##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939911_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158528 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96220757849794 34.0 31.0 34.0 30.0 34.0 2 32.06115927150354 34.0 31.0 34.0 30.0 34.0 3 32.151876185993416 34.0 31.0 34.0 30.0 34.0 4 35.431934169952854 37.0 35.0 37.0 33.0 37.0 5 35.44799140896018 37.0 35.0 37.0 33.0 37.0 6 35.473134469416195 37.0 37.0 37.0 35.0 37.0 7 35.46926238621876 37.0 37.0 37.0 35.0 37.0 8 35.46213762341744 37.0 37.0 37.0 35.0 37.0 9 37.157416998991906 39.0 39.0 39.0 35.0 39.0 10-11 37.13348888687105 39.0 39.0 39.0 35.0 39.0 12-13 37.083485365953095 39.0 38.0 39.0 35.0 39.0 14-15 38.60497640058411 41.0 40.0 41.0 35.0 41.0 16-17 38.58736161865865 41.0 39.5 41.0 35.0 41.0 18-19 38.5884531032259 41.0 39.0 41.0 35.0 41.0 20-21 38.628708082545145 41.0 39.0 41.0 35.0 41.0 22-23 38.60268571915676 41.0 39.0 41.0 34.0 41.0 24-25 38.60980700736798 41.0 39.0 41.0 34.0 41.0 26-27 38.573621004684675 41.0 39.0 41.0 34.0 41.0 28-29 38.51479920575503 41.0 39.0 41.0 34.0 41.0 30-31 38.4452731213123 41.0 39.0 41.0 34.0 41.0 32-33 38.338635635025355 41.0 39.0 41.0 34.0 41.0 34-35 38.2546978774424 40.0 38.5 41.0 33.5 41.0 36-37 38.42176612951049 41.0 39.0 41.0 34.0 41.0 38-39 38.401450664526934 41.0 39.0 41.0 34.0 41.0 40-41 38.35410080388117 41.0 39.0 41.0 34.0 41.0 42-43 38.24115716821834 41.0 39.0 41.0 33.0 41.0 44-45 38.11531840217037 41.0 38.0 41.0 33.0 41.0 46-47 38.024307305719454 40.5 38.0 41.0 33.0 41.0 48-49 37.91212205725383 40.0 38.0 41.0 33.0 41.0 50-51 37.7752111624218 40.0 38.0 41.0 33.0 41.0 52-53 37.631192877738904 40.0 37.5 41.0 33.0 41.0 54-55 37.457334350075605 40.0 37.0 41.0 32.0 41.0 56-57 37.26700371734812 40.0 37.0 41.0 32.0 41.0 58-59 37.06536283059567 40.0 36.0 41.0 32.0 41.0 60-61 36.811894958045485 39.5 36.0 41.0 31.0 41.0 62-63 36.53078672132119 39.0 35.0 41.0 31.0 41.0 64-65 36.21415543370297 39.0 35.0 41.0 31.0 41.0 66-67 35.890268738696 38.0 35.0 40.5 31.0 41.0 68-69 35.51768056749785 37.0 35.0 40.0 30.0 41.0 70-71 35.13309139376464 37.0 35.0 39.5 30.0 41.0 72-73 34.732661795445786 36.0 35.0 39.0 30.0 41.0 74-75 34.32612826889436 36.0 34.5 39.0 29.0 40.5 76-77 33.92277352899754 35.0 34.0 37.5 29.0 39.5 78-79 33.520393759080264 35.0 34.0 37.0 29.0 39.0 80-81 33.1459119362825 35.0 34.0 37.0 29.0 39.0 82-83 33.00570087578201 35.0 34.0 36.0 29.0 38.0 84-85 32.67116548870341 35.0 34.0 36.0 29.0 37.0 86-87 32.1052309722181 35.0 34.0 36.0 28.0 37.0 88-89 16.692657681531117 18.0 17.0 18.5 2.0 19.0 90-91 2.7852575922109883 2.0 2.0 2.0 2.0 2.0 92-93 2.2998483225605595 2.0 2.0 2.0 2.0 2.0 94-95 2.1044283419070773 2.0 2.0 2.0 2.0 2.0 96-97 2.000019689343849 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08814163867314306 1101 2 0.10927176400370797 1101 3 0.11029942560259798 1101 4 0.07090664183655093 1101 5 -0.06455428606595603 1101 6 -0.020043879943045795 1101 7 0.002182903040214512 1101 8 0.009520854304682302 1101 9 0.13656483298933608 1101 10-11 0.0694958138974755 1101 12-13 0.07097170781685946 1101 14-15 0.09498968167096677 1101 16-17 0.15509170282573592 1101 18-19 0.1052748972287958 1101 20-21 0.07472456824138618 1101 22-23 0.15351213226814764 1101 24-25 0.10732370463231433 1101 26-27 0.11694031694401019 1101 28-29 0.14246468856079986 1101 30-31 0.06178263898575409 1101 32-33 0.15125089222516408 