FastQCFastQC Report
Fri 27 May 2016
SRR939912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336865
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA18940.1416747390349811No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14910.1115295860090585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG600.02942.5906108-109
CCGCTCC3300.02063.6348108-109
CGTCGTC200.00357859541510.6702110-111
CCGCTCG1400.01441.2688108-109
CCGTTCC1600.01418.7489108-109
CCGCGCC3400.01409.4761108-109
CGTCGCT501.2263086E-51208.5361110-111
CGTCGCC1150.01096.6174108-109
TTGTTCG2600.0873.07623108-109
CGTCTCT2200.0824.00195110-111
CGCCGCC2750.0769.0684110-111
CGGCGCG804.6079134E-5755.3351110-111
CCTCGCG1701.8189894E-12741.8295108-109
CCTCTCG2750.0733.7368108-109
CGACGCC757.116498E-5672.59204108-109
CTCCGCC1901.3005774E-9636.0717110-111
CTCCGCT1101.3038455E-4549.33466110-111
TGTCGTG1706.704631E-7533.17773110-111
CAGCTCG2652.910383E-11475.89062108-109
CCGCGCG1902.1564665E-6398.24533108-109
Produced by FastQC (version 0.11.5)