##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158528 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.857351862009665 34.0 31.0 34.0 31.0 34.0 2 32.93604762134195 34.0 31.0 34.0 31.0 34.0 3 32.957118925489965 34.0 33.0 34.0 31.0 34.0 4 36.38339970572538 37.0 37.0 37.0 35.0 37.0 5 36.26896477599549 37.0 37.0 37.0 35.0 37.0 6 36.390698661309926 37.0 37.0 37.0 35.0 37.0 7 36.38089151495834 37.0 37.0 37.0 35.0 37.0 8 36.406822612447 37.0 37.0 37.0 35.0 37.0 9 38.245974108281196 39.0 39.0 39.0 37.0 39.0 10-11 38.236722896344176 39.0 39.0 39.0 37.0 39.0 12-13 38.22654582196756 39.0 39.0 39.0 37.0 39.0 14-15 39.80097385811071 41.0 40.0 41.0 37.5 41.0 16-17 39.811694358377565 41.0 40.0 41.0 38.0 41.0 18-19 39.83390532807543 41.0 40.0 41.0 38.0 41.0 20-21 39.83643228162896 41.0 40.0 41.0 38.0 41.0 22-23 39.79091839438729 41.0 40.0 41.0 37.0 41.0 24-25 39.75274747420465 41.0 40.0 41.0 37.0 41.0 26-27 39.66517691686187 41.0 40.0 41.0 37.0 41.0 28-29 39.58461738740475 41.0 40.0 41.0 37.0 41.0 30-31 39.470484283734095 41.0 39.5 41.0 36.5 41.0 32-33 39.32989796750378 41.0 39.0 41.0 35.5 41.0 34-35 39.22721201670768 41.0 39.0 41.0 35.0 41.0 36-37 39.213064875693064 41.0 39.0 41.0 35.0 41.0 38-39 39.27250005559344 41.0 39.5 41.0 35.0 41.0 40-41 39.219097459009106 41.0 40.0 41.0 35.0 41.0 42-43 39.10061880133128 41.0 39.0 41.0 35.0 41.0 44-45 38.946916834064695 41.0 39.0 41.0 35.0 41.0 46-47 38.7758155094583 41.0 39.0 41.0 35.0 41.0 48-49 38.57055595294571 41.0 39.0 41.0 35.0 41.0 50-51 38.40643971261897 41.0 39.0 41.0 35.0 41.0 52-53 38.260463148960774 41.0 38.0 41.0 34.5 41.0 54-55 38.1065348700596 40.5 38.0 41.0 34.0 41.0 56-57 37.93842285112817 40.0 37.5 41.0 34.0 41.0 58-59 37.738155585658376 40.0 37.0 41.0 34.0 41.0 60-61 37.51630764113321 40.0 36.5 41.0 34.0 41.0 62-63 37.25760495115189 39.0 36.0 41.0 33.5 41.0 64-65 36.97287688647078 39.0 35.5 41.0 33.0 41.0 66-67 36.671282698209154 39.0 35.0 41.0 33.0 41.0 68-69 36.32981040783349 37.5 35.0 40.5 33.0 41.0 70-71 35.9699804681709 37.0 35.0 39.5 32.0 41.0 72-73 35.61461259710321 36.5 35.0 39.0 32.0 41.0 74-75 35.251235332597034 36.0 35.0 39.0 32.0 41.0 76-77 34.884025826859784 35.5 35.0 38.0 32.0 39.5 78-79 34.54041967488955 35.0 35.0 37.0 31.0 39.0 80-81 34.28864161132031 35.0 35.0 37.0 31.5 39.0 82-83 34.09027216695823 35.0 35.0 36.0 32.0 38.0 84-85 33.862784036157976 35.0 35.0 36.0 31.5 37.0 86-87 33.68650858362736 35.0 35.0 36.0 31.0 37.0 88-89 33.525266524223916 35.0 35.0 35.0 31.0 36.5 90-91 33.40536374788745 35.0 35.0 35.0 31.0 36.0 92-93 33.29265198320337 35.0 35.0 35.0 31.0 36.0 94-95 33.21340098437454 35.0 35.0 35.0 31.0 36.0 96-97 33.165387477021376 35.0 35.0 35.0 31.0 36.0 98-99 33.1009187742758 35.0 34.5 35.0 31.0 35.0 100-101 33.055956883579924 35.0 34.0 35.0 31.0 35.0 102-103 32.23767331255374 34.5 33.0 35.0 28.0 35.0 104-105 32.924594445844576 35.0 34.0 35.0 30.5 35.0 106-107 33.00223856257598 35.0 34.0 35.0 31.0 35.0 108-109 33.002631886174285 35.0 34.0 35.0 31.0 35.0 110-111 32.97164502846384 35.0 34.0 35.0 31.0 35.0 112-113 32.91640089913126 35.0 34.0 35.0 31.0 35.0 114-115 32.8807031921754 35.0 34.0 35.0 31.0 35.0 116-117 32.835523792139824 35.0 34.0 35.0 31.0 35.0 118-119 32.77260197690278 35.0 34.0 35.0 31.0 35.0 120-121 32.687631802784125 35.0 34.0 35.0 30.5 35.0 122-123 32.54440549300264 35.0 34.0 35.0 30.0 35.0 124-125 30.59726396877872 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0025300783825557005 1101 2 0.08088122930412567 1101 3 0.11182145435988389 1101 4 0.015961878259275863 1101 5 -0.02428031969404998 1101 6 0.04879696989478788 1101 7 0.0514637131333231 1101 8 0.07508812203225546 1101 9 0.0973675504071565 1101 10-11 0.07351604409925017 1101 12-13 0.06765574697320176 