FastQCFastQC Report
Fri 27 May 2016
SRR939913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551282
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA66020.25877186449792694No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC57480.22529849699092458No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA45810.17955678752877965No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA40710.15956683737822788No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37090.14537789236940488No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA30300.11876382148268988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCT450.04057.8997110-111
CCGCGCC2500.02880.4631108-109
CCGCTCC3950.02766.0496108-109
CCGCTCG1200.02483.158108-109
CCGCGCG1000.02234.842108-109
CGGCTCG302.6026046E-62028.9498110-111
CGCGTTA150.00198655092028.9498110-111
CGTCGCC1500.01820.9824108-109
CGCCGCT354.132333E-61739.1110-111
CCTCGCG1900.01699.0029108-109
CTCCGCC2700.01465.3525110-111
CCGCGCT357.612376E-61418.9474108-109
CCGCACG357.612376E-61418.9474108-109
CGCGGCT458.78056E-61352.6333110-111
CGTCGCG753.45608E-111324.351108-109
CGGATCG250.0055169921217.37110-111
CGCCGTC504.441972E-81217.37110-111
CGCCGCC1250.01217.3699110-111
CGGCGCG853.6379788E-121168.5449108-109
TTGTTCG3400.01168.5449108-109
Produced by FastQC (version 0.11.5)