89 -0.04799981480897486 2216 90-91 0.021399826756471896 2216 92-93 0.013585581911407019 2216 94-95 -0.0429024389596151 2216 96-97 -0.010676919363788784 2216 98-99 0.007265217572054894 2216 100-101 -0.010634445874245557 2216 102-103 0.07109271498058689 2216 104-105 -0.03189906485748395 2216 106-107 -0.021713302467716744 2216 108-109 -0.013132132832758714 2216 110-111 -0.0412865558316966 2216 112-113 -0.015986629351509407 2216 114-115 -0.10289180537541398 2216 116-117 -0.2179450570728676 2216 118-119 -0.0833164231716026 2216 120-121 -0.09961644880304021 2216 122-123 -0.11918945636925571 2216 124-125 -0.2418793874645928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 219.0 3 14.0 4 40.0 5 75.0 6 162.0 7 385.0 8 854.0 9 1429.0 10 2785.0 11 4128.0 12 5313.0 13 7658.0 14 11928.0 15 12678.0 16 5487.0 17 4080.0 18 3739.0 19 3575.0 20 3543.0 21 3592.0 22 3893.0 23 3975.0 24 4758.0 25 6524.0 26 8549.0 27 11151.0 28 14344.0 29 18569.0 30 23479.0 31 30537.0 32 40100.0 33 53478.0 34 75649.0 35 124776.0 36 299821.0 37 968751.0 38 695090.0 39 3883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12264553836893 19.37082846951351 14.644537811708148 23.861988180409416 2 14.549141911117925 20.446553512774038 40.897458368425355 24.106846207682683 3 18.535378654263848 25.1502738296006 29.50731005270005 26.807037463435503 4 12.197302086082576 17.533715790616633 38.703080222089284 31.5659019012115 5 13.169026445239657 37.78284661500362 35.12072968450357 13.92739725525315 6 30.909093127277593 37.66225527254656 18.117161736974744 13.311489863201102 7 27.289711188766542 32.69391637532325 22.0683030698114 17.94806936609881 8 25.23709735336686 35.590446728379824 20.66741466386283 18.505041254390484 9 25.588336123750565 16.952262515295782 20.957775300720492 36.501626060233164 10-11 24.136146605281557 26.77118971814278 28.64184015443607 20.45082352213959 12-13 25.458629505927384 24.035516717900002 28.493691162829283 22.01216261334333 14-15 21.899454699470642 25.77999854413014 26.69931122715596 25.621235529243258 16-17 21.495577184399988 28.732939529945074 27.688088359118435 22.083394926536506 18-19 21.573551317989224 27.373932040157605 29.66320606129863 21.38931058055454 20-21 22.291157716394437 26.80187205584934 29.41637341234966 21.49059681540656 22-23 21.707839891077718 26.926036223653426 29.348768056621367 22.017355828647485 24-25 21.809052501188486 26.748355334726032 29.659708720294457 21.78288344379102 26-27 21.418997312334103 27.13995586030319 29.380104440362402 22.060942387000303 28-29 21.550005266344886 27.424867151875286 29.133114898632012 21.89201268314782 30-31 21.86761262963037 26.97159549103278 29.127136885520233 22.03365499381662 32-33 21.510131512221783 26.95364111831952 29.23128444728389 22.30494292217481 34-35 21.99902725119977 27.134343847994174 29.086470129657815 21.78015877114824 36-37 22.075602752488706 26.655879131894896 29.099442824777928 22.16907529083847 38-39 21.789491791618662 27.181923952353205 28.85224994926822 22.176334306759912 40-41 22.421717511633744 26.878692287265082 28.886410024192656 21.813180176908524 42-43 22.002829592872907 26.94558910065876 28.759834746570878 22.291746559897454 44-45 22.084813772691543 27.134770848930728 28.670022216248725 22.110393162129004 46-47 22.574665180432298 27.083246069253047 28.09265594989205 22.249432800422607 48-49 22.29990024444787 