1101 14-15 0.1528746168563302 1101 16-17 0.15626299839002655 1101 18-19 0.23557310884722682 1101 20-21 0.21052249398029943 1101 22-23 0.22734046700480803 1101 24-25 0.22406037919571986 1101 26-27 0.2172144006065082 1101 28-29 0.19031870714974986 1101 30-31 0.23148022064516738 1101 32-33 0.2092607383429197 1101 34-35 0.22158272069201246 1101 36-37 0.13539036850828268 1101 38-39 0.08597477839182233 1101 40-41 0.06607777620654787 1101 42-43 0.09250018935165372 1101 44-45 0.08741191404185145 1101 46-47 0.09766600236903145 1101 48-49 0.10379224387361319 1101 50-51 0.10582390478360537 1101 52-53 0.03229852451201509 1101 54-55 -0.046310715606090014 1101 56-57 -0.05069139759904573 1101 58-59 -0.032182205700117095 1101 60-61 -0.025703266297988137 1101 62-63 -0.001663500935272566 1101 64-65 -0.06167442791084454 1101 66-67 -0.031756359818473356 1101 68-69 -0.043502585730301746 1101 70-71 -0.04610286818702747 1101 72-73 -0.1518512998602901 1101 74-75 -0.17576144792513304 1101 76-77 -0.16315916709483247 1101 78-79 -0.15699751856950428 1101 80-81 -0.15481464767648134 1101 82-83 -0.10640258790780877 1101 84-85 -0.16038897041272548 1101 86-87 -0.11799440039310127 1101 88-89 -0.12045888043332553 1101 90-91 -0.14636950530353943 1101 92-93 -0.14138011331814937 1101 94-95 -0.10359975989002379 1101 96-97 -0.1637494008076672 1101 98-99 -0.15307638054079575 1101 100-101 -0.13792643086203782 1101 102-103 -0.0853643911797306 1101 104-105 -0.11903693401229276 1101 106-107 -0.13559279004458347 1101 108-109 -0.0839803917769757 1101 110-111 -0.10939081788084337 1101 112-113 -0.12341537155764115 1101 114-115 -0.08145905531410591 1101 116-117 -0.07630257276041164 1101 118-119 -0.04943962819237413 1101 120-121 -0.034942111230144235 1101 122-123 -0.10896937370272042 1101 124-125 -0.02524928501923185 1102 1 0.08553718279522116 1102 2 0.10245617823448327 1102 3 0.12031891915726334 1102 4 0.053852708832636154 1102 5 -0.05018388901741844 1102 6 -0.006641461736883514 1102 7 -0.03605854943910458 1102 8 -0.024527467771328304 1102 9 0.01144756116720913 1102 10-11 0.0015708120584747576 1102 12-13 6.873790081982634E-4 1102 14-15 0.0335520805885281 1102 16-17 0.11480981771948962 1102 18-19 0.01582053144387885 1102 20-21 0.07254159759187218 1102 22-23 0.11168499278154798 1102 24-25 0.10401490171375372 1102 26-27 0.09850851389941795 1102 28-29 0.0755902947363225 1102 30-31 0.08748233654864634 1102 32-33 0.19162049880530674 1102 34-35 0.20660586564314087 1102 36-37 0.286663476223481 1102 38-39 0.191817908159706 1102 40-41 0.12223147619418029 1102 42-43 0.1031170555577603 1102 44-45 0.1167434456075398 1102 46-47 0.2284022537354744 1102 48-49 0.20473230440795476 1102 50-51 0.21089433259509605 1102 52-53 0.26800105592333523 1102 54-55 0.21189653461303948 1102 56-57 0.14870576398502777 1102 58-59 0.16097940455299664 1102 60-61 0.07336066756738546 1102 62-63 0.061985791672334756 1102 64-65 0.0857900083913421 1102 66-67 0.06936119198009294 1102 68-69 0.09736014593359954 1102 70-71 0.05477637366236365 1102 72-73 0.0999794666762952 1102 74-75 0.10100497943862052 1102 76-77 0.07977796692112094 1102 78-79 0.11486234164004827 1102 80-81 0.1434358955167454 1102 82-83 0.13910517928142951 1102 84-85 0.09553605583295877 1102 86-87 0.04369946429920901 1102 88-89 0.06739931792540688 1102 90-91 0.14648668005826693 1102 92-93 0.1428859687669899 1102 94-95 0.11368892733810299 1102 96-97 0.1101061923900275 1102 98-99 0.08204335215488356 1102 100-101 0.13292918761096217 1102 102-103 0.07959483886249075 1102 104-105 0.10134865419352934 1102 106-107 0.1285611509610689 1102 108-109 0.1362913447533174 1102 110-111 0.04060893387962494 1102 112-113 0.10276266041741167 1102 114-115 0.12126214641797617 1102 116-117 0.028626584191059123 