1101 34-35 0.15676997499977574 1101 36-37 0.15026076802524102 1101 38-39 0.15465035710990094 1101 40-41 0.15420352670605553 1101 42-43 0.16154108162837844 1101 44-45 0.10696055725322395 1101 46-47 0.10815840464677251 1101 48-49 0.11496117043478193 1101 50-51 -0.013969682368390579 1101 52-53 -0.01843761979756664 1101 54-55 -0.0650475381939799 1101 56-57 0.02557631614621414 1101 58-59 0.019025303488149348 1101 60-61 0.055992918864127716 1101 62-63 0.03287674524899842 1101 64-65 0.05307723740416037 1101 66-67 -9.449661945879484E-4 1101 68-69 0.05634679085655847 1101 70-71 0.01837461769999038 1101 72-73 0.004665507403331048 1101 74-75 -0.039132674452950766 1101 76-77 -0.09790071613623041 1101 78-79 -0.07222678668382798 1101 80-81 -0.07331494992431686 1101 82-83 -0.010990382896856943 1101 84-85 0.019614747869091786 1101 86-87 0.1413578247476579 1101 88-89 0.6312878394784036 1101 90-91 -0.13546366624470796 1101 92-93 -0.1877598654894479 1101 94-95 -0.008974069179891409 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.12810572066981507 1102 2 0.08676253174989768 1102 3 0.16510101000268662 1102 4 0.19080565799558968 1102 5 0.20788950122075533 1102 6 0.1247082009812388 1102 7 0.20904000102081 1102 8 0.1728383183618618 1102 9 0.27908230679555146 1102 10-11 0.26639591147817754 1102 12-13 0.27839084266147296 1102 14-15 0.26676207129690255 1102 16-17 0.20823233909524674 1102 18-19 0.2107638343212841 1102 20-21 0.20736391411668365 1102 22-23 0.22535592460991438 1102 24-25 0.19167843079703317 1102 26-27 0.23075137145651325 1102 28-29 0.19575494556544726 1102 30-31 0.2514260180764083 1102 32-33 0.24632358578655555 1102 34-35 0.14428849141201283 1102 36-37 0.15480750069654192 1102 38-39 0.19604102655324596 1102 40-41 0.11127219246822051 1102 42-43 0.10564904867299418 1102 44-45 0.1718459698860002 1102 46-47 0.19666764502478173 1102 48-49 0.13977214731600895 1102 50-51 0.17227112261372923 1102 52-53 0.1155648996079961 1102 54-55 0.15815974361926521 1102 56-57 0.11315815620594094 1102 58-59 0.10799502819502038 1102 60-61 0.04657978870807966 1102 62-63 0.016366672591544784 1102 64-65 0.0494649008924668 1102 66-67 0.06827967342931629 1102 68-69 0.06480555285443046 1102 70-71 0.07327391820612661 1102 72-73 0.03868041621818463 1102 74-75 0.16443784498149938 1102 76-77 0.06609015406518637 1102 78-79 -0.008606605802960132 1102 80-81 0.05212285433835717 1102 82-83 0.11352476016583779 1102 84-85 0.09092990284728586 1102 86-87 0.09404122636942702 1102 88-89 1.1374284956070753 1102 90-91 0.1006508299509079 1102 92-93 -0.05643596345289126 1102 94-95 0.07907829762606067 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.08169152571084837 1103 2 0.08197029436770009 1103 3 0.08965441124562545 1103 4 -0.03797436274964383 1103 5 0.024538880193595958 1103 6 -0.11564836878568485 1103 7 -0.03483084660811642 1103 8 -0.0391606709729615 1103 9 0.01187307250740588 1103 10-11 -0.013342468088659132 1103 12-13 0.06438670248044076 1103 14-15 0.034436078584327845 1103 16-17 0.10517219565171843 1103 18-19 0.07455320815215316 1103 20-21 0.11389711092156318 1103 22-23 0.11657865216471919 1103 24-25 0.13782948091360936 1103 26-27 0.11044786161139086 1103 28-29 0.10935947405467772 1103 30-31 0.046856969254186254 1103 32-33 0.0777284547103747 1103 34-35 0.13175624321392831 1103 36-37 0.10790111134221547 1103 38-39 0.12071699311263728 1103 40-41 