FastQCFastQC Report
Fri 27 May 2016
SRR939913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551282
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA67910.26617990484783727No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC56720.22231960245868548No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA44770.17548040553729458No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA42500.16658291792126467No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38990.1528251287000026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33710.13212965089707843No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA28840.11304120830233585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC48200.032.6345834
GTACATG90350.031.7259641
TACATGG90450.030.6971862
GTATCAA126750.029.7144051
ACATGGG86200.028.9619583
CGTATAC1653.3692413E-728.8886493
CATGGGG44250.027.7379994
ATCAACG139550.026.5998613
TCAACGC139400.026.5857414
CAACGCA139550.026.514475
TATCAAC143300.026.1948262
AACGCAG143000.025.8362456
AGAGTAC108000.025.20235810-11
AGTACTT66600.024.16389312-13
GAGTACT63400.024.1636112-13
CTACAGT166000.023.7254564
ACGCAGA155800.023.7136297
CGCAGAG156200.023.6148138
TACAGTG166700.023.5900865
GTCCTAC162350.023.5290071
Produced by FastQC (version 0.11.5)