1102 118-119 0.05773462963913545 1102 120-121 0.03649738918554846 1102 122-123 0.08685990881281924 1102 124-125 0.08465831571411542 1103 1 0.0447280300695283 1103 2 0.07548028348550417 1103 3 0.07375828367936776 1103 4 0.05650614819715827 1103 5 -0.13297376818087514 1103 6 0.05088755055954408 1103 7 -0.02017825120422856 1103 8 0.04821914783015302 1103 9 0.11459569453955254 1103 10-11 0.11304784602214824 1103 12-13 0.10285321675210923 1103 14-15 0.05812853208990276 1103 16-17 0.055291390810971563 1103 18-19 0.05958698799857842 1103 20-21 0.08729110334113699 1103 22-23 0.06925434303022371 1103 24-25 0.13722145862572432 1103 26-27 0.12521511957154985 1103 28-29 0.16433046513092364 1103 30-31 0.03903069333119191 1103 32-33 0.0905628465627899 1103 34-35 0.09768829691347491 1103 36-37 0.09760015893667173 1103 38-39 0.042025360485567376 1103 40-41 0.09886693635712618 1103 42-43 0.10070387080994436 1103 44-45 0.029520602801433427 1103 46-47 0.047087369474368757 1103 48-49 0.09211073645845147 1103 50-51 0.11457350845969927 1103 52-53 0.16368134883892793 1103 54-55 0.10738067869488788 1103 56-57 0.0684857669992951 1103 58-59 0.04063555675562469 1103 60-61 0.06942261956965723 1103 62-63 0.01687865229806107 1103 64-65 0.009934267547166087 1103 66-67 0.016099137138212427 1103 68-69 0.04046013972776308 1103 70-71 0.11723348688346391 1103 72-73 0.08570736724103512 1103 74-75 0.07748684509108017 1103 76-77 0.0837763002101255 1103 78-79 0.0759364393554165 1103 80-81 -0.048157187632625664 1103 82-83 -0.03058727707822584 1103 84-85 -0.08919669415152498 1103 86-87 -0.0445803658381152 1103 88-89 -0.03759771213744756 1103 90-91 -0.007568613965510451 1103 92-93 -0.06707395924527759 1103 94-95 -0.05673094198466799 1103 96-97 -0.08949973684568135 1103 98-99 -0.07350023499725467 1103 100-101 -0.03553185301955608 1103 102-103 -0.11800283660760869 1103 104-105 -0.04763935080660531 1103 106-107 -0.05674320110503572 1103 108-109 -0.05388880188178291 1103 110-111 0.011270830915890429 1103 112-113 0.006841669610921031 1103 114-115 0.03084484808557164 1103 116-117 0.04890105957604618 1103 118-119 0.052829800609998756 1103 120-121 0.08346406383115124 1103 122-123 0.03111862136723431 1103 124-125 0.035223490319822304 1104 1 0.07483373356480172 1104 2 0.10345586897374659 1104 3 0.1074796244447569 1104 4 0.06588251897250075 1104 5 0.02106286428550419 1104 6 0.04634647276904502 1104 7 0.061544031049784564 1104 8 0.06880190788963603 1104 9 0.05461484527402405 1104 10-11 0.12331140934260532 1104 12-13 0.12186941463589562 1104 14-15 0.05696317245195104 1104 16-17 0.09947475641881454 1104 18-19 0.0825833825251081 1104 20-21 0.17432022529600744 1104 22-23 0.09894226094953495 1104 24-25 0.14273023693724696 1104 26-27 0.16326490078243694 1104 28-29 0.1518725913973853 1104 30-31 0.15993120580056086 1104 32-33 0.1879071532313077 1104 34-35 0.13043337817036615 1104 36-37 0.14818992666715047 1104 38-39 0.19411027634001954 1104 40-41 0.18906288367656288 1104 42-43 0.2315924541905332 1104 44-45 0.1925947236329435 1104 46-47 0.22046009198129468 1104 48-49 0.1355377015484862 1104 50-51 0.1374380410854883 1104 52-53 0.10876367559967548 1104 54-55 0.14348222038250924 1104 56-57 0.14980845152571476 1104 58-59 0.12750393022704287 1104 60-61 0.08983339747462793 1104 62-63 0.12352738075348668 1104 64-65 0.09085842421943369 1104 66-67 0.07433796498487766 1104 68-69 0.08310663214770386 1104 70-71 0.18198650660122695 1104 72-73 0.11659006631772684 1104 74-75 -0.01947310954628989 1104 76-77 0.0570802833489239 1104 78-79 0.15759238945651788 1104 80-81 0.13583402870647632 1104 82-83 0.11767524846500521 1104 84-85 0.11697647556249535 1104 86-87 0.08222183596141264 1104 88-89 0.11370646357856629 1104 90-91 