0.10059039242034373 1103 42-43 0.13825253103511415 1103 44-45 0.08749022460909117 1103 46-47 0.034921543621372564 1103 48-49 0.08289164517499614 1103 50-51 0.04712794504695239 1103 52-53 0.019274218118233932 1103 54-55 0.003584932232442384 1103 56-57 0.002992105690410085 1103 58-59 0.09525111656616758 1103 60-61 0.06355120638762912 1103 62-63 0.09628834604030345 1103 64-65 0.1287566817022352 1103 66-67 0.10154651563257033 1103 68-69 0.1283792737867202 1103 70-71 0.1030692585077233 1103 72-73 0.09178732508987508 1103 74-75 0.12907187780151474 1103 76-77 0.07448421596399868 1103 78-79 0.15673533591535005 1103 80-81 0.13218837026520447 1103 82-83 0.1955985298582945 1103 84-85 0.1888026299588219 1103 86-87 0.11935861069702725 1103 88-89 0.6764836123211744 1103 90-91 -0.5298564087167872 1103 92-93 -0.3288589888879647 1103 94-95 -0.11804352298973608 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.020190916596227737 1104 2 0.12059165546068584 1104 3 0.16695062710329012 1104 4 0.1165126864324435 1104 5 0.14177561108833459 1104 6 0.16009974021302043 1104 7 0.2409621970748148 1104 8 0.19415946725468558 1104 9 0.193117121336428 1104 10-11 0.2194890054480183 1104 12-13 0.2484150969213772 1104 14-15 0.17649226833707843 1104 16-17 0.17352560694315855 1104 18-19 0.2808355049564426 1104 20-21 0.258945469200782 1104 22-23 0.05121125762750012 1104 24-25 0.09199396325534792 1104 26-27 0.1946712404856754 1104 28-29 0.17977319411159698 1104 30-31 0.15937288362152202 1104 32-33 0.23849512601316292 1104 34-35 0.21204222038282694 1104 36-37 0.19172863704592658 1104 38-39 0.17219502326166491 1104 40-41 0.20587824186776515 1104 42-43 0.18892842357703188 1104 44-45 0.10196293748715135 1104 46-47 0.14621191797009203 1104 48-49 0.2299845315865383 1104 50-51 0.2113334570348897 1104 52-53 0.17746543659709602 1104 54-55 0.14929661204072175 1104 56-57 0.2034746186091354 1104 58-59 0.18488752007963427 1104 60-61 0.19247683948523076 1104 62-63 0.15654629252523478 1104 64-65 -0.04220668583138831 1104 66-67 0.03921916645797552 1104 68-69 0.02547510235020667 1104 70-71 0.044634643822647035 1104 72-73 0.11358920592736865 1104 74-75 0.12339726910771986 1104 76-77 0.13620280715466038 1104 78-79 0.17381559782036504 1104 80-81 0.13061003948173067 1104 82-83 0.1576263144713721 1104 84-85 0.13228282125219692 1104 86-87 0.1878877487000068 1104 88-89 0.8294142631251837 1104 90-91 -0.6218815721399542 1104 92-93 -0.3288589888879647 1104 94-95 -0.11804352298973608 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.030854603614628218 1105 2 -0.0023446877670068034 1105 3 1.8796264747322766E-5 1105 4 0.08684331907634402 1105 5 0.06933909466092558 1105 6 0.07313314681136518 1105 7 0.08624140626226762 1105 8 -0.05817872895454457 1105 9 0.012033085241405672 1105 10-11 -0.02838314148255705 1105 12-13 0.03011151408848889 1105 14-15 -0.056250091205100716 1105 16-17 -0.015963622486694362 1105 18-19 -0.0024908288602176754 1105 20-21 0.06678896409521684 1105 22-23 -0.006165670943715895 1105 24-25 0.08343457618821049 1105 26-27 0.011644710784729284 1105 28-29 -0.03211796289318869 1105 30-31 0.01860320246228042 1105 32-33 -0.0124956887816694 1105 34-35 0.0743026657564485 1105 36-37 -0.0802926087863014 1105 38-39 -0.07582283623504082 1105 40-41 -0.018926972312343082 1105 42-43 -0.05230598461638891 1105 44-45 -0.08250508391808609 1105 46-47 -0.060243935516233194 