27.229870057514994 28.074742243934654 22.395487454102483 50-51 22.292946229195394 27.5568104412709 28.035722491684666 22.11452083784904 52-53 22.585096203311007 27.296238203082456 27.91799223346296 22.20067336014357 54-55 22.255248146510933 27.241073748755092 28.11831667284124 22.385361431892743 56-57 22.25770848524061 27.348657651389114 27.83503205150363 22.55860181186664 58-59 22.393230449152117 27.134364181372106 27.875637807232255 22.59676756224352 60-61 22.085342440517753 27.302663550508722 28.2810446964247 22.330949312548825 62-63 22.145041238123785 27.227897719855665 28.353553522127388 22.27350751989316 64-65 21.93056476770539 27.373118705040362 28.0985526294921 22.59776389776215 66-67 22.01112561106884 27.50536699510494 27.870574796127386 22.61293259769883 68-69 22.451302576523652 27.116186141501604 28.17120378884031 22.261307493134435 70-71 22.346382346398613 27.001790557260623 27.925169915872683 22.72665718046808 72-73 22.21376805553127 27.175742605462116 27.975556026386215 22.634933312620397 74-75 22.076477087739747 27.650445646644116 27.820168352235918 22.452908913380217 76-77 22.27722852805457 27.047804991518948 27.998004075622273 22.676962404804208 78-79 22.335849656630245 27.162424242917172 27.85087175291204 22.650854347540538 80-81 22.483510647166685 27.38670140149841 27.57777415391798 22.55201379741693 82-83 22.484872983488078 27.314456909708824 27.856097431040368 22.344572675762738 84-85 22.312649272410738 27.376961713469356 27.7769599241321 22.533429089987806 86-87 22.317996950806645 27.461487565529392 27.5596774475592 22.660838036104757 88-89 22.351425024125554 27.4135414603676 27.458478225595574 22.77655528991127 90-91 22.25746028144087 27.479809791747744 27.67009124481647 22.592638681994917 92-93 22.327614638568107 27.371309034404483 27.848635081339612 22.452441245687798 94-95 22.019715822292042 27.31473639883375 27.77472209621066 22.89082568266355 96-97 22.214906724695417 27.04865899339206 27.91233955439809 22.824094727514435 98-99 22.065802160817285 27.098748788622263 27.838446638581814 22.99700241197864 100-101 22.003717350420974 26.99507667619828 27.9483466818652 23.05285929151555 102-103 21.93700743532596 26.815140029227276 27.979594994641122 23.26825754080564 104-105 21.92669101840533 26.94671572801857 27.77314167522719 23.353451578348913 106-107 21.948224846355615 26.917085206927567 27.583435057985596 23.551254888731226 108-109 21.83660094969326 26.854546219821884 27.572682359873678 23.736170470611174 110-111 21.831521197615995 27.02342728513127 27.744546816951587 23.400504700301152 112-113 21.937087069072454 26.95049810984649 27.621985566100467 23.49042925498059 114-115 21.705549309470374 27.12006128009432 27.456495830306032 23.717893580129275 116-117 21.749070088099913 26.997508509153935 27.51673007737591 23.73669132537024 118-119 21.555810626848434 27.066773891498908 27.483059730399358 23.8943557512533 120-121 21.389863710664088 27.25990053259531 27.590929877289373 23.759305879451233 122-123 21.4061260285248 27.172839340491578 27.568734716750786 23.852299914232837 124-125 21.417421332711395 27.10606168127876 27.43823199827408 24.038284987735768 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 61.0 2 93.0 3 127.5 4 76.5 5 67.0 6 180.0 7 439.5 8 769.0 9 812.0 10 594.0 11 463.0 12 497.5 13 608.0 14 722.5 15 846.5 16 979.0 17 1145.5 18 1387.0 19 