0.09731535826058035 1104 92-93 0.11754821495151191 1104 94-95 0.14066311379853857 1104 96-97 0.09029944791075906 1104 98-99 0.12225584455852356 1104 100-101 0.16101379313050046 1104 102-103 0.16243800474086356 1104 104-105 0.1892324997534729 1104 106-107 0.10897564685525651 1104 108-109 0.14263410945780208 1104 110-111 0.14517966700058338 1104 112-113 0.12453256177837346 1104 114-115 0.1839292931957175 1104 116-117 0.1274300370980086 1104 118-119 0.19941529954547121 1104 120-121 0.17436906976895017 1104 122-123 0.24393543763786596 1104 124-125 0.2283137127465089 1105 1 -0.01201177330749914 1105 2 0.009167781129299613 1105 3 -0.05463643673817131 1105 4 0.012406722483703447 1105 5 -0.04676858326563149 1105 6 0.05766065501602213 1105 7 -0.024369160637853327 1105 8 0.02293943970678214 1105 9 0.04649540593019452 1105 10-11 -0.00858937327488718 1105 12-13 0.06502664626063392 1105 14-15 -0.012498345022827095 1105 16-17 -0.03248365725568192 1105 18-19 -0.06817103253499823 1105 20-21 0.02093828364031225 1105 22-23 0.0038570967572226778 1105 24-25 -0.03425417713167889 1105 26-27 -0.019486553022289854 1105 28-29 -0.005823913646857193 1105 30-31 -0.10436982859516064 1105 32-33 -0.010780820577224404 1105 34-35 0.10414891576900942 1105 36-37 0.08360773650305475 1105 38-39 0.06702556992632225 1105 40-41 0.04194607145060303 1105 42-43 -0.017416628831909975 1105 44-45 -0.11154224617921926 1105 46-47 -0.02992263341591439 1105 48-49 -0.047257946284887 1105 50-51 -0.12657667731424738 1105 52-53 -0.11344185677743468 1105 54-55 -0.1592490216791731 1105 56-57 -0.09132234338436263 1105 58-59 -0.17434322689626924 1105 60-61 -0.117552574267755 1105 62-63 -0.12160943680640912 1105 64-65 -0.056926969577460795 1105 66-67 -0.07742233682202482 1105 68-69 -0.0734753148215006 1105 70-71 0.02039311005766109 1105 72-73 -0.028278981410799986 1105 74-75 -0.04276586611944566 1105 76-77 -0.03868894309719906 1105 78-79 -0.03565338349960001 1105 80-81 -0.08194888205019168 1105 82-83 -0.03675258447441365 1105 84-85 -0.15902361231464823 1105 86-87 -0.20354600079964769 1105 88-89 -0.09365222537619644 1105 90-91 -0.07645346762925698 1105 92-93 -0.15628922663828604 1105 94-95 -0.07414939622815098 1105 96-97 -0.06726299339803887 1105 98-99 -0.15156121157759372 1105 100-101 -0.06906823916128246 1105 102-103 -0.09644708976283312 1105 104-105 -0.11704770162823763 1105 106-107 -0.11806609172250404 1105 108-109 -0.12400537660396083 1105 110-111 -0.11347260416375349 1105 112-113 -0.15218438900735975 1105 114-115 -0.018448498819523707 1105 116-117 -0.14750964954845358 1105 118-119 -0.1169114562858411 1105 120-121 -0.3337978334507241 1105 122-123 -0.2958184961415995 1105 124-125 -0.26564422271284016 1106 1 -0.03336755733013064 1106 2 0.06159831098806734 1106 3 0.012761910049590597 1106 4 0.020827211105903132 1106 5 -0.007014226487946473 1106 6 0.06569729526721346 1106 7 0.010158247023916545 1106 8 0.004974941240696751 1106 9 0.02592784318973429 1106 10-11 0.06267513574950101 1106 12-13 0.05432778566864016 1106 14-15 -0.012967695865178541 1106 16-17 0.04844080552928176 1106 18-19 0.02602343353586889 1106 20-21 0.05296224012038664 1106 22-23 0.04722026961430714 1106 24-25 0.060036596396798814 1106 26-27 0.05327067225723425 1106 28-29 -0.01671193550549077 1106 30-31 -0.04177175730988125 1106 32-33 0.01436697091318706 1106 34-35 0.08558851490894881 1106 36-37 0.042764740354364505 1106 38-39 -0.029633419772927994 1106 40-41 0.0208594253282115 1106 42-43 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124-125 0.031555224712665364 2216 1 -0.12364262219618638 2216 2 -0.25122084543910717 2216 3 -0.12163112658710418 2216 4 -0.04289386736964218 2216 5 0.014092220170518033 2216 6 -0.04700521733441576 2216 7 -0.028017131454092237 