1105 48-49 -0.03341300315304352 1105 50-51 -0.1640393310923045 1105 52-53 -0.12442192942858554 1105 54-55 -0.15286741371340895 1105 56-57 -0.0547938542393851 1105 58-59 -0.16858694803374163 1105 60-61 -0.12438458745565129 1105 62-63 -0.053721078793500965 1105 64-65 -0.11471871529271027 1105 66-67 -0.05615640103933828 1105 68-69 -0.005425720118552135 1105 70-71 -0.08766204576360082 1105 72-73 0.007436528586694635 1105 74-75 -0.06647918175924161 1105 76-77 0.016496459870317892 1105 78-79 -0.03211937922428376 1105 80-81 -0.19334256203935496 1105 82-83 -0.15298847811302352 1105 84-85 -0.15139931281358088 1105 86-87 -0.03350088388054928 1105 88-89 1.0645185763601965 1105 90-91 -0.7940948193128134 1105 92-93 -0.3288589888879647 1105 94-95 -0.11804352298973608 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.1287831974571887 1106 2 -0.1223203938161177 1106 3 -0.07407775393502192 1106 4 -0.12864343322107175 1106 5 -0.07238542061949715 1106 6 -0.14917317997681323 1106 7 -0.15773380246145052 1106 8 -0.08707215034604587 1106 9 -0.10142568460844359 1106 10-11 -0.25673932316815495 1106 12-13 -0.2407196607144826 1106 14-15 -0.12808987940763217 1106 16-17 -0.16722528965166816 1106 18-19 -0.22425246592911208 1106 20-21 -0.16215670606555221 1106 22-23 -0.19977178330964307 1106 24-25 -0.2111430475239544 1106 26-27 -0.30882743257917866 1106 28-29 -0.13035082958925415 1106 30-31 -0.08632535141612152 1106 32-33 -0.06671885960604129 1106 34-35 -0.2311881421111579 1106 36-37 -0.07071544391602202 1106 38-39 -0.1593348712135878 1106 40-41 -0.10475462932754454 1106 42-43 -0.4468093548840315 1106 44-45 -0.17512355146513414 1106 46-47 -0.3197343644692552 1106 48-49 -0.22523436860727486 1106 50-51 -0.22996005900301242 1106 52-53 -0.22333072449006153 1106 54-55 -0.1739049686880918 1106 56-57 -0.312155996922435 1106 58-59 -0.20560769262063872 1106 60-61 -0.15702250397800555 1106 62-63 -0.19283409302944676 1106 64-65 -0.14391872200265965 1106 66-67 -0.13837894231290448 1106 68-69 -0.16024415194500818 1106 70-71 -0.121260224605372 1106 72-73 -0.15842292173059036 1106 74-75 -0.2588288567709114 1106 76-77 -0.3240866738914647 1106 78-79 -0.28455543492078306 1106 80-81 -0.283240491922399 1106 82-83 -0.26570959796350024 1106 84-85 -0.28252063356774215 1106 86-87 -0.295272398384391 1106 88-89 0.8570109857339894 1106 90-91 -0.12560807984323485 1106 92-93 -0.30467802164927527 1106 94-95 -0.11804352298973608 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.12919049920045467 1107 2 -0.22544578586679265 1107 3 -0.13494077777457392 1107 4 -0.14605005213775968 1107 5 -0.20471117773817582 1107 6 -0.10571256125740547 1107 7 -0.19696338637777444 1107 8 -0.12985865738643554 1107 9 -0.09640121106964727 1107 10-11 -0.10868729427300394 1107 12-13 -0.23202559287810232 1107 14-15 -0.13862097100120963 1107 16-17 -0.15077089298593194 1107 18-19 -0.18683577220795655 1107 20-21 -0.22121411808579694 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0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23069.0 3 4014.0 4 2639.0 5 2181.0 6 2395.0 7 2686.0 8 3122.0 9 3568.0 10 4390.0 11 5398.0 12 6511.0 13 7349.0 14 8103.0 15 8608.0 16 9322.0 17 10701.0 18 13156.0 19 16349.0 20 19531.0 21 26613.0 22 31387.0 23 42683.0 24 64469.0 25 110836.0 26 241093.0 27 710808.0 28 750220.0 29 26422.0 30 905.