1648.5 20 1968.0 21 2417.0 22 3039.5 23 3945.0 24 5276.5 25 7075.5 26 9700.5 27 13779.0 28 18493.5 29 23917.5 30 30210.0 31 37783.0 32 47330.0 33 53869.0 34 58149.5 35 64700.0 36 72299.0 37 78518.5 38 80934.5 39 81529.5 40 83050.5 41 85287.5 42 86895.5 43 87200.0 44 89207.5 45 93682.0 46 94722.5 47 95705.5 48 98343.5 49 94247.5 50 87995.5 51 84957.0 52 82276.0 53 79529.0 54 77272.0 55 71664.0 56 64067.5 57 57504.5 58 51444.5 59 44303.0 60 35385.5 61 29024.5 62 24217.5 63 16802.5 64 10266.5 65 7402.0 66 5626.5 67 4288.5 68 3048.0 69 2196.0 70 1635.0 71 1010.0 72 676.0 73 533.0 74 354.5 75 251.5 76 172.0 77 99.0 78 60.5 79 29.0 80 20.5 81 15.5 82 10.0 83 4.0 84 1.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 1.0 97 3.5 98 8.0 99 8.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015494033983581203 2 0.0 3 0.0 4 0.0 5 0.14168297742466382 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0333377931208928E-5 18-19 0.0 20-21 0.004473343144865964 22-23 4.066675586241786E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.946686289731929E-4 92-93 0.0 94-95 8.74335251041984E-4 96-97 0.0 98-99 0.0013826696993222071 100-101 6.1000133793626784E-5 102-103 2.6433391310571604E-4 104-105 0.001992671037258475 106-107 0.0068116816069549915 108-109 2.6433391310571604E-4 110-111 0.00512401123866465 112-113 0.008784019266282257 114-115 0.005591678931082456 116-117 0.0016876703682903412 118-119 1.423336455184625E-4 120-121 5.896679600050589E-4 122-123 0.0011386691641477 124-125 0.0012810028096661625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2459011.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.90326976289024 #Duplication Level Percentage of deduplicated Percentage of total 1 66.93675968960763 31.395528967754238 2 15.946630058432387 14.958981828793366 3 6.657032310829273 9.367097468853057 4 3.3970510709013224 6.373312111071996 5 1.942222178954588 4.554828539948776 6 1.2272739094714697 3.4537895549338367 7 0.8529190982499303 2.8003286185796292 8 0.6020320107242959 2.2589815843917505 9 0.4337207556034204 1.8308629441648634 >10 1.8860984865837558 14.745819626163078 >50 0.07727162722643177 2.4977977896022963 >100 0.03579701668206346 3.0366378295629315 >500 0.0029420124823813054 0.9448662673215149 >1k 0.002249774251229595 1.7811668688585005 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4461 0.18141439790224606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2996 0.12183760056380391 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.133351172483571E-5 2 0.0 0.0 0.0 0.0 8.133351172483571E-5 3 0.0 0.0 0.0 4.0666755862417856E-5 8.133351172483571E-5 4 0.0 0.0 0.0 4.0666755862417856E-5 8.133351172483571E-5 5 0.0 0.0 0.0 4.0666755862417856E-5 1.2200026758725358E-4 6 0.0 0.0 0.0 4.0666755862417856E-5 2.4400053517450716E-4 7 0.0 0.0 0.0 4.0666755862417856E-5 2.4400053517450716E-4 8 0.0 0.0 0.0 4.0666755862417856E-5 2.4400053517450716E-4 9 0.0 0.0 0.0 4.0666755862417856E-5 2.4400053517450716E-4 10-11 0.0 0.0 0.0 4.0666755862417856E-5 3.2533404689934285E-4 12-13 0.0 0.0 0.0 4.0666755862417856E-5 0.001159002542078909 14-15 0.0 0.0 0.0 4.0666755862417856E-5 0.0015453367227718786 16-17 0.0 0.0 0.0 4.0666755862417856E-5 0.0019316709034648484 18-19 0.0 0.0 0.0 4.0666755862417856E-5 0.002033337793120893 20-21 0.0 0.0 0.0 4.0666755862417856E-5 0.002236671572432982 22-23 0.0 0.0 