2216 8 -0.03022090920397602 2216 9 -0.10766078017515213 2216 10-11 -0.04460940769526189 2216 12-13 -0.07437217403267482 2216 14-15 -0.03785394862961766 2216 16-17 -0.13332136668303463 2216 18-19 -0.20706237301717323 2216 20-21 -0.22731863353016735 2216 22-23 -0.12566425416874694 2216 24-25 -0.1423363447323993 2216 26-27 -0.1154149468854655 2216 28-29 -0.29423057413551135 2216 30-31 -0.2592100092285534 2216 32-33 -0.37982896496625784 2216 34-35 -0.28687531087588525 2216 36-37 -0.20718187266394494 2216 38-39 -0.143567519522513 2216 40-41 -0.07963211721421004 2216 42-43 -0.13237919300236456 2216 44-45 -0.11719807190035425 2216 46-47 -0.03237177511310563 2216 48-49 -0.07529804454474487 2216 50-51 -0.15193595831096474 2216 52-53 -0.21440092007266287 2216 54-55 -0.25981828164122334 2216 56-57 -0.30201472236145577 2216 58-59 -0.3023531705586535 2216 60-61 -0.20520684077556695 2216 62-63 -0.22010832623465149 2216 64-65 -0.20726970231946495 2216 66-67 -0.19899317070696299 2216 68-69 -0.23604536909977725 2216 70-71 -0.2472693938035917 2216 72-73 -0.19170954493403514 2216 74-75 -0.21487506989097938 2216 76-77 -0.23670903635580487 2216 78-79 -0.24381204081098673 2216 80-81 -0.1957530683908928 2216 82-83 -0.1307466712313996 2216 84-85 -0.17831152252749405 2216 86-87 -0.23621225559863746 2216 88-89 -0.18697702716487896 2216 90-91 -0.1651631450486093 2216 92-93 -0.1511629450678882 2216 94-95 -0.12552988841001422 2216 96-97 -0.19257741023753994 2216 98-99 -0.16401653128987448 2216 100-101 -0.10183746087847823 2216 102-103 -0.06938517722586823 2216 104-105 -0.17344783107236594 2216 106-107 -0.12068439335673986 2216 108-109 -0.1806224720542957 2216 110-111 -0.13770660384014377 2216 112-113 -0.07843801123644312 2216 114-115 -0.11929724041668521 2216 116-117 -0.26094241950564623 2216 118-119 -0.234514386430142 2216 120-121 -0.35468173089567756 2216 122-123 -0.3770483624317009 2216 124-125 -0.38143787700876786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 211.0 3 4.0 4 33.0 5 61.0 6 154.0 7 374.0 8 677.0 9 1291.0 10 2499.0 11 3766.0 12 4779.0 13 7061.0 14 11377.0 15 12003.0 16 5068.0 17 3950.0 18 3828.0 19 3381.0 20 3167.0 21 3134.0 22 3215.0 23 3563.0 24 4335.0 25 5912.0 26 7568.0 27 9766.0 28 12885.0 29 16368.0 30 21269.0 31 27391.0 32 35569.0 33 47786.0 34 68118.0 35 115183.0 36 291098.0 37 874798.0 38 543666.0 39 3220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.578977120034104 19.07988990927709 14.708509790475485 23.632623180213326 2 14.919380244314645 20.240784460521244 40.24816912266137 24.591666172502745 3 18.697556853559462 24.992077934592462 29.256372861505618 27.053992350342455 4 12.55123862187565 17.161927016930058 37.90263549974798 32.384198861446315 5 13.58691920719544 37.58603221023684 34.54964492287023 14.277403659697493 6 31.611589008806 36.966488273490086 17.6185808106265 13.803341907077415 7 27.895769709728114 32.52619377649954 21.5357873513802 18.04224916239215 8 25.824960343345094 34.992921101787886 20.1487773149109 19.033341239956115 9 25.88917076822724 16.890770006226465 20.53802406084146 36.682035164704835 10-11 24.550480697957124 26.594026114092568 27.809947334479794 21.04554585347051 12-13 25.674470211753153 23.805070817953442 28.117895185491037 22.40256378480237 14-15 22.53146588786432 25.16939784890444 26.38965072493848 25.909485538292763 16-17 22.140520396668744 28.268195919762857 26.980650920894416 22.610632762673983 18-19 22.03645261956296 27.027423318113087 29.102633831944736 21.833490230379223 20-21 22.80169122770604 26.272127173800243 29.01657314198455 21.90960845650917 22-23 22.46508141454264 26.111443900707936 28.903262928644196 22.520211756105223 24-25 22.256278352655144 26.28427798944466 29.04389009547247 22.41555356242773 