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.448279568298396 19.106261303999762 14.456796483529516 23.988662644172326 2 14.660702662259295 19.91137384439693 40.87046358474963 24.557459908594144 3 18.743667820710687 24.694085640262696 29.15008597396217 27.412160565064447 4 12.532838812185169 16.911084454843273 38.13867665844113 32.41740007453043 5 13.8488507534565 37.67421552318308 34.70224435527568 13.774689368084742 6 31.734404186556763 36.765240015417916 17.990130311026775 13.510225486998548 7 28.307346487976993 32.10748250659709 21.60778085806624 17.97739014735968 8 25.759499066030184 35.50720676312746 20.329039048833277 18.404255122009072 9 25.720629984878585 17.488167862543364 20.432303866338543 36.35889828623951 10-11 24.62554012978403 26.768709326669477 27.905072436575434 20.70067810697106 12-13 25.384522905285717 23.85750385744826 28.01963320131954 22.738340035946482 14-15 22.367743202775227 25.59985323331456 25.6758309366383 26.35657262727192 16-17 22.645276422813193 28.557814765003663 26.298978514474946 22.4979302977082 18-19 22.603857697372018 27.87541246097779 27.423825024810206 22.09690481683999 20-21 23.30300102456382 27.115522755717624 27.20704551514156 22.374430704576994 22-23 23.009435424223465 27.00350171379437 27.02444204104258 22.962620820939588 24-25 22.809919537759065 27.098304955969994 27.193833019539245 22.897942486731697 26-27 22.584464968719423 27.318431820203397 27.06707997301865 23.03002323805853 28-29 22.62201346628668 27.617261326897385 26.94971488321875 22.81101032359718 30-31 22.872230520058114 27.20305226524743 26.890084353781834 23.03463286091262 32-33 22.611551502120573 27.253273513972793 26.868538477094145 23.266636506812493 34-35 23.104372357717175 27.376082711979677 26.704189177200284 22.81535575310287 36-37 23.104199506376453 26.963242302912516 26.920273195500172 23.012284995210855 38-39 22.701767130192426 27.368512245382036 26.834120289382398 23.09560033504314 40-41 23.197521644379872 27.139837889524713 26.774403667684645 22.88823679841077 42-43 22.834774438876863 27.06325792391852 26.809381207934297 23.29258642927032 44-45 22.894669118030286 27.19144286623305 26.88220413323626 23.0316838825004 46-47 23.070838089661102 27.36475968808373 26.527313984344886 23.03708823791028 48-49 22.76477416159704 27.39182594027816 26.672792767834125 23.170607130290684 50-51 22.82539833289129 27.430551614468662 26.85712829695244 22.886921755687617 52-53 23.124207793459245 27.247874477421654 26.738522734011326 22.88939499510778 54-55 22.830366301011164 27.2009749372017 26.85464614745995 23.114012614327184 56-57 22.854579602395706 27.315235197319655 26.82082419130074 23.009361008983902 58-59 22.977858058825273 27.093208890503156 26.855709075814627 23.07322397485694 60-61 22.670379999703503 27.324477606961782 26.96170723752483 23.043435155809885 62-63 22.757690138300298 27.298123373457138 27.0725297685575 22.871656719685063 64-65 22.62059850101412 27.372336492587994 26.831249658331657 23.175815348066227 66-67 22.6786263601862 27.34168840987933 26.72467070151511 23.25501452841937 68-69 23.00181999661342 27.09325670059654 26.973799878203614 22.931123424586424 70-71 22.9451737480357 26.954734893408844 26.76944658582145 23.33064477273401 72-73 22.931900813887985 27.062122891155454 26.850844649687193 23.15513164526937 74-75 22.717171146262636 27.50063932457675 26.748228422332254 23.033961106828357 76-77 22.95115004299226 27.042618858777836 