0.0 1.6266702344967142E-4 0.002236671572432982 24-25 0.0 0.0 0.0 4.066675586241786E-4 0.002236671572432982 26-27 0.0 0.0 0.0 7.116682275923126E-4 0.002318005084157818 28-29 0.0 0.0 0.0 0.0016266702344967143 0.0023586718400202358 30-31 0.0 0.0 0.0 0.004270009365553875 0.0023586718400202358 32-33 0.0 0.0 0.0 0.010858023815265568 0.0023993385958826535 34-35 0.0 0.0 0.0 0.019865710238791124 0.0025213388634699073 36-37 0.0 0.0 0.0 0.0348310763961609 0.0025213388634699073 38-39 0.0 0.0 0.0 0.0585397950639505 0.002562005619332325 40-41 0.0 0.0 0.0 0.08639652282970675 0.002562005619332325 42-43 0.0 0.0 0.0 0.11496491882305529 0.002562005619332325 44-45 0.0 0.0 0.0 0.15152433234336893 0.0027043392648507876 46-47 0.0 0.0 0.0 0.1928620896775167 0.0029483398000252946 48-49 0.0 0.0 0.0 0.2393238582503291 0.003131340201406175 50-51 0.0 0.0 0.0 0.2885712995997171 0.0032533404689934287 52-53 0.0 0.0 0.0 0.338001741350486 0.0035176743820991446 54-55 0.0 0.0 0.0 0.39493519955787104 0.003721008161411234 56-57 0.0 0.0 0.0 0.4592293405763537 0.003782008295204861 58-59 0.0 0.0 0.0 0.5308231642721404 0.003782008295204861 60-61 0.0 0.0 0.0 0.6091270026852259 0.0038836751848609057 62-63 0.0 0.0 0.0 0.690887515346617 0.00418867585382904 64-65 0.0 0.0 0.0 0.7802730447322115 0.0042496759876226664 66-67 0.0 0.0 0.0 0.8839529388034457 0.004290342743485084 68-69 0.0 0.0 0.0 0.996579519164412 0.004310676121416293 70-71 0.0 0.0 0.0 1.1179697854137294 0.004392009633141129 72-73 0.0 0.0 0.0 1.247473069457599 0.004636010168315635 74-75 0.0 0.0 0.0 1.3851300380518834 0.004839343947627725 76-77 0.0 0.0 0.0 1.5350073667828243 0.004880010703490143 78-79 0.0 0.0 0.0 1.7066210765222278 0.004920677459352561 80-81 0.0 0.0 0.0 1.8918988162314037 0.004920677459352561 82-83 0.0 0.0 0.0 2.0876279121972208 0.005063011104871023 84-85 0.0 0.0 0.0 2.2926493618776007 0.0052053447503894856 86-87 0.0 0.0 0.0 2.5085288353732453 0.005490012041426411 88-89 0.0 0.0 0.0 2.744294352485613 0.005774679332463336 90-91 0.0 0.0 0.0 3.005700259169235 0.005815346088325754 92-93 0.0 0.0 0.0 3.2762968526777634 0.006364347292468394 94-95 0.0 0.0 0.0 3.5507161212373592 0.006405014048330813 96-97 0.0 0.0 0.0 3.851609447863389 0.007279349299372797 98-99 0.0 0.0 0.0 4.169115144259217 0.007889350637309065 100-101 0.0 0.0 0.0 4.520658915311888 0.007889350637309065 102-103 0.0 0.0 0.0 4.88635471740468 0.008153684550414781 104-105 0.0 0.0 0.0 5.2667718851196685 0.008296018195933242 106-107 0.0 0.0 0.0 5.66020241471063 0.00833668495179566 108-109 0.0 0.0 0.0 6.078358331865941 0.008397685085589288 110-111 0.0 0.0 0.0 6.5317316595981065 0.008418018463520497 112-113 0.0 0.0 0.0 7.0119857129553305 0.00854001873110775 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9240 0.0 40.17717 1 GGTATCA 6120 0.0 36.025795 1 ATCAACG 10645 0.0 34.70072 3 CAACGCA 10720 0.0 34.513523 5 TCAACGC 10720 0.0 34.457947 4 TATCAAC 11035 0.0 33.52832 2 AACGCAG 11165 0.0 33.14405 6 ACGCAGA 12340 0.0 29.939903 7 CGCAGAG 12505 0.0 29.35455 8 TACATGG 7155 0.0 25.9799 2 ACATGGG 6555 0.0 25.631199 3 GTACATG 7545 0.0 25.588747 1 GAGTACT 7605 0.0 25.033678 12-13 GCAGAGT 14570 0.0 25.030806 9 AGAGTAC 10520 0.0 24.968296 10-11 GTACTTT 7875 0.0 23.571716 14-15 AGTACTT 8315 0.0 22.28793 12-13 CATGGGG 4155 0.0 21.22185 4 TAAGGTG 1765 0.0 20.928568 5 CAGAGTA 13655 0.0 20.412294 10-11 >>END_MODULE