FastQCFastQC Report
Fri 27 May 2016
SRR939912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336865
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43510.32546293006399296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.22231115333261023No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA18310.13696222131628846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.13382054283716005No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14360.10741548323877131No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53400.061.590721
GGTATCA26250.059.0148771
ATCAACG67300.047.6250953
TCAACGC67950.047.0818444
TATCAAC70000.046.4689942
CAACGCA69500.046.0318225
AACGCAG72000.044.6215636
ACGCAGA83850.038.3154757
CGCAGAG84350.037.9472858
GTCGTAC650.001925894136.6660461
GCAGAGT93950.034.069759
GTACATG64250.031.6226831
TACATGG63700.031.424512
ACATGGG62600.029.5023123
GGACCGT1055.9088215E-428.331156
AGAGTAC88550.027.9840110-11
AGTACTT60350.026.81483512-13
GAGTACT57150.026.49446112-13
CATGGGG34250.025.3957444
CAGAGTA94850.024.65123710-11
Produced by FastQC (version 0.11.5)