26.781677142941856 23.224553955288048 78-79 23.01495215352567 27.29548067343412 26.6626160489243 23.026951124115914 80-81 23.056059499807276 27.230385707296822 26.583231720876448 23.13032307201945 82-83 23.08230071563221 27.30296486791126 26.733822528585677 22.88091188787085 84-85 23.050035070228482 27.38760153919851 26.737783098280953 22.824580292292055 86-87 22.982560785917055 27.308880367014552 26.697089063109868 23.011469783958518 88-89 32.20237485204901 17.279556251806774 32.47061721292627 18.047451683217943 90-91 30.981901948044605 13.674705078790177 15.559473735140836 39.78391923802438 92-93 23.264594288343375 16.50351052535863 33.20096650195617 27.03092868434183 94-95 14.984406099972944 6.417346037765133 26.126986990052202 52.471260872209726 96-97 25.65070398002468 6.807886156488589 14.380289726511068 53.16112013697566 98-99 57.45028060446048 14.125747946877528 18.742122510713386 9.681848937948603 100-101 31.130316335800117 6.7479450664844105 11.98844431936639 50.133294278349084 102-103 23.014504508638122 1.8744014836544909 36.49867999548076 38.61241401222663 104-105 42.40365070405908 4.446344315695887 38.399718155022114 14.750286825222911 106-107 4.965993711209105 1.4259232311984764 6.193130413326669 87.41495264426575 108-109 18.494363754204464 9.577060312679023 18.979794730530163 52.948781202586346 110-111 40.27104103289679 2.5082635853495363 22.80574504698618 34.41495033476749 112-113 27.506914118129405 2.676308201373117 37.36955895001021 32.447218730487265 114-115 26.964542700412736 3.6252511426771896 22.457873125006596 46.95233303190348 116-117 18.372348493490097 3.7916638149339534 19.335508254815625 58.50047943676032 118-119 19.04257247639898 3.991646440345134 14.724051181137884 62.241729902118 120-121 24.46932909576965 4.051342624286301 28.62542845623332 42.85389982371073 122-123 33.34869712786478 1.487668877967073 32.736285417778625 32.42734857638952 124-125 37.974864218584905 1.629701373570356 17.926807792833706 42.46862661501103 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 28.0 2 59.0 3 81.0 4 108.0 5 123.5 6 123.5 7 127.5 8 139.5 9 129.5 10 107.5 11 115.5 12 131.0 13 157.0 14 187.5 15 212.5 16 255.5 17 343.5 18 495.0 19 700.5 20 1079.5 21 1776.0 22 2797.5 23 4327.0 24 6505.5 25 9340.0 26 12660.0 27 16303.0 28 20409.5 29 24690.0 30 29214.0 31 34034.0 32 38864.5 33 43846.0 34 48769.5 35 53356.0 36 57796.0 37 62043.0 38 66281.0 39 70346.0 40 73792.0 41 76661.5 42 79089.5 43 81609.0 44 84189.0 45 85475.5 46 85351.5 47 84644.5 48 83641.5 49 82757.0 50 81648.0 51 79902.0 52 76485.5 53 71458.0 54 65719.5 55 59519.0 56 53396.0 57 47292.5 58 40946.5 59 34386.5 60 28407.5 61 22959.5 62 18046.5 63 14021.5 64 10527.5 65 7808.0 66 5739.0 67 4167.0 68 3029.5 69 2170.0 70 1545.5 71 1079.5 72 787.0 73 566.5 74 404.0 75 296.5 76 229.0 77 172.5 78 114.5 79 83.0 80 57.0 81 43.5 82 30.0 83 27.5 84 29.0 85 26.0 86 20.0 87 15.5 88 14.0 89 8.0 90 8.5 91 11.0 92 9.0 93 7.0 94 5.0 95 5.0 96 9.0 97 8.5 98 6.