26-27 22.067515454976725 26.51918344353189 28.942918507427283 22.4703825940641 28-29 22.131123617576424 26.767037536691674 28.791449543392535 22.310389302339374 30-31 22.397277218548936 26.407510118006343 28.693836725768673 22.501375937676045 32-33 22.184956600053372 26.435005707593323 28.67389257864619 22.70614511370712 34-35 22.571053050968068 26.536672213656715 28.594648760636876 22.29762597473834 36-37 22.63350301687076 26.2033895321256 28.662403267411868 22.50070418359178 38-39 22.33793121979423 26.62223978563169 28.43222325584843 22.60760573872565 40-41 22.792129636493016 26.445105182791234 28.334332471017287 22.428432709698463 42-43 22.544785149879917 26.39738747887449 28.27674693124203 22.78108044000356 44-45 22.65791780324369 26.537204984137336 28.14874303228867 22.656134180330298 46-47 22.83303714383135 26.77801724137931 27.672909501289766 22.71603611349957 48-49 22.70195244166395 26.851168944762353 27.62046635484923 22.826412258724464 50-51 22.77035553858926 27.07247716962671 27.57617691315563 22.5809903786284 52-53 23.08276751564029 26.905326222314468 27.406060982299046 22.605845279746198 54-55 22.799727406825394 26.875166780324367 27.44430463723426 22.880801175615975 56-57 22.78399909568002 27.005857695614786 27.42165030984078 22.788492898864412 58-59 22.83252753728467 26.802038240875266 27.50221447208468 22.86321974975539 60-61 22.588518657158954 26.9934649909568 27.667025862068968 22.75099048981528 62-63 22.676379458594003 26.930389598837728 27.74828494233107 22.644946000237198 64-65 22.468251512141606 26.965899909568 27.62899068253921 22.936857895751178 66-67 22.549000985857028 26.896732402822664 27.500523504907047 23.05374310641326 68-69 22.794353374151274 26.73382045542147 27.686066615767785 22.78575955465947 70-71 22.723772867435585 26.64758112936223 27.510437668633443 23.11820833456874 72-73 22.798546046194442 26.71169889850861 27.437424948854034 23.05233010644291 74-75 22.62569677113292 27.172452708512466 27.253688624840635 22.94816189551398 76-77 22.723819195303467 26.763771422006105 27.344421754084262 23.167987628606163 78-79 22.759700128976785 27.019061137960687 27.1682600364693 23.052978696593236 80-81 22.88700910991194 26.944380614937586 27.05406184214428 23.114548433006195 82-83 22.941699157944672 26.942874959231478 27.213499199454443 22.901926683369407 84-85 22.88022207726747 26.985635581285024 27.239628116938952 22.894514224508555 86-87 22.81191163607792 26.908870304207312 27.121909004655027 23.157309055059745 88-89 22.80609748865894 27.003657121890473 26.97206151599609 23.2181838734545 90-91 22.746192567692 26.973834015478744 27.12310375428218 23.156869662547077 92-93 22.718514654431168 26.931177172591692 27.233211707237526 23.117096465739614 94-95 22.566309568814074 26.886739568739948 27.273232671395853 23.27371819105013 96-97 22.705774490764078 26.623907588875383 27.40392990037655 23.26638801998399 98-99 22.61968822236435 26.74504231770909 27.124450455838762 23.510819004087804 100-101 22.45097641795159 26.57943899255395 27.43094540143686 23.5386391880576 102-103 22.45003520931008 26.45402868632211 27.352862146285418 23.743073958082388 104-105 22.417643205338585 26.623253279863455 27.179711221909553 23.779392292888403 106-107 22.445613019053688 26.568784386401806 27.14278102739329 23.842821567151212 108-109 22.36079704575494 26.457027751872698 27.22241293923015 23.959762263142217 110-111 22.296705338275814 26.643334903918635 27.23709954128717 23.82286021651839 112-113 22.380484098330083 26.642157118251617 27.22992296171715 23.747435821701142 114-115 22.150199474338443 26.807164304679855 27.124502472406935 23.918133748574768 116-117 22.113859782987884 26.727903721895373 27.172356505475804 23.98587998964094 118-119 