FastQCFastQC Report
Fri 27 May 2016
SRR939911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158528
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46030.213247175853174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31270.1448672428618021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90850.042.5000841
ATCAACG104500.036.2031823
TCAACGC105350.035.9110834
CAACGCA106550.035.5066385
TATCAAC107850.035.1891372
GGTATCA58200.035.0140881
AACGCAG110400.034.3816576
ACGCAGA122800.030.8129987
CGCAGAG126150.029.9004148
GTACATG71450.028.4375271
TACATGG68200.027.3431932
GCAGAGT138400.026.4371269
AGAGTAC101250.026.05996110-11
ACATGGG69300.025.61963
GAGTACT73700.023.77349512-13
AGTACTT75450.023.65577512-13
GTACTTT73200.023.49022114-15
CATGGGG46000.021.8886094
TACTTTT84150.020.8577914-15
CAGAGTA131950.020.67307910-11
Produced by FastQC (version 0.11.5)