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.001065540961247665 3 0.04160242535653927 4 0.047254425237939934 5 0.04961714649983692 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.802294897263319E-4 12-13 0.0019226065170338306 14-15 0.0 16-17 2.316393394016663E-4 18-19 0.006578557239007324 20-21 0.002246901592196163 22-23 6.254262163844991E-4 24-25 0.0 26-27 0.0 28-29 0.0012276884988288313 30-31 0.0 32-33 0.0013666721024698314 34-35 6.949180182049989E-5 36-37 2.3163933940166633E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.949180182049989E-5 46-47 0.0 48-49 2.084754054614997E-4 50-51 2.3163933940166633E-5 52-53 0.0 54-55 0.001389836036409998 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.3163933940166633E-5 64-65 9.265573576066653E-5 66-67 0.0 68-69 3.474590091024995E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.4011474486316596E-4 80-81 0.0 82-83 7.875737539656655E-4 84-85 9.265573576066653E-5 86-87 0.0014129999703501644 88-89 19.31035872594657 90-91 26.271885284786666 92-93 40.389839742639424 94-95 44.778455503009454 96-97 48.40120211551576 98-99 93.01625922851127 100-101 51.60215665490556 102-103 53.04932342781748 104-105 74.88371705162037 106-107 81.693658826756 108-109 12.580100883565096 110-111 39.12995337563376 112-113 52.03298266225872 114-115 86.8335736205414 116-117 66.49362435882232 118-119 71.76036169093013 120-121 80.4086627553592 122-123 75.82660498265484 124-125 50.01501022919322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2158528.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.17983463883533 #Duplication Level Percentage of deduplicated Percentage of total 1 69.14674863118397 34.69772412125925 2 15.247983607403173 15.30282591990326 3 6.1326663152095815 9.232085447771173 4 3.1018905682714197 6.226094231344912 5 1.8120942646354692 4.546529527469488 6 1.0979317083294036 3.305641894122207 7 0.7582634754302517 2.663467506683103 8 0.5510908606489628 2.212291860667072 9 0.3904061717693154 1.7631465427228492 >10 1.6647152317173186 13.928816549430381 >50 0.06677856822511348 2.2803227963554344 >100 0.026019820225406404 2.337967022184896 >500 0.002488945342373974 0.8757442953833582 >1k 9.218316082865883E-4 0.6273422847025382 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6327867880333265E-5 2 0.0 0.0 0.0 0.0 4.6327867880333265E-5 3 0.0 0.0 0.0 0.0 4.6327867880333265E-5 4 0.0 0.0 0.0 0.0 4.6327867880333265E-5 5 0.0 0.0 0.0 0.0 4.6327867880333265E-5 6 9.265573576066653E-5 0.0 0.0 0.0 4.6327867880333265E-5 7 9.265573576066653E-5 0.0 0.0 0.0 4.6327867880333265E-5 8 9.265573576066653E-5 0.0 0.0 0.0 4.6327867880333265E-5 9 9.265573576066653E-5 0.0 0.0 0.0 4.6327867880333265E-5 10-11 9.265573576066653E-5 0.0 0.0 0.0 4.6327867880333265E-5 12-13 1.8531147152133306E-4 0.0 0.0 0.0 6.949180182049989E-5 14-15 4.632786788033326E-4 0.0 0.0 0.0 1.3898360364099978E-4 16-17 5.327704806238325E-4 0.0 0.0 0.0 1.8531147152133306E-4 18-19 6.25426216384499E-4 0.0 0.0 0.0 1.8531147152133306E-4 20-21 6.949180182049989E-4 0.0 0.0 0.0 1.8531147152133306E-4 22-23 6.949180182049989E-4 0.0 0.0 1.3898360364099978E-4 1.8531147152133306E-4 24-25 7.875737539656655E-4 0.0 0.0 1.8531147152133306E-4 1.8531147152133306E-4 26-27 8.107376879058321E-4 0.0 0.0 2.7796720728199957E-4 1.8531147152133306E-4 28-29 9.960491594271652E-4 0.0 0.0 0.0011813606309484983 1.8531147152133306E-4 30-31 0.001065540961247665 0.0 0.0 0.0034977540249651615 1.8531147152133306E-4 32-33 0.001065540961247665 0.0 0.0 0.008987606368784653 1.8531147152133306E-4 34-35 0.0010887048951878316 0.0 0.0 0.015890458682954307 1.8531147152133306E-4 36-37 0.0011581966970083317 0.0 0.0 0.02700914697423429 1.8531147152133306E-4 38-39 0.001204524564888665 0.0 0.0 0.04815781866160643 2.316393394016663E-4 40-41 