22.01920292792532 26.801380572381607 27.143327040455866 24.03608945923721 120-121 22.01690097518455 26.859652784357017 27.115106245522092 24.008339994936332 122-123 21.885183548250502 26.851187812310922 27.115166694000386 24.14846194543819 124-125 21.8537279841223 26.744788855589004 27.129546967591832 24.27193619269687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 63.0 1 57.5 2 100.0 3 138.0 4 93.5 5 74.5 6 185.0 7 470.5 8 840.5 9 977.0 10 760.5 11 581.0 12 643.5 13 796.5 14 927.0 15 1066.0 16 1246.0 17 1438.5 18 1632.0 19 1873.5 20 2201.0 21 2616.5 22 3261.5 23 4152.5 24 5329.5 25 7107.5 26 9724.5 27 13427.0 28 17132.5 29 20860.0 30 24486.5 31 29459.0 32 35672.0 33 39775.5 34 43093.5 35 46649.0 36 51025.5 37 55196.5 38 58427.5 39 61821.0 40 65632.0 41 69477.0 42 72074.0 43 74488.0 44 77605.5 45 79422.0 46 79397.5 47 81970.5 48 85239.0 49 83056.5 50 79465.0 51 77722.5 52 76138.5 53 75578.5 54 74597.5 55 70613.0 56 64805.5 57 58437.0 58 53592.5 59 47651.0 60 38728.5 61 31479.0 62 26071.5 63 19464.5 64 13650.5 65 10234.0 66 7437.5 67 5423.5 68 3968.0 69 2858.0 70 2011.5 71 1388.0 72 988.5 73 811.5 74 616.0 75 392.5 76 272.0 77 165.5 78 103.5 79 58.5 80 26.5 81 10.5 82 7.0 83 5.5 84 4.5 85 4.5 86 3.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.0 98 6.5 99 11.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014268983307142645 2 0.0 3 0.0 4 0.0 5 0.1362039315681798 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.3163933940166633E-5 14-15 0.0 16-17 6.949180182049989E-5 18-19 0.0 20-21 0.004447475316511993 22-23 3.2429507516233284E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.033934236664986E-4 92-93 0.0 94-95 7.412458860853322E-4 96-97 0.0 98-99 0.0015983114418714978 100-101 2.3163933940166633E-5 102-103 3.706229430426661E-4 104-105 0.0025017048655379963 106-107 0.0070881637856909895 108-109 3.2429507516233284E-4 110-111 0.004493803184392326 112-113 0.00859381949180182 114-115 0.004864426127434993 116-117 0.001436163904290331 118-119 1.3898360364099978E-4 120-121 6.254262163844991E-4 122-123 0.0010192130933673318 124-125 0.0013435081685296646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2158528.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.24625472496925 #Duplication Level Percentage of deduplicated Percentage of total 1 67.32576071985899 32.48215801242646 2 15.973748544573201 15.413470823881708 3 6.610407895483917 9.567822694843949 4 3.256649824787531 6.284846279869027 5 1.952770180899539 4.7106923783501715 6 1.2077874061349736 3.4962731309998696 7 0.8013114647143864 2.7062193928453953 8 0.56972564182212 2.1989702750957276 9 0.42784073361780267 1.8577541714258003 >10 1.768284333303698 14.347360907501521 >50 0.07056229924650385 2.2962901720571356 >100 0.03102436841397933 2.676757768362432 >500 0.002974981428951586 0.9713392846257839 >1k 0.0011516057144327816 0.9900447077149485 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4603 0.213247175853174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3127 0.1448672428618021 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.3898360364099978E-4 0.0 0.0 0.0 0.0 16-17 1.6214753758116642E-4 0.0 0.0 0.0 0.0 18-19 1.8531147152133306E-4 0.0 0.0 0.0 0.0 20-21 1.8531147152133306E-4 0.0 0.0 0.0 4.6327867880333265E-5 22-23 1.8531147152133306E-4 0.0 0.0 9.265573576066653E-5 4.6327867880333265E-5 24-25 3.706229430426661E-4 0.0 0.0 1.3898360364099978E-4 4.6327867880333265E-5 26-27 3.706229430426661E-4 0.0 0.0 2.316393394016663E-4 6.949180182049989E-5 28-29 5.327704806238325E-4 0.0 0.0 0.0011350327630681649 1.3898360364099978E-4 30-31 6.485901503246657E-4 0.0 0.0 0.0034282622231446612 1.3898360364099978E-4 32-33 6.717540842648323E-4 0.0 0.0 0.00880229489726332 1.3898360364099978E-4 