FastQCFastQC Report
Fri 27 May 2016
SRR939911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158528
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCC6150.01770.712108-109
CCGCTCC8350.01748.783108-109
CGTATCG250.00538061471232.5364110-111
CCGCGCG2650.0933.9519108-109
CGTCGCC3300.0824.9909108-109
CCGCGAC901.1807242E-7824.9908108-109
CGACGCC1701.8189894E-12727.93304108-109
TGTCGCG855.6916455E-5725.02136110-111
CCGCTCG2750.0719.992108-109
CGTCGCG1459.258656E-10682.7511108-109
CCTCGCC11200.0662.9391108-109
CTCCGCC5600.0660.2873110-111
CGCGTGT1051.0723549E-4586.92206110-111
CCTCGCG4900.0555.6061108-109
CGTCGTC1756.9998714E-7528.22986110-111
CCGTTCG1152.718928E-4430.43002108-109
CGTCGCT1452.8213309E-4425.01254110-111
TTTTTCG11000.0419.9066292-93
CGGCGCC4250.0407.64252108-109
GGTCGCC4000.0371.2459108-109
Produced by FastQC (version 0.11.5)