0.001204524564888665 0.0 0.0 0.07354549026002906 2.316393394016663E-4 42-43 0.0012508524327689982 0.0 0.0 0.10111057164882735 2.316393394016663E-4 44-45 0.0013898360364099978 0.0 0.0 0.13483725946570996 2.316393394016663E-4 46-47 0.0016446393097518308 0.0 0.0 0.17280294719364309 2.316393394016663E-4 48-49 0.0017141311115723308 0.0 0.0 0.21248276603314853 2.7796720728199957E-4 50-51 0.0017141311115723308 0.0 0.0 0.25786091262193495 3.706229430426661E-4 52-53 0.0017372950455124974 0.0 0.0 0.3027526165979779 4.1695081092299935E-4 54-55 0.0018067868473329973 0.0 0.0 0.35436186141666914 4.632786788033326E-4 56-57 0.0018994425830936637 0.0 0.0 0.4125728274083079 5.096065466836659E-4 58-59 0.0019457704509739972 0.0 0.0 0.4785205473359623 5.096065466836659E-4 60-61 0.0019457704509739972 0.0 0.0 0.5519502179262905 5.096065466836659E-4 62-63 0.0019457704509739972 0.0 0.0 0.6246849704984138 5.096065466836659E-4 64-65 0.0019457704509739972 0.0 0.0 0.7028169196785958 5.559344145639991E-4 66-67 0.0022237376582559965 0.0 0.0 0.7916274424051947 6.022622824443325E-4 68-69 0.0022700655261363297 0.0 0.0 0.898992276217867 6.022622824443325E-4 70-71 0.0022700655261363297 0.0 0.0 1.0139085524950335 6.022622824443325E-4 72-73 0.00233955732795683 0.0 0.0 1.1316045008450204 6.254262163844991E-4 74-75 0.00240904912977733 0.0 0.0 1.2589134817801761 6.485901503246657E-4 76-77 0.00240904912977733 0.0 0.0 1.3971326756011504 6.485901503246657E-4 78-79 0.00240904912977733 0.0 0.0 1.4353068387345451 6.485901503246657E-4 80-81 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 82-83 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 84-85 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 86-87 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 88-89 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 90-91 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 92-93 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 94-95 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 96-97 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 98-99 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 100-101 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 102-103 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 104-105 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 106-107 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 108-109 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 110-111 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 112-113 0.00240904912977733 0.0 0.0 1.4361639042903311 6.485901503246657E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCGCC 615 0.0 1770.712 108-109 CCGCTCC 835 0.0 1748.783 108-109 CGTATCG 25 0.0053806147 1232.5364 110-111 CCGCGCG 265 0.0 933.9519 108-109 CGTCGCC 330 0.0 824.9909 108-109 CCGCGAC 90 1.1807242E-7 824.9908 108-109 CGACGCC 170 1.8189894E-12 727.93304 108-109 TGTCGCG 85 5.6916455E-5 725.02136 110-111 CCGCTCG 275 0.0 719.992 108-109 CGTCGCG 145 9.258656E-10 682.7511 108-109 CCTCGCC 1120 0.0 662.9391 108-109 CTCCGCC 560 0.0 660.2873 110-111 CGCGTGT 105 1.0723549E-4 586.92206 110-111 CCTCGCG 490 0.0 555.6061 108-109 CGTCGTC 175 6.9998714E-7 528.22986 110-111 CCGTTCG 115 2.718928E-4 430.43002 108-109 CGTCGCT 145 2.8213309E-4 425.01254 110-111 TTTTTCG 1100 0.0 419.90662 92-93 CGGCGCC 425 0.0 407.64252 108-109 GGTCGCC 400 0.0 371.2459 108-109 >>END_MODULE