34-35 7.412458860853322E-4 0.0 0.0 0.015705147211432978 1.3898360364099978E-4 36-37 7.875737539656655E-4 0.0 0.0 0.02666168796513179 1.3898360364099978E-4 38-39 9.265573576066653E-4 0.0 0.0 0.0476945399828031 1.3898360364099978E-4 40-41 9.265573576066653E-4 0.0 0.0 0.07312853944910605 1.3898360364099978E-4 42-43 9.960491594271652E-4 0.0 0.0 0.10057780116820352 1.3898360364099978E-4 44-45 0.001204524564888665 0.0 0.0 0.13430448898508612 1.6214753758116642E-4 46-47 0.0013666721024698312 0.0 0.0 0.17227017671301925 1.8531147152133306E-4 48-49 0.0014361639042903313 0.0 0.0 0.21188050375070416 1.8531147152133306E-4 50-51 0.0014824917721706645 0.0 0.0 0.25725865033949064 1.8531147152133306E-4 52-53 0.0014824917721706645 0.0 0.0 0.3020576985797729 2.316393394016663E-4 54-55 0.0014824917721706645 0.0 0.0 0.353504795860883 2.316393394016663E-4 56-57 0.0016214753758116642 0.0 0.0 0.41115982743795776 2.5480327334183295E-4 58-59 0.0016214753758116642 0.0 0.0 0.47666743262074895 2.7796720728199957E-4 60-61 0.0016214753758116642 0.0 0.0 0.5500971032110772 2.7796720728199957E-4 62-63 0.0016214753758116642 0.0 0.0 0.6225538885759183 2.7796720728199957E-4 64-65 0.0016214753758116642 0.0 0.0 0.7002457230112373 2.7796720728199957E-4 66-67 0.0016678032436919976 0.0 0.0 0.7889635900020755 2.7796720728199957E-4 68-69 0.001760458979452664 0.0 0.0 0.8960967844753462 2.7796720728199957E-4 70-71 0.0018531147152133307 0.0 0.0 1.010758257479171 3.2429507516233284E-4 72-73 0.001968934384914164 0.0 0.0 1.1285931894327987 3.706229430426661E-4 74-75 0.0019920983188543304 0.0 0.0 1.2556473670946127 3.706229430426661E-4 76-77 0.002015262252794497 0.0 0.0 1.3943530035283302 3.706229430426661E-4 78-79 0.0020384261867346636 0.0 0.0 1.5510338526996175 3.9378687698283274E-4 80-81 0.0022700655261363297 0.0 0.0 1.7217983737065259 4.169508109229994E-4 82-83 0.0022700655261363297 0.0 0.0 1.9009714027337148 4.632786788033326E-4 84-85 0.0022700655261363297 0.0 0.0 2.0855184644350224 4.632786788033326E-4 86-87 0.002316393394016663 0.0 0.0 2.284126034038011 4.864426127434993E-4 88-89 0.0023858851958371632 0.0 0.0 2.5039980949980727 5.096065466836659E-4 90-91 0.002571196667358496 0.0 0.0 2.7437448112787974 5.096065466836659E-4 92-93 0.002640688469178996 0.0 0.0 2.9956062650102293 6.022622824443325E-4 94-95 0.002640688469178996 0.0 0.0 3.251822538322412 6.022622824443325E-4 96-97 0.002640688469178996 0.0 0.0 3.5247631719393957 6.485901503246657E-4 98-99 0.002756508138879829 0.0 0.0 3.8193389198564947 6.485901503246657E-4 100-101 0.0028259999407003293 0.0 0.0 4.145046994989178 6.485901503246657E-4 102-103 0.002895491742520829 0.0 0.0 4.489031414000653 6.485901503246657E-4 104-105 0.0037293933643668276 0.0 0.0 4.839246931242031 6.485901503246657E-4 106-107 0.003845213034067661 0.0 0.0 5.20324498917781 6.485901503246657E-4 108-109 0.0038915409019479943 0.0 0.0 5.585241423785098 6.485901503246657E-4 110-111 0.004053688439529161 0.0 0.0 5.993250956207193 6.949180182049989E-4 112-113 0.004076852373469327 0.0 0.0 6.423405209476087 6.949180182049989E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9085 0.0 42.500084 1 ATCAACG 10450 0.0 36.203182 3 TCAACGC 10535 0.0 35.911083 4 CAACGCA 10655 0.0 35.506638 5 TATCAAC 10785 0.0 35.189137 2 GGTATCA 5820 0.0 35.014088 1 AACGCAG 11040 0.0 34.381657 6 ACGCAGA 12280 0.0 30.812998 7 CGCAGAG 12615 0.0 29.900414 8 GTACATG 7145 0.0 28.437527 1 TACATGG 6820 0.0 27.343193 2 GCAGAGT 13840 0.0 26.437126 9 AGAGTAC 10125 0.0 26.059961 10-11 ACATGGG 6930 0.0 25.6196 3 GAGTACT 7370 0.0 23.773495 12-13 AGTACTT 7545 0.0 23.655775 12-13 GTACTTT 7320 0.0 23.490221 14-15 CATGGGG 4600 0.0 21.888609 4 TACTTTT 8415 0.0 20.85779 14-15 CAGAGTA 13195 0.0 20.673079 10-11 >>END_MODULE